>AFG267 (AFG267Q) /CSM/AF/AFG2-C/AFG267Q.Seq.d/
ATTTTTCATATTTATATATAATATTACACCCCATAACTAATAAAATGTCATTCAATTCAA
GAATTGAAACTATTTCTCGTCACTTAAGCACTGGACCAATTTTAGAATCAAATCCAACTT
CTGGATCAGTTAAATCAGGAGATGATATTGTAATTGTTGCACCATTTCGTACAGCAATTT
GTAAAGCTAAAAGAGGAGCATTCAAAGAGACAGCACCAGATGATTTATTAGCACCAGTTA
TTAAACATATTATCAAAGTTACCAAATTAGATCCAAAATTAATTGGAGATGTTGTTATGG
GAGCAGTATTACCAAGATCATCACAAGGTGCAACCGAAGTTAGAGTTGCCACATTATTAG
CAGGTTTACCAAAGGAAGTACCATGTCATACAGTCAACAGACAATGTTCATCAGGTTTAC
AAGCAATTGCCAATTGTGCAGCAGCAGTCAAAGCAGGTTTCTATGACATTGGTTTAGCAG
GCGGTGTAGAATCAATGAGTTTAAATCCAATGGCATGGGATGGTGGTTTCAACGAGGTTG
CCCAAGAGGATCCAATCATTAGTGGTTGTTACAACACTATGGGTCAAACCTCTGAAAATG
TCGCAGGGCGTTTTGGTGTCACCAGAAAAGACCAAGATGAATTCTCTGTAAATTCACACA
AGAAGGCAGGTGCCGCCCAAAAAGCAGGTAAATTTGACGATGAAATCGTTCCAGTCACCG
TAAAGACCGAGGATGGCAAAACCATCGTCGTCGATAAAGACGAAGGTATCCGTGAGAAAA
CCACCGTTGCAGATTTGGCCAAATTAAAACCAGCTTTCAAAGAGGGTGGTACAACCACCG
CTGGTAACTCTAGTCAAATGAGTGATGGTGCCGCCGCTTGTTTAGTTATGAAAAGAAGTA
CCGCCGAAAGATTGGGTCTCAAGGTTGAATTGGTTTTCGTAGCTTTTGCTGCTGTCGGTG
TCGAACCATCCATTATGGGTATTGGTCCAGCCGTTGCAATTCCAGCCGCCTTAAAACAAG
CTGGTCTCACCACTAAAGATATCGACGTTTTTGAAATCAATGAAGCTTTTGCTTCTCAAG
CATTTTACAGTTGTAAGGTTTTGGGTTTGGATATGAACAAAGTTAATCCAAATGGCTCTG
GTATCGCTTTAGGTCATCCATTAGGTGCTACTGGTGCCCGTCAAATGGCTACCATTTCAA
GAGAATTACATCGTCGTAAAGGTAAATATGGTGTAATCTNCTATGTGTATTGGTACGGNC
TTAGGTGCTGCAGGCTGTTGTTCGTACAG

FSYLYIILHPITNKMSFNSRIETISRHLSTGPILESNPTSGSVKSGDDIVIVAPFRTAIC
KAKRGAFKETAPDDLLAPVIKHIIKVTKLDPKLIGDVVMGAVLPRSSQGATEVRVATLLA
GLPKEVPCHTVNRQCSSGLQAIANCAAAVKAGFYDIGLAGGVESMSLNPMAWDGGFNEVA
QEDPIISGCYNTMGQTSENVAGRFGVTRKDQDEFSVNSHKKAGAAQKAGKFDDEIVPVTV
KTEDGKTIVVDKDEGIREKTTVADLAKLKPAFKEGGTTTAGNSSQMSDGAAACLVMKRST
AERLGLKVELVFVAFAAVGVEPSIMGIGPAVAIPAALKQAGLTTKDIDVFEINEAFASQA
FYSCKVLGLDMNKVNPNGSGIALGHPLGATGARQMATISRELHRRKGKYGVIXYVYWYGL
RCCRLLFVQ

Frame A:
ifhiyi*yytp*likchsiqelklflvt*aldqf*nqiqlldqlnqemil*llhhfvqqf
vklkeehskrqhqmiy*hqllnilsklpn*iqn*lemllweqyyqdhhkvqpklelphy*
qvyqrkyhviqstdnvhqvykqlpivqqqskqvsmtlv*qav*nq*v*iqwhgmvvstrl
pkriqslvvvttlwvkplkmsqgvlvspektkmnsl*ihtrrqvppkkqvnltmksfqsp
*rprmakpsssiktkvsvrkpplqiwpn*nqlskrvvqpplvtlvk*vmvpplv*l*kev
ppkdwvsrlnwfs*llllsvsnhplwvlvqplqfqpp*nklvsplkistflksmkllllk
hftvvrfwvwi*tkliqmalvsl*vih*vllvpvkwlpfqenyivvkvnmv*sxmcigtx
lgaagccsy


Frame B:
ffifiynitphn**nviqfkn*nyfsslkhwtnfriksnfwis*irr*ycncctisysnl
*s*krsiqrdstr*fistsy*tyyqsyqirskinwrccygssitkiitrcnrs*schiis
rftkgstmsysqqtmfirftsncqlcsssqsrfl*hwfsrrcrinefksngmgwwfqrgc
prgsnh*wllqhygsnl*kcrrafwchqkrpr*ilckftqegrcrpksr*i*r*nrsshr
kdrgwqnhrrr*rrryp*enhrcrfgqiktsfqrgwynhrw*l*sne*wcrrlfsyekky
rrkigsqg*igfrsfcccrcrtihygywssrcnssrlktswshh*ryrrf*nq*sfcfss
ilql*gfgfgyeqs*skwlwyrfrssircywcpsngyhfkritss*r*iwcnllcvlvrx
*vlqavvrt


Frame C:
FSYLYIILHPITNKMSFNSRIETISRHLSTGPILESNPTSGSVKSGDDIVIVAPFRTAIC
KAKRGAFKETAPDDLLAPVIKHIIKVTKLDPKLIGDVVMGAVLPRSSQGATEVRVATLLA
GLPKEVPCHTVNRQCSSGLQAIANCAAAVKAGFYDIGLAGGVESMSLNPMAWDGGFNEVA
QEDPIISGCYNTMGQTSENVAGRFGVTRKDQDEFSVNSHKKAGAAQKAGKFDDEIVPVTV
KTEDGKTIVVDKDEGIREKTTVADLAKLKPAFKEGGTTTAGNSSQMSDGAAACLVMKRST
AERLGLKVELVFVAFAAVGVEPSIMGIGPAVAIPAALKQAGLTTKDIDVFEINEAFASQA
FYSCKVLGLDMNKVNPNGSGIALGHPLGATGARQMATISRELHRRKGKYGVIXYVYWYGL
RCCRLLFVQ

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFG267 (AFG267Q) /CSM/AF/AFG2-C/AFG267Q.Seq.d/                       2543   0.0
CFF662 (CFF662Q) /CSM/CF/CFF6-C/CFF662Q.Seq.d/                       2448   0.0
AFA747 (AFA747Q) /CSM/AF/AFA7-B/AFA747Q.Seq.d/                       2440   0.0
CFC445 (CFC445Q) /CSM/CF/CFC4-B/CFC445Q.Seq.d/                       2424   0.0
SFD792 (SFD792Q) /CSM/SF/SFD7-D/SFD792Q.Seq.d/                       2422   0.0
CFC529 (CFC529Q) /CSM/CF/CFC5-B/CFC529Q.Seq.d/                       2415   0.0
SFA524 (SFA524Q) /CSM/SF/SFA5-A/SFA524Q.Seq.d/                       2409   0.0
VFM636 (VFM636Q) /CSM/VF/VFM6-B/VFM636Q.Seq.d/                       2407   0.0
AFE219 (AFE219Q) /CSM/AF/AFE2-A/AFE219Q.Seq.d/                       2407   0.0
VFD865 (VFD865Q) /CSM/VF/VFD8-C/VFD865Q.Seq.d/                       2405   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.  2189  0.0  4
AU089542|AU089542.1 Lotus japonicus flower bud cDNA clone: Ljfb-v09.    44  7e-13  5
CB005315|CB005315.1 VVC011E10_126660 An expressed sequence tag database for abiotic stressed berries of Vitis vinifera var. Chardonnay Vitis vinifera cDNA clone VVC011E10 5, mRNA sequence.    60  3e-11  4
CB008288|CB008288.1 VVC055H06_148036 An expressed sequence tag database for abiotic stressed berries of Vitis vinifera var. Chardonnay Vitis vinifera cDNA clone VVC055H06 5, mRNA sequence.    60  1e-09  3
BM065622|BM065622.1 KS07005E12 KS07 Capsicum annuum cDNA, mRNA sequence.    68  1e-08  2
CA515447|CA515447.1 KS09042A06 KS09 Capsicum annuum cDNA, mRNA sequence.    68  1e-08  2
BM066045|BM066045.1 KS07010C02 KS07 Capsicum annuum cDNA, mRNA sequence.    68  2e-08  2
CF213585|CF213585.1 CGF1000794_F07 Vitis vinifera cv. cabernet sauvignon Stem - CAST Vitis vinifera cDNA clone CAST0006_IF_F07 5', mRNA sequence.    60  6e-08  3
AI563047|AI563047.1 EST00171 watermelon lambda zap library Citrullus lanatus cDNA clone WMLS336 5' similar to acetyl-CoA acyltransferase, mRNA sequence.    56  1e-07  2
BE642069|BE642069.1 Cri2_5_A22_SP6 Ceratopteris Spore Library Ceratopteris richardii cDNA clone Cri2_5_A22 5', mRNA sequence.    40  3e-07  5

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC116986_75(AC116986|pid:none) Dictyostelium discoideum chromoso...   751   0.0
X75329_1(X75329|pid:none) M.indica (Manila) THMF5 mRNA for 3-ket...   413   e-114
AM474896_9(AM474896|pid:none) Vitis vinifera contig VV79X001698....   411   e-113
S57792(S57792)acetyl-CoA C-acyltransferase (EC 2.3.1.16) B precu...   411   e-113
AB333797_1(AB333797|pid:none) Glycine max mRNA for peroxisomal 3...   406   e-112
T07989(T07989) acetyl-CoA C-acyltransferase (EC 2.3.1.16) precur...   405   e-111
(Q56WD9) RecName: Full=3-ketoacyl-CoA thiolase 2, peroxisomal;  ...   405   e-111
EU964831_1(EU964831|pid:none) Zea mays clone 281881 3-ketoacyl-C...   403   e-111
BT034496_1(BT034496|pid:none) Zea mays full-length cDNA clone ZM...   403   e-111
AX025481_1(AX025481|pid:none) Sequence 7 from Patent WO0032789.       402   e-110

 psg: 0.91  gvh: 0.40  alm: 0.28  top: 0.33  tms: 0.07  mit: 0.33  mip: 0.05
 nuc: 0.02  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.50  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.03  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: nuclear
	 20.0 %: cytoplasmic
	 16.0 %: endoplasmic reticulum
	 16.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: Golgi
	  4.0 %: peroxisomal
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system

>> prediction for AFG267 is nuc

>AFG267F.Seq
ATTTTTCATATTTATATATAATATTACACCCCATAACTAATAAAATGTCATTCAATTCAA
GAATTGAAACTATTTCTCGTCACTTAAGCACTGGACCAATTTTAGAATCAAATCCAACTT
CTGGATCAGTTAAATCAGGAGATGATATTGTAATTGTTGCACCATTTCGTACAGCAATTT
GTAAAGCTAAAAGAGGAGCATTCAAAGAGACAGCACCAGATGATTTATTAGCACCAGTTA
TTAAACATATTATCAAAGTTACCAAATTAGATCCAAAATTAATTGGAGATGTTGTTATGG
GAGCAGTATTACCAAGATCATCACAAGGTGCAACCGAAGTTAGAGTTGCCACATTATTAG
CAGGTTTACCAAAGGAAGTACCATGTCATACAGTCAACAGACAATGTTCATCAGGTTTAC
AAGCAATTGCCAATTGTGCAGCAGCAGTCAAAGCAGGTTTCTATGACATTGGTTTAGCAG
GCGGTGTAGAATCAATGAGTTTAAATCCAATGGCATGGGATGGTGGTTTCAACGAGGTTG
CCCAAGAGGATCCAATCATTAGTGGTTGTTACAACACTATGGGTCAAACCTCTGAAAATG
TCGCAGGGCGTTTTGGTGTCACCAGAAAAGACCAAGATGAATTCTCTGTAAATTC-----
-----

>AFG267Z.Seq
----------GATCCAATCATTAGTGGTTGTTACAACACTATGGGTCAAACCTCTGAAAA
TGTCGCAGGGCGTTTTGGTGTCACCAGAAAAGACCAAGATGAATTCTCTGTAAATTCACA
CAAGAAGGCAGGTGCCGCCCAAAAAGCAGGTAAATTTGACGATGAAATCGTTCCAGTCAC
CGTAAAGACCGAGGATGGCAAAACCATCGTCGTCGATAAAGACGAAGGTATCCGTGAGAA
AACCACCGTTGCAGATTTGGCCAAATTAAAACCAGCTTTCAAAGAGGGTGGTACAACCAC
CGCTGGTAACTCTAGTCAAATGAGTGATGGTGCCGCCGCTTGTTTAGTTATGAAAAGAAG
TACCGCCGAAAGATTGGGTCTCAAGGTTGAATTGGTTTTCGTAGCTTTTGCTGCTGTCGG
TGTCGAACCATCCATTATGGGTATTGGTCCAGCCGTTGCAATTCCAGCCGCCTTAAAACA
AGCTGGTCTCACCACTAAAGATATCGACGTTTTTGAAATCAATGAAGCTTTTGCTTCTCA
AGCATTTTACAGTTGTAAGGTTTTGGGTTTGGATATGAACAAAGTTAATCCAAATGGCTC
TGGTATCGCTTTAGGTCATCCATTAGGTGCTACTGGTGCCCGTCAAATGGCTACCATTTC
AAGAGAATTACATCGTCGTAAAGGTAAATATGGTGTAATCTNCTATGTGTATTGGTACGG
NCTTAGGTGCTGCAGGCTGTTGTTCGTACAG

>AFG267P.Seq
ATTTTTCATATTTATATATAATATTACACCCCATAACTAATAAAATGTCATTCAATTCAA
GAATTGAAACTATTTCTCGTCACTTAAGCACTGGACCAATTTTAGAATCAAATCCAACTT
CTGGATCAGTTAAATCAGGAGATGATATTGTAATTGTTGCACCATTTCGTACAGCAATTT
GTAAAGCTAAAAGAGGAGCATTCAAAGAGACAGCACCAGATGATTTATTAGCACCAGTTA
TTAAACATATTATCAAAGTTACCAAATTAGATCCAAAATTAATTGGAGATGTTGTTATGG
GAGCAGTATTACCAAGATCATCACAAGGTGCAACCGAAGTTAGAGTTGCCACATTATTAG
CAGGTTTACCAAAGGAAGTACCATGTCATACAGTCAACAGACAATGTTCATCAGGTTTAC
AAGCAATTGCCAATTGTGCAGCAGCAGTCAAAGCAGGTTTCTATGACATTGGTTTAGCAG
GCGGTGTAGAATCAATGAGTTTAAATCCAATGGCATGGGATGGTGGTTTCAACGAGGTTG
CCCAAGAGGATCCAATCATTAGTGGTTGTTACAACACTATGGGTCAAACCTCTGAAAATG
TCGCAGGGCGTTTTGGTGTCACCAGAAAAGACCAAGATGAATTCTCTGTAAATTC-----
-----GATCCAATCATTAGTGGTTGTTACAACACTATGGGTCAAACCTCTGAAAATGTCG
CAGGGCGTTTTGGTGTCACCAGAAAAGACCAAGATGAATTCTCTGTAAATTCACACAAGA
AGGCAGGTGCCGCCCAAAAAGCAGGTAAATTTGACGATGAAATCGTTCCAGTCACCGTAA
AGACCGAGGATGGCAAAACCATCGTCGTCGATAAAGACGAAGGTATCCGTGAGAAAACCA
CCGTTGCAGATTTGGCCAAATTAAAACCAGCTTTCAAAGAGGGTGGTACAACCACCGCTG
GTAACTCTAGTCAAATGAGTGATGGTGCCGCCGCTTGTTTAGTTATGAAAAGAAGTACCG
CCGAAAGATTGGGTCTCAAGGTTGAATTGGTTTTCGTAGCTTTTGCTGCTGTCGGTGTCG
AACCATCCATTATGGGTATTGGTCCAGCCGTTGCAATTCCAGCCGCCTTAAAACAAGCTG
GTCTCACCACTAAAGATATCGACGTTTTTGAAATCAATGAAGCTTTTGCTTCTCAAGCAT
TTTACAGTTGTAAGGTTTTGGGTTTGGATATGAACAAAGTTAATCCAAATGGCTCTGGTA
TCGCTTTAGGTCATCCATTAGGTGCTACTGGTGCCCGTCAAATGGCTACCATTTCAAGAG
AATTACATCGTCGTAAAGGTAAATATGGTGTAATCTNCTATGTGTATTGGTACGGNCTTA
GGTGCTGCAGGCTGTTGTTCGTACAG

>AFG267E.Seq
ATTTTTCATATTTATATATAATATTACACCCCATAACTAATAAAATGTCATTCAATTCAA
GAATTGAAACTATTTCTCGTCACTTAAGCACTGGACCAATTTTAGAATCAAATCCAACTT
CTGGATCAGTTAAATCAGGAGATGATATTGTAATTGTTGCACCATTTCGTACAGCAATTT
GTAAAGCTAAAAGAGGAGCATTCAAAGAGACAGCACCAGATGATTTATTAGCACCAGTTA
TTAAACATATTATCAAAGTTACCAAATTAGATCCAAAATTAATTGGAGATGTTGTTATGG
GAGCAGTATTACCAAGATCATCACAAGGTGCAACCGAAGTTAGAGTTGCCACATTATTAG
CAGGTTTACCAAAGGAAGTACCATGTCATACAGTCAACAGACAATGTTCATCAGGTTTAC
AAGCAATTGCCAATTGTGCAGCAGCAGTCAAAGCAGGTTTCTATGACATTGGTTTAGCAG
GCGGTGTAGAATCAATGAGTTTAAATCCAATGGCATGGGATGGTGGTTTCAACGAGGTTG
CCCAAGAGGATCCAATCATTAGTGGTTGTTACAACACTATGGGTCAAACCTCTGAAAATG
TCGCAGGGCGTTTTGGTGTCACCAGAAAAGACCAAGATGAATTCTCTGTAAATTCACACA
AGAAGGCAGGTGCCGCCCAAAAAGCAGGTAAATTTGACGATGAAATCGTTCCAGTCACCG
TAAAGACCGAGGATGGCAAAACCATCGTCGTCGATAAAGACGAAGGTATCCGTGAGAAAA
CCACCGTTGCAGATTTGGCCAAATTAAAACCAGCTTTCAAAGAGGGTGGTACAACCACCG
CTGGTAACTCTAGTCAAATGAGTGATGGTGCCGCCGCTTGTTTAGTTATGAAAAGAAGTA
CCGCCGAAAGATTGGGTCTCAAGGTTGAATTGGTTTTCGTAGCTTTTGCTGCTGTCGGTG
TCGAACCATCCATTATGGGTATTGGTCCAGCCGTTGCAATTCCAGCCGCCTTAAAACAAG
CTGGTCTCACCACTAAAGATATCGACGTTTTTGAAATCAATGAAGCTTTTGCTTCTCAAG
CATTTTACAGTTGTAAGGTTTTGGGTTTGGATATGAACAAAGTTAATCCAAATGGCTCTG
GTATCGCTTTAGGTCATCCATTAGGTGCTACTGGTGCCCGTCAAATGGCTACCATTTCAA
GAGAATTACATCGTCGTAAAGGTAAATATGGTGTAATCTNCTATGTGTATTGGTACGGNC
TTAGGTGCTGCAGGCTGTTGTTCGTACAG