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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CHI516
Library CH
(Link to library)
Clone ID CHI516
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U11477-1
Original site URL
Representative seq. ID CHI516P
(Link to Original site)
Representative DNA sequence 
>CHI516 (CHI516Q) /CSM/CH/CHI5-A/CHI516Q.Seq.d/
AAGTAGTTTGTAATTTCCGTACAATCGCACCAACAGCAGAATTTGATGATTGTACTCGTT
GGATCGATGGTTTGGAAGCTGCAAGAAAACGTTCAATTGATATCATTAAAATGTTCGGTA
TCAATGAAAAAGAAGTACTCTCACCATTACCATCATCATCATCAATTCCATCATCTGGTA
GTAATGGTAATTTATTGGATCCATCAGCTGATGATTTAACACGTTATTTGGATTTAACAG
CAATTAAAAATGGTAAACAAAAGATTTTAATTCAAGTTAAAGGTAAAAGAAAGATTAGAG
TTAGAATGGTTAAATTATCAACATCATCATTGAATTGTCATAACTCTTTTATTTTAGATG
CTGGTCCAAGAATTTTCGTCTGGGCAGGTTCAAAAACAAGTCGTGTCAATAAAGCAAAGG
CATTAGATTTTGCAAATAGAATTCGTACAAAAGAAAGAGGTGGTAAATCAACACTAATTC
AATTGGATCAAGGTCGTGATGAACAATCATTTGATTTTTGGGAAATTTTAGGTGGTAATA
GTTCCGATCCAATCGCAACAACTCCAACTCCAXXXXXXXXXXGAGAATTTCAATTCCACT
TCAGCTCCAATCGAAATCTTAAAAAGAAGGTAGTGAATCCAATGAATTTTGGTCAGCTTT
TGAAAGTACAGGGTGGTAGACAAAAATACTTTAATGATATTATGATTCAAAGTAGTTCAA
TTCCAACCTCATTCACTTATAAACCACGTTTCTTTGTTTGTAGTAATGCATCGGGTATCG
TTGAAGTTACAGAGGAATCACCATTCTCTCAAGATGATCTAGATATTGGATCCGTTTGTA
TATTGGATGTACAAAGTCATATTTACCTTTGGATTGGTAGTCGTGCAACTCATCGTACTA
AACGTGCCTCTATGGAAGTTGTATTAAACTTTATTGAAACTTCAAAATTGGGTCATTCAA
AAGAGCACACTAAAGTACTAATTGCAACTCCATTCGAAGAACCAATTGGTTTCAAAAGTT
ATTTCCGTGCTTGGTGTACCTCTAAATATCCAAAGAATAAATTACCTTTGGTTGAAAAAG
ATGGTATCCCAGTTGAACAAGTACTTAAAGATTATCTCAAAGAAATCTATACCTATGAAG
AGTTATTGGCCGATCCATTACCTGCTGGCGTTGACTCTACTAAATTGGATACTTATCTCA
ATGATGAAGATTTTGAAAAAGTTTTCAAAATGACTAGAACTGAATGGTTAAAGATTCCAG
CTTGGAAGAGAGAAGGTATTAAAAAAGAATTATTCTTATTTTAAATAATAAAAATAATCT
CTATCTATAATATACATTCATATAA
sequence update 2002.10.25
Translated Amino Acid sequence 
VVCNFRTIAPTAEFDDCTRWIDGLEAARKRSIDIIKMFGINEKEVLSPLPSSSSIPSSGS
NGNLLDPSADDLTRYLDLTAIKNGKQKILIQVKGKRKIRVRMVKLSTSSLNCHNSFILDA
GPRIFVWAGSKTSRVNKAKALDFANRIRTKERGGKSTLIQLDQGRDEQSFDFWEILGGNS
SDPIATTPTP---

---EFQFHFSSNRNLKKKVVNPMNFGQLLKVQGGRQKYFNDIMIQSSSIPTSFTYKPRFF
VCSNASGIVEVTEESPFSQDDLDIGSVCILDVQSHIYLWIGSRATHRTKRASMEVVLNFI
ETSKLGHSKEHTKVLIATPFEEPIGFKSYFRAWCTSKYPKNKLPLVEKDGIPVEQVLKDY
LKEIYTYEELLADPLPAGVDSTKLDTYLNDEDFEKVFKMTRTEWLKIPAWKREGIKKELF
LF*iikiisiynihsy
Translated Amino Acid sequence (All Frames) 
Frame A:
k*fvisvqshqqqnlmivlvgsmvwklqenvqlislkcsvsmkkkyshhyhhhhqfhhlv
vmviywihqlmi*hviwi*qqlkmvnkrf*fklkvkerlelewlnyqhhh*ivitllf*m
lvqefssgqvqkqvvsikqrh*ilqiefvqkkevvnqh*fnwikvvmnnhlifgkf*vvi
vpiqsqqlql---

---enfnstsapieilkrr**iq*ilvsf*kyrvvdkntlmil*fkvvqfqphslinhvs
lfvvmhrvslklqrnhhslkmi*ildpfvywmykviftfglvvvqlivlnvplwkly*tl
lklqnwviqkstlky*lqlhsknqlvskvisvlgvplniqrinylwlkkmvsqlnkylki
iskksipmksywpihyllaltllnwilismmkilkkfsk*lelng*rfqlgrekvlkkny
syfk**k*slsiiyihi

Frame B:
ssl*fpynrtnsri**lysldrwfgsckktfn*yh*nvryq*krstltitiiiinsiiw*
*w*figsis**fntlfgfnsn*kw*tkdfnss*r*kkd*s*ng*iiniiiels*lfyfrc
wsknfrlgrfknkscq*skgirfck*nsykrkrw*intnsigsrs**tii*flgnfrw**
frsnrnnsns---

---risiplqlqsks*kegsesnefwsafestgw*tkil**yydsk*fnsnlihl*ttfl
cl**cigyr*syrgitilsr*srywirlyigctksylpldw*scnssy*tclygscikly
*nfkigsfkrah*stncnsirrtnwfqklfpclvyl*iske*itfg*krwyps*tst*rl
sqrnlyl*rvigrsitcwr*ly*igylsq**rf*ksfqnd*n*mvkdssleerry*krii
lilnnknnlyl*ytfi*

Frame C:
VVCNFRTIAPTAEFDDCTRWIDGLEAARKRSIDIIKMFGINEKEVLSPLPSSSSIPSSGS
NGNLLDPSADDLTRYLDLTAIKNGKQKILIQVKGKRKIRVRMVKLSTSSLNCHNSFILDA
GPRIFVWAGSKTSRVNKAKALDFANRIRTKERGGKSTLIQLDQGRDEQSFDFWEILGGNS
SDPIATTPTP---

---EFQFHFSSNRNLKKKVVNPMNFGQLLKVQGGRQKYFNDIMIQSSSIPTSFTYKPRFF
VCSNASGIVEVTEESPFSQDDLDIGSVCILDVQSHIYLWIGSRATHRTKRASMEVVLNFI
ETSKLGHSKEHTKVLIATPFEEPIGFKSYFRAWCTSKYPKNKLPLVEKDGIPVEQVLKDY
LKEIYTYEELLADPLPAGVDSTKLDTYLNDEDFEKVFKMTRTEWLKIPAWKREGIKKELF
LF*iikiisiynihsy
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHI516 (CHI516Q) /CSM/CH/CHI5-A/CHI516Q.Seq.d/                       2607   0.0
SHK802 (SHK802Q) /CSM/SH/SHK8-A/SHK802Q.Seq.d/                       1419   0.0
AHI567 (AHI567Q) /CSM/AH/AHI5-C/AHI567Q.Seq.d/                       1407   0.0
AFI116 (AFI116Q) /CSM/AF/AFI1-A/AFI116Q.Seq.d/                       1386   0.0
SHJ849 (SHJ849Q) /CSM/SH/SHJ8-C/SHJ849Q.Seq.d/                       1370   0.0
AHN388 (AHN388Q) /CSM/AH/AHN3-D/AHN388Q.Seq.d/                       1285   0.0
SSB880 (SSB880Q) /CSM/SS/SSB8-D/SSB880Q.Seq.d/                       1213   0.0
AHC823 (AHC823Q) /CSM/AH/AHC8-A/AHC823Q.Seq.d/                       1150   0.0
AHC554 (AHC554Q) /CSM/AH/AHC5-C/AHC554Q.Seq.d/                       1132   0.0
AHF158 (AHF158Q) /CSM/AH/AHF1-C/AHF158Q.Seq.d/                       1102   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U78754|U78754.1 Dictyostelium discoideum villin (vilA) mRNA, partial cds.  1336   0.0    3
AJ427856|AJ427856.1 Dictyostelium discoideum ORF encoding villidin.  1255   0.0    3
AC114263|AC114263.2 Dictyostelium discoideum chromosome 2 map 215673-367476 strain AX4, complete sequence.    46   7e-09  13
AC116551|AC116551.2 Dictyostelium discoideum chromosome 2 map complement(581327-427576) strain AX4, complete sequence.    40   1e-08  14
AC116956|AC116956.2 Dictyostelium discoideum chromosome 2 map 1418423-1684967 strain AX4, complete sequence.    48   2e-08  18
AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.    38   2e-07  16
AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.    44   6e-07  23
AC116979|AC116979.3 Dictyostelium discoideum chromosome 2 map 6445720-6776760 strain AX4, complete sequence.    40   7e-07  18
AC115598|AC115598.2 Dictyostelium discoideum chromosome 2 map 581427-735498 strain AX4, complete sequence.    34   1e-05  14
AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.    36   1e-05  23
dna update 2004.12. 6
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AJ427856_1(AJ427856|pid:none) Dictyostelium discoideum ORF encod...   424   e-117
(Q8WQ85) RecName: Full=Villidin;                                      424   e-117
BC135895_1(BC135895|pid:none) Xenopus tropicalis hypothetical pr...   107   7e-22
DQ453501_1(DQ453501|pid:none) Heliocidaris erythrogramma advilli...   107   9e-22
DQ453500_1(DQ453500|pid:none) Heliocidaris tuberculata advillin ...   105   3e-21
BC054960_1(BC054960|pid:none) Xenopus laevis villin 1, mRNA (cDN...   105   3e-21
EU334660_1(EU334660|pid:none) Strongylocentrotus purpuratus vill...   104   6e-21
DQ453502_1(DQ453502|pid:none) Strongylocentrotus purpuratus advi...   104   8e-21
AE014297_18(AE014297|pid:none) Drosophila melanogaster chromosom...   103   1e-20
(Q07171) RecName: Full=Gelsolin; Flags: Precursor;  &AE014297_15...   103   1e-20
protein update 2009. 4. 2
PSORT 

 psg: 0.80  gvh: 0.49  alm: 0.40  top: 0.53  tms: 0.00  mit: 0.24  mip: 0.02
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 32.0 %: cytoplasmic
	 32.0 %: nuclear
	 20.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: Golgi
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for CHI516 is cyt
5' end seq. ID CHI516F
5' end seq. 
>CHI516F.Seq
AAGTAGTTTGTAATTTCCGTACAATCGCACCAACAGCAGAATTTGATGATTGTACTCGTT
GGATCGATGGTTTGGAAGCTGCAAGAAAACGTTCAATTGATATCATTAAAATGTTCGGTA
TCAATGAAAAAGAAGTACTCTCACCATTACCATCATCATCATCAATTCCATCATCTGGTA
GTAATGGTAATTTATTGGATCCATCAGCTGATGATTTAACACGTTATTTGGATTTAACAG
CAATTAAAAATGGTAAACAAAAGATTTTAATTCAAGTTAAAGGTAAAAGAAAGATTAGAG
TTAGAATGGTTAAATTATCAACATCATCATTGAATTGTCATAACTCTTTTATTTTAGATG
CTGGTCCAAGAATTTTCGTCTGGGCAGGTTCAAAAACAAGTCGTGTCAATAAAGCAAAGG
CATTAGATTTTGCAAATAGAATTCGTACAAAAGAAAGAGGTGGTAAATCAACACTAATTC
AATTGGATCAAGGTCGTGATGAACAATCATTTGATTTTTGGGAAATTTTAGGTGGTAATA
GTTCCGATCCAATCGCAACAACTCCAACTCCANNNNNNNNNN
Length of 5' end seq. 582
3' end seq. ID CHI516Z
3' end seq. 
>CHI516Z.Seq
NNNNNNNNNNGAGAATTTCAATTCCACTTCAGCTCCAATCGAAATCTTAAAAAGAAGGTA
GTGAATCCAATGAATTTTGGTCAGCTTTTGAAAGTACAGGGTGGTAGACAAAAATACTTT
AATGATATTATGATTCAAAGTAGTTCAATTCCAACCTCATTCACTTATAAACCACGTTTC
TTTGTTTGTAGTAATGCATCGGGTATCGTTGAAGTTACAGAGGAATCACCATTCTCTCAA
GATGATCTAGATATTGGATCCGTTTGTATATTGGATGTACAAAGTCATATTTACCTTTGG
ATTGGTAGTCGTGCAACTCATCGTACTAAACGTGCCTCTATGGAAGTTGTATTAAACTTT
ATTGAAACTTCAAAATTGGGTCATTCAAAAGAGCACACTAAAGTACTAATTGCAACTCCA
TTCGAAGAACCAATTGGTTTCAAAAGTTATTTCCGTGCTTGGTGTACCTCTAAATATCCA
AAGAATAAATTACCTTTGGTTGAAAAAGATGGTATCCCAGTTGAACAAGTACTTAAAGAT
TATCTCAAAGAAATCTATACCTATGAAGAGTTATTGGCCGATCCATTACCTGCTGGCGTT
GACTCTACTAAATTGGATACTTATCTCAATGATGAAGATTTTGAAAAAGTTTTCAAAATG
ACTAGAACTGAATGGTTAAAGATTCCAGCTTGGAAGAGAGAAGGTATTAAAAAAGAATTA
TTCTTATTTTAAATAATAAAAATAATCTCTATCTATAATATACATTCATATAA
Length of 3' end seq. 773
Connected seq. ID CHI516P
Connected seq. 
>CHI516P.Seq
AAGTAGTTTGTAATTTCCGTACAATCGCACCAACAGCAGAATTTGATGATTGTACTCGTT
GGATCGATGGTTTGGAAGCTGCAAGAAAACGTTCAATTGATATCATTAAAATGTTCGGTA
TCAATGAAAAAGAAGTACTCTCACCATTACCATCATCATCATCAATTCCATCATCTGGTA
GTAATGGTAATTTATTGGATCCATCAGCTGATGATTTAACACGTTATTTGGATTTAACAG
CAATTAAAAATGGTAAACAAAAGATTTTAATTCAAGTTAAAGGTAAAAGAAAGATTAGAG
TTAGAATGGTTAAATTATCAACATCATCATTGAATTGTCATAACTCTTTTATTTTAGATG
CTGGTCCAAGAATTTTCGTCTGGGCAGGTTCAAAAACAAGTCGTGTCAATAAAGCAAAGG
CATTAGATTTTGCAAATAGAATTCGTACAAAAGAAAGAGGTGGTAAATCAACACTAATTC
AATTGGATCAAGGTCGTGATGAACAATCATTTGATTTTTGGGAAATTTTAGGTGGTAATA
GTTCCGATCCAATCGCAACAACTCCAACTCCA----------GAGAATTTCAATTCCACT
TCAGCTCCAATCGAAATCTTAAAAAGAAGGTAGTGAATCCAATGAATTTTGGTCAGCTTT
TGAAAGTACAGGGTGGTAGACAAAAATACTTTAATGATATTATGATTCAAAGTAGTTCAA
TTCCAACCTCATTCACTTATAAACCACGTTTCTTTGTTTGTAGTAATGCATCGGGTATCG
TTGAAGTTACAGAGGAATCACCATTCTCTCAAGATGATCTAGATATTGGATCCGTTTGTA
TATTGGATGTACAAAGTCATATTTACCTTTGGATTGGTAGTCGTGCAACTCATCGTACTA
AACGTGCCTCTATGGAAGTTGTATTAAACTTTATTGAAACTTCAAAATTGGGTCATTCAA
AAGAGCACACTAAAGTACTAATTGCAACTCCATTCGAAGAACCAATTGGTTTCAAAAGTT
ATTTCCGTGCTTGGTGTACCTCTAAATATCCAAAGAATAAATTACCTTTGGTTGAAAAAG
ATGGTATCCCAGTTGAACAAGTACTTAAAGATTATCTCAAAGAAATCTATACCTATGAAG
AGTTATTGGCCGATCCATTACCTGCTGGCGTTGACTCTACTAAATTGGATACTTATCTCA
ATGATGAAGATTTTGAAAAAGTTTTCAAAATGACTAGAACTGAATGGTTAAAGATTCCAG
CTTGGAAGAGAGAAGGTATTAAAAAAGAATTATTCTTATTTTAAATAATAAAAATAATCT
CTATCTATAATATACATTCATATAA
Length of connected seq. 1335
Full length Seq ID -
Full length Seq. -
Length of full length seq. -