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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFG245
Library AF
(Link to library)
Clone ID AFG245
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID AFG245P
(Link to Original site)
Representative DNA sequence 
>AFG245 (AFG245Q) /CSM/AF/AFG2-B/AFG245Q.Seq.d/
ATTTATAATTATATCTTTTTTGTTTAATAAAAAAGAAAGAATACCAACATGAGACTTTTA
TTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATTTTGCATTATCAACAGTTCATTTCAAA
GATACCTTTGATAACGATTGGGAATCAAGATGGGTTGTTAGTGATTGGCATAAAGAAGAT
GGTAAATCAGGTAAATTAGTTCATACTGCAGGTAAATGGTTTGGTGATGAAAATCAAAAA
GGTATTCAAACTAGTGAAGATGCTCGTTTCTACGCTGTATCAGCTAAATTCCCAAGTTTC
AGTAACAAAGGTAAAGATTTAGTTCTCCAATACACTGTAAAGAATGAACAAAAAGTTGAT
TGTGGTGGTTCATACATCAAGTTATTACCATCCAAATTAGATCAATCAGCATTTGATGGT
GAATCTGAATATTCCATTATGTTTGGTCCAGATGTTTGTGGTGCCTCAAAGAGAGTTCAC
GTCATTCTCAACTACAAAGGAAAGAACCATTTAATCAAGAAAGAAATCAACAAAGTTGAA
ACCGATCAATTAACTCATCAATACACTTTGGTCATTTCACCAGATAACACCTACAAAGTA
CTCGTCGATAACAAAGAGATCCAAGCAGGTAACCTCGCTGATGATXXXXXXXXXXCAGCT
TCAATCGTTGATCCAGAAGCTGTCAAACCAGAGGATTGGAATGAAGAAGACGACGGTGAA
TGGGAAGCCCCAACCATCGCCAATCCAGAATACAAAGGTGAATGGAAAGCAAAGAAAATC
CCAAATCCAGAATACAAAGGTGAATGGGTACACCCATTAATTGATAACCCAGAATACGCT
GAAGACAATGAACTCTACCTCTTTAATGATCTCGGTGCTATCGGTTTCGAATTATGGCAA
GTTAAATCTGGTTCCATCTTTAACAATATGATCGTCACCGACTCTGTCGAAGAAGCTAAA
GATTTCTCCGAAAAGACCTTTGTTGCCAACCAAGAAGCCGAAAAGAAAATGTTTGACGAC
CTCGAAGCCGCCAAAGCTGAAGAGAGAAAGAAAGCTGACGAAAAATTAGCTGCCGAAAAA
GCTGCTGAAAAAGAAGCCGAAGAAGCTGATGAAGAAGAAGAAGAAGTCGCTGAAGAAGAT
TTAGTTAAAACTGATGACAAAAAAGAAGAAGTCAAAAAATCAACCAAAAAAGTTGATCAT
GACGAACTTTAAANTAAATAAATAAATAATAAAAAAAAACAAATAAAAAAAAACTCGT
sequence update 2001. 6. 2
Translated Amino Acid sequence 
iynyiffv**KRKNTNMRLLLCLIFLVFVFNFALSTVHFKDTFDNDWESRWVVSDWHKED
GKSGKLVHTAGKWFGDENQKGIQTSEDARFYAVSAKFPSFSNKGKDLVLQYTVKNEQKVD
CGGSYIKLLPSKLDQSAFDGESEYSIMFGPDVCGASKRVHVILNYKGKNHLIKKEINKVE
TDQLTHQYTLVISPDNTYKVLVDNKEIQAGNLADD---

---ASIVDPEAVKPEDWNEEDDGEWEAPTIANPEYKGEWKAKKIPNPEYKGEWVHPLIDN
PEYAEDNELYLFNDLGAIGFELWQVKSGSIFNNMIVTDSVEEAKDFSEKTFVANQEAEKK
MFDDLEAAKAEERKKADEKLAAEKAAEKEAEEADEEEEEVAEEDLVKTDDKKEEVKKSTK
KVDHDEL*xk*innkkkqikkns
Translated Amino Acid sequence (All Frames) 
Frame A:
iynyiffv**KRKNTNMRLLLCLIFLVFVFNFALSTVHFKDTFDNDWESRWVVSDWHKED
GKSGKLVHTAGKWFGDENQKGIQTSEDARFYAVSAKFPSFSNKGKDLVLQYTVKNEQKVD
CGGSYIKLLPSKLDQSAFDGESEYSIMFGPDVCGASKRVHVILNYKGKNHLIKKEINKVE
TDQLTHQYTLVISPDNTYKVLVDNKEIQAGNLADD---

---qlqsliqklsnqrigmkkttvngkpqpspiqntkvngkqrksqiqntkvngyth*li
tqntlktmnstslmisvlsvsnygklnlvpslti*ssptlskklkispkrpllptkkpkr
kclttskppklkrerkltkn*lpkkllkkkpkklmkkkkkslkki*lklmtkkkksknqp
kklimtnfkxnk*iikknk*kktr

Frame B:
fiiisflfnkkeript*dfycv*fs*flfsilhyqqfiskiplitignqdgllvigikkm
vnqvn*filqvnglvmkikkvfklvkmlvstlyqlnsqvsvtkvki*fsntl*rmnkkli
vvvhtssyyhpn*inqhlmvnlniplclvqmfvvpqreftsfsttkerti*srkkstklk
pin*lintlwsfhqitptkyssitkrskqvtslm---

---sfnr*srscqtrgle*rrrr*mgspnhrqsriqr*meskenpksriqr*mgtpin**
prir*rq*tlpl**srcyrfrimas*iwfhl*qydrhrlcrrs*rflrkdlccqprsrke
nv*rprsrqs*rekes*rkiscrksc*krsrrs**rrrrsr*rrfs*n**qkrrsqkinq
ks*s*rtlx*ink**kktnkkkl

Frame C:
l*lylfclikkkeyqhetfivfnflsfcfqfciinssfqryl**rlgikmgc**la*rrw
*ir*issycr*mvw**kskrysn**rcsflrcis*ipkfq*qr*rfsspihcke*tks*l
wwfihqvitiqirsisi*w*i*ifhyvwsrclwclkessrhsqlqrkepfnqernqqs*n
rsinssihfghftr*hlqstrr*qrdpsr*pr**---

---ASIVDPEAVKPEDWNEEDDGEWEAPTIANPEYKGEWKAKKIPNPEYKGEWVHPLIDN
PEYAEDNELYLFNDLGAIGFELWQVKSGSIFNNMIVTDSVEEAKDFSEKTFVANQEAEKK
MFDDLEAAKAEERKKADEKLAAEKAAEKEAEEADEEEEEVAEEDLVKTDDKKEEVKKSTK
KVDHDEL*xk*innkkkqikkns
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFG245 (AFG245Q) /CSM/AF/AFG2-B/AFG245Q.Seq.d/                       1846   0.0
VFD485 (VFD485Q) /CSM/VF/VFD4-D/VFD485Q.Seq.d/                       1080   0.0
VFD121 (VFD121Q) /CSM/VF/VFD1-A/VFD121Q.Seq.d/                       1080   0.0
SLE570 (SLE570Q) /CSM/SL/SLE5-C/SLE570Q.Seq.d/                       1080   0.0
SLE160 (SLE160Q) /CSM/SL/SLE1-C/SLE160Q.Seq.d/                       1080   0.0
AFN528 (AFN528Q) /CSM/AF/AFN5-B/AFN528Q.Seq.d/                       1080   0.0
AFK716 (AFK716Q) /CSM/AF/AFK7-A/AFK716Q.Seq.d/                       1080   0.0
AFK286 (AFK286Q) /CSM/AF/AFK2-D/AFK286Q.Seq.d/                       1080   0.0
AFK245 (AFK245Q) /CSM/AF/AFK2-B/AFK245Q.Seq.d/                       1080   0.0
AFJ570 (AFJ570Q) /CSM/AF/AFJ5-C/AFJ570Q.Seq.d/                       1080   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U36937|U36937.1 Dictyostelium discoideum calreticulin mRNA, complete cds.  1072   0.0    4
BD248385|BD248385.1 Gene participating in tolerance against environmental stress.    92   3e-23  3
BH160872|BH160872.1 ENTRI18TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    80   3e-17  3
AZ543757|AZ543757.1 ENTGD24TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    80   9e-11  1
BI921109|BI921109.1 EST541012 tomato callus Lycopersicon esculentum cDNA clone cLEC72A21 5' end, mRNA sequence.    74   2e-10  2
CK247437|CK247437.1 EST731074 potato callus cDNA library, normalized and full-length Solanum tuberosum cDNA clone POCAT37 5' end, mRNA sequence.    66   4e-09  3
CK253744|CK253744.1 EST737381 potato callus cDNA library, normalized and full-length Solanum tuberosum cDNA clone POCC781 5' end, mRNA sequence.    66   4e-09  3
AW649196|AW649196.1 EST327650 tomato germinating seedlings, TAMU Lycopersicon esculentum cDNA clone cLEI7M10 5', mRNA sequence.    74   6e-09  1
AW626316|AW626316.1 EST320223 tomato radicle, 5 d post-imbibition, Cornell University Lycopersicon esculentum cDNA clone cLEZ19K24 5', mRNA sequence.    74   6e-09  1
BE459798|BE459798.1 EST415090 tomato developing/immature green fruit Lycopersicon esculentum cDNA clone cLEM8G13, mRNA sequence.    74   6e-09  1
dna update 2004. 5. 7
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U36937_1(U36937|pid:none) Dictyostelium discoideum calreticulin ...   573   e-162
AC133339_28(AC133339|pid:none) Oryza sativa chromosome 3 BAC OSJ...   343   7e-93
AB196794_1(AB196794|pid:none) Glycine max Gm crt-1 mRNA for calr...   337   4e-91
EF085019_1(EF085019|pid:none) Picea sitchensis clone WS0297_I16 ...   337   7e-91
(Q40401) RecName: Full=Calreticulin; Flags: Precursor;  &T16968(...   337   7e-91
AF283816_1(AF283816|pid:none) Pinus taeda calreticulin mRNA, com...   336   9e-91
(Q9XF98) RecName: Full=Calreticulin; Flags: Precursor;  &AF13473...   335   1e-90
EU984501_1(EU984501|pid:none) Nicotiana tabacum calreticulin mRN...   335   2e-90
JH0795(JH0795;B31409;F60977) calreticulin precursor - California...   334   3e-90
EF146676_1(EF146676|pid:none) Populus trichocarpa clone WS01211_...   334   4e-90
protein update 2009. 4.20
PSORT 

 psg: 1.01  gvh: 0.69  alm: 0.47  top: 0.10  tms: 0.00  mit: 0.40  mip: 0.03
 nuc: 0.06  erl: 1.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: extracellular, including cell wall
	 32.0 %: endoplasmic reticulum
	 12.0 %: cytoplasmic
	 12.0 %: vacuolar
	  4.0 %: Golgi
	  4.0 %: nuclear

>> prediction for AFG245 is exc
5' end seq. ID AFG245F
5' end seq. 
>AFG245F.Seq
ATTTATAATTATATCTTTTTTGTTTAATAAAAAAGAAAGAATACCAACATGAGACTTTTA
TTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATTTTGCATTATCAACAGTTCATTTCAAA
GATACCTTTGATAACGATTGGGAATCAAGATGGGTTGTTAGTGATTGGCATAAAGAAGAT
GGTAAATCAGGTAAATTAGTTCATACTGCAGGTAAATGGTTTGGTGATGAAAATCAAAAA
GGTATTCAAACTAGTGAAGATGCTCGTTTCTACGCTGTATCAGCTAAATTCCCAAGTTTC
AGTAACAAAGGTAAAGATTTAGTTCTCCAATACACTGTAAAGAATGAACAAAAAGTTGAT
TGTGGTGGTTCATACATCAAGTTATTACCATCCAAATTAGATCAATCAGCATTTGATGGT
GAATCTGAATATTCCATTATGTTTGGTCCAGATGTTTGTGGTGCCTCAAAGAGAGTTCAC
GTCATTCTCAACTACAAAGGAAAGAACCATTTAATCAAGAAAGAAATCAACAAAGTTGAA
ACCGATCAATTAACTCATCAATACACTTTGGTCATTTCACCAGATAACACCTACAAAGTA
CTCGTCGATAACAAAGAGATCCAAGCAGGTAACCTCGCTGATGAT----------
Length of 5' end seq. 645
3' end seq. ID AFG245Z
3' end seq. 
>AFG245Z.Seq
----------CAGCTTCAATCGTTGATCCAGAAGCTGTCAAACCAGAGGATTGGAATGAA
GAAGACGACGGTGAATGGGAAGCCCCAACCATCGCCAATCCAGAATACAAAGGTGAATGG
AAAGCAAAGAAAATCCCAAATCCAGAATACAAAGGTGAATGGGTACACCCATTAATTGAT
AACCCAGAATACGCTGAAGACAATGAACTCTACCTCTTTAATGATCTCGGTGCTATCGGT
TTCGAATTATGGCAAGTTAAATCTGGTTCCATCTTTAACAATATGATCGTCACCGACTCT
GTCGAAGAAGCTAAAGATTTCTCCGAAAAGACCTTTGTTGCCAACCAAGAAGCCGAAAAG
AAAATGTTTGACGACCTCGAAGCCGCCAAAGCTGAAGAGAGAAAGAAAGCTGACGAAAAA
TTAGCTGCCGAAAAAGCTGCTGAAAAAGAAGCCGAAGAAGCTGATGAAGAAGAAGAAGAA
GTCGCTGAAGAAGATTTAGTTAAAACTGATGACAAAAAAGAAGAAGTCAAAAAATCAACC
AAAAAAGTTGATCATGACGAACTTTAAANTAAATAAATAAATAATAAAAAAAAACAAATA
AAAAAAAACTCGT
Length of 3' end seq. 603
Connected seq. ID AFG245P
Connected seq. 
>AFG245P.Seq
ATTTATAATTATATCTTTTTTGTTTAATAAAAAAGAAAGAATACCAACATGAGACTTTTA
TTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATTTTGCATTATCAACAGTTCATTTCAAA
GATACCTTTGATAACGATTGGGAATCAAGATGGGTTGTTAGTGATTGGCATAAAGAAGAT
GGTAAATCAGGTAAATTAGTTCATACTGCAGGTAAATGGTTTGGTGATGAAAATCAAAAA
GGTATTCAAACTAGTGAAGATGCTCGTTTCTACGCTGTATCAGCTAAATTCCCAAGTTTC
AGTAACAAAGGTAAAGATTTAGTTCTCCAATACACTGTAAAGAATGAACAAAAAGTTGAT
TGTGGTGGTTCATACATCAAGTTATTACCATCCAAATTAGATCAATCAGCATTTGATGGT
GAATCTGAATATTCCATTATGTTTGGTCCAGATGTTTGTGGTGCCTCAAAGAGAGTTCAC
GTCATTCTCAACTACAAAGGAAAGAACCATTTAATCAAGAAAGAAATCAACAAAGTTGAA
ACCGATCAATTAACTCATCAATACACTTTGGTCATTTCACCAGATAACACCTACAAAGTA
CTCGTCGATAACAAAGAGATCCAAGCAGGTAACCTCGCTGATGAT----------CAGCT
TCAATCGTTGATCCAGAAGCTGTCAAACCAGAGGATTGGAATGAAGAAGACGACGGTGAA
TGGGAAGCCCCAACCATCGCCAATCCAGAATACAAAGGTGAATGGAAAGCAAAGAAAATC
CCAAATCCAGAATACAAAGGTGAATGGGTACACCCATTAATTGATAACCCAGAATACGCT
GAAGACAATGAACTCTACCTCTTTAATGATCTCGGTGCTATCGGTTTCGAATTATGGCAA
GTTAAATCTGGTTCCATCTTTAACAATATGATCGTCACCGACTCTGTCGAAGAAGCTAAA
GATTTCTCCGAAAAGACCTTTGTTGCCAACCAAGAAGCCGAAAAGAAAATGTTTGACGAC
CTCGAAGCCGCCAAAGCTGAAGAGAGAAAGAAAGCTGACGAAAAATTAGCTGCCGAAAAA
GCTGCTGAAAAAGAAGCCGAAGAAGCTGATGAAGAAGAAGAAGAAGTCGCTGAAGAAGAT
TTAGTTAAAACTGATGACAAAAAAGAAGAAGTCAAAAAATCAACCAAAAAAGTTGATCAT
GACGAACTTTAAANTAAATAAATAAATAATAAAAAAAAACAAATAAAAAAAAACTCGT
Length of connected seq. 1248
Full length Seq ID -
Full length Seq. -
Length of full length seq. -