>CHI458 (CHI458Q) /CSM/CH/CHI4-C/CHI458Q.Seq.d/
AATTAAATTATTTTTTAAAAGCAGTAATTCCAGCACAACATAAAATTAAAAGTGCACCTA
AAATTGTAATCCAATCGAGAGGTTGATGCATTAAAGTAATTTGCCAAATAAAAGTGAAAA
TTATTTGAAGGTAATTCATAGCAGCGGCAGTTGAAGTTTTTTTCGATAATTGAATACCAG
CATTTACAGCACCCTGAGCAACGGTAGCTACAACACCAATCAATGTGATATAACCCCAAG
CTTTAAGTGATGGCCAAACAAATGTTTGAAAAGCAAAAGAAGAAGGTAAACAAAGGGCAG
AGGAAACAGCACTGAAATAAATAACCAAAACAAAGGGGTTAACAGATGGACCAACTTTTC
TAACTGCAATATAGGAAACTGCTGTAAAGAAAGCACCACCAACACCAATCAATACATATA
ATAGCTTGCCACTGTCTTCACCATTTTCAGTGGTGGAACCATCGGTATGGTGATGGAATA
AGAATGATGGTTTGGAGACTACCACAACACCACATAAACTTAAAAATGCACAAATTGCCT
CGATTCCACCCCATTTCTCTTTAAGTACAACAGCTGCCAAGGCTGCAGTAATAACAGGAC
TTGTAAAACTAATGATTACAGCCXXXXXXXXXXCCTGTAGATTGATTAATAATTGATGAT
TTTTGAATACCACTATTACTCTTATTGATTATAACTGAATTATTANCTGGACTTGAAACT
AATGATGATGATGATGGTGATAATACTGAAAAAGTTGAAANTAAAGGTAAAATGGTACAA
AGACATAAAATGGAAATAAAGAAATTACAACAACAAATTGATAAATTAAATCATGCAATC
CCAAAAAAAGATAAAAAAGCCAAATCTGACCTTTTAGAGAAAACTAAAAAGATGGAAGAT
GATTTAAAGAAAAAGCATCANCAAGAATTGGAACAATGTGAAAAACTAAATANAACTACA
CAATCGATTGAAGAATTAAATCTTGAACAACAACAATTACAACAAAAACAACCATCAAAA
GCATACCTTAAAAAAGCACAAAAATTAAAACAAGAAGAAGAAAGAATGAAACAATTAGAA
GAAGATAGAAAAAATCATGTTTCAAAAGGTGTAATTGAAATGAATGATTTCTTAGCAAAA
CTTAAACCATTAAATAAAACTGTTAAATTTATTCCACCAGATGGTGATTGTTTATATCAA
GCAATTACAAACCAATTATTAATTAATAAAGATATTAATGAAGAAGAGAGTAAAAGTTAT
GCAAAGAAATTAAGAACAATAGCATCAGATTATATAAAGAATAATAGNATAATAGTGATG
AGACGATGAGATGATGGGATAATACNAAAAAGTNAAACTAAAGGTAAAAGGTACAAAGAC
A

lnyflkavipaqhkiksapkiviqsrg*cikvicqikvkii*r*fiaaavevffdn*ipa
ftap*atvattpinvi*pqalsdgqtnv*kakeegkqraeetalk*itktkgltdgptfl
tai*etavkkapptpintynslplsspfsvvepsvw*WNKNDGLETTTTPHKLKNAQIAS
IPPHFSLSTTAAKAAVITGLVKLMITA---

---CRLINN**flnttitlidyn*iixwt*n*****w**y*ks*x*r*ngtkt*ngnkei
tttn**ikscnpkkr*ksqi*pfren*kdgr*fkekasxrigtm*ktkxnytid*riks*
tttittkttiksip*kstkiktrrrknetirrr*kkscfkrcn*ne*flskt*tik*nc*
iystrw*lfissnykpiin**ry**rre*klckeiknnsirlyke**xnsdetmr*wdnt
kkxn*r*kvqr

Frame A:
n*iif*kq*fqhniklkvhlkl*snrevdalk*fak*k*klfegns*qrqlkffsiieyq
hlqhpeqr*lqhqsm*ynpkl*vmakqmfekqkkkvnkgqrkqh*nk*pkqrg*qmdqlf
*lqyrkll*rkhhqhqsihiiachclhhfqwwnhrygdgirmmvwrlpqhhinlkmhklp
rfhpisl*vqqlprlq**qdl*n**lq---

---pvd*liiddf*ipllllliitellxgletnddddgdntekvexkgkmvqrhkmeikk
lqqqidklnhaipkkdkkaksdllektkkmeddlkkkhxqeleqceklnxttqsieelnl
eqqqlqqkqpskaylkkaqklkqeeermkqleedrknhvskgviemndflaklkplnktv
kfippdgdclyqaitnqllinkdineeesksyakklrtiasdyiknnxiivmrr*ddgii
xksxtkgkrykd

Frame B:
iklffkssnsstt*n*kct*ncnpierlmh*snlpnksenylkvihssgs*sffr*lnts
iystlsngsyntnqcditpsfk*wpnkclkskrrr*tkgrgnsteinnqnkgvnrwtnfs
ncnigncckesttntnqyi**latvftifsggtigmvme*e*wfgdyhntt*t*kctncl
dstpflfkynscqgcsnnrtcktndys---

---l*id**lmifeyhyysy*l*lnyxldlklmmmmmviilkklkxkvkwykdikwk*rn
ynnklin*imqsqkkikkpnltf*rklkrwkmi*rksixknwnnvkn*ixlhnrlkn*il
nnnnynknnhqkhtlkkhkn*nkkkke*nn*kkiekimfqkv*lk*mis*qnlnh*ikll
nlfhqmvivyikqlqtny*likilmkkrvkvmqrn*eq*hqii*riix****ddemmg*y
xkvklkvkgtkt

Frame C:
lnyflkavipaqhkiksapkiviqsrg*cikvicqikvkii*r*fiaaavevffdn*ipa
ftap*atvattpinvi*pqalsdgqtnv*kakeegkqraeetalk*itktkgltdgptfl
tai*etavkkapptpintynslplsspfsvvepsvw*WNKNDGLETTTTPHKLKNAQIAS
IPPHFSLSTTAAKAAVITGLVKLMITA---

---CRLINN**flnttitlidyn*iixwt*n*****w**y*ks*x*r*ngtkt*ngnkei
tttn**ikscnpkkr*ksqi*pfren*kdgr*fkekasxrigtm*ktkxnytid*riks*
tttittkttiksip*kstkiktrrrknetirrr*kkscfkrcn*ne*flskt*tik*nc*
iystrw*lfissnykpiin**ry**rre*klckeiknnsirlyke**xnsdetmr*wdnt
kkxn*r*kvqr

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHI458 (CHI458Q) /CSM/CH/CHI4-C/CHI458Q.Seq.d/                       1556   0.0
SFD685 (SFD685Q) /CSM/SF/SFD6-D/SFD685Q.Seq.d/                       1152   0.0
AFO180 (AFO180Q) /CSM/AF/AFO1-D/AFO180Q.Seq.d/                       1106   0.0
CHO721 (CHO721Q) /CSM/CH/CHO7-A/CHO721Q.Seq.d/                        991   0.0
AFI213 (AFI213Q) /CSM/AF/AFI2-A/AFI213Q.Seq.d/                        983   0.0
CHB457 (CHB457Q) /CSM/CH/CHB4-C/CHB457Q.Seq.d/                        862   0.0
AHE850 (AHE850Q) /CSM/AH/AHE8-C/AHE850Q.Seq.d/                        720   0.0
CHO805 (CHO805Q) /CSM/CH/CHO8-A/CHO805Q.Seq.d/                        432   e-120
VFK439 (VFK439Q) /CSM/VF/VFK4-B/VFK439Q.Seq.d/                        422   e-117
SSD520 (SSD520Q) /CSM/SS/SSD5-A/SSD520Q.Seq.d/                        422   e-117

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AL929352|AL929352.1 Plasmodium falciparum strain 3D7, chromosome 5, segment 2/4.    48   1e-11  18
AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.    48   9e-10  22
AC117075|AC117075.2 Dictyostelium discoideum chromosome 2 map 5201047-5455781 strain AX4, complete sequence.    44   4e-09  20
BX842643|BX842643.1 Mycoplasma mycoides subsp. mycoides SC genomic DNA, complete  sequence; segment 2/4.    38   5e-09  26
AC116979|AC116979.3 Dictyostelium discoideum chromosome 2 map 6445720-6776760 strain AX4, complete sequence.    36   6e-08  17
AC116956|AC116956.2 Dictyostelium discoideum chromosome 2 map 1418423-1684967 strain AX4, complete sequence.    40   2e-07  20
AP006628|AP006628.1 Onion yellows phytoplasma OY-M DNA, complete genome.    40   2e-07  23
AC117076|AC117076.3 Dictyostelium discoideum chromosome 2 map 3323568-3470138 strain AX4, complete sequence.    38   3e-07  14
BA000026|BA000026.2 Mycoplasma penetrans HF-2 DNA, complete genome.    38   4e-07  30
AL031745|AL031745.8 Plasmodium falciparum DNA from MAL1P2.         36   6e-07  19

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CP000500_661(CP000500|pid:none) Pichia stipitis CBS 6054 chromos...   103   1e-20
CR382135_352(CR382135|pid:none) Debaryomyces hansenii strain CBS...    95   6e-18
CT978603_99(CT978603|pid:none) Synechococcus sp. RCC307 genomic ...    94   1e-17
CP000435_1580(CP000435|pid:none) Synechococcus sp. CC9311, compl...    94   1e-17
CP000095_888(CP000095|pid:none) Prochlorococcus marinus str. NAT...    92   4e-17
CT971583_1072(CT971583|pid:none) Synechococcus WH7803 complete g...    92   5e-17
BX569691_326(BX569691|pid:none) Synechococcus sp. WH8102 complet...    91   1e-16
CR380954_102(CR380954|pid:none) Candida glabrata strain CBS138 c...    90   2e-16
(Q04835) RecName: Full=Uncharacterized membrane protein YMR253C;...    88   8e-16
AY723857_1(AY723857|pid:none) Saccharomyces cerevisiae clone FLH...    86   4e-15

 psg: 0.75  gvh: 0.43  alm: 0.32  top: 0.47  tms: 0.07  mit: 0.18  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 24.0 %: cytoplasmic
	 24.0 %: endoplasmic reticulum
	 20.0 %: nuclear
	 16.0 %: mitochondrial
	  8.0 %: plasma membrane
	  4.0 %: Golgi
	  4.0 %: vesicles of secretory system

>> prediction for CHI458 is cyt

>CHI458F.Seq
AATTAAATTATTTTTTAAAAGCAGTAATTCCAGCACAACATAAAATTAAAAGTGCACCTA
AAATTGTAATCCAATCGAGAGGTTGATGCATTAAAGTAATTTGCCAAATAAAAGTGAAAA
TTATTTGAAGGTAATTCATAGCAGCGGCAGTTGAAGTTTTTTTCGATAATTGAATACCAG
CATTTACAGCACCCTGAGCAACGGTAGCTACAACACCAATCAATGTGATATAACCCCAAG
CTTTAAGTGATGGCCAAACAAATGTTTGAAAAGCAAAAGAAGAAGGTAAACAAAGGGCAG
AGGAAACAGCACTGAAATAAATAACCAAAACAAAGGGGTTAACAGATGGACCAACTTTTC
TAACTGCAATATAGGAAACTGCTGTAAAGAAAGCACCACCAACACCAATCAATACATATA
ATAGCTTGCCACTGTCTTCACCATTTTCAGTGGTGGAACCATCGGTATGGTGATGGAATA
AGAATGATGGTTTGGAGACTACCACAACACCACATAAACTTAAAAATGCACAAATTGCCT
CGATTCCACCCCATTTCTCTTTAAGTACAACAGCTGCCAAGGCTGCAGTAATAACAGGAC
TTGTAAAACTAATGATTACAGCCNNNNNNNNNN

>CHI458Z.Seq
NNNNNNNNNNCCTGTAGATTGATTAATAATTGATGATTTTTGAATACCACTATTACTCTT
ATTGATTATAACTGAATTATTANCTGGACTTGAAACTAATGATGATGATGATGGTGATAA
TACTGAAAAAGTTGAAANTAAAGGTAAAATGGTACAAAGACATAAAATGGAAATAAAGAA
ATTACAACAACAAATTGATAAATTAAATCATGCAATCCCAAAAAAAGATAAAAAAGCCAA
ATCTGACCTTTTAGAGAAAACTAAAAAGATGGAAGATGATTTAAAGAAAAAGCATCANCA
AGAATTGGAACAATGTGAAAAACTAAATANAACTACACAATCGATTGAAGAATTAAATCT
TGAACAACAACAATTACAACAAAAACAACCATCAAAAGCATACCTTAAAAAAGCACAAAA
ATTAAAACAAGAAGAAGAAAGAATGAAACAATTAGAAGAAGATAGAAAAAATCATGTTTC
AAAAGGTGTAATTGAAATGAATGATTTCTTAGCAAAACTTAAACCATTAAATAAAACTGT
TAAATTTATTCCACCAGATGGTGATTGTTTATATCAAGCAATTACAAACCAATTATTAAT
TAATAAAGATATTAATGAAGAAGAGAGTAAAAGTTATGCAAAGAAATTAAGAACAATAGC
ATCAGATTATATAAAGAATAATAGNATAATAGTGATGAGACGATGAGATGATGGGATAAT
ACNAAAAAGTNAAACTAAAGGTAAAAGGTACAAAGACA

>CHI458P.Seq
AATTAAATTATTTTTTAAAAGCAGTAATTCCAGCACAACATAAAATTAAAAGTGCACCTA
AAATTGTAATCCAATCGAGAGGTTGATGCATTAAAGTAATTTGCCAAATAAAAGTGAAAA
TTATTTGAAGGTAATTCATAGCAGCGGCAGTTGAAGTTTTTTTCGATAATTGAATACCAG
CATTTACAGCACCCTGAGCAACGGTAGCTACAACACCAATCAATGTGATATAACCCCAAG
CTTTAAGTGATGGCCAAACAAATGTTTGAAAAGCAAAAGAAGAAGGTAAACAAAGGGCAG
AGGAAACAGCACTGAAATAAATAACCAAAACAAAGGGGTTAACAGATGGACCAACTTTTC
TAACTGCAATATAGGAAACTGCTGTAAAGAAAGCACCACCAACACCAATCAATACATATA
ATAGCTTGCCACTGTCTTCACCATTTTCAGTGGTGGAACCATCGGTATGGTGATGGAATA
AGAATGATGGTTTGGAGACTACCACAACACCACATAAACTTAAAAATGCACAAATTGCCT
CGATTCCACCCCATTTCTCTTTAAGTACAACAGCTGCCAAGGCTGCAGTAATAACAGGAC
TTGTAAAACTAATGATTACAGCC----------CCTGTAGATTGATTAATAATTGATGAT
TTTTGAATACCACTATTACTCTTATTGATTATAACTGAATTATTANCTGGACTTGAAACT
AATGATGATGATGATGGTGATAATACTGAAAAAGTTGAAANTAAAGGTAAAATGGTACAA
AGACATAAAATGGAAATAAAGAAATTACAACAACAAATTGATAAATTAAATCATGCAATC
CCAAAAAAAGATAAAAAAGCCAAATCTGACCTTTTAGAGAAAACTAAAAAGATGGAAGAT
GATTTAAAGAAAAAGCATCANCAAGAATTGGAACAATGTGAAAAACTAAATANAACTACA
CAATCGATTGAAGAATTAAATCTTGAACAACAACAATTACAACAAAAACAACCATCAAAA
GCATACCTTAAAAAAGCACAAAAATTAAAACAAGAAGAAGAAAGAATGAAACAATTAGAA
GAAGATAGAAAAAATCATGTTTCAAAAGGTGTAATTGAAATGAATGATTTCTTAGCAAAA
CTTAAACCATTAAATAAAACTGTTAAATTTATTCCACCAGATGGTGATTGTTTATATCAA
GCAATTACAAACCAATTATTAATTAATAAAGATATTAATGAAGAAGAGAGTAAAAGTTAT
GCAAAGAAATTAAGAACAATAGCATCAGATTATATAAAGAATAATAGNATAATAGTGATG
AGACGATGAGATGATGGGATAATACNAAAAAGTNAAACTAAAGGTAAAAGGTACAAAGAC
A