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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFG203
Library AF
(Link to library)
Clone ID AFG203
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16382-1
Original site URL
Representative seq. ID AFG203E
(Link to Original site)
Representative DNA sequence 
>AFG203 (AFG203Q) /CSM/AF/AFG2-A/AFG203Q.Seq.d/
ATTATTAAACAACTCAAAAGATTTTAATTATATTTTAATATTAAATAAATAAACAAATAA
AAAAAAAAATAAAAAATGGATGGTGAAGATGTTCAAGCTTTAGTTATTGATAATGGTTCA
GGTATGTGTAAAGCTGGTTTTGCTGGTGATGATGCTCCACGTGCTGTTTTCCCATCAATT
GTTGGTCGTCCAAGACACACTGGTGTTATGGTTGGTATGGGTCAAAAGGATTCATATGTC
GGTGATGAAGCTCAATCAAAAAGAGGTATTTTAACACTTAAGTATCCAATTGAACATGGT
ATTGTCACCAATTGGGATGATATGGAAAAAATCTGGCATCATACTTTTTATAATGAACTT
CGTGTTGCTCCAGAGGAACATCCAGTTCTCTTAACTGAAGCTCCATTAAATCCAAAAGCA
AATAGAGAGAAAATGACTCAAATCATGTTTGAAACTTTCAACACTCCAGCAATGTATGTT
GCCATTCAAGCTGTATTATCACTCTATGCCTCTGGTCGTACCACTGGTATCGTTATGGAT
TCAGGTGATGGTGTTTCCCATACTGTACCAATTTACGAAGGTTATGCATTACCACATGCT
ATTTTACGTTTAGATTTAGCTGGTCGTGATCTTACTGATTACATGATGAAAATCTTAACT
GAACGTGGTTACTCATTCACAACAACTGCTGAAAGAGAAATCGTTAGAGATATTAAAGAG
AAATTAGCATACGTTGCATTGGATTTTGAAGCTGAAATGCAAACTGCAGCCTCATCATCT
GCATTAGAGAAATCATATGAATTACCAGATGGTCAAGTTATTACAATTGGTAATGAACGT
TTCCGTTGTCCAGAAGCACTTTTCCAACCATCATTCTTGGGTATGGAGTCTGCTGGTATT
CATGAAACAACCTATAATTCAATCATGAAATGTGATGTTGATATTCGTAAAGATTTATAT
GGTAATGTTGTATTATCAGGTGGTACAACTATGTTCCCAGGTATTGCTGATCGTATGAAT
AAAGAATTAACTGCACTTGCACCATCAACAATGAAAATTAAAATTATTGCACCACCAGAA
CGTAAATACTCTGTATGGATTGGTGGTTCAATTTTAGCTTCACTCTCAACTTTCCAACAA
ATGTGGATTTCAAAAGAAGAATATGAGAATCCGGTCCATCAATTGTTCACAGAAA
sequence update 2001. 6. 9
Translated Amino Acid sequence 
iikqlkrf*lyfnik*ink*kkk*KMDGEDVQALVIDNGSGMCKAGFAGDDAPRAVFPSI
VGRPRHTGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNEL
RVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMD
SGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYMMKILTERGYSFTTTAEREIVRDIKE
KLAYVALDFEAEMQTAASSSALEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESAGI
HETTYNSIMKCDVDIRKDLYGNVVLSGGTTMFPGIADRMNKELTALAPSTMKIKIIAPPE
RKYSVWIGGSILASLSTFQQMWISKEEYENPVHQLFTE
Translated Amino Acid sequence (All Frames) 
Frame A:
iikqlkrf*lyfnik*ink*kkk*KMDGEDVQALVIDNGSGMCKAGFAGDDAPRAVFPSI
VGRPRHTGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNEL
RVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMD
SGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYMMKILTERGYSFTTTAEREIVRDIKE
KLAYVALDFEAEMQTAASSSALEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESAGI
HETTYNSIMKCDVDIRKDLYGNVVLSGGTTMFPGIADRMNKELTALAPSTMKIKIIAPPE
RKYSVWIGGSILASLSTFQQMWISKEEYENPVHQLFTE


Frame B:
llnnskdfnyililnk*tnkkknkkwmvkmfkl*llimvqvcvklvllvmmlhvlfshql
lvvqdtlvlwlvwvkrihmsvmklnqkevf*hlsiqlnmvlspigmiwkksgiilfimnf
vllqrniqfs*lklh*iqkqierk*lksclklstlqqcmlpfklyyhsmplvvplvslwi
qvmvfpilyqftkvmhyhmlfyv*i*lvvillit**ks*lnvvthsqqllkeksleilkr
n*htlhwilklkcklqphhlh*rnhmnyqmvkllqlvmnvsvvqkhfsnhhswvwsllvf
mkqpiiqs*nvmlifvkiymvmlyyqvvqlcsqvlliv*ikn*lhlhhqq*klkllhhqn
vntlyglvvqf*lhsqlsnkcgfqkknmrirsincsqk


Frame C:
y*ttqkiliif*y*inkqikkkikngw*rcssfsy**wfryv*swfcw**cstccfpinc
wsskthwcygwygskgficr**ssikkryfnt*vsn*twychqlg*ygknlasyfl**ts
ccsrgtsssln*ssiksksk*rendsnhv*nfqhssnvcchssciitlclwsyhwyrygf
r*wcfpyctnlrrlcittcyftfrfsws*sy*lhdenln*twllihnnc*krnr*ry*re
isircigf*s*nancsliicireii*itrwssyynw**tfplsrstfptiilgygvcwys
*nnl*fnhem*c*ys*rfiw*cciirwynyvpryc*sye*rinctctinnen*nycttrt
*ilcmdwwfnfsftlnfptnvdfkrri*esgpsivhr
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFG203 (AFG203Q) /CSM/AF/AFG2-A/AFG203Q.Seq.d/                       2218   0.0
AFI479 (AFI479Q) /CSM/AF/AFI4-D/AFI479Q.Seq.d/                       2210   0.0
VFI283 (VFI283Q) /CSM/VF/VFI2-D/VFI283Q.Seq.d/                       2204   0.0
CFC565 (CFC565Q) /CSM/CF/CFC5-C/CFC565Q.Seq.d/                       2190   0.0
AFE187 (AFE187Q) /CSM/AF/AFE1-D/AFE187Q.Seq.d/                       2190   0.0
SFD790 (SFD790Q) /CSM/SF/SFD7-D/SFD790Q.Seq.d/                       2175   0.0
AFO650 (AFO650Q) /CSM/AF/AFO6-C/AFO650Q.Seq.d/                       2167   0.0
SFH143 (SFH143Q) /CSM/SF/SFH1-B/SFH143Q.Seq.d/                       2165   0.0
SFD813 (SFD813Q) /CSM/SF/SFD8-A/SFD813Q.Seq.d/                       2165   0.0
SFL261 (SFL261Q) /CSM/SF/SFL2-C/SFL261Q.Seq.d/                       2161   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

X03283|X03283.1 Dictyostelium discoideum gene for actin A3-S1.  1744  0.0  2
AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.  1685  0.0  2
AC116956|AC116956.2 Dictyostelium discoideum chromosome 2 map 1418423-1684967 strain AX4, complete sequence.  1598  0.0  5
AC115577|AC115577.2 Dictyostelium discoideum chromosome 2 map 4657875-4914984 strain AX4, complete sequence.  1582  0.0  14
X03282|X03282.1 dictyostelium discoideum gene for actin A12.    1550  0.0  2
AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.  1479  0.0  2
X03284|X03284.1 Dictyostelium discoideum gene for actin A3-S2.  1451  0.0  2
M14146|M14146.1 D.discoideum actin 15 gene, complete cds.       1384  0.0  2
AC115579|AC115579.2 Dictyostelium discoideum chromosome 2 map 4915084-5005461 strain AX4, complete sequence.  1384  0.0  2
AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.  1336  0.0  2
dna update 2003. 9. 5
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54H30) RecName: Full=Major actin; AltName: Full=Actin-1; AltNa...   731   0.0
(Q553U6) RecName: Full=Putative actin-22;  &AC116305_3(AC116305|...   727   0.0
ATDO(A93223;A92871;S67999;A03004)actin - slime mold (Dictyosteli...   725   0.0
B23412(B23412)actin 12 - slime mold (Dictyostelium discoideum)        725   0.0
(P02576) RecName: Full=Actin, plasmodial isoform;  &ATFY(A29044;...   724   0.0
X03282_1(X03282|pid:none) dictyostelium discoideum gene for acti...   723   0.0
AY294152_1(AY294152|pid:none) Mayorella sp. JJP-2003 actin mRNA,...   714   0.0
(P07829) RecName: Full=Actin-3; AltName: Full=Actin-3-sub 1;          714   0.0
AY294154_1(AY294154|pid:none) Thecamoeba similis actin mRNA, com...   711   0.0
AY294153_1(AY294153|pid:none) Platyamoeba placida actin mRNA, pa...   710   0.0
protein update 2009. 6.29
PSORT 

 psg: 0.75  gvh: 0.34  alm: 0.42  top: 0.53  tms: 0.00  mit: 0.15  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 44.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: vesicles of secretory system

>> prediction for AFG203 is cyt
5' end seq. ID AFG203F
5' end seq. 
>AFG203F.Seq
ATTATTAAACAACTCAAAAGATTTTAATTATATTTTAATATTAAATAAATAAACAAATAA
AAAAAAAAATAAAAAATGGATGGTGAAGATGTTCAAGCTTTAGTTATTGATAATGGTTCA
GGTATGTGTAAAGCTGGTTTTGCTGGTGATGATGCTCCACGTGCTGTTTTCCCATCAATT
GTTGGTCGTCCAAGACACACTGGTGTTATGGTTGGTATGGGTCAAAAGGATTCATATGTC
GGTGATGAAGCTCAATCAAAAAGAGGTATTTTAACACTTAAGTATCCAATTGAACATGGT
ATTGTCACCAATTGGGATGATATGGAAAAAATCTGGCATCATACTTTTTATAATGAACTT
CGTGTTGCTCCAGAGGAACATCCAGTTCTCTTAACTGAAGCTCCATTAAATCCAAAAGCA
AATAGAGAGAAAATGACTCAAATCATGTTTGAAACTTTCAACACTCCAGCAATGTATGTT
GCCATTCAAGCTGTATTATCACTCTATGCCTCTGGTCGTACCACTGGTATCGTTATGGAT
TCAGGTGATGGTGTTTCCCATACTGTACCAATTTACGAAGGTTATGCATTACCACATGCT
ATTTTACGTTT----------
Length of 5' end seq. 611
3' end seq. ID AFG203Z
3' end seq. 
>AFG203Z.Seq
----------CTTTCAACACTCCAGCAATGTATGTTGCCATTCAAGCTGTATTATCACTC
TATGCCTCTGGTCGTACCACTGGTATCGTTATGGATTCAGGTGATGGTGTTTCCCATACT
GTACCAATTTACGAAGGTTATGCATTACCACATGCTATTTTACGTTTAGATTTAGCTGGT
CGTGATCTTACTGATTACATGATGAAAATCTTAACTGAACGTGGTTACTCATTCACAACA
ACTGCTGAAAGAGAAATCGTTAGAGATATTAAAGAGAAATTAGCATACGTTGCATTGGAT
TTTGAAGCTGAAATGCAAACTGCAGCCTCATCATCTGCATTAGAGAAATCATATGAATTA
CCAGATGGTCAAGTTATTACAATTGGTAATGAACGTTTCCGTTGTCCAGAAGCACTTTTC
CAACCATCATTCTTGGGTATGGAGTCTGCTGGTATTCATGAAACAACCTATAATTCAATC
ATGAAATGTGATGTTGATATTCGTAAAGATTTATATGGTAATGTTGTATTATCAGGTGGT
ACAACTATGTTCCCAGGTATTGCTGATCGTATGAATAAAGAATTAACTGCACTTGCACCA
TCAACAATGAAAATTAAAATTATTGCACCACCAGAACGTAAATACTCTGTATGGATTGGT
GGTTCAATTTTAGCTTCACTCTCAACTTTCCAACAAATGTGGATTTCAAAAGAAGAATAT
GAGAATCCGGTCCATCAATTGTTCACAGAAA
Length of 3' end seq. 741
Connected seq. ID AFG203P
Connected seq. 
>AFG203P.Seq
ATTATTAAACAACTCAAAAGATTTTAATTATATTTTAATATTAAATAAATAAACAAATAA
AAAAAAAAATAAAAAATGGATGGTGAAGATGTTCAAGCTTTAGTTATTGATAATGGTTCA
GGTATGTGTAAAGCTGGTTTTGCTGGTGATGATGCTCCACGTGCTGTTTTCCCATCAATT
GTTGGTCGTCCAAGACACACTGGTGTTATGGTTGGTATGGGTCAAAAGGATTCATATGTC
GGTGATGAAGCTCAATCAAAAAGAGGTATTTTAACACTTAAGTATCCAATTGAACATGGT
ATTGTCACCAATTGGGATGATATGGAAAAAATCTGGCATCATACTTTTTATAATGAACTT
CGTGTTGCTCCAGAGGAACATCCAGTTCTCTTAACTGAAGCTCCATTAAATCCAAAAGCA
AATAGAGAGAAAATGACTCAAATCATGTTTGAAACTTTCAACACTCCAGCAATGTATGTT
GCCATTCAAGCTGTATTATCACTCTATGCCTCTGGTCGTACCACTGGTATCGTTATGGAT
TCAGGTGATGGTGTTTCCCATACTGTACCAATTTACGAAGGTTATGCATTACCACATGCT
ATTTTACGTTT----------CTTTCAACACTCCAGCAATGTATGTTGCCATTCAAGCTG
TATTATCACTCTATGCCTCTGGTCGTACCACTGGTATCGTTATGGATTCAGGTGATGGTG
TTTCCCATACTGTACCAATTTACGAAGGTTATGCATTACCACATGCTATTTTACGTTTAG
ATTTAGCTGGTCGTGATCTTACTGATTACATGATGAAAATCTTAACTGAACGTGGTTACT
CATTCACAACAACTGCTGAAAGAGAAATCGTTAGAGATATTAAAGAGAAATTAGCATACG
TTGCATTGGATTTTGAAGCTGAAATGCAAACTGCAGCCTCATCATCTGCATTAGAGAAAT
CATATGAATTACCAGATGGTCAAGTTATTACAATTGGTAATGAACGTTTCCGTTGTCCAG
AAGCACTTTTCCAACCATCATTCTTGGGTATGGAGTCTGCTGGTATTCATGAAACAACCT
ATAATTCAATCATGAAATGTGATGTTGATATTCGTAAAGATTTATATGGTAATGTTGTAT
TATCAGGTGGTACAACTATGTTCCCAGGTATTGCTGATCGTATGAATAAAGAATTAACTG
CACTTGCACCATCAACAATGAAAATTAAAATTATTGCACCACCAGAACGTAAATACTCTG
TATGGATTGGTGGTTCAATTTTAGCTTCACTCTCAACTTTCCAACAAATGTGGATTTCAA
AAGAAGAATATGAGAATCCGGTCCATCAATTGTTCACAGAAA
Length of connected seq. 1352
Full length Seq ID AFG203E
Full length Seq. 
>AFG203E.Seq
ATTATTAAACAACTCAAAAGATTTTAATTATATTTTAATATTAAATAAATAAACAAATAA
AAAAAAAAATAAAAAATGGATGGTGAAGATGTTCAAGCTTTAGTTATTGATAATGGTTCA
GGTATGTGTAAAGCTGGTTTTGCTGGTGATGATGCTCCACGTGCTGTTTTCCCATCAATT
GTTGGTCGTCCAAGACACACTGGTGTTATGGTTGGTATGGGTCAAAAGGATTCATATGTC
GGTGATGAAGCTCAATCAAAAAGAGGTATTTTAACACTTAAGTATCCAATTGAACATGGT
ATTGTCACCAATTGGGATGATATGGAAAAAATCTGGCATCATACTTTTTATAATGAACTT
CGTGTTGCTCCAGAGGAACATCCAGTTCTCTTAACTGAAGCTCCATTAAATCCAAAAGCA
AATAGAGAGAAAATGACTCAAATCATGTTTGAAACTTTCAACACTCCAGCAATGTATGTT
GCCATTCAAGCTGTATTATCACTCTATGCCTCTGGTCGTACCACTGGTATCGTTATGGAT
TCAGGTGATGGTGTTTCCCATACTGTACCAATTTACGAAGGTTATGCATTACCACATGCT
ATTTTACGTTTAGATTTAGCTGGTCGTGATCTTACTGATTACATGATGAAAATCTTAACT
GAACGTGGTTACTCATTCACAACAACTGCTGAAAGAGAAATCGTTAGAGATATTAAAGAG
AAATTAGCATACGTTGCATTGGATTTTGAAGCTGAAATGCAAACTGCAGCCTCATCATCT
GCATTAGAGAAATCATATGAATTACCAGATGGTCAAGTTATTACAATTGGTAATGAACGT
TTCCGTTGTCCAGAAGCACTTTTCCAACCATCATTCTTGGGTATGGAGTCTGCTGGTATT
CATGAAACAACCTATAATTCAATCATGAAATGTGATGTTGATATTCGTAAAGATTTATAT
GGTAATGTTGTATTATCAGGTGGTACAACTATGTTCCCAGGTATTGCTGATCGTATGAAT
AAAGAATTAACTGCACTTGCACCATCAACAATGAAAATTAAAATTATTGCACCACCAGAA
CGTAAATACTCTGTATGGATTGGTGGTTCAATTTTAGCTTCACTCTCAACTTTCCAACAA
ATGTGGATTTCAAAAGAAGAATATGAGAATCCGGTCCATCAATTGTTCACAGAAA
Length of full length seq. 1195