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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFG169
Library AF
(Link to library)
Clone ID AFG169
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16382-1
Original site URL
Representative seq. ID AFG169P
(Link to Original site)
Representative DNA sequence 
>AFG169 (AFG169Q) /CSM/AF/AFG1-C/AFG169Q.Seq.d/
ATAGTTTTAATAAACAAATATTTAAATATAATACAACAATAAAATGGACGGTGAAGATGT
TCAAGCTTTAGTTATCGATAATGGTTCTGGTATGTGTAAAGCCGGTTTTGCTGGTGATGA
TGCCCCACGTGCTGTTTTCCCATCAATTGTTGGTCGTCCAAGACACACTGGTGTTATGGT
CGGTATGGGTCAAAAAGACTCTTACGTAGGTGATGAAGCCCAATCAAAGAGAGGTATCTT
AACCCTCAAATACCCAATTGAACACGGTATTGTTACCAACTGGGATGATATGGAAAAAAT
CTGGCATCATACTTTCTACAATGAACTCCGTGTTGCTCCAGAAGAACACCCAGTTCTCTT
AACTGAAGCCCCATTAAATCCAAAGGCCAACAGAGAAAAAATGACCCAAATTATGTTTGA
AACCTTCAACACCCCAGCCATGTACGTTGCCATTCAAGCTGTCTTATCCTTATATGCCTC
TGGTCGTACCACCGGTATTGTTATGGATTCAGGTGATGGTGTCTCCCACACTGTTCCAAT
CTATGAAGGTTATGCCTTACCACATGCCATCTTACGTTTAGATTTAGCTGGTCGTGATCT
TACTGATTACCTGATGAAAATCTTAAXXXXXXXXXXACCTGATGAAAAATCTTAACTGAA
CGTGGTTACTCATTCACCACCACTGCTGAAAGAGAAATCGTCAGAGATATCAAAGAAAAA
TTAGCCTACGTCGCCCTCGACTTTGAAGCTGAAATGCAAACTGCTGCCTCATCATCAGCC
CTCGAAAAATCATACGAATTACCAGACGGTCAAGTCATCACCATCGGTAATGAACGTTTC
CGTTGCCCAGAAGCCCTCTTCCAACCATCACTCTTAGGTATGGAATCAGCTGGTATCCAC
GAAACCACCTATAACTCCATCATGAAATGTGATGTTGATATCCGTAAAGATTTATACGGC
AATGTCGTCTTATCTGGTGGTACAACTATGTTCCCAGGTATTGCTGATCGTATGAACAAA
GAATTAACTGCTTTAGCCCCATCAACCATGAAAATTAAAATCATTGCTCCACCAGAACGT
AAATACTCTGTCTGGATTGGTGGATCTATCTTAGCTTCACTCTCAACTTTCCAACAAATG
TGGATCTCCAAAGAAGAATATGACGAATCAGGTCCATCAATTGTCCACAGAAAATGTTTC
TAAACAATTAAAACTAGTGATGAAAGTGCTTCTCACACAAACAAATATATTTAAATGTAT
AATAATAATAAAATAAATATTTTTA
sequence update 2001. 6. 2
Translated Amino Acid sequence 
*f**tni*i*YNNKMDGEDVQALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHTGVMV
GMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLL
TEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVSHTVPI
YEGYALPHAILRLDLAGRDLTDYLMKIL---

---LMKNLN*twllihhhc*krnrqryqrkislrrprl*s*nanccliisprkiiritrr
sshhhr**tfplprsplptitlrygiswyprnhl*lhhem*c*yp*rfirqcrliwwyny
vpryc*syeqrincfspinhen*nhcstrt*ilcldwwiylsftlnfptnvdlqrri*ri
rsincpqkmflnn*n***kcfshkqiylnv****nkyf
Translated Amino Acid sequence (All Frames) 
Frame A:
ivlinkylniiqq*ngr*rcssfsyr*wfwyv*srfcw**cptccfpincwsskthwcyg
rygskrllrr**spikerylnpqipn*trycyqlg*ygknlasyflq*tpccsrrtpssl
n*spikskgqqrkndpnyv*nlqhpshvrchsscliliclwsyhrycygfr*wclphcsn
l*rlclttchltfrfsws*sy*lpdenl---

---t**kiltergysftttaereivrdikeklayvaldfeaemqtaasssaleksyelpd
gqvitignerfrcpealfqpsllgmesagihettynsimkcdvdirkdlygnvvlsggtt
mfpgiadrmnkeltalapstmkikiiapperkysvwiggsilaslstfqqmwiskeeyde
sgpsivhrkcf*tiktsdesashtnkyi*mynnnkinif

Frame B:
*f**tni*i*YNNKMDGEDVQALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHTGVMV
GMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLL
TEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVSHTVPI
YEGYALPHAILRLDLAGRDLTDYLMKIL---

---pdeks*lnvvthsppllkeksseiskkn*ptspstlklkckllphhqpsknhtnyqt
vksspsvmnvsvaqkpssnhhs*vwnqlvstkppitps*nvmlisvkiytamssylvvql
csqvlliv*tkn*ll*phqp*klksllhqnvntlsglvdls*lhsqlsnkcgspkknmtn
qvhqlstenvskqlklvmkvlltqtnifkciiiik*ifl

Frame C:
sfnkqifkynttikwtvkmfkl*lsimvlvcvkpvllvmmphvlfshqllvvqdtlvlws
vwvkktlt*vmkpnqrevs*psntqlntvllptgmiwkksgiilstmnsvllqkntqfs*
lkph*iqrptekk*pklclkpstpqpctlpfklsypymplvvppvllwiqvmvsptlfqs
mkvmpyhmpsyv*i*lvvillit**ks*---

---LMKNLN*twllihhhc*krnrqryqrkislrrprl*s*nanccliisprkiiritrr
sshhhr**tfplprsplptitlrygiswyprnhl*lhhem*c*yp*rfirqcrliwwyny
vpryc*syeqrincfspinhen*nhcstrt*ilcldwwiylsftlnfptnvdlqrri*ri
rsincpqkmflnn*n***kcfshkqiylnv****nkyf
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFG169 (AFG169Q) /CSM/AF/AFG1-C/AFG169Q.Seq.d/                       2401   0.0
VFM863 (VFM863Q) /CSM/VF/VFM8-C/VFM863Q.Seq.d/                       1233   0.0
VFI269 (VFI269Q) /CSM/VF/VFI2-C/VFI269Q.Seq.d/                       1233   0.0
VFE460 (VFE460Q) /CSM/VF/VFE4-C/VFE460Q.Seq.d/                       1233   0.0
VFE112 (VFE112Q) /CSM/VF/VFE1-A/VFE112Q.Seq.d/                       1233   0.0
VFC818 (VFC818Q) /CSM/VF/VFC8-A/VFC818Q.Seq.d/                       1233   0.0
AFH563 (AFH563Q) /CSM/AF/AFH5-C/AFH563Q.Seq.d/                       1233   0.0
AFG608 (AFG608Q) /CSM/AF/AFG6-A/AFG608Q.Seq.d/                       1233   0.0
VFM283 (VFM283Q) /CSM/VF/VFM2-D/VFM283Q.Seq.d/                       1229   0.0
VFK573 (VFK573Q) /CSM/VF/VFK5-D/VFK573Q.Seq.d/                       1229   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.  1233   0.0    2
X03281|X03281.1 Dictyostelium discoideum gene for actin A8.      1068   0.0    1
AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.   999   0.0    11
AC116963|AC116963.2 Dictyostelium discoideum chromosome 2 map 4657875-4914984 strain AX4, complete sequence.   884   0.0    11
AC116956|AC116956.2 Dictyostelium discoideum chromosome 2 map 1418423-1684967 strain AX4, complete sequence.   882   0.0    9
M14146|M14146.1 D.discoideum actin 15 gene, complete cds.        1019   0.0    2
AC115680|AC115680.3 Dictyostelium discoideum chromosome 2 map 4915084-5005461 strain AX4, complete sequence.  1019   0.0    2
U25660|U25660.1 Dictyostelium discoideum actin gene, partial cds.   987   0.0    1
X03283|X03283.1 Dictyostelium discoideum gene for actin A3-S1.    583   0.0    2
AF508263|AF508263.1 Pyrocystis lunula actin mRNA, complete cds.   589   0.0    3
dna update 2004. 5. 6
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54H30) RecName: Full=Major actin; AltName: Full=Actin-1; AltNa...   397   0.0
(Q553U6) RecName: Full=Putative actin-22;  &AC116305_3(AC116305|...   397   0.0
(P02576) RecName: Full=Actin, plasmodial isoform;  &ATFY(A29044;...   396   0.0
ATDO(A93223;A92871;S67999;A03004)actin - slime mold (Dictyosteli...   395   0.0
B23412(B23412)actin 12 - slime mold (Dictyostelium discoideum)        390   0.0
X03282_1(X03282|pid:none) dictyostelium discoideum gene for acti...   389   0.0
(P07829) RecName: Full=Actin-3; AltName: Full=Actin-3-sub 1;          387   0.0
AY294154_1(AY294154|pid:none) Thecamoeba similis actin mRNA, com...   389   0.0
(P02578) RecName: Full=Actin-1;  &ATAX(A92886;A03004) &V00002_1(...   388   0.0
AF082863_1(AF082863|pid:none) Dreissena polymorpha cytoplasmic a...   387   0.0
protein update 2009. 4.20
PSORT 

 psg: 0.75  gvh: 0.34  alm: 0.42  top: 0.53  tms: 0.00  mit: 0.15  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 36.0 %: nuclear
	 20.0 %: cytoskeletal
	  4.0 %: peroxisomal

>> prediction for AFG169 is cyt
5' end seq. ID AFG169F
5' end seq. 
>AFG169F.Seq
ATAGTTTTAATAAACAAATATTTAAATATAATACAACAATAAAATGGACGGTGAAGATGT
TCAAGCTTTAGTTATCGATAATGGTTCTGGTATGTGTAAAGCCGGTTTTGCTGGTGATGA
TGCCCCACGTGCTGTTTTCCCATCAATTGTTGGTCGTCCAAGACACACTGGTGTTATGGT
CGGTATGGGTCAAAAAGACTCTTACGTAGGTGATGAAGCCCAATCAAAGAGAGGTATCTT
AACCCTCAAATACCCAATTGAACACGGTATTGTTACCAACTGGGATGATATGGAAAAAAT
CTGGCATCATACTTTCTACAATGAACTCCGTGTTGCTCCAGAAGAACACCCAGTTCTCTT
AACTGAAGCCCCATTAAATCCAAAGGCCAACAGAGAAAAAATGACCCAAATTATGTTTGA
AACCTTCAACACCCCAGCCATGTACGTTGCCATTCAAGCTGTCTTATCCTTATATGCCTC
TGGTCGTACCACCGGTATTGTTATGGATTCAGGTGATGGTGTCTCCCACACTGTTCCAAT
CTATGAAGGTTATGCCTTACCACATGCCATCTTACGTTTAGATTTAGCTGGTCGTGATCT
TACTGATTACCTGATGAAAATCTTAA----------
Length of 5' end seq. 626
3' end seq. ID AFG169Z
3' end seq. 
>AFG169Z.Seq
----------ACCTGATGAAAAATCTTAACTGAACGTGGTTACTCATTCACCACCACTGC
TGAAAGAGAAATCGTCAGAGATATCAAAGAAAAATTAGCCTACGTCGCCCTCGACTTTGA
AGCTGAAATGCAAACTGCTGCCTCATCATCAGCCCTCGAAAAATCATACGAATTACCAGA
CGGTCAAGTCATCACCATCGGTAATGAACGTTTCCGTTGCCCAGAAGCCCTCTTCCAACC
ATCACTCTTAGGTATGGAATCAGCTGGTATCCACGAAACCACCTATAACTCCATCATGAA
ATGTGATGTTGATATCCGTAAAGATTTATACGGCAATGTCGTCTTATCTGGTGGTACAAC
TATGTTCCCAGGTATTGCTGATCGTATGAACAAAGAATTAACTGCTTTAGCCCCATCAAC
CATGAAAATTAAAATCATTGCTCCACCAGAACGTAAATACTCTGTCTGGATTGGTGGATC
TATCTTAGCTTCACTCTCAACTTTCCAACAAATGTGGATCTCCAAAGAAGAATATGACGA
ATCAGGTCCATCAATTGTCCACAGAAAATGTTTCTAAACAATTAAAACTAGTGATGAAAG
TGCTTCTCACACAAACAAATATATTTAAATGTATAATAATAATAAAATAAATATTTTTA
Length of 3' end seq. 649
Connected seq. ID AFG169P
Connected seq. 
>AFG169P.Seq
ATAGTTTTAATAAACAAATATTTAAATATAATACAACAATAAAATGGACGGTGAAGATGT
TCAAGCTTTAGTTATCGATAATGGTTCTGGTATGTGTAAAGCCGGTTTTGCTGGTGATGA
TGCCCCACGTGCTGTTTTCCCATCAATTGTTGGTCGTCCAAGACACACTGGTGTTATGGT
CGGTATGGGTCAAAAAGACTCTTACGTAGGTGATGAAGCCCAATCAAAGAGAGGTATCTT
AACCCTCAAATACCCAATTGAACACGGTATTGTTACCAACTGGGATGATATGGAAAAAAT
CTGGCATCATACTTTCTACAATGAACTCCGTGTTGCTCCAGAAGAACACCCAGTTCTCTT
AACTGAAGCCCCATTAAATCCAAAGGCCAACAGAGAAAAAATGACCCAAATTATGTTTGA
AACCTTCAACACCCCAGCCATGTACGTTGCCATTCAAGCTGTCTTATCCTTATATGCCTC
TGGTCGTACCACCGGTATTGTTATGGATTCAGGTGATGGTGTCTCCCACACTGTTCCAAT
CTATGAAGGTTATGCCTTACCACATGCCATCTTACGTTTAGATTTAGCTGGTCGTGATCT
TACTGATTACCTGATGAAAATCTTAA----------ACCTGATGAAAAATCTTAACTGAA
CGTGGTTACTCATTCACCACCACTGCTGAAAGAGAAATCGTCAGAGATATCAAAGAAAAA
TTAGCCTACGTCGCCCTCGACTTTGAAGCTGAAATGCAAACTGCTGCCTCATCATCAGCC
CTCGAAAAATCATACGAATTACCAGACGGTCAAGTCATCACCATCGGTAATGAACGTTTC
CGTTGCCCAGAAGCCCTCTTCCAACCATCACTCTTAGGTATGGAATCAGCTGGTATCCAC
GAAACCACCTATAACTCCATCATGAAATGTGATGTTGATATCCGTAAAGATTTATACGGC
AATGTCGTCTTATCTGGTGGTACAACTATGTTCCCAGGTATTGCTGATCGTATGAACAAA
GAATTAACTGCTTTAGCCCCATCAACCATGAAAATTAAAATCATTGCTCCACCAGAACGT
AAATACTCTGTCTGGATTGGTGGATCTATCTTAGCTTCACTCTCAACTTTCCAACAAATG
TGGATCTCCAAAGAAGAATATGACGAATCAGGTCCATCAATTGTCCACAGAAAATGTTTC
TAAACAATTAAAACTAGTGATGAAAGTGCTTCTCACACAAACAAATATATTTAAATGTAT
AATAATAATAAAATAAATATTTTTA
Length of connected seq. 1275
Full length Seq ID -
Full length Seq. -
Length of full length seq. -