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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFG168
Library AF
(Link to library)
Clone ID AFG168
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16252-1
Original site URL
Representative seq. ID AFG168P
(Link to Original site)
Representative DNA sequence 
>AFG168 (AFG168Q) /CSM/AF/AFG1-C/AFG168Q.Seq.d/
ACTTATTACTTTATTTTTTTTTTATCTCTATCTATATATATATCATTTTTTAAAANGAGC
GTCATCAAATCAATTAAAGCCAGAGAAATTTTAGATTCACGTGGTAACCCAACTGTTGAA
GTTGACCTTTACACCGAAAAAGATGGNGTTGTTTCATCATTCAGAGCTGCAGTTCCATCA
GGNGCATCAACTGGTATTTATGAAGCTGTTGAATTAAGAGATGGNGATAAATCAAGATAC
TTAGGTAAAGGNGTACTCAAAGCAATCAAAAACATTCTCGAAGTCATTCAACCAGCCGTC
ATTGGTAAATCAGTTTCCGATCAAGCTGCTATCGATAAATTAATGATCGATCTCGATGGT
NCCCCAAATAAAGGTAAATTAGGNGCCAACGCTATCCTTGCAGTCAGTTTAGCTGTTTGC
AGAGCTGGTGCTGCTGATAGAAATTTACCACTTTACAAATATATCTCANAAATTGCAGGT
ACTAAAATGAGATTACCAGTTCCAGCATTCAATGTTATCAXXXXXXXXXXACACAGGTTT
AGTTAAAATTGGTATGGATTGTGCTGCCTCTGAATTCAAAGTTGAAAATGGTTACGATCT
TGACTTCAAAACTAAAAACAATGACGGTTCAGCTGTTATCTCTGGTGAAAAATTAGGTGA
TCTCTACAGAGAATTCATTAAAGAATATCCAATCATTTCAATTGAAGATCCATTTGATCA
AGATGATTGGGAATCATACACTAAATTAACAGCATCAGTTGATATTCAAATTGTTGGTGA
TGATTTACTTGTTACCAACCCAGAAAGAATTAAGACTGGTATTGAAAAGAAAGCATGTAA
TGCTCTTTTATTAAAAGTTAACCAAATTGGTTCAGTCACTGAAGCAATTCGTGCCGCTCT
TGACTCAAAGAATGCATCATGGGGTGTCATGGTTAGCCATCGTTCAGGTGAAACTGAAGA
TACCTTCATTGCTGATTTAGTTGTTGGTTTAGGTACTGGTCAAATTAAAACTGGTGCTCC
ATGTAGATCAGAAAGATTAGCCAAATACAATCAACTTGTTAGAATTAACGAAGAGTTAGG
TGAAAACCATAACTATGCGGTTTAACTTTCCGTAAATTTTAATTTTACTCATAATA
sequence update 2001. 6. 2
Translated Amino Acid sequence 
TYYFIFFLSLSIYISFFKXSVIKSIKAREILDSRGNPTVEVDLYTEKDGVVSSFRAAVPS
GASTGIYEAVELRDGDKSRYLGKGVLKAIKNILEVIQPAVIGKSVSDQAAIDKLMIDLDG
XPNKGKLGANAILAVSLAVCRAGAADRNLPLYKYISXIAGTKMRLPVPAFNVI---

---TGLVKIGMDCAASEFKVENGYDLDFKTKNNDGSAVISGEKLGDLYREFIKEYPIISI
EDPFDQDDWESYTKLTASVDIQIVGDDLLVTNPERIKTGIEKKACNALLLKVNQIGSVTE
AIRAALDSKNASWGVMVSHRSGETEDTFIADLVVGLGTGQIKTGAPCRSERLAKYNQLVR
INEELGENHNYAV*lsvnfnfthn
Translated Amino Acid sequence (All Frames) 
Frame A:
TYYFIFFLSLSIYISFFKXSVIKSIKAREILDSRGNPTVEVDLYTEKDGVVSSFRAAVPS
GASTGIYEAVELRDGDKSRYLGKGVLKAIKNILEVIQPAVIGKSVSDQAAIDKLMIDLDG
XPNKGKLGANAILAVSLAVCRAGAADRNLPLYKYISXIAGTKMRLPVPAFNVI---

---tqv*lklvwivlplnsklkmvtiltsklktmtvqllslvkn*vistenslkniqsfq
lkihlikmignhtln*qhqlifkllvmiyllptqkelrlvlkrkhvmlfy*kltklvqsl
kqfvplltqrmhhgvswlaivqvklkipslli*llv*vlvklklvlhvdqkd*pntinll
eltks*vktitmrfnfp*ilillii

Frame B:
litlfffylylyiyhflkxassnqlkpekf*ihvvtqllkltftpkkmxlfhhselqfhq
xhqlvfmklln*emxinqdt*vkxyskqsktfsksfnqpslvnqfpikllsin**sismv
pqikvn*xptlslqsv*lfaelvllieiyhftnisxklqvlk*dyqfqhsmls---

---hrfs*nwyglccl*iqs*kwlrs*lqn*kq*rfscylw*kir*slqrih*risnhfn
*rsi*sr*lgiih*insis*ysncw**ftcyqprkn*dwy*kesm*csfiks*pnwfsh*
snscrs*lkecimgchg*psfr*n*rylhc*fscwfrywsn*nwcsm*irkisqiqstc*
n*rrvr*kp*lcgltfrkf*fys*

Frame C:
lllyfffisiyiyiif*xerhqin*sqrnfrftw*pnc*s*plhrkrwxcfiiqscssir
xinwyl*sc*ikrwx*ikilr*rxtqsnqkhsrshstsrhw*isfrsscyr*indrsrwx
pk*r*irxqrypcsqfsclqswcc**kfttlqiylxncry*neitsssiqcy---

---TGLVKIGMDCAASEFKVENGYDLDFKTKNNDGSAVISGEKLGDLYREFIKEYPIISI
EDPFDQDDWESYTKLTASVDIQIVGDDLLVTNPERIKTGIEKKACNALLLKVNQIGSVTE
AIRAALDSKNASWGVMVSHRSGETEDTFIADLVVGLGTGQIKTGAPCRSERLAKYNQLVR
INEELGENHNYAV*lsvnfnfthn
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFG168 (AFG168Q) /CSM/AF/AFG1-C/AFG168Q.Seq.d/                       2099   0.0
SFH339 (SFH339Q) /CSM/SF/SFH3-B/SFH339Q.Seq.d/                       1193   0.0
AFN685 (AFN685Q) /CSM/AF/AFN6-D/AFN685Q.Seq.d/                       1193   0.0
VFL491 (VFL491Q) /CSM/VF/VFL4-D/VFL491Q.Seq.d/                       1179   0.0
VFE233 (VFE233Q) /CSM/VF/VFE2-B/VFE233Q.Seq.d/                       1179   0.0
SSI448 (SSI448Q) /CSM/SS/SSI4-B/SSI448Q.Seq.d/                       1179   0.0
SLA770 (SLA770Q) /CSM/SL/SLA7-C/SLA770Q.Seq.d/                       1179   0.0
SFE744 (SFE744Q) /CSM/SF/SFE7-B/SFE744Q.Seq.d/                       1179   0.0
SFC432 (SFC432Q) /CSM/SF/SFC4-B/SFC432Q.Seq.d/                       1179   0.0
SFC169 (SFC169Q) /CSM/SF/SFC1-C/SFC169Q.Seq.d/                       1179   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

X80474|X80474.1 N.frontalis enolase gene.                          64   3e-56  9
BD183626|BD183626.1 Material for diagnosing malaria and immune antigen for suppressing growth of plasmodium.    76   7e-46  8
BD183625|BD183625.1 Material for diagnosing malaria and immune antigen for suppressing growth of plasmodium.    76   7e-46  8
AB026051|AB026051.1 Plasmodium falciparum mRNA for enolase, complete cds.    76   7e-46  8
U00152|U00152.1 Plasmodium falciparum enolase gene complete cds.    76   4e-44  7
Y17298|Y17298.1 Cunninghamella elegans mRNA for enolase.           60   3e-41  8
AY522934|AY522934.1 Artemia sp. SBH266677 2-phospho-D-glycerate hydrolase mRNA, partial cds.    70   6e-40  6
BM395755|BM395755.1 5009-0-10-H08.t.1 Chilcoat/Turkewitz cDNA (large fraction) Tetrahymena thermophila cDNA, mRNA sequence.   121   1e-39  4
BI504105|BI504105.1 BB170030B20E01 Bee Brain Normalized/Subtracted Library, BB17 Apis mellifera cDNA clone BB170030B20E01 5', mRNA sequence.    88   1e-37  4
BM169441|BM169441.1 EST571964 PyBS Plasmodium yoelii yoelii cDNA clone PYCPQ90 5' end, mRNA sequence.    92   8e-36  4
dna update 2004. 5. 6
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Z68318_5(Z68318|pid:none) Caenorhabditis elegans Cosmid T21B10, ...   263   e-113
(Q54RK5) RecName: Full=Enolase A;          EC=4.2.1.11; AltName:...   377   e-103
BC009018_1(BC009018|pid:none) Mus musculus enolase 2, gamma neur...   257   3e-99
EF137905_1(EF137905|pid:none) Helianthus annuus plastid enolase ...   285   3e-75
AF100985_1(AF100985|pid:none) Penaeus monodon phosphopyruvate hy...   280   6e-74
AC016662_10(AC016662|pid:none) Arabidopsis thaliana chromosome 1...   278   2e-73
EU964132_1(EU964132|pid:none) Zea mays clone 276124 enolase mRNA...   277   4e-73
AK243111_1(AK243111|pid:none) Oryza sativa Japonica Group cDNA, ...   273   6e-72
(P56252) RecName: Full=Enolase;          EC=4.2.1.11; AltName: F...   272   2e-71
T12341(T12341) phosphopyruvate hydratase (EC 4.2.1.11) - common ...   272   2e-71
protein update 2009. 4.20
PSORT 

 psg: 0.97  gvh: 0.47  alm: 0.34  top: 0.60  tms: 0.00  mit: 0.35  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 24.0 %: nuclear
	 20.0 %: mitochondrial
	  4.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for AFG168 is cyt
5' end seq. ID AFG168F
5' end seq. 
>AFG168F.Seq
ACTTATTACTTTATTTTTTTTTTATCTCTATCTATATATATATCATTTTTTAAAANGAGC
GTCATCAAATCAATTAAAGCCAGAGAAATTTTAGATTCACGTGGTAACCCAACTGTTGAA
GTTGACCTTTACACCGAAAAAGATGGNGTTGTTTCATCATTCAGAGCTGCAGTTCCATCA
GGNGCATCAACTGGTATTTATGAAGCTGTTGAATTAAGAGATGGNGATAAATCAAGATAC
TTAGGTAAAGGNGTACTCAAAGCAATCAAAAACATTCTCGAAGTCATTCAACCAGCCGTC
ATTGGTAAATCAGTTTCCGATCAAGCTGCTATCGATAAATTAATGATCGATCTCGATGGT
NCCCCAAATAAAGGTAAATTAGGNGCCAACGCTATCCTTGCAGTCAGTTTAGCTGTTTGC
AGAGCTGGTGCTGCTGATAGAAATTTACCACTTTACAAATATATCTCANAAATTGCAGGT
ACTAAAATGAGATTACCAGTTCCAGCATTCAATGTTATCA----------
Length of 5' end seq. 520
3' end seq. ID AFG168Z
3' end seq. 
>AFG168Z.Seq
----------ACACAGGTTTAGTTAAAATTGGTATGGATTGTGCTGCCTCTGAATTCAAA
GTTGAAAATGGTTACGATCTTGACTTCAAAACTAAAAACAATGACGGTTCAGCTGTTATC
TCTGGTGAAAAATTAGGTGATCTCTACAGAGAATTCATTAAAGAATATCCAATCATTTCA
ATTGAAGATCCATTTGATCAAGATGATTGGGAATCATACACTAAATTAACAGCATCAGTT
GATATTCAAATTGTTGGTGATGATTTACTTGTTACCAACCCAGAAAGAATTAAGACTGGT
ATTGAAAAGAAAGCATGTAATGCTCTTTTATTAAAAGTTAACCAAATTGGTTCAGTCACT
GAAGCAATTCGTGCCGCTCTTGACTCAAAGAATGCATCATGGGGTGTCATGGTTAGCCAT
CGTTCAGGTGAAACTGAAGATACCTTCATTGCTGATTTAGTTGTTGGTTTAGGTACTGGT
CAAATTAAAACTGGTGCTCCATGTAGATCAGAAAGATTAGCCAAATACAATCAACTTGTT
AGAATTAACGAAGAGTTAGGTGAAAACCATAACTATGCGGTTTAACTTTCCGTAAATTTT
AATTTTACTCATAATA
Length of 3' end seq. 606
Connected seq. ID AFG168P
Connected seq. 
>AFG168P.Seq
ACTTATTACTTTATTTTTTTTTTATCTCTATCTATATATATATCATTTTTTAAAANGAGC
GTCATCAAATCAATTAAAGCCAGAGAAATTTTAGATTCACGTGGTAACCCAACTGTTGAA
GTTGACCTTTACACCGAAAAAGATGGNGTTGTTTCATCATTCAGAGCTGCAGTTCCATCA
GGNGCATCAACTGGTATTTATGAAGCTGTTGAATTAAGAGATGGNGATAAATCAAGATAC
TTAGGTAAAGGNGTACTCAAAGCAATCAAAAACATTCTCGAAGTCATTCAACCAGCCGTC
ATTGGTAAATCAGTTTCCGATCAAGCTGCTATCGATAAATTAATGATCGATCTCGATGGT
NCCCCAAATAAAGGTAAATTAGGNGCCAACGCTATCCTTGCAGTCAGTTTAGCTGTTTGC
AGAGCTGGTGCTGCTGATAGAAATTTACCACTTTACAAATATATCTCANAAATTGCAGGT
ACTAAAATGAGATTACCAGTTCCAGCATTCAATGTTATCA----------ACACAGGTTT
AGTTAAAATTGGTATGGATTGTGCTGCCTCTGAATTCAAAGTTGAAAATGGTTACGATCT
TGACTTCAAAACTAAAAACAATGACGGTTCAGCTGTTATCTCTGGTGAAAAATTAGGTGA
TCTCTACAGAGAATTCATTAAAGAATATCCAATCATTTCAATTGAAGATCCATTTGATCA
AGATGATTGGGAATCATACACTAAATTAACAGCATCAGTTGATATTCAAATTGTTGGTGA
TGATTTACTTGTTACCAACCCAGAAAGAATTAAGACTGGTATTGAAAAGAAAGCATGTAA
TGCTCTTTTATTAAAAGTTAACCAAATTGGTTCAGTCACTGAAGCAATTCGTGCCGCTCT
TGACTCAAAGAATGCATCATGGGGTGTCATGGTTAGCCATCGTTCAGGTGAAACTGAAGA
TACCTTCATTGCTGATTTAGTTGTTGGTTTAGGTACTGGTCAAATTAAAACTGGTGCTCC
ATGTAGATCAGAAAGATTAGCCAAATACAATCAACTTGTTAGAATTAACGAAGAGTTAGG
TGAAAACCATAACTATGCGGTTTAACTTTCCGTAAATTTTAATTTTACTCATAATA
Length of connected seq. 1126
Full length Seq ID -
Full length Seq. -
Length of full length seq. -