>CHH715 (CHH715Q) /CSM/CH/CHH7-A/CHH715Q.Seq.d/
ATATCAATAATAAATATTATTACATATACAAAACAAAATTAATAAAACTTAAACTTTATT
TTTCCCCTACATTGGCCAAACTTTAATTTGCCTTTGTACAATTATTCTCTTTAAACTCTT
ATTATTAAAATTCATTTTAAAAACTCTCATTTCTAAAAAAAACTAAAACAAAAAAAAAAN
GGTCAATTTTACAATAGATCAAATTCGTTCAATTATGGATCGTCGNGAAAATATCAAAAA
TATGTCTGTTATTGCTCATGTNGATCATGGTAAAACTACTCTTACTGATTCATTAATTCA
AAGAGCTGGTATTATTGCANATAAGGTATCTGGTAATGCAGGATATATGTCATGTCGTCC
ANATGAACAATTACGNGGTATTACCATAAAGTCATCATCTGTTTCACTTCACTTTGAAAN
GCCAAAAGXXXXXXXXXXTCGTCAGACTTAACTGATGATATTGATAATGGTTTGGTGGCT
TTTAATCAAGATGTTAAAAGTCGTGCCAATTATCTCTATGATAAACATTCTTGGGATAAG
AATGATGCAGTTAACATTTGGTCATTTGGTCCAGAATATAAGGGTCCAAATGTTTTAGTT
AACAAAACCAAAGGGTGTTCAATATCTTCATGAAATTAAAGACTCTATGGTTTCAGCATT
CCAATGGATCTCAAATGAAGGTGTATTATGTGGTGAGAAAATGAGATCGATTCGTTTCAA
CGTTTATGATGTAATGCTTCATGCCGATGCAATTCATAGAGGTAGTGGTCAATTTATTCC
AACAACTCGTCGTGTAATGTATGCCTCCCAACTATCAGCTCAACCAACACTTTTGGAACC
AATGTATTTGGTTGATATTAGTACTCCTGAAAGTGGTATTAGTGGTATCTACTCTGTACT
CAATCGTAGGAGAGGTGTTGTCATTGGTGAAGAACGTCGTATTGGTACTCCATTATTCAA
TATCAAAGCTTATCTTCCTGTAATGGAATCATTTGGTCTCACTGCTGATTTACGTTCAAG
TACTGCTGGTCAAGCTTTCCCTCAATGTGTTTTTGATCATTGGAGTTCAATTGGTATCGT
TGGTCAAGATAAAAGATCAACTGAAGTAGCTTCATCAATTAGAAAGAGAAAAGGTTTAAA
TCC

yq**illhiqnkinkt*tlffpyigqtliclctiilfkllllkfilktliskkn*NKKKX
VNFTIDQIRSIMDRRENIKNMSVIAHVDHGKTTLTDSLIQRAGIIAXKVSGNAGYMSCRP
XEQLRGITIKSSSVSLHFEXPK---

---SSDLTDDIDNGLVAFNQDVKSRANYLYDKHSWDKNDAVNIWSFGPEYKGPNVLVNKT
KGCSISS*n*rlygfsipmdlk*rcimw*eneidsfqrl*cnascrcns*r*wsiysnns
scnvclptisstntfgtnvfg*y*ys*kwy*wyllctqs*ercchw*rtsywysiiqyqs
lsscngiiwshc*ftfkycwssfpsmcf*slefnwyrwsr*kin*ssfin*kekrfks

Frame A:
isiiniitytkqn**nlnfifplhwpnfnlplynysl*tliikihfknshf*kklkqkkx
gqfynrsnsfnygssxkyqkyvcycscxsw*nysy*finskswyycx*giw*criyvmss
x*titxyyhkviicftsl*xak---

---SSDLTDDIDNGLVAFNQDVKSRANYLYDKHSWDKNDAVNIWSFGPEYKGPNVLVNKT
KGCSISS*n*rlygfsipmdlk*rcimw*eneidsfqrl*cnascrcns*r*wsiysnns
scnvclptisstntfgtnvfg*y*ys*kwy*wyllctqs*ercchw*rtsywysiiqyqs
lsscngiiwshc*ftfkycwssfpsmcf*slefnwyrwsr*kin*ssfin*kekrfks

Frame B:
yq**illhiqnkinkt*tlffpyigqtliclctiilfkllllkfilktliskkn*NKKKX
VNFTIDQIRSIMDRRENIKNMSVIAHVDHGKTTLTDSLIQRAGIIAXKVSGNAGYMSCRP
XEQLRGITIKSSSVSLHFEXPK---

---rqt*lmilimvwwllikmlkvvpiisminilgirmmqltfghlvqnirvqmf*ltkp
kgvqylheikdsmvsafqwisnegvlcgekmrsirfnvydvmlhadaihrgsgqfipttr
rvmyasqlsaqptllepmylvdistpesgisgiysvlnrrrgvvigeerrigtplfnika
ylpvmesfgltadlrsstagqafpqcvfdhwssigivgqdkrstevassirkrkgln

Frame C:
innkyyyiyktkliklklyfsptlakl*fafvqlfslnsyy*nsf*klsflkktktkkkx
silq*ikfvqlwivxkiskicllllmximvkllllih*fkelvllxirylvmqdichvvx
mnnyxvlp*shhlfhftlkxqk---

---vrln**y**wfggf*src*kscqlsl**tflg*e*cs*hlviwsri*gskcfs*qnq
rvfnifmklktlwfqhsngsqmkvyyvvrk*drfvstfmm*cfmpmqfievvvnlfqqlv
v*cmppnyqlnqhfwnqciwlilvllkvvlvvstlysivgevlslvknvvlvlhysiskl
ifl*wnhlvslliyvqvllvklslnvfliigvqlvslvkikdqlk*lhqlerekv*i

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHH715 (CHH715Q) /CSM/CH/CHH7-A/CHH715Q.Seq.d/                       1814   0.0
AHB695 (AHB695Q) /CSM/AH/AHB6-D/AHB695Q.Seq.d/                       1780   0.0
SHF216 (SHF216Q) /CSM/SH/SHF2-A/SHF216Q.Seq.d/                       1390   0.0
AHM516 (AHM516Q) /CSM/AH/AHM5-A/AHM516Q.Seq.d/                       1382   0.0
AHL348 (AHL348Q) /CSM/AH/AHL3-B/AHL348Q.Seq.d/                       1382   0.0
AHE765 (AHE765Q) /CSM/AH/AHE7-C/AHE765Q.Seq.d/                       1382   0.0
AHD146 (AHD146Q) /CSM/AH/AHD1-B/AHD146Q.Seq.d/                       1382   0.0
AHC654 (AHC654Q) /CSM/AH/AHC6-C/AHC654Q.Seq.d/                       1382   0.0
AHB739 (AHB739Q) /CSM/AH/AHB7-B/AHB739Q.Seq.d/                       1382   0.0
AHB514 (AHB514Q) /CSM/AH/AHB5-A/AHB514Q.Seq.d/                       1382   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M26017|M26017.1 D.discoideum elongation factor 2 mRNA, complete cds.   145   4e-87  9
X15386|X15386.1 Dictyostelium discoideum H6 gene mRNA.            228   3e-60  2
BI863585|BI863585.1 kx46a06.y1 Parastrongyloides trichosuri FL pAMP1 v1 Chiapelli McCarter Parastrongyloides trichosuri cDNA 5' similar to SW:EF2_CAEEL P29691 ELONGATION FACTOR 2 ;, mRNA sequence.    66   4e-18  4
BI863575|BI863575.1 kx45h07.y1 Parastrongyloides trichosuri FL pAMP1 v1 Chiapelli McCarter Parastrongyloides trichosuri cDNA 5' similar to SW:EF2_CHICK Q90705 ELONGATION FACTOR 2 ;, mRNA sequence.    66   8e-15  3
BI451383|BI451383.1 kx23e09.y1 Parastrongyloides trichosuri FL pAMP1 v1 Chiapelli McCarter Parastrongyloides trichosuri cDNA 5' similar to SW:EF2_CAEEL P29691 ELONGATION FACTOR 2 ;, mRNA sequence.    66   8e-15  3
BM056853|BM056853.1 2154-85 hindgut and Malpighian tubule subtracted cDNA library Ctenocephalides felis cDNA clone 2154-85, mRNA sequence.    92   2e-14  1
BM058379|BM058379.1 2191-29 hindgut and Malpighian tubule subtracted cDNA library Ctenocephalides felis cDNA clone 2191-29, mRNA sequence.    90   1e-13  1
BM058578|BM058578.1 2193-94 hindgut and Malpighian tubule subtracted cDNA library Ctenocephalides felis cDNA clone 2193-94, mRNA sequence.    90   1e-13  1
X92797|X92797.1 E.histolytica partial mRNA; expressed sequence tag (clone HMZP0097).    46   1e-12  4
AZ931993|AZ931993.1 474.dhz93a01.s1 Saccharomyces unisporus NRRL Y-1556 Saccharomyces unisporus genomic clone 474.dhz93a01.s1, DNA sequence.    84   6e-12  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY069933_1(AY069933|pid:none) Rana sylvatica eukaryotic translat...   214   8e-68
BC089730_1(BC089730|pid:none) Xenopus tropicalis MGC108369 prote...   213   6e-67
AY391422_1(AY391422|pid:none) Danio rerio eukaryotic translation...   212   1e-66
AK032997_1(AK032997|pid:none) Mus musculus 12 days embryo male w...   213   1e-66
(P05197) RecName: Full=Elongation factor 2;          Short=EF-2;...   213   1e-66
AK159514_1(AK159514|pid:none) Mus musculus osteoclast-like cell ...   213   1e-66
BC060707_1(BC060707|pid:none) Mus musculus eukaryotic translatio...   213   1e-66
AK161993_1(AK161993|pid:none) Mus musculus 12 days embryo male w...   213   1e-66
AJ278147_1(AJ278147|pid:none) Rattus norvegicus partial Ef-2 gen...   213   1e-66
BC063919_1(BC063919|pid:none) Xenopus tropicalis eukaryotic tran...   211   2e-66

 psg: 0.47  gvh: 0.43  alm: 0.50  top: 0.53  tms: 0.00  mit: 0.25  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 36.0 %: nuclear
	  8.0 %: mitochondrial
	  8.0 %: vesicles of secretory system
	  4.0 %: vacuolar

>> prediction for CHH715 is cyt

>CHH715F.Seq
ATATCAATAATAAATATTATTACATATACAAAACAAAATTAATAAAACTTAAACTTTATT
TTTCCCCTACATTGGCCAAACTTTAATTTGCCTTTGTACAATTATTCTCTTTAAACTCTT
ATTATTAAAATTCATTTTAAAAACTCTCATTTCTAAAAAAAACTAAAACAAAAAAAAAAN
GGTCAATTTTACAATAGATCAAATTCGTTCAATTATGGATCGTCGNGAAAATATCAAAAA
TATGTCTGTTATTGCTCATGTNGATCATGGTAAAACTACTCTTACTGATTCATTAATTCA
AAGAGCTGGTATTATTGCANATAAGGTATCTGGTAATGCAGGATATATGTCATGTCGTCC
ANATGAACAATTACGNGGTATTACCATAAAGTCATCATCTGTTTCACTTCACTTTGAAAN
GCCAAAAGNNNNNNNNNN

>CHH715Z.Seq
NNNNNNNNNNTCGTCAGACTTAACTGATGATATTGATAATGGTTTGGTGGCTTTTAATCA
AGATGTTAAAAGTCGTGCCAATTATCTCTATGATAAACATTCTTGGGATAAGAATGATGC
AGTTAACATTTGGTCATTTGGTCCAGAATATAAGGGTCCAAATGTTTTAGTTAACAAAAC
CAAAGGGTGTTCAATATCTTCATGAAATTAAAGACTCTATGGTTTCAGCATTCCAATGGA
TCTCAAATGAAGGTGTATTATGTGGTGAGAAAATGAGATCGATTCGTTTCAACGTTTATG
ATGTAATGCTTCATGCCGATGCAATTCATAGAGGTAGTGGTCAATTTATTCCAACAACTC
GTCGTGTAATGTATGCCTCCCAACTATCAGCTCAACCAACACTTTTGGAACCAATGTATT
TGGTTGATATTAGTACTCCTGAAAGTGGTATTAGTGGTATCTACTCTGTACTCAATCGTA
GGAGAGGTGTTGTCATTGGTGAAGAACGTCGTATTGGTACTCCATTATTCAATATCAAAG
CTTATCTTCCTGTAATGGAATCATTTGGTCTCACTGCTGATTTACGTTCAAGTACTGCTG
GTCAAGCTTTCCCTCAATGTGTTTTTGATCATTGGAGTTCAATTGGTATCGTTGGTCAAG
ATAAAAGATCAACTGAAGTAGCTTCATCAATTAGAAAGAGAAAAGGTTTAAATCC

>CHH715P.Seq
ATATCAATAATAAATATTATTACATATACAAAACAAAATTAATAAAACTTAAACTTTATT
TTTCCCCTACATTGGCCAAACTTTAATTTGCCTTTGTACAATTATTCTCTTTAAACTCTT
ATTATTAAAATTCATTTTAAAAACTCTCATTTCTAAAAAAAACTAAAACAAAAAAAAAAN
GGTCAATTTTACAATAGATCAAATTCGTTCAATTATGGATCGTCGNGAAAATATCAAAAA
TATGTCTGTTATTGCTCATGTNGATCATGGTAAAACTACTCTTACTGATTCATTAATTCA
AAGAGCTGGTATTATTGCANATAAGGTATCTGGTAATGCAGGATATATGTCATGTCGTCC
ANATGAACAATTACGNGGTATTACCATAAAGTCATCATCTGTTTCACTTCACTTTGAAAN
GCCAAAAG----------TCGTCAGACTTAACTGATGATATTGATAATGGTTTGGTGGCT
TTTAATCAAGATGTTAAAAGTCGTGCCAATTATCTCTATGATAAACATTCTTGGGATAAG
AATGATGCAGTTAACATTTGGTCATTTGGTCCAGAATATAAGGGTCCAAATGTTTTAGTT
AACAAAACCAAAGGGTGTTCAATATCTTCATGAAATTAAAGACTCTATGGTTTCAGCATT
CCAATGGATCTCAAATGAAGGTGTATTATGTGGTGAGAAAATGAGATCGATTCGTTTCAA
CGTTTATGATGTAATGCTTCATGCCGATGCAATTCATAGAGGTAGTGGTCAATTTATTCC
AACAACTCGTCGTGTAATGTATGCCTCCCAACTATCAGCTCAACCAACACTTTTGGAACC
AATGTATTTGGTTGATATTAGTACTCCTGAAAGTGGTATTAGTGGTATCTACTCTGTACT
CAATCGTAGGAGAGGTGTTGTCATTGGTGAAGAACGTCGTATTGGTACTCCATTATTCAA
TATCAAAGCTTATCTTCCTGTAATGGAATCATTTGGTCTCACTGCTGATTTACGTTCAAG
TACTGCTGGTCAAGCTTTCCCTCAATGTGTTTTTGATCATTGGAGTTCAATTGGTATCGT
TGGTCAAGATAAAAGATCAACTGAAGTAGCTTCATCAATTAGAAAGAGAAAAGGTTTAAA
TCC