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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CHH533
Library CH
(Link to library)
Clone ID CHH533
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U11302-1
Original site URL
Representative seq. ID CHH533P
(Link to Original site)
Representative DNA sequence 
>CHH533 (CHH533Q) /CSM/CH/CHH5-B/CHH533Q.Seq.d/
AAACTACAAATTTGGATGTTAAATTAATTATAATTTGTTAATATAACATTTTGTATATAT
ATATATATAAAAAATAAAAAACTCAAATGACAGGATTTTCAATTCCTAATATGAGTATGT
TACTCAAATTAGCAAATAGTAGATTGGTATTACATAAAAATAAAAATTTTGAACAAAGTA
ATGTTCATAAAAAAGAGATTGCAGAATTATTACAATTAGGTAAAGAAGAACAAGCAAGAG
TTAGAACGGTTTCAGTTATTAATGAAGATTATCACACTGAAGTTTTAGGTATTTTAATTA
TTTATTGTGAAACTTTAGCAAATAGAATTAGAGGAATTGAAGGTGTAAAGGTATGCCCAC
CAGATTTAAAAGAAGCATGTTGTAGTATTATATTTGCATCACCATATTTAGATAAACAAG
TTGAATTATATAAGATTAGAAAGAGATTAATTGAAAAATTTGGTAAAAAATTCCCAGAGG
AATGTATAGATTGTTGTTGTATCAATCCAAAGATTGTACATAGATTATCAAATAAACCAC
CTGAAGAXXXXXXXXXXGTTTGAATATTTCAATACTCNTGCCAGTTTATATAGCAATTCA
AGCCGTTNTNTCTTTGTATGCATCTGGTCGTACCACTGGTACNGTATTGGATTGTGGTGA
TGGTGTTTGTCATACCGTGCCAATCTATGAAGGTTATTCTATTCCACATGCAATTAAAAG
AATCGATATCGGTGGTCGTGATATCACAGAGTATTTAATGCGTTTACTCACTGAAAGAGG
TTATGCTTTCACTACAACCGCCGAAAGAGAAATCGTACGTGATATCAAAGAGAAAACTTC
ATTTGTCTCTCAAGATTTCTATGCCTCTATGAATGCTCCAGAGGATAATAATATACTCAA
GGATTATACGATGCCCGATAAACAAGTTTTAACCATTGGTAAAGAAAGATTCCGTTGTTA
TGAAGCTTTCTTTGACCNTTCAGTANTAGGTAAAGATCAAAATGGTATTCATCACNTCTT
AAATGAAACTATCACNTCTTGTGATATTGATATTCGTCGTGATCTCTATAAAAATATTGT
AGTTTCTGGTGGTTCAACAATGGCAACAGGTTTTGAAAAACGTTTACAAAAAGAAATGGA
ACTTTTAGTTTCAAATCNTCAAGTCCAAATTAAAATTAATGCACCACCAAATCGTGATAT
TAGTGTTTGGTGTGGTGGTTCGGTTTTAGGTGATCTTAAAACTTTTTCGGATCAATGGAT
TACAAAACAAGAATATGATGAAGTTGGTAGAGGAATTGTTCATAGAAAATGTTTTTAAAT
AAATAAAATAATAAATAATATTTTAAA
sequence update 2002.10.25
Translated Amino Acid sequence 
ttnldvkliiic*ynilyiyiykk*KTQMTGFSIPNMSMLLKLANSRLVLHKNKNFEQSN
VHKKEIAELLQLGKEEQARVRTVSVINEDYHTEVLGILIIYCETLANRIRGIEGVKVCPP
DLKEACCSIIFASPYLDKQVELYKIRKRLIEKFGKKFPEECIDCCCINPKIVHRLSNKPP
E---

---LNISILXPVYIAIQAVXSLYASGRTTGTVLDCGDGVCHTVPIYEGYSIPHAIKRIDI
GGRDITEYLMRLLTERGYAFTTTAEREIVRDIKEKTSFVSQDFYASMNAPEDNNILKDYT
MPDKQVLTIGKERFRCYEAFFDXSVXGKDQNGIHHXLNETITSCDIDIRRDLYKNIVVSG
GSTMATGFEKRLQKEMELLVSNXQVQIKINAPPNRDISVWCGGSVLGDLKTFSDQWITKQ
EYDEVGRGIVHRKCF*inkiinnil
Translated Amino Acid sequence (All Frames) 
Frame A:
klqiwmln*l*fvnitfciyiyiknkklk*qdfqfli*vcysn*qivdwyyikikilnkv
mfikkrlqnyyn*vkknkqelerfqllmkiitlkf*vf*lfivkl*qieleelkv*ryah
qi*kkhvvvlylhhhi*inklnyirlerd*lknlvknsqrnv*ivvvsiqrlyidyqinh
lk---

---v*ifqyscqfi*qfkpxxlcmhlvvplvxywivvmvfvipcqsmkvilfhmqlkesi
svvvisqsi*cvyslkevmlslqppkeksyviskrklhlslkismpl*mlqriiiysrii
rcpinkf*plvkkdsvvmklsltxqx*vkikmvfits*mklsxlvilifvvisikil*fl
vvqqwqqvlknvykkkwnf*fqixksklklmhhqivilvfgvvvrf*vilklfringlqn
knmmklveelfienvfk*ik**iif*

Frame B:
nykfgc*inynlli*hfvyiyi*kiknsndrifns*yeyvtqisk**igit*k*kf*tk*
cs*krdcriitir*rrtsks*ngfsy**rlsh*sfryfnyll*nfsk*n*rn*rckgmpt
rfkrsml*yyicitifr*ts*ii*d*kein*kiw*kiprgmyrlllyqskdct*iik*tt
*r---

---feyfntxaslysnssrxxfvciwsyhwyxiglw*wclsyranl*rlfystcn*knry
rws*yhrvfnafth*krlcfhynrrkrnrt*yqrenficlsrflclyecsrg**ytqgly
dar*tsfnhw*rkipll*sfl*pfsxr*rskwyssxlk*nyhxl*y*yss*sl*kycsfw
wfnngnrf*ktftkrngtfsfkssspn*n*cttks*y*clvwwfgfr*s*nffgsmdykt
ri**sw*rncs*kmflnk*nnk*yfk

Frame C:
ttnldvkliiic*ynilyiyiykk*KTQMTGFSIPNMSMLLKLANSRLVLHKNKNFEQSN
VHKKEIAELLQLGKEEQARVRTVSVINEDYHTEVLGILIIYCETLANRIRGIEGVKVCPP
DLKEACCSIIFASPYLDKQVELYKIRKRLIEKFGKKFPEECIDCCCINPKIVHRLSNKPP
E---

---LNISILXPVYIAIQAVXSLYASGRTTGTVLDCGDGVCHTVPIYEGYSIPHAIKRIDI
GGRDITEYLMRLLTERGYAFTTTAEREIVRDIKEKTSFVSQDFYASMNAPEDNNILKDYT
MPDKQVLTIGKERFRCYEAFFDXSVXGKDQNGIHHXLNETITSCDIDIRRDLYKNIVVSG
GSTMATGFEKRLQKEMELLVSNXQVQIKINAPPNRDISVWCGGSVLGDLKTFSDQWITKQ
EYDEVGRGIVHRKCF*inkiinnil
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHH533 (CHH533Q) /CSM/CH/CHH5-B/CHH533Q.Seq.d/                       2333   0.0
CHF491 (CHF491Q) /CSM/CH/CHF4-D/CHF491Q.Seq.d/                       1435   0.0
AHM227 (AHM227Q) /CSM/AH/AHM2-B/AHM227Q.Seq.d/                       1433   0.0
VHB304 (VHB304Q) /CSM/VH/VHB3-A/VHB304Q.Seq.d/                       1409   0.0
AHL385 (AHL385Q) /CSM/AH/AHL3-D/AHL385Q.Seq.d/                       1407   0.0
AHF512 (AHF512Q) /CSM/AH/AHF5-A/AHF512Q.Seq.d/                       1407   0.0
VHC732 (VHC732Q) /CSM/VH/VHC7-B/VHC732Q.Seq.d/                       1403   0.0
VHA813 (VHA813Q) /CSM/VH/VHA8-A/VHA813Q.Seq.d/                       1374   0.0
VHD820 (VHD820Q) /CSM/VH/VHD8-A/VHD820Q.Seq.d/                       1362   0.0
SHC311 (SHC311Q) /CSM/SH/SHC3-A/SHC311Q.Seq.d/                       1350   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AF234970|AF234970.1 Argonauta nodosa actin gene, partial cds.      98   2e-25  4
AF382346|AF382346.1 Cryptosporidium baileyi isolate 764 actin gene, partial cds.    90   9e-21  2
BP189654|BP189654.1 Dugesia japonica cDNA, clone: 06735_HH, expressed in the head.   103   2e-20  2
AJ269586|AJ269586.1 Artemia franciscana mRNA for actin (actin 302 gene), clone RT-PCR-14.    66   2e-20  3
AJ269585|AJ269585.1 Artemia franciscana mRNA for actin (actin 302 gene), clone RT-PCR-7.    66   2e-20  3
AJ269584|AJ269584.1 Artemia franciscana mRNA for actin (actin 302 gene), clone RT-PCR-6.    66   2e-20  3
AJ269583|AJ269583.1 Artemia franciscana mRNA for actin (actin 302 gene), clone RT-PCR-3.    66   2e-20  3
AJ269581|AJ269581.1 Artemia franciscana mRNA for actin (actin 302 gene), clone RT-PCR-18.    66   2e-20  3
AJ269580|AJ269580.1 Artemia franciscana mRNA for actin (actin 302 gene), clone RT-PCR-13.    66   2e-20  3
AJ269579|AJ269579.1 Artemia franciscana mRNA for actin (actin 302 gene), clone RT-PCR-9.    66   2e-20  3
dna update 2004.11.22
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AM411663_1(AM411663|pid:none) Plasmodiophora brassicae actII gen...   307   6e-82
(P18600) RecName: Full=Actin, clone 205;  &S11450(S11450) &X5260...   306   8e-82
(P53458) RecName: Full=Actin-5; Flags: Fragment;  &U27836_1(U278...   306   8e-82
(P45886) RecName: Full=Actin-3, muscle-specific;  &L12255_1(L122...   306   1e-81
AY294156_1(AY294156|pid:none) Pelomyxa palustris actin mRNA, com...   306   1e-81
(P53470) RecName: Full=Actin-1;  &M80334_1(M80334|pid:none)           305   2e-81
(P53459) RecName: Full=Actin-6; Flags: Fragment;  &U27837_1(U278...   305   2e-81
(O16808) RecName: Full=Actin;  &AF017427_1(AF017427|pid:none)         305   2e-81
(P10981) RecName: Full=Actin-87E;  &AE014297_1743(AE014297|pid:n...   305   3e-81
AY113405_1(AY113405|pid:none) Drosophila melanogaster RE12057 fu...   305   3e-81
protein update 2009. 4. 2
PSORT 

 psg: 0.87  gvh: 0.48  alm: 0.34  top: 0.70  tms: 0.00  mit: 0.33  mip: 0.05
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: mitochondrial
	 36.0 %: cytoplasmic
	 16.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: peroxisomal

>> prediction for CHH533 is mit
5' end seq. ID CHH533F
5' end seq. 
>CHH533F.Seq
AAACTACAAATTTGGATGTTAAATTAATTATAATTTGTTAATATAACATTTTGTATATAT
ATATATATAAAAAATAAAAAACTCAAATGACAGGATTTTCAATTCCTAATATGAGTATGT
TACTCAAATTAGCAAATAGTAGATTGGTATTACATAAAAATAAAAATTTTGAACAAAGTA
ATGTTCATAAAAAAGAGATTGCAGAATTATTACAATTAGGTAAAGAAGAACAAGCAAGAG
TTAGAACGGTTTCAGTTATTAATGAAGATTATCACACTGAAGTTTTAGGTATTTTAATTA
TTTATTGTGAAACTTTAGCAAATAGAATTAGAGGAATTGAAGGTGTAAAGGTATGCCCAC
CAGATTTAAAAGAAGCATGTTGTAGTATTATATTTGCATCACCATATTTAGATAAACAAG
TTGAATTATATAAGATTAGAAAGAGATTAATTGAAAAATTTGGTAAAAAATTCCCAGAGG
AATGTATAGATTGTTGTTGTATCAATCCAAAGATTGTACATAGATTATCAAATAAACCAC
CTGAAGANNNNNNNNNN
Length of 5' end seq. 557
3' end seq. ID CHH533Z
3' end seq. 
>CHH533Z.Seq
NNNNNNNNNNGTTTGAATATTTCAATACTCNTGCCAGTTTATATAGCAATTCAAGCCGTT
NTNTCTTTGTATGCATCTGGTCGTACCACTGGTACNGTATTGGATTGTGGTGATGGTGTT
TGTCATACCGTGCCAATCTATGAAGGTTATTCTATTCCACATGCAATTAAAAGAATCGAT
ATCGGTGGTCGTGATATCACAGAGTATTTAATGCGTTTACTCACTGAAAGAGGTTATGCT
TTCACTACAACCGCCGAAAGAGAAATCGTACGTGATATCAAAGAGAAAACTTCATTTGTC
TCTCAAGATTTCTATGCCTCTATGAATGCTCCAGAGGATAATAATATACTCAAGGATTAT
ACGATGCCCGATAAACAAGTTTTAACCATTGGTAAAGAAAGATTCCGTTGTTATGAAGCT
TTCTTTGACCNTTCAGTANTAGGTAAAGATCAAAATGGTATTCATCACNTCTTAAATGAA
ACTATCACNTCTTGTGATATTGATATTCGTCGTGATCTCTATAAAAATATTGTAGTTTCT
GGTGGTTCAACAATGGCAACAGGTTTTGAAAAACGTTTACAAAAAGAAATGGAACTTTTA
GTTTCAAATCNTCAAGTCCAAATTAAAATTAATGCACCACCAAATCGTGATATTAGTGTT
TGGTGTGGTGGTTCGGTTTTAGGTGATCTTAAAACTTTTTCGGATCAATGGATTACAAAA
CAAGAATATGATGAAGTTGGTAGAGGAATTGTTCATAGAAAATGTTTTTAAATAAATAAA
ATAATAAATAATATTTTAAA
Length of 3' end seq. 800
Connected seq. ID CHH533P
Connected seq. 
>CHH533P.Seq
AAACTACAAATTTGGATGTTAAATTAATTATAATTTGTTAATATAACATTTTGTATATAT
ATATATATAAAAAATAAAAAACTCAAATGACAGGATTTTCAATTCCTAATATGAGTATGT
TACTCAAATTAGCAAATAGTAGATTGGTATTACATAAAAATAAAAATTTTGAACAAAGTA
ATGTTCATAAAAAAGAGATTGCAGAATTATTACAATTAGGTAAAGAAGAACAAGCAAGAG
TTAGAACGGTTTCAGTTATTAATGAAGATTATCACACTGAAGTTTTAGGTATTTTAATTA
TTTATTGTGAAACTTTAGCAAATAGAATTAGAGGAATTGAAGGTGTAAAGGTATGCCCAC
CAGATTTAAAAGAAGCATGTTGTAGTATTATATTTGCATCACCATATTTAGATAAACAAG
TTGAATTATATAAGATTAGAAAGAGATTAATTGAAAAATTTGGTAAAAAATTCCCAGAGG
AATGTATAGATTGTTGTTGTATCAATCCAAAGATTGTACATAGATTATCAAATAAACCAC
CTGAAGA----------GTTTGAATATTTCAATACTCNTGCCAGTTTATATAGCAATTCA
AGCCGTTNTNTCTTTGTATGCATCTGGTCGTACCACTGGTACNGTATTGGATTGTGGTGA
TGGTGTTTGTCATACCGTGCCAATCTATGAAGGTTATTCTATTCCACATGCAATTAAAAG
AATCGATATCGGTGGTCGTGATATCACAGAGTATTTAATGCGTTTACTCACTGAAAGAGG
TTATGCTTTCACTACAACCGCCGAAAGAGAAATCGTACGTGATATCAAAGAGAAAACTTC
ATTTGTCTCTCAAGATTTCTATGCCTCTATGAATGCTCCAGAGGATAATAATATACTCAA
GGATTATACGATGCCCGATAAACAAGTTTTAACCATTGGTAAAGAAAGATTCCGTTGTTA
TGAAGCTTTCTTTGACCNTTCAGTANTAGGTAAAGATCAAAATGGTATTCATCACNTCTT
AAATGAAACTATCACNTCTTGTGATATTGATATTCGTCGTGATCTCTATAAAAATATTGT
AGTTTCTGGTGGTTCAACAATGGCAACAGGTTTTGAAAAACGTTTACAAAAAGAAATGGA
ACTTTTAGTTTCAAATCNTCAAGTCCAAATTAAAATTAATGCACCACCAAATCGTGATAT
TAGTGTTTGGTGTGGTGGTTCGGTTTTAGGTGATCTTAAAACTTTTTCGGATCAATGGAT
TACAAAACAAGAATATGATGAAGTTGGTAGAGGAATTGTTCATAGAAAATGTTTTTAAAT
AAATAAAATAATAAATAATATTTTAAA
Length of connected seq. 1337
Full length Seq ID -
Full length Seq. -
Length of full length seq. -