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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFA618
Library AF
(Link to library)
Clone ID AFA618
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16381-1
Original site URL
Representative seq. ID AFA618P
(Link to Original site)
Representative DNA sequence 
>AFA618 (AFA618Q) /CSM/AF/AFA6-A/AFA618Q.Seq.d/
ATTGTAGTATTTTGTTCATATTCAAATTAAATATCAATACAATTAAAAAAAGTTAAAATT
AAATTTGTAAAATCAATTTGTAACAAAAACTAGTAATTTAAAAAAAAAAAAACCCAAAAA
AAAAAXXXXXXXXXXTCCANATTTCCATANATGTGTTNAAANACGTGGNGGNATNTTAAG
NTGNGAATTTNATCCNCCAANACAACCNAGATCCCTTGATTGGGCTGAAAATGAAAATGA
TGATCGTGACTATGATGATCGNGNCTATGATGATGATGAANATGATGGTGATTATNATGG
CCGNGACGGTGACTATGATGGNGATTATGATGGNGATTATGATGATGNCNATTATT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
l*yfvhiqikyqyn*kklklnl*NQFVTKTSNLKKKKPKKK---

---PXFHXCVXXRGGXLXXEFXPPXQPRSLDWAENENDDRDYDDRXYDDDEXDGDYXGRD
GDYDGDYDGDYDDXXY
Translated Amino Acid sequence (All Frames) 
Frame A:
ivvfcsysn*isiqlkkvkikfvksicnkn**fkkkktqkk---

---sxfpxmcxxtwxxxkxxixsxxttxip*lg*k*k**s*l**sxl****x*w*lxwpx
r*l*wxl*wxl**xxl

Frame B:
l*yfvhiqikyqyn*kklklnl*NQFVTKTSNLKKKKPKKK---

---PXFHXCVXXRGGXLXXEFXPPXQPRSLDWAENENDDRDYDDRXYDDDEXDGDYXGRD
GDYDGDYDGDYDDXXY

Frame C:
csilfifklnintikks*n*ickinl*qklvi*kkknpkkk---

---xisixvxkxvxxx*xxnxixqxnxdpliglkmkmmivtmmixxmmmmxmmvixmaxt
vtmmximmximmmxii
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SLF502 (SLF502Q) /CSM/SL/SLF5-A/SLF502Q.Seq.d/                        155   5e-37
SLF326 (SLF326Q) /CSM/SL/SLF3-B/SLF326Q.Seq.d/                        155   5e-37
SHF559 (SHF559Q) /CSM/SH/SHF5-C/SHF559Q.Seq.d/                        155   5e-37
SHA894 (SHA894Q) /CSM/SH/SHA8-D/SHA894Q.Seq.d/                        155   5e-37
SHA483 (SHA483Q) /CSM/SH/SHA4-D/SHA483Q.Seq.d/                        155   5e-37
SFK778 (SFK778Q) /CSM/SF/SFK7-D/SFK778Q.Seq.d/                        155   5e-37
SFK753 (SFK753Q) /CSM/SF/SFK7-C/SFK753Q.Seq.d/                        155   5e-37
SFK673 (SFK673Q) /CSM/SF/SFK6-D/SFK673Q.Seq.d/                        155   5e-37
SFK593 (SFK593Q) /CSM/SF/SFK5-D/SFK593Q.Seq.d/                        155   5e-37
SFK451 (SFK451Q) /CSM/SF/SFK4-C/SFK451Q.Seq.d/                        155   5e-37
own update 2002.10.28
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

X51892|X51892.1 Dictyostelium discoideum SP60 gene for spore coat protein.   107  4e-78  4
AC115685|AC115685.1 Dictyostelium discoideum chromosome 2 map 4718821-4752388 strain AX4, complete sequence.   107  9e-75  4
AC116977|AC116977.2 Dictyostelium discoideum chromosome 2 map 5515173-5817331 strain AX4, complete sequence.   107  6e-72  4
M26239|M26239.1 D.discoideum spore coat protein SP60 gene, complete cds.   107  4e-59  3
X52105|X52105.1 Dictyostelium discoideum SP60 gene for spore coat protein.    80  6e-28  2
M34546|M34546.1 D.discoideum spore coat 60 (sp60) gene, 5' flank.    88  8e-14  1
AF078801|AF078801.1 Hordeum vulgare degenerate retrotransposon cereba polyprotein pseudogene, partial sequence.    48  0.072  1
BV025443|BV025443.1 S212P6456FC5.T0 CZECHII/Ei Mus musculus STS genomic, sequence tagged site.    42  0.13  2
BX571707|BX571707.1 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone  DKEY-93K4.    46  0.28  1
BQ546186|BQ546186.1 EST629813 TpMugugaSh01 Theileria parva cDNA clone TPFAU62, mRNA sequence.    44  1.1  1
dna update 2003. 7.22
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P15270) RecName: Full=Spore coat protein SP60; Flags: Precursor...    52   8e-06
protein update 2009. 3.26
PSORT 

 psg: 0.20  gvh: 0.51  alm: 0.60  top: 0.53  tms: 0.00  mit: 0.35  mip: 0.06
 nuc: 0.26  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 68.0 %: nuclear
	 24.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: mitochondrial

>> prediction for AFA618 is nuc
5' end seq. ID AFA618F
5' end seq. 
>AFA618F.Seq
ATTGTAGTATTTTGTTCATATTCAAATTAAATATCAATACAATTAAAAAAAGTTAAAATT
AAATTTGTAAAATCAATTTGTAACAAAAACTAGTAATTTAAAAAAAAAAAAACCCAAAAA
AAAAA----------
Length of 5' end seq. 125
3' end seq. ID AFA618Z
3' end seq. 
>AFA618Z.Seq
----------TCCANATTTCCATANATGTGTTNAAANACGTGGNGGNATNTTAAGNTGNG
AATTTNATCCNCCAANACAACCNAGATCCCTTGATTGGGCTGAAAATGAAAATGATGATC
GTGACTATGATGATCGNGNCTATGATGATGATGAANATGATGGTGATTATNATGGCCGNG
ACGGTGACTATGATGGNGATTATGATGGNGATTATGATGATGNCNATTATT
Length of 3' end seq. 221
Connected seq. ID AFA618P
Connected seq. 
>AFA618P.Seq
ATTGTAGTATTTTGTTCATATTCAAATTAAATATCAATACAATTAAAAAAAGTTAAAATT
AAATTTGTAAAATCAATTTGTAACAAAAACTAGTAATTTAAAAAAAAAAAAACCCAAAAA
AAAAA----------TCCANATTTCCATANATGTGTTNAAANACGTGGNGGNATNTTAAG
NTGNGAATTTNATCCNCCAANACAACCNAGATCCCTTGATTGGGCTGAAAATGAAAATGA
TGATCGTGACTATGATGATCGNGNCTATGATGATGATGAANATGATGGTGATTATNATGG
CCGNGACGGTGACTATGATGGNGATTATGATGGNGATTATGATGATGNCNATTATT
Length of connected seq. 346
Full length Seq ID -
Full length Seq. -
Length of full length seq. -