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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFF885
Library AF
(Link to library)
Clone ID AFF885
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID AFF885P
(Link to Original site)
Representative DNA sequence 
>AFF885 (AFF885Q) /CSM/AF/AFF8-D/AFF885Q.Seq.d/
AAATNATCATATTTATAATTATATCTTTTTTGTTTAATAAAAAAGAAAGAATACCAACAT
GAGACTTTTATTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATTTTGCATTATCAACAGT
TCATTTCAAAGATACCTTTGATAACGATTGGGAATCAAGATGGGTTGTTAGTGATTGGCA
TAAAGAAGATGGTAAATCAGGTAAATTAGTTCATACTGCAGGTAAATGGTTTGGTGATGA
AAATCAAAAAGGTATTCAAACTAGTGAAGATGCTCGTTTCTACGCTGTATCAGCTAAATT
CCCAAGTTTCAGTAACAAAGGTAAAGATTTAGTTCTCCAATACACTGTAAAGAATGAACA
AAAAGTTGATTGTGGTGGTTCATACATCAAGTTATTACCATCCAAATTAGATCAATCAGC
ATTCGATGGTGAATCTGAATATTCCATTATGTTTGGTCCAGATGTTTGTGGTGCCTCAAA
GAGAGTTCACGTCATTCTCAACTACAAAGGAAAGAACCATTTAATCAAGAAAGAAATCAA
XXXXXXXXXXCGAAGCCGCCAAAGCTGAANANANAAAGAAAGCNGNCGAAAAATTAGCCG
CCGAAAAAGCTGCTGAAAAAGAAGCCGAAGAAGCTGATGAAGAAGAAGAAGAAGTCNCTG
AANAAGATTTAGTTAAAACNGATGNCAAAAAAGAAGAAGNCNAAAAATCNACCNAAAAAG
TTG
sequence update 2001. 6. 2
Translated Amino Acid sequence 
nxhiynyiffv**KRKNTNMRLLLCLIFLVFVFNFALSTVHFKDTFDNDWESRWVVSDWH
KEDGKSGKLVHTAGKWFGDENQKGIQTSEDARFYAVSAKFPSFSNKGKDLVLQYTVKNEQ
KVDCGGSYIKLLPSKLDQSAFDGESEYSIMFGPDVCGASKRVHVILNYKGKNHLIKKEI-
--

---EAAKAEXXKKAXEKLAAEKAAEKEAEEADEEEEEVXEXDLVKTDXKKEEXXKSTXKV
Translated Amino Acid sequence (All Frames) 
Frame A:
kxsyl*lylfclikkkeyqhetfivfnflsfcfqfciinssfqryl**rlgikmgc**la
*rrw*ir*issycr*mvw**kskrysn**rcsflrcis*ipkfq*qr*rfsspihcke*t
ks*lwwfihqvitiqirsisirw*i*ifhyvwsrclwclkessrhsqlqrkepfnqernq
---

---rsrqs*xxkesxrkisrrksc*krsrrs**rrrrsx*xrfs*nxxqkrrxxkixxks

Frame B:
nxhiynyiffv**KRKNTNMRLLLCLIFLVFVFNFALSTVHFKDTFDNDWESRWVVSDWH
KEDGKSGKLVHTAGKWFGDENQKGIQTSEDARFYAVSAKFPSFSNKGKDLVLQYTVKNEQ
KVDCGGSYIKLLPSKLDQSAFDGESEYSIMFGPDVCGASKRVHVILNYKGKNHLIKKEI-
--

---EAAKAEXXKKAXEKLAAEKAAEKEAEEADEEEEEVXEXDLVKTDXKKEEXXKSTXKV

Frame C:
xiifiiisflfnkkeript*dfycv*fs*flfsilhyqqfiskiplitignqdgllvigi
kkmvnqvn*filqvnglvmkikkvfklvkmlvstlyqlnsqvsvtkvki*fsntl*rmnk
klivvvhtssyyhpn*inqhsmvnlniplclvqmfvvpqreftsfsttkerti*srkks-
--

---kppklxxxrkxxkn*ppkkllkkkpkklmkkkkkslxki*lkxmxkkkkxknxpkkl
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFN642 (VFN642Q) /CSM/VF/VFN6-B/VFN642Q.Seq.d/                        860   0.0
VFM885 (VFM885Q) /CSM/VF/VFM8-D/VFM885Q.Seq.d/                        860   0.0
VFM435 (VFM435Q) /CSM/VF/VFM4-B/VFM435Q.Seq.d/                        860   0.0
VFM276 (VFM276Q) /CSM/VF/VFM2-D/VFM276Q.Seq.d/                        860   0.0
VFL360 (VFL360Q) /CSM/VF/VFL3-C/VFL360Q.Seq.d/                        860   0.0
VFL283 (VFL283Q) /CSM/VF/VFL2-D/VFL283Q.Seq.d/                        860   0.0
VFL214 (VFL214Q) /CSM/VF/VFL2-A/VFL214Q.Seq.d/                        860   0.0
VFK631 (VFK631Q) /CSM/VF/VFK6-B/VFK631Q.Seq.d/                        860   0.0
VFK516 (VFK516Q) /CSM/VF/VFK5-A/VFK516Q.Seq.d/                        860   0.0
VFI619 (VFI619Q) /CSM/VF/VFI6-A/VFI619Q.Seq.d/                        860   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U36937|U36937.1 Dictyostelium discoideum calreticulin mRNA, complete cds.   860   0.0    3
BD248385|BD248385.1 Gene participating in tolerance against environmental stress.    92   1e-22  2
BZ392863|BZ392863.1 EINBW38TF EI_10_12_KB Entamoeba invadens genomic clone EINBW38, DNA sequence.    52   9e-07  2
BI876472|BI876472.1 fl72h01.y1 Sugano Kawakami zebrafish DRA Danio rerio cDNA clone 3819504 5' similar to TR:Q9PUC1 Q9PUC1 CALRETICULIN. ;, mRNA sequence.    42   5e-04  2
CB086220|CB086220.1 hj81f04.g1 Hedyotis centranthoides flower - Stage 2 (NYBG) Hedyotis centranthoides cDNA clone hj81f04, mRNA sequence.    36   6e-04  3
BI468042|BI468042.1 EST00452 Atlantic salmon Lambda Zap Express liver cDNA library Salmo salar cDNA clone LRR7-f02 5' similar to Calreticulin, mRNA sequence.    56   7e-04  1
CN181296|CN181296.1 SGP169588 Atlantic salmon Intestine cDNA library Salmo salar cDNA clone TH4-0159, mRNA sequence.    56   7e-04  1
CK420742|CK420742.1 AUF_IpTrk_27_j08 Trunk kidney cDNA library Ictalurus punctatus cDNA 5' similar to ER-resident chaperone calreticulin, mRNA sequence.    56   7e-04  1
AY342298|AY342298.1 Ictalurus punctatus ER-resident chaperone calreticulin mRNA, complete cds.    56   7e-04  1
CB937139|CB937139.1 IpCGJx13_12_E07_23 IpCGJx13 Ictalurus punctatus cDNA clone IpCGJx13_12_E07 5', mRNA sequence.    56   7e-04  1
dna update 2004. 4.28
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U36937_1(U36937|pid:none) Dictyostelium discoideum calreticulin ...   302   6e-81
AC133339_28(AC133339|pid:none) Oryza sativa chromosome 3 BAC OSJ...   196   8e-49
U66344_1(U66344|pid:none) Arabidopsis thaliana calreticulin (Crt...   193   4e-48
U27698_1(U27698|pid:none) Arabidopsis thaliana calreticulin (AtC...   193   4e-48
(Q40401) RecName: Full=Calreticulin; Flags: Precursor;  &T16968(...   193   4e-48
AB196794_1(AB196794|pid:none) Glycine max Gm crt-1 mRNA for calr...   191   1e-47
EF085019_1(EF085019|pid:none) Picea sitchensis clone WS0297_I16 ...   191   2e-47
EU984501_1(EU984501|pid:none) Nicotiana tabacum calreticulin mRN...   191   2e-47
AY086745_1(AY086745|pid:none) Arabidopsis thaliana clone 27210 m...   190   3e-47
AY836753_1(AY836753|pid:none) Triticum aestivum calreticulin-lik...   190   3e-47
protein update 2009. 4.20
PSORT 

 psg: 1.01  gvh: 0.69  alm: 0.49  top: 0.10  tms: 0.00  mit: 0.40  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: extracellular, including cell wall
	 12.0 %: cytoplasmic
	 12.0 %: mitochondrial
	  8.0 %: Golgi
	  8.0 %: vacuolar
	  8.0 %: nuclear
	  8.0 %: endoplasmic reticulum

>> prediction for AFF885 is exc
5' end seq. ID AFF885F
5' end seq. 
>AFF885F.Seq
AAATNATCATATTTATAATTATATCTTTTTTGTTTAATAAAAAAGAAAGAATACCAACAT
GAGACTTTTATTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATTTTGCATTATCAACAGT
TCATTTCAAAGATACCTTTGATAACGATTGGGAATCAAGATGGGTTGTTAGTGATTGGCA
TAAAGAAGATGGTAAATCAGGTAAATTAGTTCATACTGCAGGTAAATGGTTTGGTGATGA
AAATCAAAAAGGTATTCAAACTAGTGAAGATGCTCGTTTCTACGCTGTATCAGCTAAATT
CCCAAGTTTCAGTAACAAAGGTAAAGATTTAGTTCTCCAATACACTGTAAAGAATGAACA
AAAAGTTGATTGTGGTGGTTCATACATCAAGTTATTACCATCCAAATTAGATCAATCAGC
ATTCGATGGTGAATCTGAATATTCCATTATGTTTGGTCCAGATGTTTGTGGTGCCTCAAA
GAGAGTTCACGTCATTCTCAACTACAAAGGAAAGAACCATTTAATCAAGAAAGAAATCAA
----------
Length of 5' end seq. 540
3' end seq. ID AFF885Z
3' end seq. 
>AFF885Z.Seq
----------CGAAGCCGCCAAAGCTGAANANANAAAGAAAGCNGNCGAAAAATTAGCCG
CCGAAAAAGCTGCTGAAAAAGAAGCCGAAGAAGCTGATGAAGAAGAAGAAGAAGTCNCTG
AANAAGATTTAGTTAAAACNGATGNCAAAAAAGAAGAAGNCNAAAAATCNACCNAAAAAG
TTG
Length of 3' end seq. 173
Connected seq. ID AFF885P
Connected seq. 
>AFF885P.Seq
AAATNATCATATTTATAATTATATCTTTTTTGTTTAATAAAAAAGAAAGAATACCAACAT
GAGACTTTTATTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATTTTGCATTATCAACAGT
TCATTTCAAAGATACCTTTGATAACGATTGGGAATCAAGATGGGTTGTTAGTGATTGGCA
TAAAGAAGATGGTAAATCAGGTAAATTAGTTCATACTGCAGGTAAATGGTTTGGTGATGA
AAATCAAAAAGGTATTCAAACTAGTGAAGATGCTCGTTTCTACGCTGTATCAGCTAAATT
CCCAAGTTTCAGTAACAAAGGTAAAGATTTAGTTCTCCAATACACTGTAAAGAATGAACA
AAAAGTTGATTGTGGTGGTTCATACATCAAGTTATTACCATCCAAATTAGATCAATCAGC
ATTCGATGGTGAATCTGAATATTCCATTATGTTTGGTCCAGATGTTTGTGGTGCCTCAAA
GAGAGTTCACGTCATTCTCAACTACAAAGGAAAGAACCATTTAATCAAGAAAGAAATCAA
----------CGAAGCCGCCAAAGCTGAANANANAAAGAAAGCNGNCGAAAAATTAGCCG
CCGAAAAAGCTGCTGAAAAAGAAGCCGAAGAAGCTGATGAAGAAGAAGAAGAAGTCNCTG
AANAAGATTTAGTTAAAACNGATGNCAAAAAAGAAGAAGNCNAAAAATCNACCNAAAAAG
TTG
Length of connected seq. 713
Full length Seq ID -
Full length Seq. -
Length of full length seq. -