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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CHG688
Library CH
(Link to library)
Clone ID CHG688
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16419-1
Original site URL
Representative seq. ID CHG688P
(Link to Original site)
Representative DNA sequence 
>CHG688 (CHG688Q) /CSM/CH/CHG6-D/CHG688Q.Seq.d/
ATTCAATTTTATTTTTAATCAATTCAAATTAAAAAAAAAACAATAACTAACTAAGTTAAA
AATAAAAAAATAATAAAGGATGTTGTTTCTAAAAAATATTGGAGTATTTTTTATGATATT
CCTTGTATCAAAATCCTATGCGACGGATTGTAATAAAATTACTAATGAANAANAATGTCA
TAAATCTTCGGAATGTATTGTTATAAATTATACACCATGTTGTGGTGAACAAAAATGGGC
TTGCTCTAAAGGTACATTTGATACTTGTACATATGAAAATAGTTGTTATANAAATTCATC
CAATAACCAAGTTGTTGAAGTATCAAATAAATGCTTCAATCTTGATGGATTTATTAAAAT
TACAACTCCAACCGAGTATTCTTGTTCTGATGCCAANATTAAAGAATGTGCCTTATTAGG
TAAATCATGTAGTTTCCAAAANAACTCTTGTTCAAATCCAACTTCCTGTTGCCCAGGTGA
ATCAATTTGTGAAGGTTTAAGCTCTGGTAGTTCAACATCTGGTGGTGGTTCATCAGGTXX
XXXXXXXXTTGTTNTTTAAGATGTCCNCCNAAATCATGAATGTAGATTTAATGACCAAGG
TCATCAATGCTGTGTAAAGGTTCACCATGATAGATGTTCATTAAGATGTCCACATGCCCA
TGAATGTAAAGTTGATCNCAATGGTAAAGAATGTTGCGTCAGATCCCATAGACCACCACC
ACCAGAAGTTTGTTCATTAAGATGCCCACCAAAACATGAATGTAAATTTGATGATCATGG
TAAAAAATGTTGTGTAAAGATTCATTGTGATGAAGTTTGTGATTTAGATTGTGGTAGAGG
TTTTGAATGTAAAATTAGACATGATGGTTCAAAATGTTGTGTCCGTTCAGAAAGACCACA
CCCACCACAACATGAAAAATGTAATAAGAGATGCCCACCAGGCCATGAATGTAAAGTTGA
TCAACATGGAAAAGAATGTTGCGTTGTTGCCCATAGACCACCACCAAAATGCTCTTTAAG
ATGTCCACCAAGACATGAATGTAGAGTCAACCACTTTGGTGAAGAATGTTGTGTTAAAGT
TCACCACGATAAATGTTCATTGAGATGTCCACCAGGCCATGAATGTAAAGTTGATCAACA
TGGAAAAGAATGTTGCGTTGTTGCCCATAGACCACCACCAAAATGCTCATTAAGATGTCC
ACCAAAACATGAATGTAGAATCAATCACTTTGGTGAAGAATGCTGTGTTAAAAGTAGAAA
TGATTGTTTAACTTGTGAAGACNTAAACTGTGAAAGAAAAGGTTTACATTGTGCCATGAA
AACTGTACCAATTGATAAAGAAAATTGTTGGAAAAAGNA
sequence update 2002.10.25
Translated Amino Acid sequence 
fnfifnqfklkkkq*ltklkikk**RMLFLKNIGVFFMIFLVSKSYATDCNKITNEXXCH
KSSECIVINYTPCCGEQKWACSKGTFDTCTYENSCYXNSSNNQVVEVSNKCFNLDGFIKI
TTPTEYSCSDAXIKECALLGKSCSFQXNSCSNPTSCCPGESICEGLSSGSSTSGGGSSG-
--

---LXFKMSXXIMNVDLMTKVINAV*rftmidvh*dvhmpmnvklixmvknvasdpidhh
hqkfvh*dahqnmnvnlmimvknvv*rfivmkfvi*ivvevlnvkldmmvqnvvsvqkdh
thhnmknvirdahqamnvklinmeknvallpidhhqnal*dvhqdmnvesttlvknvvlk
fttinvh*dvhqamnvklinmeknvallpidhhqnah*dvhqnmnvesitlvknavlkve
miv*lvkt*tvkekvyivp*klyqlikkivgkx
Translated Amino Acid sequence (All Frames) 
Frame A:
iqfyf*siqikkktitn*vknkkiikdvvskkywsifydipcikilcdgl**ny**xxms
*ifgmycyklytmlw*tkmgll*ryi*ylyi*k*llxkfiq*psc*sik*mlqs*wiy*n
ynsnrvflf*cqx*rmclir*im*fpkxllfksnfllpr*inl*rfklw*fniwwwfir-
--

---LXFKMSXXIMNVDLMTKVINAV*rftmidvh*dvhmpmnvklixmvknvasdpidhh
hqkfvh*dahqnmnvnlmimvknvv*rfivmkfvi*ivvevlnvkldmmvqnvvsvqkdh
thhnmknvirdahqamnvklinmeknvallpidhhqnal*dvhqdmnvesttlvknvvlk
fttinvh*dvhqamnvklinmeknvallpidhhqnah*dvhqnmnvesitlvknavlkve
miv*lvkt*tvkekvyivp*klyqlikkivgkx

Frame B:
fnfifnqfklkkkq*ltklkikk**RMLFLKNIGVFFMIFLVSKSYATDCNKITNEXXCH
KSSECIVINYTPCCGEQKWACSKGTFDTCTYENSCYXNSSNNQVVEVSNKCFNLDGFIKI
TTPTEYSCSDAXIKECALLGKSCSFQXNSCSNPTSCCPGESICEGLSSGSSTSGGGSSG-
--

---cxlrcppks*m*i**prssmlckgsp**mfikmstcp*m*s*sqw*rmlrqip*ttt
trslfikmptkt*m*i**sw*kmlckdsl**sl*frlw*rf*m*n*t*wfkmlcpfrktt
pttt*km**emptrp*m*s*stwkrmlrccp*tttkmlfkmstkt*m*sqplw*rmlc*s
spr*mfiemstrp*m*s*stwkrmlrccp*tttkmlikmstkt*m*nqslw*rmlc*k*k
*lfnl*rxkl*kkrftlchenctn**rkllekx

Frame C:
silflinsn*kknnn*ls*k*knnkgccf*kileyfl*yslyqnpmrrivikllmxxnvi
nlrnvll*iihhvvvnknglalkvhlilvhmkivvixihpitkllkyqinasilmdllkl
qlqpsilvlmpxlknvpy*vnhvvskxtlvqiqlpvaqvnqfvkv*alvvqhlvvvhq--
-

---vx*dvxxnhecrfndqghqccvkvhhdrcslrcphaheckvdxngkeccvrshrppp
pevcslrcppkheckfddhgkkccvkihcdevcdldcgrgfeckirhdgskccvrserph
ppqhekcnkrcppgheckvdqhgkeccvvahrpppkcslrcpprhecrvnhfgeeccvkv
hhdkcslrcppgheckvdqhgkeccvvahrpppkcslrcppkhecrinhfgeeccvksrn
dcltcedxncerkglhcamktvpidkencwkk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHG688 (CHG688Q) /CSM/CH/CHG6-D/CHG688Q.Seq.d/                       2345   0.0
CHR406 (CHR406Q) /CSM/CH/CHR4-A/CHR406Q.Seq.d/                       1560   0.0
CHG345 (CHG345Q) /CSM/CH/CHG3-B/CHG345Q.Seq.d/                       1560   0.0
AHE263 (AHE263Q) /CSM/AH/AHE2-C/AHE263Q.Seq.d/                       1560   0.0
CHL140 (CHL140Q) /CSM/CH/CHL1-B/CHL140Q.Seq.d/                       1558   0.0
AHL722 (AHL722Q) /CSM/AH/AHL7-A/AHL722Q.Seq.d/                       1558   0.0
AHD145 (AHD145Q) /CSM/AH/AHD1-B/AHD145Q.Seq.d/                       1558   0.0
CHH267 (CHH267Q) /CSM/CH/CHH2-C/CHH267Q.Seq.d/                       1552   0.0
CHF268 (CHF268Q) /CSM/CH/CHF2-C/CHF268Q.Seq.d/                       1552   0.0
CHL853 (CHL853Q) /CSM/CH/CHL8-C/CHL853Q.Seq.d/                       1550   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U25144|U25144.1 Dictyostelium discoideum spore coat protein SP87 (PspD) gene, complete cds.  1526   0.0    11
AC117267|AC117267.2 Dictyostelium discoideum chromosome 2 map 5836255-5862024 strain AX4, complete sequence.  1526   0.0    12
M26239|M26239.1 D.discoideum spore coat protein SP60 gene, complete cds.    66   8e-15  4
X51892|X51892.1 Dictyostelium discoideum SP60 gene for spore coat protein.    66   2e-14  4
AC124167|AC124167.2 Tetraodon nigroviridis clone GSTNB-42J2, WORKING DRAFT SEQUENCE, 14 unordered pieces.    38   0.010  5
AC150239|AC150239.2 Sorex araneus clone SA_Ba-527L4, WORKING DRAFT SEQUENCE, 7 ordered pieces.    36   0.016  8
X52105|X52105.1 Dictyostelium discoideum SP60 gene for spore coat protein.    48   0.037  3
AC141955|AC141955.2 Rattus norvegicus clone CH230-418P10, WORKING DRAFT SEQUENCE, 29 unordered pieces.    48   0.13   4
AC146784|AC146784.21 Medicago truncatula clone mth2-172c4, WORKING DRAFT SEQUENCE, 2 ordered pieces.    34   0.17   7
AC127160|AC127160.3 Rattus norvegicus clone CH230-460D14, *** SEQUENCING IN PROGRESS ***, 1 ordered piece.    48   0.38   1
dna update 2004.10.22
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC117267_7(AC117267|pid:none) Dictyostelium discoideum chromosom...   615   e-174
(P15270) RecName: Full=Spore coat protein SP60; Flags: Precursor...   199   2e-49
X52105_1(X52105|pid:none) Dictyostelium discoideum SP60 gene for...   119   3e-25
(Q04503) RecName: Full=Prespore protein Dp87; Flags: Precursor;       108   4e-22
(Q6TU45) RecName: Full=Probable spore coat protein sigD; AltName...   107   9e-22
D13973_1(D13973|pid:none) Dictyostelium discoideum gene for Dp87...   104   8e-21
(Q54QX2) RecName: Full=Probable spore coat protein DDB_G0283555;...    87   1e-15
S07638(S07638;A60942;B60942) spore coat protein SP96 precursor -...    69   3e-10
(P14328) RecName: Full=Spore coat protein SP96;  &AC117075_49(AC...    69   3e-10
(P54704) RecName: Full=Spore coat protein SP85; AltName: Full=Ce...    68   6e-10
protein update 2009. 4. 1
PSORT 

 psg: 1.02  gvh: 0.68  alm: 0.47  top: 0.33  tms: 0.00  mit: 0.38  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: extracellular, including cell wall
	 12.0 %: mitochondrial
	  8.0 %: cytoplasmic
	  8.0 %: nuclear
	  8.0 %: endoplasmic reticulum
	  4.0 %: Golgi
	  4.0 %: vacuolar

>> prediction for CHG688 is exc
5' end seq. ID CHG688F
5' end seq. 
>CHG688F.Seq
ATTCAATTTTATTTTTAATCAATTCAAATTAAAAAAAAAACAATAACTAACTAAGTTAAA
AATAAAAAAATAATAAAGGATGTTGTTTCTAAAAAATATTGGAGTATTTTTTATGATATT
CCTTGTATCAAAATCCTATGCGACGGATTGTAATAAAATTACTAATGAANAANAATGTCA
TAAATCTTCGGAATGTATTGTTATAAATTATACACCATGTTGTGGTGAACAAAAATGGGC
TTGCTCTAAAGGTACATTTGATACTTGTACATATGAAAATAGTTGTTATANAAATTCATC
CAATAACCAAGTTGTTGAAGTATCAAATAAATGCTTCAATCTTGATGGATTTATTAAAAT
TACAACTCCAACCGAGTATTCTTGTTCTGATGCCAANATTAAAGAATGTGCCTTATTAGG
TAAATCATGTAGTTTCCAAAANAACTCTTGTTCAAATCCAACTTCCTGTTGCCCAGGTGA
ATCAATTTGTGAAGGTTTAAGCTCTGGTAGTTCAACATCTGGTGGTGGTTCATCAGGTNN
NNNNNNNN
Length of 5' end seq. 548
3' end seq. ID CHG688Z
3' end seq. 
>CHG688Z.Seq
NNNNNNNNNNTTGTTNTTTAAGATGTCCNCCNAAATCATGAATGTAGATTTAATGACCAA
GGTCATCAATGCTGTGTAAAGGTTCACCATGATAGATGTTCATTAAGATGTCCACATGCC
CATGAATGTAAAGTTGATCNCAATGGTAAAGAATGTTGCGTCAGATCCCATAGACCACCA
CCACCAGAAGTTTGTTCATTAAGATGCCCACCAAAACATGAATGTAAATTTGATGATCAT
GGTAAAAAATGTTGTGTAAAGATTCATTGTGATGAAGTTTGTGATTTAGATTGTGGTAGA
GGTTTTGAATGTAAAATTAGACATGATGGTTCAAAATGTTGTGTCCGTTCAGAAAGACCA
CACCCACCACAACATGAAAAATGTAATAAGAGATGCCCACCAGGCCATGAATGTAAAGTT
GATCAACATGGAAAAGAATGTTGCGTTGTTGCCCATAGACCACCACCAAAATGCTCTTTA
AGATGTCCACCAAGACATGAATGTAGAGTCAACCACTTTGGTGAAGAATGTTGTGTTAAA
GTTCACCACGATAAATGTTCATTGAGATGTCCACCAGGCCATGAATGTAAAGTTGATCAA
CATGGAAAAGAATGTTGCGTTGTTGCCCATAGACCACCACCAAAATGCTCATTAAGATGT
CCACCAAAACATGAATGTAGAATCAATCACTTTGGTGAAGAATGCTGTGTTAAAAGTAGA
AATGATTGTTTAACTTGTGAAGACNTAAACTGTGAAAGAAAAGGTTTACATTGTGCCATG
AAAACTGTACCAATTGATAAAGAAAATTGTTGGAAAAAGNA
Length of 3' end seq. 821
Connected seq. ID CHG688P
Connected seq. 
>CHG688P.Seq
ATTCAATTTTATTTTTAATCAATTCAAATTAAAAAAAAAACAATAACTAACTAAGTTAAA
AATAAAAAAATAATAAAGGATGTTGTTTCTAAAAAATATTGGAGTATTTTTTATGATATT
CCTTGTATCAAAATCCTATGCGACGGATTGTAATAAAATTACTAATGAANAANAATGTCA
TAAATCTTCGGAATGTATTGTTATAAATTATACACCATGTTGTGGTGAACAAAAATGGGC
TTGCTCTAAAGGTACATTTGATACTTGTACATATGAAAATAGTTGTTATANAAATTCATC
CAATAACCAAGTTGTTGAAGTATCAAATAAATGCTTCAATCTTGATGGATTTATTAAAAT
TACAACTCCAACCGAGTATTCTTGTTCTGATGCCAANATTAAAGAATGTGCCTTATTAGG
TAAATCATGTAGTTTCCAAAANAACTCTTGTTCAAATCCAACTTCCTGTTGCCCAGGTGA
ATCAATTTGTGAAGGTTTAAGCTCTGGTAGTTCAACATCTGGTGGTGGTTCATCAGGT--
--------TTGTTNTTTAAGATGTCCNCCNAAATCATGAATGTAGATTTAATGACCAAGG
TCATCAATGCTGTGTAAAGGTTCACCATGATAGATGTTCATTAAGATGTCCACATGCCCA
TGAATGTAAAGTTGATCNCAATGGTAAAGAATGTTGCGTCAGATCCCATAGACCACCACC
ACCAGAAGTTTGTTCATTAAGATGCCCACCAAAACATGAATGTAAATTTGATGATCATGG
TAAAAAATGTTGTGTAAAGATTCATTGTGATGAAGTTTGTGATTTAGATTGTGGTAGAGG
TTTTGAATGTAAAATTAGACATGATGGTTCAAAATGTTGTGTCCGTTCAGAAAGACCACA
CCCACCACAACATGAAAAATGTAATAAGAGATGCCCACCAGGCCATGAATGTAAAGTTGA
TCAACATGGAAAAGAATGTTGCGTTGTTGCCCATAGACCACCACCAAAATGCTCTTTAAG
ATGTCCACCAAGACATGAATGTAGAGTCAACCACTTTGGTGAAGAATGTTGTGTTAAAGT
TCACCACGATAAATGTTCATTGAGATGTCCACCAGGCCATGAATGTAAAGTTGATCAACA
TGGAAAAGAATGTTGCGTTGTTGCCCATAGACCACCACCAAAATGCTCATTAAGATGTCC
ACCAAAACATGAATGTAGAATCAATCACTTTGGTGAAGAATGCTGTGTTAAAAGTAGAAA
TGATTGTTTAACTTGTGAAGACNTAAACTGTGAAAGAAAAGGTTTACATTGTGCCATGAA
AACTGTACCAATTGATAAAGAAAATTGTTGGAAAAAGNA
Length of connected seq. 1349
Full length Seq ID -
Full length Seq. -
Length of full length seq. -