>CHG368 (CHG368Q) /CSM/CH/CHG3-C/CHG368Q.Seq.d/
XXXXXXXXXXTTCTCGAAGATGTAAAGAAAATCGCTACNTCACAAAAAGGTAATGCCGTT
TTCTGTTGGGTCGATATGAAGAAATTCCCACAACAAGCAACTCATATGGGTCTCTCTGGT
AAAGTTGTTCCAGCCATCTCTGTTGATTCAGTTGCCAATAAAGCTCGTTACAATTTCGAT
GAAAAAGAAACCTTCTCATTCGATACCGTCTCCAAATGGATCCAAGATGTCATTGGTGGT
AAAGTTTCTCCATTTGTTAAATCTCAACCAGAACCAGAATCAAATGATGCTCCAGTTAAG
GTTGCCGTTGGTACCACTTTCAAAAAGTTGGTTTTAGACTCACCAAAGGATGTTTTGGTT
GAATTCTACGCTCCATGGTGCGGTCATTGCAAGAATCTTGCTCCAATCTACGATAAATTA
GGTGAATACTTAAAAGACGTTGAATCTGTCTCAATCGTAAAGATTGATGCCGACTCAAAT
GATGTACCAAGTGATATCGAAATTAGAGGTTACCCAACCATCATGCTCTTCAAAGCTGAT
GACAAAGAAAATCCAATCTCTTATGAAGGTCAAAGAAATGATCATATGAACTTTGTTGAA
TTCATTCAAGATAACGCTGCCATTGAATTCAAATTACCATCATCTC

---LEDVKKIATSQKGNAVFCWVDMKKFPQQATHMGLSGKVVPAISVDSVANKARYNFDE
KETFSFDTVSKWIQDVIGGKVSPFVKSQPEPESNDAPVKVAVGTTFKKLVLDSPKDVLVE
FYAPWCGHCKNLAPIYDKLGEYLKDVESVSIVKIDADSNDVPSDIEIRGYPTIMLFKADD
KENPISYEGQRNDHMNFVEFIQDNAAIEFKLPSS

Frame A:
---srrckenryxtkr*crfllgryeeipttsnsygslw*scsshlc*fscq*sslqfr*
krnlliryrlqmdprchww*sfsic*istrtrik*css*gcrwyhfqkvgfrltkgcfg*
ilrsmvrslqescsnlr*ir*ilkrr*iclnrkd*crlk*ctk*yrn*rlpnhhalqs**
qrksnll*rskk*syelc*ihsr*rch*iqitii


Frame B:
---LEDVKKIATSQKGNAVFCWVDMKKFPQQATHMGLSGKVVPAISVDSVANKARYNFDE
KETFSFDTVSKWIQDVIGGKVSPFVKSQPEPESNDAPVKVAVGTTFKKLVLDSPKDVLVE
FYAPWCGHCKNLAPIYDKLGEYLKDVESVSIVKIDADSNDVPSDIEIRGYPTIMLFKADD
KENPISYEGQRNDHMNFVEFIQDNAAIEFKLPSS


Frame C:
---skm*rkslxhkkvmpfsvgsi*rnshnkqliwvslvklfqpslliqlpiklvtismk
kkpshsipspngskmslvvkflhllnlnqnqnqmmlqlrlplvplskswf*thqrmfwln
stlhgaviarillqstin*vnt*ktlnlsqs*rlmptqmmyqvisklevtqpscssklmt
kkiqslmkvkemii*tllnsfkitlplnsnyhhl

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHG368 (CHG368Q) /CSM/CH/CHG3-C/CHG368Q.Seq.d/                       1255   0.0
AHO764 (AHO764Q) /CSM/AH/AHO7-C/AHO764Q.Seq.d/                       1255   0.0
AFO829 (AFO829Q) /CSM/AF/AFO8-B/AFO829Q.Seq.d/                       1255   0.0
AFO686 (AFO686Q) /CSM/AF/AFO6-D/AFO686Q.Seq.d/                       1255   0.0
AFO368 (AFO368Q) /CSM/AF/AFO3-C/AFO368Q.Seq.d/                       1255   0.0
AFO247 (AFO247Q) /CSM/AF/AFO2-B/AFO247Q.Seq.d/                       1255   0.0
AFN894 (AFN894Q) /CSM/AF/AFN8-D/AFN894Q.Seq.d/                       1255   0.0
AFN855 (AFN855Q) /CSM/AF/AFN8-C/AFN855Q.Seq.d/                       1255   0.0
AFN679 (AFN679Q) /CSM/AF/AFN6-D/AFN679Q.Seq.d/                       1255   0.0
AFN674 (AFN674Q) /CSM/AF/AFN6-D/AFN674Q.Seq.d/                       1255   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

BJ171852|BJ171852.1 Physcomitrella patens subsp. patens cDNA clone:pph30f12, 3' end,single read.   599   e-167  1
CN206669|CN206669.1 Tor7097 Gametophyte rehydration Library Tortula ruralis cDNA, mRNA sequence.    78   2e-10  1
CN591465|CN591465.1 TTE00010235 Normalized large Tetrahymena thermophila cDNA, mRNA sequence.    74   3e-09  1
BM398179|BM398179.1 5009-0-41-G11.t.1 Chilcoat/Turkewitz cDNA (large fraction) Tetrahymena thermophila cDNA, mRNA sequence.    74   3e-09  1
CN597971|CN597971.1 TTE00009997 Normalized large Tetrahymena thermophila cDNA, mRNA sequence.    74   3e-09  1
CN590755|CN590755.1 TTE00006978 Normalized large Tetrahymena thermophila cDNA, mRNA sequence.    74   3e-09  1
CB176061|CB176061.1 pl17e07.y3 Ancylostoma ceylanicum adult Ancylostoma ceylanicum cDNA 5' similar to TR:O17908 O17908 H06O01.1 PROTEIN. [1] ;, mRNA sequence.    70   5e-08  1
CN206834|CN206834.1 Tor7258 Gametophyte rehydration Library Tortula ruralis cDNA, mRNA sequence.    70   5e-08  1
CK030146|CK030146.1 AGENCOURT_16621384 NIH_ZGC_7 Danio rerio cDNA clone IMAGE:7052631 5', mRNA sequence.    70   5e-08  1
AL924030|AL924030.1 Danio rerio EST sequence, clone 134-B07-2.     70   5e-08  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q12730) RecName: Full=Protein disulfide-isomerase;          Sho...   173   4e-42
EU016183_1(EU016183|pid:none) Haemaphysalis longicornis strain G...   173   4e-42
(Q00248) RecName: Full=Protein disulfide-isomerase;          Sho...   169   8e-41
DQ066336_1(DQ066336|pid:none) Ixodes scapularis isolate IS-6-12-...   168   1e-40
DQ377176_1(DQ377176|pid:none) Amblyomma variegatum protein disul...   163   4e-39
CU633901_146(CU633901|pid:none) Podospora anserina genomic DNA c...   162   8e-39
AE013599_1413(AE013599|pid:none) Drosophila melanogaster chromos...   154   2e-36
AB022013_1(AB022013|pid:none) Antheraea pernyi mRNA for protein ...   151   1e-35
AK290971_1(AK290971|pid:none) Homo sapiens cDNA FLJ78033 complet...   150   2e-35
BC046707_1(BC046707|pid:none) Xenopus laevis glucose regulated p...   150   2e-35

 psg: 0.65  gvh: 0.36  alm: 0.43  top: 0.53  tms: 0.00  mit: 0.21  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 40.0 %: nuclear
	  8.0 %: cytoskeletal
	  8.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for CHG368 is cyt

>CHG368Z.Seq
NNNNNNNNNNTTCTCGAAGATGTAAAGAAAATCGCTACNTCACAAAAAGGTAATGCCGTT
TTCTGTTGGGTCGATATGAAGAAATTCCCACAACAAGCAACTCATATGGGTCTCTCTGGT
AAAGTTGTTCCAGCCATCTCTGTTGATTCAGTTGCCAATAAAGCTCGTTACAATTTCGAT
GAAAAAGAAACCTTCTCATTCGATACCGTCTCCAAATGGATCCAAGATGTCATTGGTGGT
AAAGTTTCTCCATTTGTTAAATCTCAACCAGAACCAGAATCAAATGATGCTCCAGTTAAG
GTTGCCGTTGGTACCACTTTCAAAAAGTTGGTTTTAGACTCACCAAAGGATGTTTTGGTT
GAATTCTACGCTCCATGGTGCGGTCATTGCAAGAATCTTGCTCCAATCTACGATAAATTA
GGTGAATACTTAAAAGACGTTGAATCTGTCTCAATCGTAAAGATTGATGCCGACTCAAAT
GATGTACCAAGTGATATCGAAATTAGAGGTTACCCAACCATCATGCTCTTCAAAGCTGAT
GACAAAGAAAATCCAATCTCTTATGAAGGTCAAAGAAATGATCATATGAACTTTGTTGAA
TTCATTCAAGATAACGCTGCCATTGAATTCAAATTACCATCATCTC