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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFF786
Library AF
(Link to library)
Clone ID AFF786
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16501-1
Original site URL
Representative seq. ID AFF786P
(Link to Original site)
Representative DNA sequence 
>AFF786 (AFF786Q) /CSM/AF/AFF7-D/AFF786Q.Seq.d/
TGGCCTACTGGAAAAAAAAAACTTTTTAATTTTCTCAAAATTAAGATAGTTGAACATGCA
AATTACAGAAAAAGAAACAGTNCAAGAAGAACAACAAAAATTACTATATCCACCACCTTC
TTTTAGTACAAAATGTCATATATCATCAGTAGAACAATATAATGAAATGTATAAAGAAAG
TATTGAATCACCAAATCAATTTTGGGATAAAAAAGCTAAAGAATTTTTAACTTGGTTTTC
TGATTATACAACTGTTCAACATGGATCATTCGAAAAAGGTGATATTTCATGGTTTTTAAA
TGGTAAAATTAATGTTTCATATAATTGTATCGATAGACATTTAAAAGAGAATGCAGATAA
AGTTGCAATACTATTTGAAGGTGATGAANAAACAATGGTTAAAAAAGTTACATATAGAGA
GATGTTTGAAGAGGTTTGTAGATTATCAAATCTTTTGATATCACTTGGCGTTGGTAAAGG
AGATACGGTAGCGATCTATCTACCAAACACACCAACCGCCATTTATTCAATGTTAGCATG
CGCTAGAATTGGAGCAATXXXXXXXXXXAGATTAGGTACTGCTGAATTAAAGAGTGCCCT
CGTTGGTTGTTCAATTTGTGCAGAGGCTGCCGTTGTTGGTTATCCTCATGATATCAAAGG
TCAAGGTATTTTAGCATTTTGTACACTCAAAGAAGGTTATCAAGAGGATGAATCAAATGT
TATTATGATGTTAAAAAAAGAAGTTAGAAATGTAATTGGTCCATTTGCAACACCTGATGT
AATTGTTATAACACCTTCACTTCCAAAAACTCGTAGTGGTAAAATTATGAGAAGAATTCT
TAGAAAAATTGGTTGTCATGAATCTTNCTGCTGAACAATTAGGTGATATTTCAACNTTAG
CTGAACCTGAAGTTGTCAAACTTTAATTGAAAAAGTTTCAAAAGTAATNCCAAAAAC
sequence update 2001. 6. 2
Translated Amino Acid sequence 
gllekknflifsklr*LNMQITEKETVQEEQQKLLYPPPSFSTKCHISSVEQYNEMYKES
IESPNQFWDKKAKEFLTWFSDYTTVQHGSFEKGDISWFLNGKINVSYNCIDRHLKENADK
VAILFEGDEXTMVKKVTYREMFEEVCRLSNLLISLGVGKGDTVAIYLPNTPTAIYSMLAC
ARIGA---

---RLGTAELKSALVGCSICAEAAVVGYPHDIKGQGILAFCTLKEGYQEDESNVIMMLKK
EVRNVIGPFATPDVIVITPSLPKTRSGKIMRRILRKIGCHESXC*tir*yfnxs*t*scq
tliekvskvxpk
Translated Amino Acid sequence (All Frames) 
Frame A:
wptgkkklfnflkikivehanyrkrnsxrrttkitisttff*ykmsyiisrti**nv*rk
y*itksilg*ks*rifnlvf*lyncstwiirkr*yfmvfkw*n*cfi*lyr*tfkrecr*
scnti*r**xnng*ksyi*rdv*rgl*iiksfditwrw*rrygsdlstkhtnrhlfnvsm
r*nwsn---

---RLGTAELKSALVGCSICAEAAVVGYPHDIKGQGILAFCTLKEGYQEDESNVIMMLKK
EVRNVIGPFATPDVIVITPSLPKTRSGKIMRRILRKIGCHESXC*tir*yfnxs*t*scq
tliekvskvxpk

Frame B:
gllekknflifsklr*LNMQITEKETVQEEQQKLLYPPPSFSTKCHISSVEQYNEMYKES
IESPNQFWDKKAKEFLTWFSDYTTVQHGSFEKGDISWFLNGKINVSYNCIDRHLKENADK
VAILFEGDEXTMVKKVTYREMFEEVCRLSNLLISLGVGKGDTVAIYLPNTPTAIYSMLAC
ARIGA---

---d*vlln*rvpslvvqfvqrlpllvilmiskvkvf*hfvhskkvikrmnqmll*c*kk
klem*lvhlqhlm*ll*hlhfqklvvvkl*eefleklvvmnlxaeqlgdistlaepevvk
l*lkkfqk*xqk

Frame C:
aywkkktf*fsqn*ds*tcklqkkkqxkknnknyyihhlllvqnviyhq*nnimkcikkv
lnhqinfgikklknf*lgfliiqlfnmdhskkvifhgf*mvklmfhiivsidi*krmqik
lqyylkvmxkqwlkklhierclkrfvdyqif*yhlalvkeir*rsiyqthqppfiqc*ha
leleq---

---iryc*ikecprwlfnlcrgcrcwlss*yqrsryfsilytqrrlsrg*ikcyydvkkr
s*kcnwsicnt*cncyntftskns*w*nyekns*knwls*ixllnn*vifqx*lnlklsn
fn*ksfksnxkn
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFF786 (AFF786Q) /CSM/AF/AFF7-D/AFF786Q.Seq.d/                       1725   0.0
VHE880 (VHE880Q) /CSM/VH/VHE8-D/VHE880Q.Seq.d/                       1023   0.0
VHE704 (VHE704Q) /CSM/VH/VHE7-A/VHE704Q.Seq.d/                       1023   0.0
AFE757 (AFE757Q) /CSM/AF/AFE7-C/AFE757Q.Seq.d/                       1023   0.0
VHO673 (VHO673Q) /CSM/VH/VHO6-D/VHO673Q.Seq.d/                       1015   0.0
VHJ869 (VHJ869Q) /CSM/VH/VHJ8-C/VHJ869Q.Seq.d/                       1011   0.0
VHD191 (VHD191Q) /CSM/VH/VHD1-D/VHD191Q.Seq.d/                       1011   0.0
CFI106 (CFI106Q) /CSM/CF/CFI1-A/CFI106Q.Seq.d/                       1011   0.0
CFH854 (CFH854Q) /CSM/CF/CFH8-C/CFH854Q.Seq.d/                       1011   0.0
SFB864 (SFB864Q) /CSM/SF/SFB8-C/SFB864Q.Seq.d/                        985   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC115612|AC115612.2 Dictyostelium discoideum chromosome 2 map 6245135-6357017 strain AX4, complete sequence.   579   0.0    9
AX489103|AX489103.1 Sequence 6403 from Patent WO02053728.          46   4e-05  4
BI452123|BI452123.1 GI05_D08 Glomus intraradices extra-radical mycelium Glomus intraradices cDNA clone GI05_D08 3', mRNA sequence.    60   6e-05  1
CB081202|CB081202.1 hk47f02.g1 Gnetum leaves (NYBG) Gnetum gnemon cDNA clone hk47f02, mRNA sequence.    60   6e-05  1
BX572091|BX572091.1 Prochlorococcus marinus MED4 complete genome; segment 2/5.    54   0.004  1
BX957220|BX957220.1 Methanococcus maripaludis strain S2, complete sequence; segment 2/5.    36   0.006  12
AP000983|AP000983.1 Sulfolobus tokodaii genomic DNA, complete sequence, section:3/10.    50   0.009  8
AR337814|AR337814.1 Sequence 1 from patent US 6566584.             40   0.045  2
CA148970|CA148970.1 SCJLRZ1021D03.g RZ1 Saccharum officinarum cDNA clone SCJLRZ1021D03 5', mRNA sequence.    40   0.047  2
CF635493|CF635493.1 zmrww00_0A20-011-b10.s2 zmrww00 Zea mays cDNA, mRNA sequence.    40   0.047  2
dna update 2004. 4.26
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC115612_37(AC115612|pid:none) Dictyostelium discoideum chromoso...   314   3e-84
(Q54Z60) RecName: Full=Acetyl-coenzyme A synthetase;          EC...   314   3e-84
(Q01576) RecName: Full=Acetyl-coenzyme A synthetase;          EC...   175   2e-42
(P16929) RecName: Full=Acetyl-coenzyme A synthetase;          EC...   162   1e-38
(P36333) RecName: Full=Acetyl-coenzyme A synthetase;          EC...   162   2e-38
A18007_1(A18007|pid:none) FacA gene seq ID No:1.                      162   2e-38
AM236082_44(AM236082|pid:none) Rhizobium leguminosarum bv. vicia...   160   7e-38
CP000934_2904(CP000934|pid:none) Cellvibrio japonicus Ueda107, c...   160   7e-38
CP000744_5378(CP000744|pid:none) Pseudomonas aeruginosa PA7, com...   159   1e-37
FM209186_5118(FM209186|pid:none) Pseudomonas aeruginosa LESB58 c...   158   2e-37
protein update 2009. 4.20
PSORT 

 psg: 0.75  gvh: 0.32  alm: 0.35  top: 0.53  tms: 0.00  mit: 0.23  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 40.0 %: cytoplasmic
	 12.0 %: mitochondrial
	  4.0 %: plasma membrane

>> prediction for AFF786 is nuc
5' end seq. ID AFF786F
5' end seq. 
>AFF786F.Seq
TGGCCTACTGGAAAAAAAAAACTTTTTAATTTTCTCAAAATTAAGATAGTTGAACATGCA
AATTACAGAAAAAGAAACAGTNCAAGAAGAACAACAAAAATTACTATATCCACCACCTTC
TTTTAGTACAAAATGTCATATATCATCAGTAGAACAATATAATGAAATGTATAAAGAAAG
TATTGAATCACCAAATCAATTTTGGGATAAAAAAGCTAAAGAATTTTTAACTTGGTTTTC
TGATTATACAACTGTTCAACATGGATCATTCGAAAAAGGTGATATTTCATGGTTTTTAAA
TGGTAAAATTAATGTTTCATATAATTGTATCGATAGACATTTAAAAGAGAATGCAGATAA
AGTTGCAATACTATTTGAAGGTGATGAANAAACAATGGTTAAAAAAGTTACATATAGAGA
GATGTTTGAAGAGGTTTGTAGATTATCAAATCTTTTGATATCACTTGGCGTTGGTAAAGG
AGATACGGTAGCGATCTATCTACCAAACACACCAACCGCCATTTATTCAATGTTAGCATG
CGCTAGAATTGGAGCAAT----------
Length of 5' end seq. 558
3' end seq. ID AFF786Z
3' end seq. 
>AFF786Z.Seq
----------AGATTAGGTACTGCTGAATTAAAGAGTGCCCTCGTTGGTTGTTCAATTTG
TGCAGAGGCTGCCGTTGTTGGTTATCCTCATGATATCAAAGGTCAAGGTATTTTAGCATT
TTGTACACTCAAAGAAGGTTATCAAGAGGATGAATCAAATGTTATTATGATGTTAAAAAA
AGAAGTTAGAAATGTAATTGGTCCATTTGCAACACCTGATGTAATTGTTATAACACCTTC
ACTTCCAAAAACTCGTAGTGGTAAAATTATGAGAAGAATTCTTAGAAAAATTGGTTGTCA
TGAATCTTNCTGCTGAACAATTAGGTGATATTTCAACNTTAGCTGAACCTGAAGTTGTCA
AACTTTAATTGAAAAAGTTTCAAAAGTAATNCCAAAAAC
Length of 3' end seq. 389
Connected seq. ID AFF786P
Connected seq. 
>AFF786P.Seq
TGGCCTACTGGAAAAAAAAAACTTTTTAATTTTCTCAAAATTAAGATAGTTGAACATGCA
AATTACAGAAAAAGAAACAGTNCAAGAAGAACAACAAAAATTACTATATCCACCACCTTC
TTTTAGTACAAAATGTCATATATCATCAGTAGAACAATATAATGAAATGTATAAAGAAAG
TATTGAATCACCAAATCAATTTTGGGATAAAAAAGCTAAAGAATTTTTAACTTGGTTTTC
TGATTATACAACTGTTCAACATGGATCATTCGAAAAAGGTGATATTTCATGGTTTTTAAA
TGGTAAAATTAATGTTTCATATAATTGTATCGATAGACATTTAAAAGAGAATGCAGATAA
AGTTGCAATACTATTTGAAGGTGATGAANAAACAATGGTTAAAAAAGTTACATATAGAGA
GATGTTTGAAGAGGTTTGTAGATTATCAAATCTTTTGATATCACTTGGCGTTGGTAAAGG
AGATACGGTAGCGATCTATCTACCAAACACACCAACCGCCATTTATTCAATGTTAGCATG
CGCTAGAATTGGAGCAAT----------AGATTAGGTACTGCTGAATTAAAGAGTGCCCT
CGTTGGTTGTTCAATTTGTGCAGAGGCTGCCGTTGTTGGTTATCCTCATGATATCAAAGG
TCAAGGTATTTTAGCATTTTGTACACTCAAAGAAGGTTATCAAGAGGATGAATCAAATGT
TATTATGATGTTAAAAAAAGAAGTTAGAAATGTAATTGGTCCATTTGCAACACCTGATGT
AATTGTTATAACACCTTCACTTCCAAAAACTCGTAGTGGTAAAATTATGAGAAGAATTCT
TAGAAAAATTGGTTGTCATGAATCTTNCTGCTGAACAATTAGGTGATATTTCAACNTTAG
CTGAACCTGAAGTTGTCAAACTTTAATTGAAAAAGTTTCAAAAGTAATNCCAAAAAC
Length of connected seq. 947
Full length Seq ID -
Full length Seq. -
Length of full length seq. -