>AFF763 (AFF763Q) /CSM/AF/AFF7-C/AFF763Q.Seq.d/
AATATTTTAATAATTATAATTTTAAAAAATGAGTATTGAATCAATTAAAGCTAGAGAAAT
TTTGGATAGTAGAGGTAATCCAACATTAGAAGTTGAAGTATGGACAAGTGAAGGAATGTT
TTCAGCCAAAGTACCAAGTGGAGCATCAACTGGTATTCATGAAGCATTTGAATTAAGAGA
TGGTGATAAAAGTCGTTATTTTGGATATGGTGTTTTGAATGCAATTGTTAATGTAAATAA
TTTAATATCACCAACATTGAAAGGTATGAGAGTTGATAATCAAAAAGCAATAGATGATAA
AATGATTGAATTGGATGGTACAAAGAATAAATCAAAACTTGGGTCCAATGCAATCGTTGG
TGTATCAATGGCAGTGGCAAGAGCAGCCGCTTCTATTAAAGGTGTGCCCTTGTACCGTTA
CATTTCACAATTGGCTGGAAATAGTAATATTCGTTTACCAGTACCATGTTTTAATGTTAT
TAATGGTGGAAAACATGCAGGTAATAGACTACCATTTCAAGAGTTTATGCTAGTACCCAT
TGGTGCACCAAAATTTAAAGAAGCATTGCGTTATGGTTCAGAAGTCTACCATTCACTCAA
AGAGATCATCAAGGAAAGAXXXXXXXXXXTCATTCGGGCTATCAAGATGTTATTAAAATT
GGGTATTGATTCTGCTGCTTCAGAATTTTGGAATCCAAAGACTTCAAAATATGATATGGA
NTTCAAGAATATAGGATCCTCACATTTGAAGACTAGTTTAAATAGTGGTGAGCAATTAAT
GTCTAGATATTTAGAGATTTTAAAAAAGTATCCAATTGCATTCTTTGAAGATCCATTTGG
TGAAGATGATTGGGAAAATCATGGTAAAATTACAGCTGCAATTGGTAATAAAATTCAAAT
CATTGGTGATGATTTACTTTGTACAAATCCAGAAAGAATTAAAAAAGCAATTTCTGAGAA
AACTGTAAATTCATTATTATTGAAAATAAATCAAATCGGTACACTAACTGAAACTATTGA
AGCTGCAAAACTTGCAAAAGAAGCTGGTTGGGGTTGTTTAGTTAGTCATCGTTCAGGTGA
AACTGATGATCATTTCATTGCTGATCTTGTAGTTGGTNCTTGGTACTGGTGAAATAAAAT
NCTGGTGCACCTTGTAGATTTGAAAGATTATCAAAATATAATAGATTAATGAAAATTGAA
GAAGAACCTGAAAAACTCATAGATCAAATGGTAATTCATTTGAGTATGCTGGTGAAGNAG
TTTTATNCATTTTAAAAAAATAAAAAAAANNAAAAA

if**l*f*KMSIESIKAREILDSRGNPTLEVEVWTSEGMFSAKVPSGASTGIHEAFELRD
GDKSRYFGYGVLNAIVNVNNLISPTLKGMRVDNQKAIDDKMIELDGTKNKSKLGSNAIVG
VSMAVARAAASIKGVPLYRYISQLAGNSNIRLPVPCFNVINGGKHAGNRLPFQEFMLVPI
GAPKFKEALRYGSEVYHSLKEIIKER---

---IRAIKMLLKLGIDSAASEFWNPKTSKYDMXFKNIGSSHLKTSLNSGEQLMSRYLEIL
KKYPIAFFEDPFGEDDWENHGKITAAIGNKIQIIGDDLLCTNPERIKKAISEKTVNSLLL
KINQIGTLTETIEAAKLAKEAGWGCLVSHRSGETDDHFIADLVVGXWYW*nkxlvhlvdl
kdyqniid**klkknlkns*ikw*fi*vcw*xsfxhfkkikkxk

Frame A:
niliiiilkney*in*s*rnfg**r*snirs*smdk*rnvfsqstkwsinwys*si*ikr
w**kslfwiwcfecnc*ck*fnitnieryes**sksnr**nd*igwyke*iktwvqcnrw
cingsgkssrfy*rcalvplhftigwk**ysftstmf*cy*wwktcr**ttisrvyasth
wctki*rsialwfrslpftqrdhqgk---

---sfglsrcy*nwvlilllqnfgiqrlqnmiwxsri*dphi*rlv*ivvsn*cldi*rf
*ksiqlhslkihlvkmigkimvklqlqlvikfkslvmiyfvqiqkelkkqflrkl*ihyy
*k*iksvh*lkllklqnlqkklvgvv*lvivqvklmiisllil*lvlgtgeikxwctl*i
*kiiki**inen*rrt*kthrsngnsfeyagexvlxilkk*kkxk

Frame B:
if**l*f*KMSIESIKAREILDSRGNPTLEVEVWTSEGMFSAKVPSGASTGIHEAFELRD
GDKSRYFGYGVLNAIVNVNNLISPTLKGMRVDNQKAIDDKMIELDGTKNKSKLGSNAIVG
VSMAVARAAASIKGVPLYRYISQLAGNSNIRLPVPCFNVINGGKHAGNRLPFQEFMLVPI
GAPKFKEALRYGSEVYHSLKEIIKER---

---hsgyqdvikigy*fccfrileskdfki*ygxqeyriltfed*fk*w*ainv*ifrdf
kkvsncil*rsiw*r*lgksw*nyscnw**nsnhw**ftlyksrkn*ksnf*enckfiii
enksnrytn*ny*scktckrswlglfs*ssfr*n**sfhc*scswxlvlvk*nxgapcrf
erlskynrlmkieeepeklidqmvihlsmlvkxfyxf*knkkxxk

Frame C:
yfnnynfkk*vlnqlklekfwiveviqh*klkygqvkecfqpkyqvehqlvfmkhln*em
vikvvildmvf*mqllm*ii*yhqh*kv*eliikkq*mik*lnwmvqrinqnlgpmqslv
yqwqwqeqplllkvcpctvtfhnwleivifvyqyhvlmllmvenmqvidyhfkslc*ypl
vhqnlkkhcvmvqkstihskrssrk---

---IRAIKMLLKLGIDSAASEFWNPKTSKYDMXFKNIGSSHLKTSLNSGEQLMSRYLEIL
KKYPIAFFEDPFGEDDWENHGKITAAIGNKIQIIGDDLLCTNPERIKKAISEKTVNSLLL
KINQIGTLTETIEAAKLAKEAGWGCLVSHRSGETDDHFIADLVVGXWYW*nkxlvhlvdl
kdyqniid**klkknlkns*ikw*fi*vcw*xsfxhfkkikkxk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFF763 (AFF763Q) /CSM/AF/AFF7-C/AFF763Q.Seq.d/                       2434   0.0
CFC343 (CFC343Q) /CSM/CF/CFC3-B/CFC343Q.Seq.d/                       1205   0.0
AFO218 (AFO218Q) /CSM/AF/AFO2-A/AFO218Q.Seq.d/                       1205   0.0
CFJ407 (CFJ407Q) /CSM/CF/CFJ4-A/CFJ407Q.Seq.d/                       1203   0.0
SFJ731 (SFJ731Q) /CSM/SF/SFJ7-B/SFJ731Q.Seq.d/                       1201   0.0
CFG491 (CFG491Q) /CSM/CF/CFG4-D/CFG491Q.Seq.d/                       1199   0.0
CFG261 (CFG261Q) /CSM/CF/CFG2-C/CFG261Q.Seq.d/                       1199   0.0
SFK174 (SFK174Q) /CSM/SF/SFK1-D/SFK174Q.Seq.d/                       1197   0.0
AFB731 (AFB731Q) /CSM/AF/AFB7-B/AFB731Q.Seq.d/                       1191   0.0
CFH624 (CFH624Q) /CSM/CF/CFH6-A/CFH624Q.Seq.d/                       1174   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U09736|U09736.1 Entamoeba histolytica HM1:IMSS enolase (enl-1) mRNA, complete cds.    56   6e-25  5
BD183626|BD183626.1 Material for diagnosing malaria and immune antigen for suppressing growth of plasmodium.    58   8e-19  6
BD183625|BD183625.1 Material for diagnosing malaria and immune antigen for suppressing growth of plasmodium.    58   8e-19  6
U00152|U00152.1 Plasmodium falciparum enolase gene complete cds.    58   8e-19  6
AB026051|AB026051.1 Plasmodium falciparum mRNA for enolase, complete cds.    58   8e-19  6
BM162118|BM162118.1 EST564641 PyBS Plasmodium yoelii yoelii cDNA clone PYCKN41 5' end, mRNA sequence.    70   5e-18  3
BM165201|BM165201.1 EST567724 PyBS Plasmodium yoelii yoelii cDNA clone PYCMI87 5' end, mRNA sequence.    70   6e-18  3
BM164058|BM164058.1 EST566581 PyBS Plasmodium yoelii yoelii cDNA clone PYCLP21 5' end, mRNA sequence.    70   7e-16  2
BM166362|BM166362.1 EST568885 PyBS Plasmodium yoelii yoelii cDNA clone PYCNG69 5' end, mRNA sequence.    70   1e-15  2
AE006265|AE006265.1 Lactococcus lactis subsp. lactis IL1403 section 27 of 218 of the complete genome.    44   3e-14  8

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q9U615) RecName: Full=Enolase;          EC=4.2.1.11; AltName: F...   385   e-110
DQ497988_1(DQ497988|pid:none) Polypterus senegalus enolase B mRN...   382   e-110
(Q27727) RecName: Full=Enolase;          EC=4.2.1.11; AltName: F...   386   e-109
AM910990_161(AM910990|pid:none) Plasmodium knowlesi strain H chr...   384   e-109
(P17183) RecName: Full=Gamma-enolase;          EC=4.2.1.11; AltN...   378   e-108
AK290525_1(AK290525|pid:none) Homo sapiens cDNA FLJ77877 complet...   377   e-108
(P09104) RecName: Full=Gamma-enolase;          EC=4.2.1.11; AltN...   377   e-108
JC1039(JC1039)phosphopyruvate hydratase (EC 4.2.1.11) - rat           377   e-108
DQ497985_1(DQ497985|pid:none) Polypterus senegalus enolase A mRN...   378   e-108
(P07323) RecName: Full=Gamma-enolase;          EC=4.2.1.11; AltN...   377   e-108

 psg: 0.56  gvh: 0.44  alm: 0.38  top: 0.53  tms: 0.00  mit: 0.29  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: nuclear
	 28.0 %: cytoplasmic
	 16.0 %: mitochondrial
	  8.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for AFF763 is nuc

>AFF763F.Seq
AATATTTTAATAATTATAATTTTAAAAAATGAGTATTGAATCAATTAAAGCTAGAGAAAT
TTTGGATAGTAGAGGTAATCCAACATTAGAAGTTGAAGTATGGACAAGTGAAGGAATGTT
TTCAGCCAAAGTACCAAGTGGAGCATCAACTGGTATTCATGAAGCATTTGAATTAAGAGA
TGGTGATAAAAGTCGTTATTTTGGATATGGTGTTTTGAATGCAATTGTTAATGTAAATAA
TTTAATATCACCAACATTGAAAGGTATGAGAGTTGATAATCAAAAAGCAATAGATGATAA
AATGATTGAATTGGATGGTACAAAGAATAAATCAAAACTTGGGTCCAATGCAATCGTTGG
TGTATCAATGGCAGTGGCAAGAGCAGCCGCTTCTATTAAAGGTGTGCCCTTGTACCGTTA
CATTTCACAATTGGCTGGAAATAGTAATATTCGTTTACCAGTACCATGTTTTAATGTTAT
TAATGGTGGAAAACATGCAGGTAATAGACTACCATTTCAAGAGTTTATGCTAGTACCCAT
TGGTGCACCAAAATTTAAAGAAGCATTGCGTTATGGTTCAGAAGTCTACCATTCACTCAA
AGAGATCATCAAGGAAAGA----------

>AFF763Z.Seq
----------TCATTCGGGCTATCAAGATGTTATTAAAATTGGGTATTGATTCTGCTGCT
TCAGAATTTTGGAATCCAAAGACTTCAAAATATGATATGGANTTCAAGAATATAGGATCC
TCACATTTGAAGACTAGTTTAAATAGTGGTGAGCAATTAATGTCTAGATATTTAGAGATT
TTAAAAAAGTATCCAATTGCATTCTTTGAAGATCCATTTGGTGAAGATGATTGGGAAAAT
CATGGTAAAATTACAGCTGCAATTGGTAATAAAATTCAAATCATTGGTGATGATTTACTT
TGTACAAATCCAGAAAGAATTAAAAAAGCAATTTCTGAGAAAACTGTAAATTCATTATTA
TTGAAAATAAATCAAATCGGTACACTAACTGAAACTATTGAAGCTGCAAAACTTGCAAAA
GAAGCTGGTTGGGGTTGTTTAGTTAGTCATCGTTCAGGTGAAACTGATGATCATTTCATT
GCTGATCTTGTAGTTGGTNCTTGGTACTGGTGAAATAAAATNCTGGTGCACCTTGTAGAT
TTGAAAGATTATCAAAATATAATAGATTAATGAAAATTGAAGAAGAACCTGAAAAACTCA
TAGATCAAATGGTAATTCATTTGAGTATGCTGGTGAAGNAGTTTTATNCATTTTAAAAAA
ATAAAAAAAANNAAAAA

>AFF763P.Seq
AATATTTTAATAATTATAATTTTAAAAAATGAGTATTGAATCAATTAAAGCTAGAGAAAT
TTTGGATAGTAGAGGTAATCCAACATTAGAAGTTGAAGTATGGACAAGTGAAGGAATGTT
TTCAGCCAAAGTACCAAGTGGAGCATCAACTGGTATTCATGAAGCATTTGAATTAAGAGA
TGGTGATAAAAGTCGTTATTTTGGATATGGTGTTTTGAATGCAATTGTTAATGTAAATAA
TTTAATATCACCAACATTGAAAGGTATGAGAGTTGATAATCAAAAAGCAATAGATGATAA
AATGATTGAATTGGATGGTACAAAGAATAAATCAAAACTTGGGTCCAATGCAATCGTTGG
TGTATCAATGGCAGTGGCAAGAGCAGCCGCTTCTATTAAAGGTGTGCCCTTGTACCGTTA
CATTTCACAATTGGCTGGAAATAGTAATATTCGTTTACCAGTACCATGTTTTAATGTTAT
TAATGGTGGAAAACATGCAGGTAATAGACTACCATTTCAAGAGTTTATGCTAGTACCCAT
TGGTGCACCAAAATTTAAAGAAGCATTGCGTTATGGTTCAGAAGTCTACCATTCACTCAA
AGAGATCATCAAGGAAAGA----------TCATTCGGGCTATCAAGATGTTATTAAAATT
GGGTATTGATTCTGCTGCTTCAGAATTTTGGAATCCAAAGACTTCAAAATATGATATGGA
NTTCAAGAATATAGGATCCTCACATTTGAAGACTAGTTTAAATAGTGGTGAGCAATTAAT
GTCTAGATATTTAGAGATTTTAAAAAAGTATCCAATTGCATTCTTTGAAGATCCATTTGG
TGAAGATGATTGGGAAAATCATGGTAAAATTACAGCTGCAATTGGTAATAAAATTCAAAT
CATTGGTGATGATTTACTTTGTACAAATCCAGAAAGAATTAAAAAAGCAATTTCTGAGAA
AACTGTAAATTCATTATTATTGAAAATAAATCAAATCGGTACACTAACTGAAACTATTGA
AGCTGCAAAACTTGCAAAAGAAGCTGGTTGGGGTTGTTTAGTTAGTCATCGTTCAGGTGA
AACTGATGATCATTTCATTGCTGATCTTGTAGTTGGTNCTTGGTACTGGTGAAATAAAAT
NCTGGTGCACCTTGTAGATTTGAAAGATTATCAAAATATAATAGATTAATGAAAATTGAA
GAAGAACCTGAAAAACTCATAGATCAAATGGTAATTCATTTGAGTATGCTGGTGAAGNAG
TTTTATNCATTTTAAAAAAATAAAAAAAANNAAAAA