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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFA594
Library AF
(Link to library)
Clone ID AFA594
Atlas ID -
NBRP ID
dictyBase ID
Link to Contig Contig-U14920-1
Original site URL
Representative seq. ID AFA594P
(Link to Original site)
Representative DNA sequence 
>AFA594 (AFA594Q) /CSM/AF/AFA5-D/AFA594Q.Seq.d/
ATTTTCTGNACAACAACTCTATTCGTTTATATAAAATGAACTCAGCATCAAAGTTATTTG
TTGTTTTGGCTTCACCAGCCAACCAACGTAATATGGCCACCTTAAAGGATCTTAAGATTC
GTTTAGGTACTGTTAAAACCATTTCTAAACTTACCAAAACTTTACATATGGTAGCTTCAT
CAAAATTACGTAGTGCAGAAAAGAAAGCAGAAGAATCAGGTCCATACAGTGTTGGTCCAC
AAAAAGTTTTAGGTGTAGTAGAAACTGCTGATGCATTCAACACCGCCNCTGAACCAATTG
AAGATAGATCAAATAAACAACTTTTAATTGCCATCACCACCGATACTGGTATGTGTGGTC
CAGTTAATCXXXXXXXXXXTTNATAAATGGTCGTGATTTTGGTAAANGCANATTACAGTT
TCCCAGAAGTTTNACTTTTCTTAAATGAAATCATTAGCAAAGTACCAAATTATGACTCNG
CTCTCGTCGTTTACAACAAATTCAAAAATGCCCTCTCAGACAGTGTCGATCGTCAATTCA
TTCCAGGTTTCAATCTCCNCGAATTAANTCGTGATAAATTCTACGAATATACTACCTCTG
AAGATCGTGCTGCCACCATGAAAGATCTCTNTGAANTTTATTTAGCCTCATCCTTATGGA
CTGGTTTATATCAAAACAGAGCCTCTGAAANGGCTGCTCGTATGGTTGCTATGGATAACG
CTTCAAAGAATGGTGAATCTATTTCNCAAGCTCTCGGTCTCCAATATAACAG
sequence update 2001. 6. 1
Translated Amino Acid sequence 
FLXNNSIRLYKMNSASKLFVVLASPANQRNMATLKDLKIRLGTVKTISKLTKTLHMVASS
KLRSAEKKAEESGPYSVGPQKVLGVVETADAFNTAXEPIEDRSNKQLLIAITTDTGMCGP
VN---

---XINGRDFGKXXLQFPRSXTFLK*nh*qstkl*lxsrrlqqiqkcplrqcrssihsrf
qsprixs**ilriyyl*rscchhersl*xlfslilmdwfiskqsl*xgcsygcyg*rfke
w*iyfxssrspi*q
Translated Amino Acid sequence (All Frames) 
Frame A:
ifxtttlfvyik*tqhqsyllfwlhqptnviwpp*rilrfv*vllkpflnlpklyiw*lh
qnyvvqkrkqknqvhtvlvhkkf*v**kllmhstpplnqlkidqinnf*lpsppilvcvv
qli---

---XINGRDFGKXXLQFPRSXTFLK*nh*qstkl*lxsrrlqqiqkcplrqcrssihsrf
qsprixs**ilriyyl*rscchhersl*xlfslilmdwfiskqsl*xgcsygcyg*rfke
w*iyfxssrspi*q

Frame B:
fsxqqlysfi*nelsikviccfgftsqpt*yghlkgs*dsfryc*nhf*tyqnftygsfi
kit*crkesrrirsiqcwstksfrcsrnc*ciqhrx*tn*r*ik*ttfnchhhrywyvws
s*---

---x*mvvilvxaxysfpevxlflneiiskvpnydsalvvynkfknalsdsvdrqfipgf
nlxelxrdkfyeyttsedraatmkdlxexylasslwtglyqnrasexaarmvamdnaskn
gesisqalglqyn

Frame C:
FLXNNSIRLYKMNSASKLFVVLASPANQRNMATLKDLKIRLGTVKTISKLTKTLHMVASS
KLRSAEKKAEESGPYSVGPQKVLGVVETADAFNTAXEPIEDRSNKQLLIAITTDTGMCGP
VN---

---xkws*fw*xxitvsqkfxfs*mkslakyqimtxlssfttnskmpsqtvsivnsfqvs
isxn*xvinstnilplkivlpp*kisxxfi*phpyglvyikteplkxllvwllwitlqrm
vnlfxklsvsnit
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFA594 (AFA594Q) /CSM/AF/AFA5-D/AFA594Q.Seq.d/                       1394   0.0
AFA276 (AFA276Q) /CSM/AF/AFA2-D/AFA276Q.Seq.d/                        712   0.0
VFH751 (VFH751Q) /CSM/VF/VFH7-C/VFH751Q.Seq.d/                        708   0.0
VFG758 (VFG758Q) /CSM/VF/VFG7-C/VFG758Q.Seq.d/                        708   0.0
VSE705 (VSE705Q) /CSM/VS/VSE7-A/VSE705Q.Seq.d/                        696   0.0
FC-AH08 (FC-AH08Q) /CSM/FC/FC-AH/FC-AH08Q.Seq.d/                      696   0.0
AFD678 (AFD678Q) /CSM/AF/AFD6-D/AFD678Q.Seq.d/                        609   e-173
FC-IC0252 (FC-IC0252Q) /CSM/FC-IC/FC-IC0252Q.Seq.d/                   563   e-159
FC-IC1545 (FC-IC1545Q) /CSM/FC-IC/FC-IC1545Q.Seq.d/                   472   e-132
AFK127 (AFK127Q) /CSM/AF/AFK1-B/AFK127Q.Seq.d/                        450   e-125
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

BH278026|BH278026.1 CH230-166A15.TJB CHORI-230 Segment 1 Rattus norvegicus genomic clone CH230-166A15, DNA sequence.    46  0.64  1
AC128350|AC128350.3 Rattus norvegicus clone CH230-158N22, *** SEQUENCING IN PROGRESS ***, 2 unordered pieces.    46  0.64  1
AC008062|AC008062.3 Homo sapiens clone RP11-68L6, complete sequence.    44  2.5  1
AV321766|AV321766.1 Mus musculus 13 days embryo male testis cDNA, RIKEN full-length enriched library, clone:6030437F20, 3' end partial sequence.    44  2.5  1
AC025021|AC025021.4 Homo sapiens chromosome 3 clone RP11-284A21 map 3, WORKING DRAFT SEQUENCE, 15 unordered pieces.    44  2.5  1
AC078779|AC078779.10 Homo sapiens chromosome 3 clone RP11-248F18, WORKING DRAFT SEQUENCE, 10 unordered pieces.    44  2.5  1
AC103072|AC103072.4 Rattus norvegicus clone CH230-172I11, *** SEQUENCING IN PROGRESS ***, 11 unordered pieces.    40  6.7  3
AC104938|AC104938.7 Homo sapiens chromosome 15 clone CTD-2524L6 map 15, *** SEQUENCING IN PROGRESS ***, 9 ordered pieces.    42  9.9  1
AC084299|AC084299.1 Homo sapiens chromosome 7 clone RP11-374D24, WORKING DRAFT SEQUENCE, 19 unordered pieces.    42  9.9  1
AC128749|AC128749.3 Rattus norvegicus clone CH230-460O4, *** SEQUENCING IN PROGRESS ***, 1 ordered piece.    42  9.9  1
dna update 2003. 7.22
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54DF1) RecName: Full=ATP synthase subunit gamma, mitochondrial...   408   e-112
(P07227) RecName: Full=ATP synthase gamma chain; AltName: Full=A...    94   3e-18
(O01666) RecName: Full=ATP synthase subunit gamma, mitochondrial...    94   6e-18
BT043688_1(BT043688|pid:none) Salmo salar clone HM5_2007 ATP syn...    92   1e-17
FJ607867_1(FJ607867|pid:none) Oncorhynchus mykiss ATP synthase H...    92   1e-17
BT048313_1(BT048313|pid:none) Salmo salar clone ssal-rgb2-640-05...    92   1e-17
AF007261_35(AF007261|pid:none) Reclinomonas americana mitochondr...    92   2e-17
CU459003_1825(CU459003|pid:none) Magnetospirillum gryphiswaldens...    92   2e-17
AY588070_1(AY588070|pid:none) Homalodisca coagulata putative mit...    91   3e-17
DQ445536_1(DQ445536|pid:none) Graphocephala atropunctata isolate...    91   4e-17
protein update 2009. 3.26
PSORT 

 psg: 0.74  gvh: 0.62  alm: 0.46  top: 0.53  tms: 0.00  mit: 0.47  mip: 0.11
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: mitochondrial
	 28.0 %: nuclear
	 12.0 %: cytoplasmic
	  8.0 %: cytoskeletal

>> prediction for AFA594 is mit
5' end seq. ID AFA594F
5' end seq. 
>AFA594F.Seq
ATTTTCTGNACAACAACTCTATTCGTTTATATAAAATGAACTCAGCATCAAAGTTATTTG
TTGTTTTGGCTTCACCAGCCAACCAACGTAATATGGCCACCTTAAAGGATCTTAAGATTC
GTTTAGGTACTGTTAAAACCATTTCTAAACTTACCAAAACTTTACATATGGTAGCTTCAT
CAAAATTACGTAGTGCAGAAAAGAAAGCAGAAGAATCAGGTCCATACAGTGTTGGTCCAC
AAAAAGTTTTAGGTGTAGTAGAAACTGCTGATGCATTCAACACCGCCNCTGAACCAATTG
AAGATAGATCAAATAAACAACTTTTAATTGCCATCACCACCGATACTGGTATGTGTGGTC
CAGTTAATC----------
Length of 5' end seq. 369
3' end seq. ID AFA594Z
3' end seq. 
>AFA594Z.Seq
----------TTNATAAATGGTCGTGATTTTGGTAAANGCANATTACAGTTTCCCAGAAG
TTTNACTTTTCTTAAATGAAATCATTAGCAAAGTACCAAATTATGACTCNGCTCTCGTCG
TTTACAACAAATTCAAAAATGCCCTCTCAGACAGTGTCGATCGTCAATTCATTCCAGGTT
TCAATCTCCNCGAATTAANTCGTGATAAATTCTACGAATATACTACCTCTGAAGATCGTG
CTGCCACCATGAAAGATCTCTNTGAANTTTATTTAGCCTCATCCTTATGGACTGGTTTAT
ATCAAAACAGAGCCTCTGAAANGGCTGCTCGTATGGTTGCTATGGATAACGCTTCAAAGA
ATGGTGAATCTATTTCNCAAGCTCTCGGTCTCCAATATAACAG
Length of 3' end seq. 393
Connected seq. ID AFA594P
Connected seq. 
>AFA594P.Seq
ATTTTCTGNACAACAACTCTATTCGTTTATATAAAATGAACTCAGCATCAAAGTTATTTG
TTGTTTTGGCTTCACCAGCCAACCAACGTAATATGGCCACCTTAAAGGATCTTAAGATTC
GTTTAGGTACTGTTAAAACCATTTCTAAACTTACCAAAACTTTACATATGGTAGCTTCAT
CAAAATTACGTAGTGCAGAAAAGAAAGCAGAAGAATCAGGTCCATACAGTGTTGGTCCAC
AAAAAGTTTTAGGTGTAGTAGAAACTGCTGATGCATTCAACACCGCCNCTGAACCAATTG
AAGATAGATCAAATAAACAACTTTTAATTGCCATCACCACCGATACTGGTATGTGTGGTC
CAGTTAATC----------TTNATAAATGGTCGTGATTTTGGTAAANGCANATTACAGTT
TCCCAGAAGTTTNACTTTTCTTAAATGAAATCATTAGCAAAGTACCAAATTATGACTCNG
CTCTCGTCGTTTACAACAAATTCAAAAATGCCCTCTCAGACAGTGTCGATCGTCAATTCA
TTCCAGGTTTCAATCTCCNCGAATTAANTCGTGATAAATTCTACGAATATACTACCTCTG
AAGATCGTGCTGCCACCATGAAAGATCTCTNTGAANTTTATTTAGCCTCATCCTTATGGA
CTGGTTTATATCAAAACAGAGCCTCTGAAANGGCTGCTCGTATGGTTGCTATGGATAACG
CTTCAAAGAATGGTGAATCTATTTCNCAAGCTCTCGGTCTCCAATATAACAG
Length of connected seq. 762
Full length Seq ID -
Full length Seq. -
Length of full length seq. -