NBDC - アーカイブトップ - ヘルプ
データベースの説明 | データ項目の説明 | 利用許諾 | ヘルプ
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
Description of this DB | Description of data items | Download | License
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CHF627
Library CH
(Link to library)
Clone ID CHF627
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16507-1
Original site URL
Representative seq. ID CHF627P
(Link to Original site)
Representative DNA sequence 
>CHF627 (CHF627Q) /CSM/CH/CHF6-B/CHF627Q.Seq.d/
ACACACACATAAAAATGGTAATAAAAAAATTATTTATATTAATTTTTTGTTTGTTTTTAA
TTATCAATGAAATTAATGGAAAAAAAACAAAAATAAATGATATTAAAAAATCAAAACCAA
AACTATCATCAACATTATTAAATGTTCATATTGTTGCTCATACTCATGATGATGTTGGTT
GGTTAAAAACAGTTGATGAATATTATTATGGATCAAATATGTCAATTGCATTTGCAGGTG
TTCAATATACTTTGGATACAGCCATTACCTGTTTATTGGCAAGTCCAGAAAGAAAGTTTA
TTTATGTTGAAATTGCATTTTTCCAAAGATGGTGGGATGAACAAAGTACAACAATGCAAA
ATATTGTTAAGGGTTTGGTTGAAAGTGGTCAATTGGAATTCATAAATGGTGGGTATTGTA
TGAATGATGAGGCTACTACCTACTATGAXXXXXXXXXXTCNTAATCAATCATTACAATTC
ACCATTGTCACTGATAGAAGTCGTGGATGTGCATCGTTAAGAGATGGTCAATTGGATATG
ATGATGCATCGTCGTACTTTGAAAGATGATGGTCGTGGTGTTGGTCAACCAATGAATGAG
TCAACTCAAATCGTTACAACTAGTAAATTGATTTTCCATGATATTTCAAGTTATGCTCAA
TCTCATTATCGTCCTGCTGCACTCTCTTTATCACATCCATTACTCCCAATGTTTACAACC
ACTCAACAATCTTCAAACGATTGGAATTCTCAATATCAAGGTGTTTACTCACCATTGACT
TCAGCCTCACCTTTACCAAATGGTTTAAAGATTCAAACTCTACAATGGTTAGATAATCAA
GATAATACCATACTTTTACGTATTGAAAATATTTATCAAATTGATGGTCAAGATTCACAA
GATCCACAAACTATCACTTTAGATCTTTCAACTATCTTTTCAACAATTACAATCACTTCT
GCAACTGAAATGAATTTAACTGGTGTTCAAAAATTATCAAATTTATCAAGATTAAAATGG
AAAACTGTTGATGGTAAAAACTATGATCATAAATCATCATCATCAACTAAAGAAGATTCT
TCAAAGGTTTTGTTTTTACTTTTTCTCCAATGCAAATCAGAA
sequence update 2002.10.25
Translated Amino Acid sequence 
THIKMVIKKLFILIFCLFLIINEINGKKTKINDIKKSKPKLSSTLLNVHIVAHTHDDVGW
LKTVDEYYYGSNMSIAFAGVQYTLDTAITCLLASPERKFIYVEIAFFQRWWDEQSTTMQN
IVKGLVESGQLEFINGGYCMNDEATTYY---

---XNQSLQFTIVTDRSRGCASLRDGQLDMMMHRRTLKDDGRGVGQPMNESTQIVTTSKL
IFHDISSYAQSHYRPAALSLSHPLLPMFTTTQQSSNDWNSQYQGVYSPLTSASPLPNGLK
IQTLQWLDNQDNTILLRIENIYQIDGQDSQDPQTITLDLSTIFSTITITSATEMNLTGVQ
KLSNLSRLKWKTVDGKNYDHKSSSSTKEDSSKVLFLLFLQCKSE
Translated Amino Acid sequence (All Frames) 
Frame A:
tht*kw**knyly*ffvcf*lsmklmekkqk*milknqnqnyhqhy*mfilllilmmmlv
g*kqlmniimdqicqlhlqvfnilwiqplpvywqvqkeslfmlklhfskdggmnkvqqck
illrvwlkvvnwns*mvgiv*mmrllptm---

---s*siitihhch**kswmcivkrwsigyddassyfer*wswcwstne*vnsnryn**i
dfp*yfklcsislsscctlfitsitpnvynhstifkrlefsisrclltidfsltftkwfk
dsnstmvr*sr*yhtfty*kylsn*wsrftrstnyhfrsfnylfnnynhfcn*nefnwcs
kiikfikikmenc*w*kl*s*iiiin*rrffkgfvftfspmqir

Frame B:
hthkngnkkiiyinflfvfnyq*n*wkknknk*y*kiktktiiniikcsyccsys**cwl
vkns**illwikyvncicrcsiyfgyshylfigksrkkvylc*ncifpkmvg*tkynnak
yc*gfg*kwsigihkwwvlye**gyyll*---

---XNQSLQFTIVTDRSRGCASLRDGQLDMMMHRRTLKDDGRGVGQPMNESTQIVTTSKL
IFHDISSYAQSHYRPAALSLSHPLLPMFTTTQQSSNDWNSQYQGVYSPLTSASPLPNGLK
IQTLQWLDNQDNTILLRIENIYQIDGQDSQDPQTITLDLSTIFSTITITSATEMNLTGVQ
KLSNLSRLKWKTVDGKNYDHKSSSSTKEDSSKVLFLLFLQCKSE

Frame C:
THIKMVIKKLFILIFCLFLIINEINGKKTKINDIKKSKPKLSSTLLNVHIVAHTHDDVGW
LKTVDEYYYGSNMSIAFAGVQYTLDTAITCLLASPERKFIYVEIAFFQRWWDEQSTTMQN
IVKGLVESGQLEFINGGYCMNDEATTYY---

---xinhynsplslievvdvhr*emvnwi**civvl*kmmvvvlvnq*msqlkslqlvn*
fsmifqvmlnliivllhslyhihysqclqplnnlqtigilnikvfthh*lqphlyqmv*r
fklyng*iikiipyfyvlkifiklmvkihkihklsl*ifqlsfqqlqsllqlk*i*lvfk
nyqiyqd*ngkllmvktmiinhhhqlkkilqrfcfyffsnanq
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHQ387 (VHQ387Q) /CSM/VH/VHQ3-D/VHQ387Q.Seq.d/                       1310   0.0
VHQ167 (VHQ167Q) /CSM/VH/VHQ1-C/VHQ167Q.Seq.d/                       1310   0.0
VHP767 (VHP767Q) /CSM/VH/VHP7-C/VHP767Q.Seq.d/                       1310   0.0
VHP443 (VHP443Q) /CSM/VH/VHP4-B/VHP443Q.Seq.d/                       1310   0.0
VHO643 (VHO643Q) /CSM/VH/VHO6-B/VHO643Q.Seq.d/                       1310   0.0
VHK686 (VHK686Q) /CSM/VH/VHK6-D/VHK686Q.Seq.d/                       1310   0.0
VHJ468 (VHJ468Q) /CSM/VH/VHJ4-C/VHJ468Q.Seq.d/                       1310   0.0
VHI312 (VHI312Q) /CSM/VH/VHI3-A/VHI312Q.Seq.d/                       1310   0.0
VHI153 (VHI153Q) /CSM/VH/VHI1-C/VHI153Q.Seq.d/                       1310   0.0
VHH791 (VHH791Q) /CSM/VH/VHH7-D/VHH791Q.Seq.d/                       1310   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M82822|M82822.1 Dictyostelium discoideum alpha-mannosidase (manA) gene, complete cds.  1205   0.0    6
CK149800|CK149800.1 CsmgEST00336 Culicoides sonorensis female serum-fed midgut cDNA Library Culicoides sonorensis cDNA, mRNA sequence.   117   6e-24  3
CB073433|CB073433.1 taa32h01.y1 Hydra EST -II Hydra magnipapillata cDNA 5' similar to TR:Q93093 Q93093 TRUNCATED LYSOSOMAL ACID ALPHA-MANNOSIDASE ;, mRNA sequence.    52   1e-13  3
DN814237| mannosidase 2, alpha B1 [Mus musculus], mRNA sequence.    52   1e-13  3
DN816506|A Chain A, The Structure Of The Bovine Lysosomal A-Mannosidase Suggests A Novel Mechanism For Low Ph Activation, mRNA sequence.    52   1e-13  3
CV682907|CV682907.1 service43_H06_sjs2-16-2B11-T3 SJS Schistosoma japonicum cDNA, mRNA sequence.    74   2e-13  2
CX633815|CX633815.1 taj44a03.y3 Hydra EST UCI 5 Hydra magnipapillata cDNA 5' similar to TR:Q93093 Q93093 TRUNCATED LYSOSOMAL ACID ALPHA-MANNOSIDASE ;, mRNA sequence.    52   2e-13  3
DN816232| lysosomal alpha-mannosidase [Cavia porcellus], mRNA sequence.    52   2e-13  3
CV688215|CV688215.1 sjs2-16-2_G01_sjs2-16-2G01-T3 SJS Schistosoma japonicum cDNA, mRNA sequence.    74   2e-13  2
CV682854|CV682854.1 service30_G09_sjs2-14-10C09-T3 SJS Schistosoma japonicum cDNA, mRNA sequence.    74   2e-13  2
dna update 2005. 7.28
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P34098) RecName: Full=Lysosomal alpha-mannosidase;          Sho...   327   7e-90
M82822_1(M82822|pid:none) Dictyostelium discoideum alpha-mannosi...   323   6e-87
(Q29451) RecName: Full=Lysosomal alpha-mannosidase;          Sho...   147   8e-34
AK291572_1(AK291572|pid:none) Homo sapiens cDNA FLJ76867 complet...   145   2e-33
U05572_1(U05572|pid:none) Human lysosomal alpha-mannosidase (MAN...   145   2e-33
AE014134_1973(AE014134|pid:none) Drosophila melanogaster chromos...   145   2e-33
AB209921_1(AB209921|pid:none) Homo sapiens mRNA for mannosidase,...   145   2e-33
AY036155_1(AY036155|pid:none) Cavia porcellus lysosomal alpha-ma...   145   3e-33
(Q8VHC8) RecName: Full=Lysosomal alpha-mannosidase;          Sho...   145   3e-33
JC2200(JC2200)alpha-mannosidase (EC 3.2.1.24) precursor - human       144   5e-33
protein update 2009. 3.31
PSORT 

 psg: 1.01  gvh: 0.62  alm: 0.17  top: 0.43  tms: 0.07  mit: 0.32  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 32.0 %: endoplasmic reticulum
	 16.0 %: cytoplasmic
	 12.0 %: mitochondrial
	 12.0 %: Golgi
	 12.0 %: nuclear
	  8.0 %: plasma membrane
	  4.0 %: vesicles of secretory system
	  4.0 %: extracellular, including cell wall

>> prediction for CHF627 is end
5' end seq. ID CHF627F
5' end seq. 
>CHF627F.Seq
ACACACACATAAAAATGGTAATAAAAAAATTATTTATATTAATTTTTTGTTTGTTTTTAA
TTATCAATGAAATTAATGGAAAAAAAACAAAAATAAATGATATTAAAAAATCAAAACCAA
AACTATCATCAACATTATTAAATGTTCATATTGTTGCTCATACTCATGATGATGTTGGTT
GGTTAAAAACAGTTGATGAATATTATTATGGATCAAATATGTCAATTGCATTTGCAGGTG
TTCAATATACTTTGGATACAGCCATTACCTGTTTATTGGCAAGTCCAGAAAGAAAGTTTA
TTTATGTTGAAATTGCATTTTTCCAAAGATGGTGGGATGAACAAAGTACAACAATGCAAA
ATATTGTTAAGGGTTTGGTTGAAAGTGGTCAATTGGAATTCATAAATGGTGGGTATTGTA
TGAATGATGAGGCTACTACCTACTATGANNNNNNNNNN
Length of 5' end seq. 458
3' end seq. ID CHF627Z
3' end seq. 
>CHF627Z.Seq
NNNNNNNNNNTCNTAATCAATCATTACAATTCACCATTGTCACTGATAGAAGTCGTGGAT
GTGCATCGTTAAGAGATGGTCAATTGGATATGATGATGCATCGTCGTACTTTGAAAGATG
ATGGTCGTGGTGTTGGTCAACCAATGAATGAGTCAACTCAAATCGTTACAACTAGTAAAT
TGATTTTCCATGATATTTCAAGTTATGCTCAATCTCATTATCGTCCTGCTGCACTCTCTT
TATCACATCCATTACTCCCAATGTTTACAACCACTCAACAATCTTCAAACGATTGGAATT
CTCAATATCAAGGTGTTTACTCACCATTGACTTCAGCCTCACCTTTACCAAATGGTTTAA
AGATTCAAACTCTACAATGGTTAGATAATCAAGATAATACCATACTTTTACGTATTGAAA
ATATTTATCAAATTGATGGTCAAGATTCACAAGATCCACAAACTATCACTTTAGATCTTT
CAACTATCTTTTCAACAATTACAATCACTTCTGCAACTGAAATGAATTTAACTGGTGTTC
AAAAATTATCAAATTTATCAAGATTAAAATGGAAAACTGTTGATGGTAAAAACTATGATC
ATAAATCATCATCATCAACTAAAGAAGATTCTTCAAAGGTTTTGTTTTTACTTTTTCTCC
AATGCAAATCAGAA
Length of 3' end seq. 674
Connected seq. ID CHF627P
Connected seq. 
>CHF627P.Seq
ACACACACATAAAAATGGTAATAAAAAAATTATTTATATTAATTTTTTGTTTGTTTTTAA
TTATCAATGAAATTAATGGAAAAAAAACAAAAATAAATGATATTAAAAAATCAAAACCAA
AACTATCATCAACATTATTAAATGTTCATATTGTTGCTCATACTCATGATGATGTTGGTT
GGTTAAAAACAGTTGATGAATATTATTATGGATCAAATATGTCAATTGCATTTGCAGGTG
TTCAATATACTTTGGATACAGCCATTACCTGTTTATTGGCAAGTCCAGAAAGAAAGTTTA
TTTATGTTGAAATTGCATTTTTCCAAAGATGGTGGGATGAACAAAGTACAACAATGCAAA
ATATTGTTAAGGGTTTGGTTGAAAGTGGTCAATTGGAATTCATAAATGGTGGGTATTGTA
TGAATGATGAGGCTACTACCTACTATGA----------TCNTAATCAATCATTACAATTC
ACCATTGTCACTGATAGAAGTCGTGGATGTGCATCGTTAAGAGATGGTCAATTGGATATG
ATGATGCATCGTCGTACTTTGAAAGATGATGGTCGTGGTGTTGGTCAACCAATGAATGAG
TCAACTCAAATCGTTACAACTAGTAAATTGATTTTCCATGATATTTCAAGTTATGCTCAA
TCTCATTATCGTCCTGCTGCACTCTCTTTATCACATCCATTACTCCCAATGTTTACAACC
ACTCAACAATCTTCAAACGATTGGAATTCTCAATATCAAGGTGTTTACTCACCATTGACT
TCAGCCTCACCTTTACCAAATGGTTTAAAGATTCAAACTCTACAATGGTTAGATAATCAA
GATAATACCATACTTTTACGTATTGAAAATATTTATCAAATTGATGGTCAAGATTCACAA
GATCCACAAACTATCACTTTAGATCTTTCAACTATCTTTTCAACAATTACAATCACTTCT
GCAACTGAAATGAATTTAACTGGTGTTCAAAAATTATCAAATTTATCAAGATTAAAATGG
AAAACTGTTGATGGTAAAAACTATGATCATAAATCATCATCATCAACTAAAGAAGATTCT
TCAAAGGTTTTGTTTTTACTTTTTCTCCAATGCAAATCAGAA
Length of connected seq. 1112
Full length Seq ID -
Full length Seq. -
Length of full length seq. -