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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFF724
Library AF
(Link to library)
Clone ID AFF724
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID AFF724P
(Link to Original site)
Representative DNA sequence 
>AFF724 (AFF724Q) /CSM/AF/AFF7-A/AFF724Q.Seq.d/
ATACAATTTAATTTCATTAATTTTTTAATGGCAAAACTAAAAATAATTATTTATATTGTA
ATAATTTTTTGTAGTAGTATCCAATTTTCTTTTGGATACGAAAAGGCGATTGCTTCATTT
TATCCACCTCCAGGTGATTTGCATCCCACTACTCATAATTTTTGGGCATGTAATAGTGTC
ATTAACCAAAACGGTGGATTCGGTCCACTTGAATGCAATAATGCCGGTATCTATGCAATT
TTCTCGACCGCCAATGAGATCATCGACGGTAATGAATACATGCTTCCACTATTTTATATT
GGAAGATCCACTATAGATGTTCGAGATAGAATTGCTGATCACAGTAGACGAAACCATGTT
GATAATGATTTCATTGTGTGTTATATGCGTTTCCCAGCATTGGATGATTATGGTTACAGT
AACAAAGTAACACCCGTTGAACAAGCTCTCCTTCACCAATTCAAAACTCAATTCAACTCA
TGGGATAACCCTCGGTTTTGATCCACAGTATGATACTGCTTATCCAGATGAAAATGGTTT
GGGATGGGATATCCTTGCATCTTTTTATAATATGGATATCAATGCTCCATCTTTTAAXXX
XXXXXXXTGGTTATATTTCTCGTACTGCTAGTCGTGTTGGTAACTCTATACGTAGCAGAG
CTACTCGTTATGCTAATCAAGGTTACAATTCCATGAGAAATGGTGCACAAAGAGCTGGCA
ACGGCGCACGTAATTATGCTAATGAAGGTTACAATTCCGTGAGAGATGGTGCACAAAGAG
CTGGCAACGGCGCACGTAATTATGCTCAACAAGGTTATCAAGCATGGAGAGAAGGTGTCA
ATGAGATTGGTGATGATATCGCCAATACTGCACATGATGTAGGTGATGGTTTCGCCAATA
CTGCACGTGAAGTAGGTGATGGAATTAGAATCACTGCAGAGGAGGGATATGAACTCATTA
AAGATGGAGCTGAAGAAACTTGGGTGAAGAAATATTAAAGGTTATCCTTAATTAAAATTA
AAAAAGCACAACAAAGAATGGCTGAACAAGAAGAAAGAAGAAGGTTGATGCAAGAAGAAA
GTGAAAGAAGACGATTGCAAAGAAACAGAGAAAGACAAGAGCCAATTAGAACTTATACAA
CTGCACAAGAAGCNCAACGATATATACCCAAACCTGGTA
sequence update 2001. 6. 2
Translated Amino Acid sequence 
ynlislif*wqn*k*lfil**ffvvvsnflldtkrrllhfihlqviciplliifghvivs
ltktvdsvhlnaimpvsmqfsrppmrsstvmntcfhyfiledpl*mfeiellitvdetml
imislcvicvsqhwmimvtvtk*HPLNKLSFTNSKLNSTHGITLGFDPQYDTAYPDENGL
GWDILASFYNMDINAPSF---

---GYISRTASRVGNSIRSRATRYANQGYNSMRNGAQRAGNGARNYANEGYNSVRDGAQR
AGNGARNYAQQGYQAWREGVNEIGDDIANTAHDVGDGFANTAREVGDGIRITAEEGYELI
KDGAEETWVKKY*rlslikikkaqqrmaeqeerrrlmqeeserrrlqrnrerqepirtyt
taqeaqryipkpg
Translated Amino Acid sequence (All Frames) 
Frame A:
iqfnfinflmaklkiiiyiviifcssiqfsfgyekaiasfypppgdlhptthnfwacnsv
inqnggfgplecnnagiyaifstaneiidgneymlplfyigrstidvrdriadhsrrnhv
dndfivcymrfpalddygysnkvtpveqallhqfktqfnswdnprf*stv*yclsr*kwf
gmgypcifl*ygyqcsif*---

---wlyfsyc*scw*lyt*qsyslc*srlqfhekwctkswqrrt*lc**rlqfrerwctk
swqrrt*lcstrlssmerrcq*dw**yrqyct*cr*wfrqyct*sr*wn*nhcrggi*th
*rws*rnlgeeilkviln*n*ksttkng*trrkkkvdarrk*kktiakkqrktran*nly
nctrsxtiytqtw

Frame B:
ynlislif*wqn*k*lfil**ffvvvsnflldtkrrllhfihlqviciplliifghvivs
ltktvdsvhlnaimpvsmqfsrppmrsstvmntcfhyfiledpl*mfeiellitvdetml
imislcvicvsqhwmimvtvtk*HPLNKLSFTNSKLNSTHGITLGFDPQYDTAYPDENGL
GWDILASFYNMDINAPSF---

---GYISRTASRVGNSIRSRATRYANQGYNSMRNGAQRAGNGARNYANEGYNSVRDGAQR
AGNGARNYAQQGYQAWREGVNEIGDDIANTAHDVGDGFANTAREVGDGIRITAEEGYELI
KDGAEETWVKKY*rlslikikkaqqrmaeqeerrrlmqeeserrrlqrnrerqepirtyt
taqeaqryipkpg

Frame C:
ti*fh*ffngktknnylycnnfl**ypiffwirkgdcfilstsr*fashys*flgm**ch
*pkrwirst*mq*crylcnfldrq*dhrr**ihastilywkihyrcsr*nc*sq*tkpc*
**fhcvlyafpsig*lwlq*qsntr*tsspspiqnsiqlmg*psvlihsmilliqmkmvw
dgislhlfiiwismlhll---

---viflvllvvlvtlyvaellvmlikvtip*emvhkelatahvimlmkvtip*emvhke
latahvimlnkvikhgekvsmrlvmispilhmm*vmvspilhvk*vmeleslqrrdmnsl
kmelkklg*rnikgyp*lklkkhnkewlnkkkeeg*ckkkvkeddcketekdksqleliq
lhkkxndiypnlv
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFK831 (SFK831Q) /CSM/SF/SFK8-B/SFK831Q.Seq.d/                       1183   0.0
SFK165 (SFK165Q) /CSM/SF/SFK1-C/SFK165Q.Seq.d/                       1183   0.0
SFK115 (SFK115Q) /CSM/SF/SFK1-A/SFK115Q.Seq.d/                       1183   0.0
SFJ550 (SFJ550Q) /CSM/SF/SFJ5-C/SFJ550Q.Seq.d/                       1183   0.0
SFJ191 (SFJ191Q) /CSM/SF/SFJ1-D/SFJ191Q.Seq.d/                       1183   0.0
SFG762 (SFG762Q) /CSM/SF/SFG7-C/SFG762Q.Seq.d/                       1183   0.0
SFG737 (SFG737Q) /CSM/SF/SFG7-B/SFG737Q.Seq.d/                       1183   0.0
SFG642 (SFG642Q) /CSM/SF/SFG6-B/SFG642Q.Seq.d/                       1183   0.0
SFE450 (SFE450Q) /CSM/SF/SFE4-C/SFE450Q.Seq.d/                       1183   0.0
SFD458 (SFD458Q) /CSM/SF/SFD4-C/SFD458Q.Seq.d/                       1183   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116551|AC116551.2 Dictyostelium discoideum chromosome 2 map complement(581327-427576) strain AX4, complete sequence.    88   3e-15  8
AC117081|AC117081.2 Dictyostelium discoideum chromosome 2 map 5862124-6045772 strain AX4, complete sequence.    56   3e-05  10
AF250284|AF250284.1 Amsacta moorei entomopoxvirus, complete genome.    34   4e-04  13
AC116924|AC116924.3 Dictyostelium discoideum chromosome 2 map 6357117-6445670 strain AX4, complete sequence.    40   7e-04  7
AC116982|AC116982.2 Dictyostelium discoideum chromosome 2 map 3622643-3879522 strain AX4, complete sequence.    38   0.001  12
AC116960|AC116960.2 Dictyostelium discoideum chromosome 2 map complement(1004496-821614) strain AX4, complete sequence.    40   0.001  13
AL035476|AL035476.7 Plasmodium falciparum MAL4P3.                  36   0.002  12
AL049181|AL049181.4 Plasmodium falciparum DNA *** SEQUENCING IN PROGRESS *** from  MAL13P4.    38   0.003  13
AC117176|AC117176.2 Dictyostelium discoideum chromosome 2 map 5018074-5200947 strain AX4, complete sequence.    34   0.016  11
AC116979|AC116979.3 Dictyostelium discoideum chromosome 2 map 6445720-6776760 strain AX4, complete sequence.    36   0.024  13
dna update 2004. 4.26
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AJ628940_1(AJ628940|pid:none) Pisum sativum mRNA for late embryo...    51   9e-05
Z69360_4(Z69360|pid:none) Caenorhabditis elegans Cosmid F25H8, c...    49   4e-04
AC146855_28(AC146855|pid:none) Medicago truncatula clone mth2-16...    49   4e-04
Z69360_3(Z69360|pid:none) Caenorhabditis elegans Cosmid F25H8, c...    49   4e-04
AM421808_498(AM421808|pid:none) Neisseria meningitidis serogroup...    49   4e-04
T21369(T21369)hypothetical protein F25H8.5a - Caenorhabditis ele...    47   0.001
AL844509_656(AL844509|pid:none) Plasmodium falciparum 3D7 chromo...    46   0.002
AY061535_1(AY061535|pid:none) Drosophila melanogaster LP01629 fu...    45   0.005
BT016018_1(BT016018|pid:none) Drosophila melanogaster RE19971 fu...    45   0.005
AE014297_4758(AE014297|pid:none) Drosophila melanogaster chromos...    45   0.005
protein update 2009. 4.20
PSORT 

 psg: 0.73  gvh: 0.40  alm: 0.54  top: 0.53  tms: 0.00  mit: 0.34  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: nuclear
	 32.0 %: cytoplasmic
	 20.0 %: mitochondrial
	  8.0 %: vacuolar

>> prediction for AFF724 is nuc
5' end seq. ID AFF724F
5' end seq. 
>AFF724F.Seq
ATACAATTTAATTTCATTAATTTTTTAATGGCAAAACTAAAAATAATTATTTATATTGTA
ATAATTTTTTGTAGTAGTATCCAATTTTCTTTTGGATACGAAAAGGCGATTGCTTCATTT
TATCCACCTCCAGGTGATTTGCATCCCACTACTCATAATTTTTGGGCATGTAATAGTGTC
ATTAACCAAAACGGTGGATTCGGTCCACTTGAATGCAATAATGCCGGTATCTATGCAATT
TTCTCGACCGCCAATGAGATCATCGACGGTAATGAATACATGCTTCCACTATTTTATATT
GGAAGATCCACTATAGATGTTCGAGATAGAATTGCTGATCACAGTAGACGAAACCATGTT
GATAATGATTTCATTGTGTGTTATATGCGTTTCCCAGCATTGGATGATTATGGTTACAGT
AACAAAGTAACACCCGTTGAACAAGCTCTCCTTCACCAATTCAAAACTCAATTCAACTCA
TGGGATAACCCTCGGTTTTGATCCACAGTATGATACTGCTTATCCAGATGAAAATGGTTT
GGGATGGGATATCCTTGCATCTTTTTATAATATGGATATCAATGCTCCATCTTTTAA---
-------
Length of 5' end seq. 597
3' end seq. ID AFF724Z
3' end seq. 
>AFF724Z.Seq
----------TGGTTATATTTCTCGTACTGCTAGTCGTGTTGGTAACTCTATACGTAGCA
GAGCTACTCGTTATGCTAATCAAGGTTACAATTCCATGAGAAATGGTGCACAAAGAGCTG
GCAACGGCGCACGTAATTATGCTAATGAAGGTTACAATTCCGTGAGAGATGGTGCACAAA
GAGCTGGCAACGGCGCACGTAATTATGCTCAACAAGGTTATCAAGCATGGAGAGAAGGTG
TCAATGAGATTGGTGATGATATCGCCAATACTGCACATGATGTAGGTGATGGTTTCGCCA
ATACTGCACGTGAAGTAGGTGATGGAATTAGAATCACTGCAGAGGAGGGATATGAACTCA
TTAAAGATGGAGCTGAAGAAACTTGGGTGAAGAAATATTAAAGGTTATCCTTAATTAAAA
TTAAAAAAGCACAACAAAGAATGGCTGAACAAGAAGAAAGAAGAAGGTTGATGCAAGAAG
AAAGTGAAAGAAGACGATTGCAAAGAAACAGAGAAAGACAAGAGCCAATTAGAACTTATA
CAACTGCACAAGAAGCNCAACGATATATACCCAAACCTGGTA
Length of 3' end seq. 572
Connected seq. ID AFF724P
Connected seq. 
>AFF724P.Seq
ATACAATTTAATTTCATTAATTTTTTAATGGCAAAACTAAAAATAATTATTTATATTGTA
ATAATTTTTTGTAGTAGTATCCAATTTTCTTTTGGATACGAAAAGGCGATTGCTTCATTT
TATCCACCTCCAGGTGATTTGCATCCCACTACTCATAATTTTTGGGCATGTAATAGTGTC
ATTAACCAAAACGGTGGATTCGGTCCACTTGAATGCAATAATGCCGGTATCTATGCAATT
TTCTCGACCGCCAATGAGATCATCGACGGTAATGAATACATGCTTCCACTATTTTATATT
GGAAGATCCACTATAGATGTTCGAGATAGAATTGCTGATCACAGTAGACGAAACCATGTT
GATAATGATTTCATTGTGTGTTATATGCGTTTCCCAGCATTGGATGATTATGGTTACAGT
AACAAAGTAACACCCGTTGAACAAGCTCTCCTTCACCAATTCAAAACTCAATTCAACTCA
TGGGATAACCCTCGGTTTTGATCCACAGTATGATACTGCTTATCCAGATGAAAATGGTTT
GGGATGGGATATCCTTGCATCTTTTTATAATATGGATATCAATGCTCCATCTTTTAA---
-------TGGTTATATTTCTCGTACTGCTAGTCGTGTTGGTAACTCTATACGTAGCAGAG
CTACTCGTTATGCTAATCAAGGTTACAATTCCATGAGAAATGGTGCACAAAGAGCTGGCA
ACGGCGCACGTAATTATGCTAATGAAGGTTACAATTCCGTGAGAGATGGTGCACAAAGAG
CTGGCAACGGCGCACGTAATTATGCTCAACAAGGTTATCAAGCATGGAGAGAAGGTGTCA
ATGAGATTGGTGATGATATCGCCAATACTGCACATGATGTAGGTGATGGTTTCGCCAATA
CTGCACGTGAAGTAGGTGATGGAATTAGAATCACTGCAGAGGAGGGATATGAACTCATTA
AAGATGGAGCTGAAGAAACTTGGGTGAAGAAATATTAAAGGTTATCCTTAATTAAAATTA
AAAAAGCACAACAAAGAATGGCTGAACAAGAAGAAAGAAGAAGGTTGATGCAAGAAGAAA
GTGAAAGAAGACGATTGCAAAGAAACAGAGAAAGACAAGAGCCAATTAGAACTTATACAA
CTGCACAAGAAGCNCAACGATATATACCCAAACCTGGTA
Length of connected seq. 1169
Full length Seq ID -
Full length Seq. -
Length of full length seq. -