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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CHF552
Library CH
(Link to library)
Clone ID CHF552
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U11105-1
Original site URL
Representative seq. ID CHF552P
(Link to Original site)
Representative DNA sequence 
>CHF552 (CHF552Q) /CSM/CH/CHF5-C/CHF552Q.Seq.d/
ATAAGATTAAAATCATTGAAACTCAACAATTGGATACTTNTCAGAGGTTAGAAAATTGAA
ATTAGAAAGTACAAGTAGTGGAAATTTAATGGCAGGTCTTAATGGTACCTCTGGTAGACC
TTCATCATCTTCTCACTTTATTCCATCCTCTGTTTCTGCCGCTGCTAACAATATCAACAA
GAATGAAATCATGGAAGAGGTTAAAAAGGTAGAAGAGAAACTTCAAAAGAAAATTCGTGA
AGAGATTGATAATACAAAATCTGAACTCTCAAAGGTTGAACGTTCCGTTAAAGATAATCG
TAGTGAAATTGAAGGTTTGGAAAAAGATTGTAAGAATCAATTCGATAAACAAGACAATAA
GATCAAACAAGTTGAGGATGATTTGAAAAAGAGTGATTCATTACTTTTGTTAATGCAAAA
TAACCTCAAGAAATATAATGAATTTGTTGATAGAGAACGTGATCGTGAAAGTGAACGTTT
GAAACTTCAXXXXXXXXXXTCAATTGAAAGTATCGCTTGTAATAGTAATTACATTTTCAC
TTCATCACCTGATAACACCATCAAAGTTCATATCATTCGTAGTGGTAACACCAAATGTAT
AGAGACATTGGTTGGTCACACTGGTGAAGTTAATTGTGTCGTGGCCAATGAAAAATATCT
TTTCAGTTGTAGTTATGATAAAACTATCAAGGTTTGGGATTTGTCAACCTTTAAAGAAAT
TAAATCATTTGAGGGTGTTCATACAAAGTACATTAAAACATTGGCTTTGAGTGGACGTTA
TCTTTTTAGTGGTGGTAACGATCAAATCATTTACGTTTGGGATACTGAAACACTTAGTAT
GCTTTTCAATATGCAAGGTCATGAAGATTGGGTACTCTCTCTTCATTGTACCGCTAGTTA
TCTTTTCTCAACCTCAAAAGATAATGTCATCAAGATTTGGGATCTCTCAAATTTCAGTTG
TATCGATACTCTAAAAGGTCATTGGAATTCTGTCTCAAGTTGTGTCGTAAAAGATCGTTA
TCTATACAGTGGTTCTGAAGATAATTCAATCAAAGTTTGGGATCTCGATACACTTGAATG
TGTTTACACCATTCCAAAATCTCATTCTTTGGGTGTAAAATGTTTAATGGTTTTCAATAA
TCAAATCATTTCTGCTGCTTTCGAGGTTCAATTAAAGTTNGGAAGGCAATCGAAA
sequence update 2002.10.25
Translated Amino Acid sequence 
*d*nh*NSTIGYXSEVRKLKLESTSSGNLMAGLNGTSGRPSSSSHFIPSSVSAAANNINK
NEIMEEVKKVEEKLQKKIREEIDNTKSELSKVERSVKDNRSEIEGLEKDCKNQFDKQDNK
IKQVEDDLKKSDSLLLLMQNNLKKYNEFVDRERDRESERLKL---

---SIESIACNSNYIFTSSPDNTIKVHIIRSGNTKCIETLVGHTGEVNCVVANEKYLFSC
SYDKTIKVWDLSTFKEIKSFEGVHTKYIKTLALSGRYLFSGGNDQIIYVWDTETLSMLFN
MQGHEDWVLSLHCTASYLFSTSKDNVIKIWDLSNFSCIDTLKGHWNSVSSCVVKDRYLYS
GSEDNSIKVWDLDTLECVYTIPKSHSLGVKCLMVFNNQIISAAFEVQLKXGRQSK
Translated Amino Acid sequence (All Frames) 
Frame A:
irlkslklnnwilxrg*kieirkyk*wkfngrs*wylw*tfiifslysilcfcrc*qyqq
e*nhgrg*kgrretskens*rd**yki*tlkg*tfr*r*s**n*rfgkrl*esir*trq*
dqts*g*feke*fitfvnak*pqei**ic**rt*s*k*tfets---

---SIESIACNSNYIFTSSPDNTIKVHIIRSGNTKCIETLVGHTGEVNCVVANEKYLFSC
SYDKTIKVWDLSTFKEIKSFEGVHTKYIKTLALSGRYLFSGGNDQIIYVWDTETLSMLFN
MQGHEDWVLSLHCTASYLFSTSKDNVIKIWDLSNFSCIDTLKGHWNSVSSCVVKDRYLYS
GSEDNSIKVWDLDTLECVYTIPKSHSLGVKCLMVFNNQIISAAFEVQLKXGRQSK

Frame B:
*d*nh*NSTIGYXSEVRKLKLESTSSGNLMAGLNGTSGRPSSSSHFIPSSVSAAANNINK
NEIMEEVKKVEEKLQKKIREEIDNTKSELSKVERSVKDNRSEIEGLEKDCKNQFDKQDNK
IKQVEDDLKKSDSLLLLMQNNLKKYNEFVDRERDRESERLKL---

---qlkvslvivitfslhhlitpskfisfvvvtpnv*rhwlvtlvklivswpmknifsvv
vmiklsrfgicqplkklnhlrvfiqstlkhwl*vdviflvvvtiksftfgilkhlvcfsi
ckvmkigyslfivplvifsqpqkimssrfgisqisvvsil*kvigilsqvvs*kiviytv
vlkiiqskfgisihlnvftpfqnlilwv*nv*wfsiiksflllsrfn*sxegnr

Frame C:
kikiietqqldtxqrlen*n*kvqvvei*wqvlmvplvdlhhlltlfhplflplltistr
mkswkrlkr*krnfkrkfvkrliiqnlnsqrlnvplkiivvklkvwkkivrinsinktir
snklrmi*krvihyfc*ckitsrnimnllienvivkvnv*nf---

---n*kyrl***lhfhfit**hhqssyhs*w*hqmyrdigwshw*s*lcrgq*kisfql*
l**nyqglgfvnl*rn*ii*gcsykvh*nigfewtlsf*ww*rsnhlrlgy*nt*yafqy
ars*rlgtlsslyr*lsflnlkr*chqdlgslkfqlyryskrslefclklcrkrslsiqw
f*r*fnqslgsryt*mclhhskisffgckmfngfq*snhfccfrgsikvxkaie
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHF552 (CHF552Q) /CSM/CH/CHF5-C/CHF552Q.Seq.d/                       2298   0.0
VHK847 (VHK847Q) /CSM/VH/VHK8-B/VHK847Q.Seq.d/                       1374   0.0
SHC440 (SHC440Q) /CSM/SH/SHC4-B/SHC440Q.Seq.d/                       1374   0.0
AHN363 (AHN363Q) /CSM/AH/AHN3-C/AHN363Q.Seq.d/                       1374   0.0
AHA264 (AHA264Q) /CSM/AH/AHA2-C/AHA264Q.Seq.d/                       1372   0.0
VHJ702 (VHJ702Q) /CSM/VH/VHJ7-A/VHJ702Q.Seq.d/                       1370   0.0
VHC476 (VHC476Q) /CSM/VH/VHC4-D/VHC476Q.Seq.d/                       1360   0.0
VHA649 (VHA649Q) /CSM/VH/VHA6-C/VHA649Q.Seq.d/                       1354   0.0
VHJ626 (VHJ626Q) /CSM/VH/VHJ6-B/VHJ626Q.Seq.d/                       1352   0.0
VFE245 (VFE245Q) /CSM/VF/VFE2-B/VFE245Q.Seq.d/                       1352   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

BD140493|BD140493.1 Elongation factor-2 kinase (EF-2 kinase) and methods of usetherefor.  1318   0.0    2
AR193040|AR193040.1 Sequence 5 from patent US 6346406.           1318   0.0    2
U16856|U16856.1 Dictyostelium discoideum myosin heavy chain kinase A (MHCK A) mRNA, complete cds.  1318   0.0    2
BD140494|BD140494.1 Elongation factor-2 kinase (EF-2 kinase) and methods of use therefor.    58   3e-05  2
AR193041|AR193041.1 Sequence 7 from patent US 6346406.             58   3e-05  2
U90946|U90946.1 Dictyostelium discoideum myosin heavy chain kinase B (MHCK-B) mRNA, complete cds.    58   3e-05  2
AC101274|AC101274.1 Mus musculus clone RP23-101L24, LOW-PASS SEQUENCE SAMPLING.    44   0.001  3
CP000029|CP000029.1 Staphylococcus epidermidis RP62A, complete genome.    34   0.003  32
AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.    34   0.092  14
AR643125|AR643125.1 Sequence 7 from patent US 6864404.             50   0.093  1
dna update 2005. 7.23
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

A55532(A55532) myosin-heavy-chain kinase (EC 2.7.1.129) A - slim...   462   e-128
CP000492_814(CP000492|pid:none) Chlorobium phaeobacteroides DSM ...   148   3e-34
AX722159_1(AX722159|pid:none) Sequence 17 from Patent WO03024999...   146   1e-33
AK032174_1(AK032174|pid:none) Mus musculus adult male olfactory ...   146   1e-33
BC037320_1(BC037320|pid:none) Homo sapiens F-box and WD-40 domai...   146   1e-33
(Q969H0) RecName: Full=F-box/WD repeat-containing protein 7; Alt...   146   1e-33
AK223449_1(AK223449|pid:none) Homo sapiens mRNA for F-box protei...   146   1e-33
AF383178_1(AF383178|pid:none) Homo sapiens F-box protein FBX30 m...   146   1e-33
AL953881_1(AL953881|pid:none) Zebrafish DNA sequence from clone ...   146   2e-33
CR859650_1(CR859650|pid:none) Pongo abelii mRNA; cDNA DKFZp459C1...   145   3e-33
protein update 2009. 3.31
PSORT 

 psg: 0.57  gvh: 0.51  alm: 0.42  top: 0.53  tms: 0.00  mit: 0.22  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 28.0 %: nuclear
	 20.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for CHF552 is cyt
5' end seq. ID CHF552F
5' end seq. 
>CHF552F.Seq
ATAAGATTAAAATCATTGAAACTCAACAATTGGATACTTNTCAGAGGTTAGAAAATTGAA
ATTAGAAAGTACAAGTAGTGGAAATTTAATGGCAGGTCTTAATGGTACCTCTGGTAGACC
TTCATCATCTTCTCACTTTATTCCATCCTCTGTTTCTGCCGCTGCTAACAATATCAACAA
GAATGAAATCATGGAAGAGGTTAAAAAGGTAGAAGAGAAACTTCAAAAGAAAATTCGTGA
AGAGATTGATAATACAAAATCTGAACTCTCAAAGGTTGAACGTTCCGTTAAAGATAATCG
TAGTGAAATTGAAGGTTTGGAAAAAGATTGTAAGAATCAATTCGATAAACAAGACAATAA
GATCAAACAAGTTGAGGATGATTTGAAAAAGAGTGATTCATTACTTTTGTTAATGCAAAA
TAACCTCAAGAAATATAATGAATTTGTTGATAGAGAACGTGATCGTGAAAGTGAACGTTT
GAAACTTCANNNNNNNNNN
Length of 5' end seq. 499
3' end seq. ID CHF552Z
3' end seq. 
>CHF552Z.Seq
NNNNNNNNNNTCAATTGAAAGTATCGCTTGTAATAGTAATTACATTTTCACTTCATCACC
TGATAACACCATCAAAGTTCATATCATTCGTAGTGGTAACACCAAATGTATAGAGACATT
GGTTGGTCACACTGGTGAAGTTAATTGTGTCGTGGCCAATGAAAAATATCTTTTCAGTTG
TAGTTATGATAAAACTATCAAGGTTTGGGATTTGTCAACCTTTAAAGAAATTAAATCATT
TGAGGGTGTTCATACAAAGTACATTAAAACATTGGCTTTGAGTGGACGTTATCTTTTTAG
TGGTGGTAACGATCAAATCATTTACGTTTGGGATACTGAAACACTTAGTATGCTTTTCAA
TATGCAAGGTCATGAAGATTGGGTACTCTCTCTTCATTGTACCGCTAGTTATCTTTTCTC
AACCTCAAAAGATAATGTCATCAAGATTTGGGATCTCTCAAATTTCAGTTGTATCGATAC
TCTAAAAGGTCATTGGAATTCTGTCTCAAGTTGTGTCGTAAAAGATCGTTATCTATACAG
TGGTTCTGAAGATAATTCAATCAAAGTTTGGGATCTCGATACACTTGAATGTGTTTACAC
CATTCCAAAATCTCATTCTTTGGGTGTAAAATGTTTAATGGTTTTCAATAATCAAATCAT
TTCTGCTGCTTTCGAGGTTCAATTAAAGTTNGGAAGGCAATCGAAA
Length of 3' end seq. 706
Connected seq. ID CHF552P
Connected seq. 
>CHF552P.Seq
ATAAGATTAAAATCATTGAAACTCAACAATTGGATACTTNTCAGAGGTTAGAAAATTGAA
ATTAGAAAGTACAAGTAGTGGAAATTTAATGGCAGGTCTTAATGGTACCTCTGGTAGACC
TTCATCATCTTCTCACTTTATTCCATCCTCTGTTTCTGCCGCTGCTAACAATATCAACAA
GAATGAAATCATGGAAGAGGTTAAAAAGGTAGAAGAGAAACTTCAAAAGAAAATTCGTGA
AGAGATTGATAATACAAAATCTGAACTCTCAAAGGTTGAACGTTCCGTTAAAGATAATCG
TAGTGAAATTGAAGGTTTGGAAAAAGATTGTAAGAATCAATTCGATAAACAAGACAATAA
GATCAAACAAGTTGAGGATGATTTGAAAAAGAGTGATTCATTACTTTTGTTAATGCAAAA
TAACCTCAAGAAATATAATGAATTTGTTGATAGAGAACGTGATCGTGAAAGTGAACGTTT
GAAACTTCA----------TCAATTGAAAGTATCGCTTGTAATAGTAATTACATTTTCAC
TTCATCACCTGATAACACCATCAAAGTTCATATCATTCGTAGTGGTAACACCAAATGTAT
AGAGACATTGGTTGGTCACACTGGTGAAGTTAATTGTGTCGTGGCCAATGAAAAATATCT
TTTCAGTTGTAGTTATGATAAAACTATCAAGGTTTGGGATTTGTCAACCTTTAAAGAAAT
TAAATCATTTGAGGGTGTTCATACAAAGTACATTAAAACATTGGCTTTGAGTGGACGTTA
TCTTTTTAGTGGTGGTAACGATCAAATCATTTACGTTTGGGATACTGAAACACTTAGTAT
GCTTTTCAATATGCAAGGTCATGAAGATTGGGTACTCTCTCTTCATTGTACCGCTAGTTA
TCTTTTCTCAACCTCAAAAGATAATGTCATCAAGATTTGGGATCTCTCAAATTTCAGTTG
TATCGATACTCTAAAAGGTCATTGGAATTCTGTCTCAAGTTGTGTCGTAAAAGATCGTTA
TCTATACAGTGGTTCTGAAGATAATTCAATCAAAGTTTGGGATCTCGATACACTTGAATG
TGTTTACACCATTCCAAAATCTCATTCTTTGGGTGTAAAATGTTTAATGGTTTTCAATAA
TCAAATCATTTCTGCTGCTTTCGAGGTTCAATTAAAGTTNGGAAGGCAATCGAAA
Length of connected seq. 1185
Full length Seq ID -
Full length Seq. -
Length of full length seq. -