>CHF466 (CHF466Q) /CSM/CH/CHF4-C/CHF466Q.Seq.d/
TATAAAGATGGTAATAAACAAATTTCAACAGTTTATGGGCTAATTGAAAGATTATTTAAT
TTTGAAATGACATTATTCTTTAAATTAAAGATTGAAGAATCCGAATGGATGACTGGTGAA
TATTATGAAATTTCAACGGAATCAGTTGAAAATGAAGATTCCTCAAAAAATATAACATTT
GTAACGATTAGGGCAGATTCCGGTGTGAATTTAGCCATGGGTTTACAATATTATTTAAAG
TATTATTGCTTCTGTAGTTACACATGGTCCGGTGACCAATGTTCAATCACAAGTTATAGC
CAATTACCAGCGGTAACAGAGGGTAGTGTATCGATACCAGTGATATCAGCCTATAGATAT
TACATGAATGTGCGCACATTTGGGTACAGTACCACATGGTGGAATTGGAGTAGATGGGAG
CGTGAGATCGACTGGATGGCTTTGAACGGCTATAATCTACCATTAGCATTCGTTGGCCAG
GAGTATATCTGGTATCGTGTTTTCAGTGAGCTCGGTTTAAGCTTTGACCAAATAAGCACC
TGGTTAACAGGTCCAGCATTTTTACCATGGAATAGAATGGGTAATGTAAATGGCTGGXXX
XXXXXXXATTAACATTTGGCAATAATTCATTCTACAATCCAAATATACTCTACAATGCAT
GGAACGTCTTTTCAATGGTGGATGATGAATATGTAATTTCCACGGAAACATTCGAATTTG
ACATATCAGAATTCGCAATGCAATCTTTAAGTAACTACTTCATGGACCAATACTTCCTAT
TGATTGAAGCATTCAATGCCTCTGATGTGCAAACATTGTCAACTATTTCAATCGAGTTGT
TGGATATCATCAANTACATGGATGAAATTGCAAGTACGCAAAGTTCTCTTCAATTGGGGT
TATGGACATACCGTGGTAGATTATGGGCCTACCCAACCAACGATATCCCAACCCTTCAAA
ATAGTAGTAACTCAAATACCGCACCATACGAATTCAATGCTCGTAATGTCTTAACTTTAT
GGGGTCCTTCAAATTCTGTACTCCATGATTACGCTTTCAAACTATGGAGTGGTTTAGTAA
GTGATTTNTATTCACCACGTTGGCAACTCTTTTTAAAGTCATTAGTGCAATCTGTTGAAA
ATCGTAAACCATTCAATAAGGAATCATTCAATAGA

YKDGNKQISTVYGLIERLFNFEMTLFFKLKIEESEWMTGEYYEISTESVENEDSSKNITF
VTIRADSGVNLAMGLQYYLKYYCFCSYTWSGDQCSITSYSQLPAVTEGSVSIPVISAYRY
YMNVRTFGYSTTWWNWSRWEREIDWMALNGYNLPLAFVGQEYIWYRVFSELGLSFDQIST
WLTGPAFLPWNRMGNVNGW---

---LTFGNNSFYNPNILYNAWNVFSMVDDEYVISTETFEFDISEFAMQSLSNYFMDQYFL
LIEAFNASDVQTLSTISIELLDIIXYMDEIASTQSSLQLGLWTYRGRLWAYPTNDIPTLQ
NSSNSNTAPYEFNARNVLTLWGPSNSVLHDYAFKLWSGLVSDXYSPRWQLFLKSLVQSVE
NRKPFNKESFNR

Frame A:
YKDGNKQISTVYGLIERLFNFEMTLFFKLKIEESEWMTGEYYEISTESVENEDSSKNITF
VTIRADSGVNLAMGLQYYLKYYCFCSYTWSGDQCSITSYSQLPAVTEGSVSIPVISAYRY
YMNVRTFGYSTTWWNWSRWEREIDWMALNGYNLPLAFVGQEYIWYRVFSELGLSFDQIST
WLTGPAFLPWNRMGNVNGW---

---iniwq*filqskytlqcmerlfngg**icnfhgniri*hirirnaifk*llhgpilp
id*siqcl*canivnyfnrvvgyhqxhg*nckyakfssigvmdipw*imglpnqrypnps
k***lkyrtiriqcs*clnfmgsfkfctp*lrfqtmewfsk*fxfttlatlfkvisaic*
ks*tiq*giiq*

Frame B:
ikmvinkfqqfmg*lkdylilk*hysln*rlknpng*lvnimkfqrnqlkmkipqki*hl
*rlgqipv*i*pwvynii*siiasvvthgpvtnvqsqvianyqr*qrvvyryq*yqpidi
t*mcahlgtvphggigvdgsvrstgwl*taiiyh*hslarsisgivfsvssv*altk*ap
g*qvqhfyhgiewvm*ma---

---LTFGNNSFYNPNILYNAWNVFSMVDDEYVISTETFEFDISEFAMQSLSNYFMDQYFL
LIEAFNASDVQTLSTISIELLDIIXYMDEIASTQSSLQLGLWTYRGRLWAYPTNDIPTLQ
NSSNSNTAPYEFNARNVLTLWGPSNSVLHDYAFKLWSGLVSDXYSPRWQLFLKSLVQSVE
NRKPFNKESFNR

Frame C:
*rw**tnfnslwan*kii*f*ndiil*ikd*rirmddw*il*nfngis*k*rflkkynic
nd*grfrcefshgftilfkvllll*lhmvr*pmfnhkl*pitsgnrg*cidtsdisl*il
hecahiwvqyhmvele*mga*drldgferl*stisirwpgvylvscfq*arfkl*pnkhl
vnrssiftme*ng*ckwl---

---*hlaiihstiqiystmhgtsfqwwmmnm*fprkhsnltyqnsqcnl*vttswtntsy
*lkhsmplmckhcqlfqsscwissxtwmklqvrkvlfnwgyghtvvdygptqptisqpfk
ivvtqiphhtnsmlvms*lygvlqilysmitlsnygvv**vixihhvgnsf*sh*cnllk
ivnhsirnhsi

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHF466 (CHF466Q) /CSM/CH/CHF4-C/CHF466Q.Seq.d/                       2258   0.0
SLH504 (SLH504Q) /CSM/SL/SLH5-A/SLH504Q.Seq.d/                       1098   0.0
SHD225 (SHD225Q) /CSM/SH/SHD2-B/SHD225Q.Seq.d/                       1098   0.0
CHS129 (CHS129Q) /CSM/CH/CHS1-B/CHS129Q.Seq.d/                       1098   0.0
CHR606 (CHR606Q) /CSM/CH/CHR6-A/CHR606Q.Seq.d/                       1098   0.0
CHQ605 (CHQ605Q) /CSM/CH/CHQ6-A/CHQ605Q.Seq.d/                       1098   0.0
CFF471 (CFF471Q) /CSM/CF/CFF4-C/CFF471Q.Seq.d/                       1098   0.0
AHK591 (AHK591Q) /CSM/AH/AHK5-D/AHK591Q.Seq.d/                       1098   0.0
AHH868 (AHH868Q) /CSM/AH/AHH8-C/AHH868Q.Seq.d/                       1098   0.0
AHF813 (AHF813Q) /CSM/AH/AHF8-A/AHF813Q.Seq.d/                       1098   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

G65042|G65042.1 DH0622 Dictyostelium discoideum ( P.Dear ) Dictyostelium discoideum STS genomic 5', sequence tagged site.   299   e-144  2
AC129002|AC129002.6 Rattus norvegicus clone CH230-6M17, WORKING DRAFT SEQUENCE, 6 unordered pieces.    54   0.005  1
CR672702|CR672702.1 Tetraodon nigroviridis full-length cDNA.       50   0.009  2
AC131698|AC131698.3 Mus musculus chromosome 3 clone RP23-428E12, WORKING DRAFT SEQUENCE, 4 unordered pieces.    50   0.083  1
BX294385|BX294385.17 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone CH211-197E8.    50   0.083  1
CL345401|CL345401.1 RPCI44_280E9.r RPCI-44 Sus scrofa genomic clone RPCI44_280E9, genomic survey sequence.    34   0.16   3
AC079797|AC079797.4 Homo sapiens chromosome RPCI-11 clone RP11-633E4, WORKING DRAFT SEQUENCE, 5 unordered pieces.    40   0.24   6
AC137597|AC137597.2 Genomic sequence for Oryza sativa, Nipponbare strain, clone OSJNBa0022F22, from chromosome 3, complete sequence.    38   0.41   6
AL354741|AL354741.16 Human DNA sequence from clone RP11-49A19 on chromosome 13.    40   0.62   5
CR383670|CR383670.2 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone BUSM1-166E23.    40   0.72   4

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P54802) RecName: Full=Alpha-N-acetylglucosaminidase;          E...   148   4e-34
BX255926_1(BX255926|pid:none) Mouse DNA sequence from clone RP23...   147   7e-34
AF331669_1(AF331669|pid:none) Dromaius novaehollandiae lysosomal...   145   3e-33
AE015928_438(AE015928|pid:none) Bacteroides thetaiotaomicron VPI...   142   3e-32
AM456444_1(AM456444|pid:none) Vitis vinifera contig VV78X217584....   140   1e-31
AL442007_15(AL442007|pid:none) Oryza sativa genomic DNA, chromos...   137   7e-31
Y18209_1(Y18209|pid:none) Nicotiana tabacum mRNA for alpha-N-ace...   135   3e-30
AE015928_3588(AE015928|pid:none) Bacteroides thetaiotaomicron VP...   132   2e-29
CP000685_3107(CP000685|pid:none) Flavobacterium johnsoniae UW101...   128   3e-28
AP006841_677(AP006841|pid:none) Bacteroides fragilis YCH46 DNA, ...   127   7e-28

 psg: 0.90  gvh: 0.22  alm: 0.43  top: 0.47  tms: 0.00  mit: 0.23  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 36.0 %: nuclear
	  8.0 %: cytoskeletal
	  4.0 %: Golgi
	  4.0 %: vacuolar
	  4.0 %: mitochondrial
	  4.0 %: plasma membrane

>> prediction for CHF466 is cyt

>CHF466F.Seq
TATAAAGATGGTAATAAACAAATTTCAACAGTTTATGGGCTAATTGAAAGATTATTTAAT
TTTGAAATGACATTATTCTTTAAATTAAAGATTGAAGAATCCGAATGGATGACTGGTGAA
TATTATGAAATTTCAACGGAATCAGTTGAAAATGAAGATTCCTCAAAAAATATAACATTT
GTAACGATTAGGGCAGATTCCGGTGTGAATTTAGCCATGGGTTTACAATATTATTTAAAG
TATTATTGCTTCTGTAGTTACACATGGTCCGGTGACCAATGTTCAATCACAAGTTATAGC
CAATTACCAGCGGTAACAGAGGGTAGTGTATCGATACCAGTGATATCAGCCTATAGATAT
TACATGAATGTGCGCACATTTGGGTACAGTACCACATGGTGGAATTGGAGTAGATGGGAG
CGTGAGATCGACTGGATGGCTTTGAACGGCTATAATCTACCATTAGCATTCGTTGGCCAG
GAGTATATCTGGTATCGTGTTTTCAGTGAGCTCGGTTTAAGCTTTGACCAAATAAGCACC
TGGTTAACAGGTCCAGCATTTTTACCATGGAATAGAATGGGTAATGTAAATGGCTGGNNN
NNNNNNN

>CHF466Z.Seq
NNNNNNNNNNATTAACATTTGGCAATAATTCATTCTACAATCCAAATATACTCTACAATG
CATGGAACGTCTTTTCAATGGTGGATGATGAATATGTAATTTCCACGGAAACATTCGAAT
TTGACATATCAGAATTCGCAATGCAATCTTTAAGTAACTACTTCATGGACCAATACTTCC
TATTGATTGAAGCATTCAATGCCTCTGATGTGCAAACATTGTCAACTATTTCAATCGAGT
TGTTGGATATCATCAANTACATGGATGAAATTGCAAGTACGCAAAGTTCTCTTCAATTGG
GGTTATGGACATACCGTGGTAGATTATGGGCCTACCCAACCAACGATATCCCAACCCTTC
AAAATAGTAGTAACTCAAATACCGCACCATACGAATTCAATGCTCGTAATGTCTTAACTT
TATGGGGTCCTTCAAATTCTGTACTCCATGATTACGCTTTCAAACTATGGAGTGGTTTAG
TAAGTGATTTNTATTCACCACGTTGGCAACTCTTTTTAAAGTCATTAGTGCAATCTGTTG
AAAATCGTAAACCATTCAATAAGGAATCATTCAATAGA

>CHF466P.Seq
TATAAAGATGGTAATAAACAAATTTCAACAGTTTATGGGCTAATTGAAAGATTATTTAAT
TTTGAAATGACATTATTCTTTAAATTAAAGATTGAAGAATCCGAATGGATGACTGGTGAA
TATTATGAAATTTCAACGGAATCAGTTGAAAATGAAGATTCCTCAAAAAATATAACATTT
GTAACGATTAGGGCAGATTCCGGTGTGAATTTAGCCATGGGTTTACAATATTATTTAAAG
TATTATTGCTTCTGTAGTTACACATGGTCCGGTGACCAATGTTCAATCACAAGTTATAGC
CAATTACCAGCGGTAACAGAGGGTAGTGTATCGATACCAGTGATATCAGCCTATAGATAT
TACATGAATGTGCGCACATTTGGGTACAGTACCACATGGTGGAATTGGAGTAGATGGGAG
CGTGAGATCGACTGGATGGCTTTGAACGGCTATAATCTACCATTAGCATTCGTTGGCCAG
GAGTATATCTGGTATCGTGTTTTCAGTGAGCTCGGTTTAAGCTTTGACCAAATAAGCACC
TGGTTAACAGGTCCAGCATTTTTACCATGGAATAGAATGGGTAATGTAAATGGCTGG---
-------ATTAACATTTGGCAATAATTCATTCTACAATCCAAATATACTCTACAATGCAT
GGAACGTCTTTTCAATGGTGGATGATGAATATGTAATTTCCACGGAAACATTCGAATTTG
ACATATCAGAATTCGCAATGCAATCTTTAAGTAACTACTTCATGGACCAATACTTCCTAT
TGATTGAAGCATTCAATGCCTCTGATGTGCAAACATTGTCAACTATTTCAATCGAGTTGT
TGGATATCATCAANTACATGGATGAAATTGCAAGTACGCAAAGTTCTCTTCAATTGGGGT
TATGGACATACCGTGGTAGATTATGGGCCTACCCAACCAACGATATCCCAACCCTTCAAA
ATAGTAGTAACTCAAATACCGCACCATACGAATTCAATGCTCGTAATGTCTTAACTTTAT
GGGGTCCTTCAAATTCTGTACTCCATGATTACGCTTTCAAACTATGGAGTGGTTTAGTAA
GTGATTTNTATTCACCACGTTGGCAACTCTTTTTAAAGTCATTAGTGCAATCTGTTGAAA
ATCGTAAACCATTCAATAAGGAATCATTCAATAGA