>CHF451 (CHF451Q) /CSM/CH/CHF4-C/CHF451Q.Seq.d/
ATGCAATTCATGCTCAAAGTAAACGTGTTTTGGTAAACACTTGTAATATCACGCTTTCAT
CGATCCATCCAAATTATGGTCCAACTTCTGGTGGTTCATTGGTATCTATTAGTGGTTCTG
GTTTTAATTGTGGCATTAGTAATGTATTGTTTGGTGGTGCTGCATGCTCAAATCCAAAGG
TTAAAACAGATGGACTCTTAACTTGTACAACTCCTTCCTCTGATAATAATGGTCCAGCTG
ATATTGTTGTCAAAGGTTCTGATGGTAGTACCGTTACATTAACTGAAGCCTATAACTATG
TTTCAACCATTGCAATTACTTCATGTGCACTCGGTGCAAACAATGGTAATTTCTTTAGTT
GTGATATCTCTTCTCCAGTTGATACTTCAAATAGGCCTTCATGTAGTTCTCTTTTCTCTT
CTGCAACTTTACAAACTTTGGGTAGTTCTCCAAGATGTGAATATACTGCTTCAAACATTC
TAATGGTTACATTGGGTAGTGGTGAAAGTGTATCAGTTGGCCAACAATTGGAATTTTCTC
AAGGTGGAATCACTTCAAAAGATGGTAGTAAGAATAATTATCAAACTATAACATTGGAAC
CACXXXXXXXXXXTGGTATTTCAACAGTTGTCGATTTGATATCAACCTCTACAGATACTT
CAGCAGTTGTTCATCCATCAACTCTTAAAGAATCAATGAATATTATGAAAGTAGTTAGTC
CAATGATGGTTAAGTCACAAGTTTTACCAAGTGATTTCATTTTATCTGTTAACTATTTAA
GTAATGTCAATCAACAAGTCATTGGTCAACAATCAAATATCACTCAAGGTAAAACTGCAC
CAACCTATGCTGATATGGTTTATAATGTTTCTTCAGACATTTTAGTGGTATCCAATTCAT
TGGCAGATGGTATTCTTCAAAGAACTGCAATTGGTGAAGAACCATTTACAGTTGCAAGTG
ATAAAGGTTTCTCGATTACAACATTTAAAACTCTACATATGGAATTAAGTAAGAGTGGTC
AATCGATTGGTTTCAAAAAGGATGATGAAGGTCAATCTCTTGATGGTGAAAAACTTGCTT
TCACTCCAAAATTCAGTATTCCATCAAGTGTTTTATCAAAGAATAAATTAAAACAAACCT
CTGAAATCACTGGTAAACTAATTACAGTTCCATCAAATATTCATTTCAATCATAAATCTG
ATCAAACCATTTTCCCAAAAACTGTTTCACTTGAACTTTTCAATGGGGCTCTCAATTATC
AATCAAGGCCTTAAAGATCAAATTATCATNATAGGGAAAACATTCACC

AIHAQSKRVLVNTCNITLSSIHPNYGPTSGGSLVSISGSGFNCGISNVLFGGAACSNPKV
KTDGLLTCTTPSSDNNGPADIVVKGSDGSTVTLTEAYNYVSTIAITSCALGANNGNFFSC
DISSPVDTSNRPSCSSLFSSATLQTLGSSPRCEYTASNILMVTLGSGESVSVGQQLEFSQ
GGITSKDGSKNNYQTITLEP---

---GISTVVDLISTSTDTSAVVHPSTLKESMNIMKVVSPMMVKSQVLPSDFILSVNYLSN
VNQQVIGQQSNITQGKTAPTYADMVYNVSSDILVVSNSLADGILQRTAIGEEPFTVASDK
GFSITTFKTLHMELSKSGQSIGFKKDDEGQSLDGEKLAFTPKFSIPSSVLSKNKLKQTSE
ITGKLITVPSNIHFNHKSDQTIFPKTVSLELFNGALNYQSRP*rsnyhxrenih

Frame A:
mqfmlkvnvfw*tlvisrfhrsiqimvqllvvhwyllvvlvlivalvmyclvvlhaqiqr
lkqmds*lvqllpliimvqlillskvlmvvplh*lkpitmfqplqllhvhsvqtmvislv
visllqlilqiglhvvlfsllqlyklwvvlqdvnillqtf*wlhwvvvkvyqlannwnfl
kveslqkmvvriiikl*hwnh---

---wyfnscrfdinlyryfsscssins*rineyyess*sndg*vtsftk*fhfic*lfk*
cqstshwstikyhsr*nctnlc*ygl*cffrhfsgiqfigrwysskncnw*rtiysck**
rfldyni*nstygik*ewsidwfqkg**rsis*w*ktcfhskiqysikcfike*iktnl*
nhw*tnyssikysfqs*i*snhfpkncft*tfqwgsqlsikalkiklsx*gkhs

Frame B:
cnscsk*tcfgkhl*yhafidpsklwsnfwwfigiy*wfwf*lwh**civwwccmlkskg
*nrwtlnlynsfl***wss*yccqrf*w*yryin*sl*lcfnhcnyfmctrckqw*fl*l
*ylfss*yfk*afm*fsflfcnftnfg*fskm*iycfkhsngyig*w*kciswptigifs
rwnhfkrw**e*lsnynigt---

---GISTVVDLISTSTDTSAVVHPSTLKESMNIMKVVSPMMVKSQVLPSDFILSVNYLSN
VNQQVIGQQSNITQGKTAPTYADMVYNVSSDILVVSNSLADGILQRTAIGEEPFTVASDK
GFSITTFKTLHMELSKSGQSIGFKKDDEGQSLDGEKLAFTPKFSIPSSVLSKNKLKQTSE
ITGKLITVPSNIHFNHKSDQTIFPKTVSLELFNGALNYQSRP*rsnyhxrenih

Frame C:
AIHAQSKRVLVNTCNITLSSIHPNYGPTSGGSLVSISGSGFNCGISNVLFGGAACSNPKV
KTDGLLTCTTPSSDNNGPADIVVKGSDGSTVTLTEAYNYVSTIAITSCALGANNGNFFSC
DISSPVDTSNRPSCSSLFSSATLQTLGSSPRCEYTASNILMVTLGSGESVSVGQQLEFSQ
GGITSKDGSKNNYQTITLEP---

---vfqqlsi*yqplqilqqlfihqllknq*il*k*lvq*wlshkfyqvisfyllti*vm
sinkslvnnqislkvklhqpmliwfimflqtf*wypihwqmvffkelqlvknhlqlqvik
vsrlqhlklyiwn*vrvvnrlvskrmmkvnllmvknllslqnsvfhqvfyqrin*nkplk
slvn*lqfhqifisiinlikpfsqklfhlnfsmglsiinqglkdqiixigktft

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHF451 (CHF451Q) /CSM/CH/CHF4-C/CHF451Q.Seq.d/                       2527   0.0
SHD705 (SHD705Q) /CSM/SH/SHD7-A/SHD705Q.Seq.d/                       1176   0.0
CHF470 (CHF470Q) /CSM/CH/CHF4-C/CHF470Q.Seq.d/                       1170   0.0
SHD559 (SHD559Q) /CSM/SH/SHD5-C/SHD559Q.Seq.d/                       1100   0.0
SHG125 (SHG125Q) /CSM/SH/SHG1-B/SHG125Q.Seq.d/                       1076   0.0
CHR388 (CHR388Q) /CSM/CH/CHR3-D/CHR388Q.Seq.d/                       1076   0.0
CHP127 (CHP127Q) /CSM/CH/CHP1-B/CHP127Q.Seq.d/                       1072   0.0
CHG891 (CHG891Q) /CSM/CH/CHG8-D/CHG891Q.Seq.d/                       1049   0.0
CHQ695 (CHQ695Q) /CSM/CH/CHQ6-D/CHQ695Q.Seq.d/                       1033   0.0
SHI177 (SHI177Q) /CSM/SH/SHI1-D/SHI177Q.Seq.d/                       1023   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC103541|AC103541.6 Homo sapiens BAC clone RP11-818M13 from 4, complete sequence.    48   0.37   1
AC097518|AC097518.2 Homo sapiens BAC clone RP11-487E13 from 4, complete sequence.    48   0.37   1
AC024453|AC024453.2 Homo sapiens chromosome 4 clone RP11-2B17 map 4, WORKING DRAFT SEQUENCE, 51 unordered pieces.    48   0.37   1
AC013774|AC013774.3 Homo sapiens clone RP11-10P1, WORKING DRAFT SEQUENCE, 11 unordered pieces.    48   0.37   1
U66528|U66528.1 Dictyostelium discoideum CigB (cigB) mRNA, partial cds.    38   0.47   3
AJ410884|AJ410884.1 Influenza A virus (swine/Cotes d'Armor/1515/99 (H1N1)) partial N1NA  gene for Neuraminidase, genomic RNA.    42   0.68   2
AJ410883|AJ410883.1 Influenza A virus (swine/Cotes d'Armor/1482/99 (H1N1)) partial N1NA  gene for Neuraminidase, genomic RNA.    42   0.70   2
AC134522|AC134522.7 Medicago truncatula clone mth2-12g4, complete sequence.    40   0.96   8
AQ290526|AQ290526.1 nbxb0037A02r CUGI Rice BAC Library Oryza sativa genomic clone nbxb0037A02r, genomic survey sequence.    46   1.4    1
AC105973|AC105973.5 Mus musculus chromosome 6 clone RP24-257M4 map 6, *** SEQUENCING IN PROGRESS ***, 5 ordered pieces.    46   1.4    1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY269422_1(AY269422|pid:none) Candida albicans agglutinin-like s...    44   0.009
FM992693_338(FM992693|pid:none) Candida dubliniensis CD36 chromo...    43   0.027
CP000884_5785(CP000884|pid:none) Delftia acidovorans SPH-1, comp...    43   0.027
T34434(T34434) hypothetical protein K06A9.1a - Caenorhabditis el...    43   0.027
CP000478_2890(CP000478|pid:none) Syntrophobacter fumaroxidans MP...    42   0.046
(Q54JA5) RecName: Full=Integrin beta-like protein C; Flags: Prec...    40   0.17
AY269423_1(AY269423|pid:none) Candida albicans agglutinin-like s...    40   0.17
AY094967_1(AY094967|pid:none) Drosophila melanogaster SD07675 fu...    40   0.23
AL033534_13(AL033534|pid:none) S.pombe chromosome II cosmid c215...    40   0.23
CP000949_5030(CP000949|pid:none) Pseudomonas putida W619, comple...    40   0.23

 psg: 0.84  gvh: 0.50  alm: 0.41  top: 0.50  tms: 0.00  mit: 0.36  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: mitochondrial
	 36.0 %: nuclear
	 16.0 %: cytoplasmic
	  8.0 %: cytoskeletal
	  4.0 %: endoplasmic reticulum

>> prediction for CHF451 is mit

>CHF451F.Seq
ATGCAATTCATGCTCAAAGTAAACGTGTTTTGGTAAACACTTGTAATATCACGCTTTCAT
CGATCCATCCAAATTATGGTCCAACTTCTGGTGGTTCATTGGTATCTATTAGTGGTTCTG
GTTTTAATTGTGGCATTAGTAATGTATTGTTTGGTGGTGCTGCATGCTCAAATCCAAAGG
TTAAAACAGATGGACTCTTAACTTGTACAACTCCTTCCTCTGATAATAATGGTCCAGCTG
ATATTGTTGTCAAAGGTTCTGATGGTAGTACCGTTACATTAACTGAAGCCTATAACTATG
TTTCAACCATTGCAATTACTTCATGTGCACTCGGTGCAAACAATGGTAATTTCTTTAGTT
GTGATATCTCTTCTCCAGTTGATACTTCAAATAGGCCTTCATGTAGTTCTCTTTTCTCTT
CTGCAACTTTACAAACTTTGGGTAGTTCTCCAAGATGTGAATATACTGCTTCAAACATTC
TAATGGTTACATTGGGTAGTGGTGAAAGTGTATCAGTTGGCCAACAATTGGAATTTTCTC
AAGGTGGAATCACTTCAAAAGATGGTAGTAAGAATAATTATCAAACTATAACATTGGAAC
CACNNNNNNNNNN

>CHF451Z.Seq
NNNNNNNNNNTGGTATTTCAACAGTTGTCGATTTGATATCAACCTCTACAGATACTTCAG
CAGTTGTTCATCCATCAACTCTTAAAGAATCAATGAATATTATGAAAGTAGTTAGTCCAA
TGATGGTTAAGTCACAAGTTTTACCAAGTGATTTCATTTTATCTGTTAACTATTTAAGTA
ATGTCAATCAACAAGTCATTGGTCAACAATCAAATATCACTCAAGGTAAAACTGCACCAA
CCTATGCTGATATGGTTTATAATGTTTCTTCAGACATTTTAGTGGTATCCAATTCATTGG
CAGATGGTATTCTTCAAAGAACTGCAATTGGTGAAGAACCATTTACAGTTGCAAGTGATA
AAGGTTTCTCGATTACAACATTTAAAACTCTACATATGGAATTAAGTAAGAGTGGTCAAT
CGATTGGTTTCAAAAAGGATGATGAAGGTCAATCTCTTGATGGTGAAAAACTTGCTTTCA
CTCCAAAATTCAGTATTCCATCAAGTGTTTTATCAAAGAATAAATTAAAACAAACCTCTG
AAATCACTGGTAAACTAATTACAGTTCCATCAAATATTCATTTCAATCATAAATCTGATC
AAACCATTTTCCCAAAAACTGTTTCACTTGAACTTTTCAATGGGGCTCTCAATTATCAAT
CAAGGCCTTAAAGATCAAATTATCATNATAGGGAAAACATTCACC

>CHF451P.Seq
ATGCAATTCATGCTCAAAGTAAACGTGTTTTGGTAAACACTTGTAATATCACGCTTTCAT
CGATCCATCCAAATTATGGTCCAACTTCTGGTGGTTCATTGGTATCTATTAGTGGTTCTG
GTTTTAATTGTGGCATTAGTAATGTATTGTTTGGTGGTGCTGCATGCTCAAATCCAAAGG
TTAAAACAGATGGACTCTTAACTTGTACAACTCCTTCCTCTGATAATAATGGTCCAGCTG
ATATTGTTGTCAAAGGTTCTGATGGTAGTACCGTTACATTAACTGAAGCCTATAACTATG
TTTCAACCATTGCAATTACTTCATGTGCACTCGGTGCAAACAATGGTAATTTCTTTAGTT
GTGATATCTCTTCTCCAGTTGATACTTCAAATAGGCCTTCATGTAGTTCTCTTTTCTCTT
CTGCAACTTTACAAACTTTGGGTAGTTCTCCAAGATGTGAATATACTGCTTCAAACATTC
TAATGGTTACATTGGGTAGTGGTGAAAGTGTATCAGTTGGCCAACAATTGGAATTTTCTC
AAGGTGGAATCACTTCAAAAGATGGTAGTAAGAATAATTATCAAACTATAACATTGGAAC
CAC----------TGGTATTTCAACAGTTGTCGATTTGATATCAACCTCTACAGATACTT
CAGCAGTTGTTCATCCATCAACTCTTAAAGAATCAATGAATATTATGAAAGTAGTTAGTC
CAATGATGGTTAAGTCACAAGTTTTACCAAGTGATTTCATTTTATCTGTTAACTATTTAA
GTAATGTCAATCAACAAGTCATTGGTCAACAATCAAATATCACTCAAGGTAAAACTGCAC
CAACCTATGCTGATATGGTTTATAATGTTTCTTCAGACATTTTAGTGGTATCCAATTCAT
TGGCAGATGGTATTCTTCAAAGAACTGCAATTGGTGAAGAACCATTTACAGTTGCAAGTG
ATAAAGGTTTCTCGATTACAACATTTAAAACTCTACATATGGAATTAAGTAAGAGTGGTC
AATCGATTGGTTTCAAAAAGGATGATGAAGGTCAATCTCTTGATGGTGAAAAACTTGCTT
TCACTCCAAAATTCAGTATTCCATCAAGTGTTTTATCAAAGAATAAATTAAAACAAACCT
CTGAAATCACTGGTAAACTAATTACAGTTCCATCAAATATTCATTTCAATCATAAATCTG
ATCAAACCATTTTCCCAAAAACTGTTTCACTTGAACTTTTCAATGGGGCTCTCAATTATC
AATCAAGGCCTTAAAGATCAAATTATCATNATAGGGAAAACATTCACC