NBDC - アーカイブトップ - ヘルプ
データベースの説明 | データ項目の説明 | 利用許諾 | ヘルプ
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
Description of this DB | Description of data items | Download | License
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CHF424
Library CH
(Link to library)
Clone ID CHF424
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID CHF424P
(Link to Original site)
Representative DNA sequence 
>CHF424 (CHF424Q) /CSM/CH/CHF4-A/CHF424Q.Seq.d/
AAGTTAATTCAGTAGTTCAATTTAATTTATAATAATAATAATAGTATAAATATATAAAGA
TTAATTTTTTTGTATATAAATTTTTGTAAATAAATAAATAAATAATAATTTTTTTTTTAA
TTCACACACATACATATAAATATTAAATAATTAAATATTAAAAAATGTCATCAATTGGTA
TTGATTTAGGTACAACATATTCATGTGTTGGTGTTTGGCAAAATGATCGTGTTGAAATTA
TTGCAAATGGTCAAGGTAATANAACCACTCCATCTTATGTTGCATTTACTGATACAGAAC
GTTTAATTGGTGATGCTGCAAAGAATCAAGTTGCAATGAATCCAACCAATACAGTATTTG
ATGCTAAACGTTTAATTGGTCGTAAATTTAGTGACAAAGAAGTTCAAAGTGATATGAAAC
ATTGGCCATTCAAAGTTATTCCAAAGGATGGTGATAAGCCACATATTCAAGTTGAATTCA
AAGGTGAAACTAAAGTTTTCTCACCANAAGAAATCTCTTCAATGGTACTCTTAAAAATGA
AAGAAACCGCTGAAGCTTATCTTGGTAAAACCATTAATAATGCTGTAATTACCGXXXXXX
XXXXTTATTCCAAGAAATACAACCATTTCCATGTAAGAAAACTCCAAACCTTCTCTACAT
ACTCTGATAATCAACCAGGTGTATTAATTCAAGTTTATGAAGGTGAACGTGCTATGACCA
AAGATAATAATTTATTGGGTAAATTTGAACTTTCTGGTATTCCACCAGCACCAAGAGGTG
TACCACAAGTTGAAGTTACATTTGATGTTGATGCAAATGGTATCTTAAATGTATCAGCTG
AAGATAAATCAACTGGTAACAAACAAAAGATTACAATTACCAATGATAAAGGTCGTCTTT
CAAAAGAAGAAATTGAAAAAATGGTTGCTGATGCAGAGAAATTCAAACAACAAGATGAAC
AACAAAAAGATCGTGTTGAATCAAAGAATAAATTGGAAAATTATGCATTCACTGTTAAAA
ATTCAATTAAAGATGAAAAAGTTGCAGCCAAAATTTCAGATTCAGATAAATCAACTATTG
AATCTGAAACTGAAAGTGTACTCAAATGGTTAGAATCAAATCAAACCGCAGAAAAGGATG
AATATGAAGATAAGATGAAAGCCTTAGAAGCAGTTGTCAATCCAATCATGAGTAAACTCT
ATCAAGAAGGAGGTATGCCACAAGGTGGTGGTATGCCAGGTGGTATGTCAAATGATTCAC
CAAAGAGTTCAAACAACAAAGTAGACGAATTAGATTGACGGAATTTTTCAGAGGAAAACA
TCTCTATCAAATAGATTTCTTAAAAAATAATAAGTTAGTAAAATCAATAAAGCTTNCTCA
TATATAGTACACA
sequence update 2002.10.25
Translated Amino Acid sequence 
vnsvvqfnl*****ykyikinffvykfl*ink*iiifflihthtyky*iiky*KMSSIGI
DLGTTYSCVGVWQNDRVEIIANGQGNXTTPSYVAFTDTERLIGDAAKNQVAMNPTNTVFD
AKRLIGRKFSDKEVQSDMKHWPFKVIPKDGDKPHIQVEFKGETKVFSPXEISSMVLLKMK
ETAEAYLGKTINNAVIT---

---YSKKYNHFHVRKLQTFSTYSDNQPGVLIQVYEGERAMTKDNNLLGKFELSGIPPAPR
GVPQVEVTFDVDANGILNVSAEDKSTGNKQKITITNDKGRLSKEEIEKMVADAEKFKQQD
EQQKDRVESKNKLENYAFTVKNSIKDEKVAAKISDSDKSTIESETESVLKWLESNQTAEK
DEYEDKMKALEAVVNPIMSKLYQEGGMPQGGGMPGGMSNDSPKSSNNKVDELD*rnfsee
nisik*is*kiis**nq*sxliyst
Translated Amino Acid sequence (All Frames) 
Frame A:
kliq*fnliynnnnsini*rliflyinfck*ink**fff*fthihinik*lnikkchqlv
li*vqhihvlvfgkmivlkllqmvkvixplhlmlhlliqnv*lvmlqriklq*iqpiqyl
mlnv*lvvnlvtkkfkvi*nighsklfqrmvishifklnskvklkfshxkkslqwys*k*
kkplklilvkpliml*lp---

---lfqeiqpfpckktpnllyil**strcinssl*r*tcydqr**fig*i*tfwyststk
rctts*syi*c*ckwylkcis*r*inw*qtkdynyq**rssfkrrn*kngc*creiqttr
*ttkrsc*ike*igklcihc*kfn*r*kscsqnfrfr*iny*i*n*kctqmvriksnrrk
g*i*r*deslrsscqsnhe*tlsrrryatrwwyarwyvk*ftkefkqqsrrirlteffrg
khlyqidflknnklvksiklxhi*yt

Frame B:
s*fsssi*fiiiiiv*iykd*ffci*ifvnk*innnfffnshtyi*ilnn*ilknvinwy
*frynifmcwclak*sc*nyckwsr*xnhsilcciy*yrtfnw*cckesscnesnqysi*
c*tfnws*i**qrssk*yetlaiqsyskgw**atyss*iqr*n*sfltxrnlfngtlkne
rnr*slsw*nh**ccny---

---YSKKYNHFHVRKLQTFSTYSDNQPGVLIQVYEGERAMTKDNNLLGKFELSGIPPAPR
GVPQVEVTFDVDANGILNVSAEDKSTGNKQKITITNDKGRLSKEEIEKMVADAEKFKQQD
EQQKDRVESKNKLENYAFTVKNSIKDEKVAAKISDSDKSTIESETESVLKWLESNQTAEK
DEYEDKMKALEAVVNPIMSKLYQEGGMPQGGGMPGGMSNDSPKSSNNKVDELD*rnfsee
nisik*is*kiis**nq*sxliyst

Frame C:
vnsvvqfnl*****ykyikinffvykfl*ink*iiifflihthtyky*iiky*KMSSIGI
DLGTTYSCVGVWQNDRVEIIANGQGNXTTPSYVAFTDTERLIGDAAKNQVAMNPTNTVFD
AKRLIGRKFSDKEVQSDMKHWPFKVIPKDGDKPHIQVEFKGETKVFSPXEISSMVLLKMK
ETAEAYLGKTINNAVIT---

---iprnttism*enskpslhtliinqvy*fkfmkvnvl*pkiiiywvnlnflvfhqhqe
vyhklklhlmlmqmvs*myqlkinqlvtnkrlqlpmikvvfqkkklkkwllmqrnsnnkm
nnkkivlnqrinwkimhsllkiqlkmkklqpkfqiqinqllnlklkvysng*nqikpqkr
mnmkir*kp*kqlsiqs*vnsikkevchkvvvcqvvcqmihqrvqttk*tn*idgifqrk
tslsnrflkk**vskinkaxsyivh
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHF424 (CHF424Q) /CSM/CH/CHF4-A/CHF424Q.Seq.d/                       2450   0.0
VHK415 (VHK415Q) /CSM/VH/VHK4-A/VHK415Q.Seq.d/                       1534   0.0
CHI672 (CHI672Q) /CSM/CH/CHI6-C/CHI672Q.Seq.d/                       1534   0.0
AHM473 (AHM473Q) /CSM/AH/AHM4-D/AHM473Q.Seq.d/                       1532   0.0
CHQ771 (CHQ771Q) /CSM/CH/CHQ7-C/CHQ771Q.Seq.d/                       1530   0.0
CHI127 (CHI127Q) /CSM/CH/CHI1-B/CHI127Q.Seq.d/                       1526   0.0
AHA169 (AHA169Q) /CSM/AH/AHA1-C/AHA169Q.Seq.d/                       1524   0.0
CHS374 (CHS374Q) /CSM/CH/CHS3-D/CHS374Q.Seq.d/                       1522   0.0
CHF190 (CHF190Q) /CSM/CH/CHF1-D/CHF190Q.Seq.d/                       1518   0.0
AHD349 (AHD349Q) /CSM/AH/AHD3-C/AHD349Q.Seq.d/                       1518   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

X75263|X75263.1 D.discoideum (AX3) mRNA for heat shock cognate protein (hsc70).  1449   0.0    4
L22736|L22736.1 Dictyostelium discoideum heat shock protein (hspB) gene,.  1441   0.0    4
AF025951|AF025951.1 Dictyostelium discoideum heat-shock cognate protein 70 (hsc70) mRNA, complete cds.   238   e-138  7
AC115684|AC115684.2 Dictyostelium discoideum chromosome 2 map 3108975-3191114 strain AX4, complete sequence.   238   e-130  13
AF221541|AF221541.1 Cryptosporidium serpentis heat shock protein 70 (HSP70) gene, partial cds.   131   1e-96  10
A58313|A58313.1 Sequence 1 from Patent WO9636707.                 133   2e-90  7
Z30210|Z30210.1 C.albicans (ATCC 20955) cahsp70 mRNA for heat shock protein 70.   133   6e-90  7
AX536934|AX536934.1 Sequence 535 from Patent WO02064766.          133   5e-88  7
M84652|M84652.1 Entamoeba heat shock protein (Hsp70) mRNA, complete cds.   159   4e-85  7
X90948|X90948.1 A.nodosum mRNA for HSP70 protein.                 107   7e-78  8
dna update 2004. 9.28
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P36415) RecName: Full=Heat shock cognate 70 kDa protein 1;     ...   368   e-100
S37394(S37394) dnaK-type molecular chaperone hsc70 - slime mold ...   368   e-100
AK303980_1(AK303980|pid:none) Homo sapiens cDNA FLJ54342 complet...   367   e-100
AK097824_1(AK097824|pid:none) Homo sapiens cDNA FLJ40505 fis, cl...   362   2e-98
AF033852_1(AF033852|pid:none) Spinacia oleracea cytosolic heat s...   271   5e-71
FB790108_1(FB790108|pid:none) Sequence 9381 from Patent WO200803...   270   7e-71
EF506932_1(EF506932|pid:none) Fasciola hepatica heat shock prote...   270   7e-71
EF506931_1(EF506931|pid:none) Fasciola gigantica heat shock prot...   269   1e-70
FB790112_1(FB790112|pid:none) Sequence 9385 from Patent WO200803...   269   1e-70
(P22953) RecName: Full=Heat shock cognate 70 kDa protein 1;     ...   269   2e-70
protein update 2009. 3.31
PSORT 

 psg: 0.87  gvh: 0.35  alm: 0.46  top: 0.27  tms: 0.00  mit: 0.19  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 32.0 %: nuclear
	  8.0 %: vacuolar
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for CHF424 is cyt
5' end seq. ID CHF424F
5' end seq. 
>CHF424F.Seq
AAGTTAATTCAGTAGTTCAATTTAATTTATAATAATAATAATAGTATAAATATATAAAGA
TTAATTTTTTTGTATATAAATTTTTGTAAATAAATAAATAAATAATAATTTTTTTTTTAA
TTCACACACATACATATAAATATTAAATAATTAAATATTAAAAAATGTCATCAATTGGTA
TTGATTTAGGTACAACATATTCATGTGTTGGTGTTTGGCAAAATGATCGTGTTGAAATTA
TTGCAAATGGTCAAGGTAATANAACCACTCCATCTTATGTTGCATTTACTGATACAGAAC
GTTTAATTGGTGATGCTGCAAAGAATCAAGTTGCAATGAATCCAACCAATACAGTATTTG
ATGCTAAACGTTTAATTGGTCGTAAATTTAGTGACAAAGAAGTTCAAAGTGATATGAAAC
ATTGGCCATTCAAAGTTATTCCAAAGGATGGTGATAAGCCACATATTCAAGTTGAATTCA
AAGGTGAAACTAAAGTTTTCTCACCANAAGAAATCTCTTCAATGGTACTCTTAAAAATGA
AAGAAACCGCTGAAGCTTATCTTGGTAAAACCATTAATAATGCTGTAATTACCGNNNNNN
NNNN
Length of 5' end seq. 604
3' end seq. ID CHF424Z
3' end seq. 
>CHF424Z.Seq
NNNNNNNNNNTTATTCCAAGAAATACAACCATTTCCATGTAAGAAAACTCCAAACCTTCT
CTACATACTCTGATAATCAACCAGGTGTATTAATTCAAGTTTATGAAGGTGAACGTGCTA
TGACCAAAGATAATAATTTATTGGGTAAATTTGAACTTTCTGGTATTCCACCAGCACCAA
GAGGTGTACCACAAGTTGAAGTTACATTTGATGTTGATGCAAATGGTATCTTAAATGTAT
CAGCTGAAGATAAATCAACTGGTAACAAACAAAAGATTACAATTACCAATGATAAAGGTC
GTCTTTCAAAAGAAGAAATTGAAAAAATGGTTGCTGATGCAGAGAAATTCAAACAACAAG
ATGAACAACAAAAAGATCGTGTTGAATCAAAGAATAAATTGGAAAATTATGCATTCACTG
TTAAAAATTCAATTAAAGATGAAAAAGTTGCAGCCAAAATTTCAGATTCAGATAAATCAA
CTATTGAATCTGAAACTGAAAGTGTACTCAAATGGTTAGAATCAAATCAAACCGCAGAAA
AGGATGAATATGAAGATAAGATGAAAGCCTTAGAAGCAGTTGTCAATCCAATCATGAGTA
AACTCTATCAAGAAGGAGGTATGCCACAAGGTGGTGGTATGCCAGGTGGTATGTCAAATG
ATTCACCAAAGAGTTCAAACAACAAAGTAGACGAATTAGATTGACGGAATTTTTCAGAGG
AAAACATCTCTATCAAATAGATTTCTTAAAAAATAATAAGTTAGTAAAATCAATAAAGCT
TNCTCATATATAGTACACA
Length of 3' end seq. 799
Connected seq. ID CHF424P
Connected seq. 
>CHF424P.Seq
AAGTTAATTCAGTAGTTCAATTTAATTTATAATAATAATAATAGTATAAATATATAAAGA
TTAATTTTTTTGTATATAAATTTTTGTAAATAAATAAATAAATAATAATTTTTTTTTTAA
TTCACACACATACATATAAATATTAAATAATTAAATATTAAAAAATGTCATCAATTGGTA
TTGATTTAGGTACAACATATTCATGTGTTGGTGTTTGGCAAAATGATCGTGTTGAAATTA
TTGCAAATGGTCAAGGTAATANAACCACTCCATCTTATGTTGCATTTACTGATACAGAAC
GTTTAATTGGTGATGCTGCAAAGAATCAAGTTGCAATGAATCCAACCAATACAGTATTTG
ATGCTAAACGTTTAATTGGTCGTAAATTTAGTGACAAAGAAGTTCAAAGTGATATGAAAC
ATTGGCCATTCAAAGTTATTCCAAAGGATGGTGATAAGCCACATATTCAAGTTGAATTCA
AAGGTGAAACTAAAGTTTTCTCACCANAAGAAATCTCTTCAATGGTACTCTTAAAAATGA
AAGAAACCGCTGAAGCTTATCTTGGTAAAACCATTAATAATGCTGTAATTACCG------
----TTATTCCAAGAAATACAACCATTTCCATGTAAGAAAACTCCAAACCTTCTCTACAT
ACTCTGATAATCAACCAGGTGTATTAATTCAAGTTTATGAAGGTGAACGTGCTATGACCA
AAGATAATAATTTATTGGGTAAATTTGAACTTTCTGGTATTCCACCAGCACCAAGAGGTG
TACCACAAGTTGAAGTTACATTTGATGTTGATGCAAATGGTATCTTAAATGTATCAGCTG
AAGATAAATCAACTGGTAACAAACAAAAGATTACAATTACCAATGATAAAGGTCGTCTTT
CAAAAGAAGAAATTGAAAAAATGGTTGCTGATGCAGAGAAATTCAAACAACAAGATGAAC
AACAAAAAGATCGTGTTGAATCAAAGAATAAATTGGAAAATTATGCATTCACTGTTAAAA
ATTCAATTAAAGATGAAAAAGTTGCAGCCAAAATTTCAGATTCAGATAAATCAACTATTG
AATCTGAAACTGAAAGTGTACTCAAATGGTTAGAATCAAATCAAACCGCAGAAAAGGATG
AATATGAAGATAAGATGAAAGCCTTAGAAGCAGTTGTCAATCCAATCATGAGTAAACTCT
ATCAAGAAGGAGGTATGCCACAAGGTGGTGGTATGCCAGGTGGTATGTCAAATGATTCAC
CAAAGAGTTCAAACAACAAAGTAGACGAATTAGATTGACGGAATTTTTCAGAGGAAAACA
TCTCTATCAAATAGATTTCTTAAAAAATAATAAGTTAGTAAAATCAATAAAGCTTNCTCA
TATATAGTACACA
Length of connected seq. 1383
Full length Seq ID -
Full length Seq. -
Length of full length seq. -