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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CHF394
Library CH
(Link to library)
Clone ID CHF394
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15817-1
Original site URL
Representative seq. ID CHF394P
(Link to Original site)
Representative DNA sequence 
>CHF394 (CHF394Q) /CSM/CH/CHF3-D/CHF394Q.Seq.d/
AAATAGTTTAATAAATAAAAATAAACAAAACAAATAAAAAATAAATAAATAAAAAAAATG
AAAACAGTTACATTAGCAACATGTAACTTAAATCAATGGGCAATGGATTTCAAAGGAAAT
CTTGAAAGAATAATAGAATCGATTAATATTGCAAAATCTAAAGGAGCAAAATATAGATTA
GGACCAGAATTAGAAATTTGTGGATATGGTTGTGAAGATCATTTTTTAGAACAAGATACA
ATGTTACATTGTTGGCAAAGTTTAGCAGTAATATTAAAGGATCCAGAGTTAACAAAGGAT
ATTTTAGTTGATGTTGGTATGCCAGTATTACATAAGGATGTTAGATATAATTGTAGAGTC
ATATTATTGAATCAAAAGATTTATTTAATACAACCAAAGAAAGCCATGGCAATGGATGGT
AATTATAGGGAAGGTCGTTGGTTTACACCATGGATTAAACCAAGAGTTGTGGAAACTTTT
TATCTTCCAAGGATCATTAGTCAAATCACTGGTCAGGATGAATGCCAAATCGGTGATGCA
ATCATTTCAACACTTGACACAGCCATTTCGTCAGAGACATGTGAGGAGTTATTTACACCA
AATTCACCACATATTCAAATGGGTTTAGATGGTGTTGAAATCTTTXXXXXXXXXXTAAAT
TCAGANATATGAAATCAATTCTCTCTGTACTCACAGCAACACCAACCNCTGAATTAGAAC
CAATCCNTGANAACTATACACAATCCGATGAAATTGATATGGGTATGNCCTATNAAGAAT
TATCAANNTTTGGTAAATTAAGAAAAGTCAATCGTTGTGGTCCAGTTTCAATGTTTGAAC
GTTTGGTTGCAGATTGGGCACATTTAGAACCATCAGTTGNGGCTNAAAAAGTTAAACGTT
TCTTTTATTANTANGCAATCAATCGTCATAAATTAACAACTTTAACACCATCCTATCATG
CTGAAGGTTATTCTCCTGATGATAATCGTTATGATCATCGTCAATTCCTTTACAATTCAA
AATGGGATGTTC
sequence update 2002.10.25
Translated Amino Acid sequence 
k*fnk*k*tkqiknk*IKKMKTVTLATCNLNQWAMDFKGNLERIIESINIAKSKGAKYRL
GPELEICGYGCEDHFLEQDTMLHCWQSLAVILKDPELTKDILVDVGMPVLHKDVRYNCRV
ILLNQKIYLIQPKKAMAMDGNYREGRWFTPWIKPRVVETFYLPRIISQITGQDECQIGDA
IISTLDTAISSETCEELFTPNSPHIQMGLDGVEIF---

---KFRXMKSILSVLTATPTXELEPIXXNYTQSDEIDMGMXYXELSXFGKLRKVNRCGPV
SMFERLVADWAHLEPSVXAXKVKRFFYXXAINRHKLTTLTPSYHAEGYSPDDNRYDHRQF
LYNSKWDV
Translated Amino Acid sequence (All Frames) 
Frame A:
k*fnk*k*tkqiknk*IKKMKTVTLATCNLNQWAMDFKGNLERIIESINIAKSKGAKYRL
GPELEICGYGCEDHFLEQDTMLHCWQSLAVILKDPELTKDILVDVGMPVLHKDVRYNCRV
ILLNQKIYLIQPKKAMAMDGNYREGRWFTPWIKPRVVETFYLPRIISQITGQDECQIGDA
IISTLDTAISSETCEELFTPNSPHIQMGLDGVEIF---

---*iqxyeinslcthsntnx*irtnp*xlytir*n*ygyxlxriixxw*ikksqslwss
fnv*tfgcrlgtfrtisxgxks*tflllxxnqss*innfntilsc*rlfs***sl*sssi
plqfkmgc

Frame B:
nslinknkqnk*kink*kk*kqlh*qhvt*ingqwiskeilke**nrlilqnlkeqnid*
dqn*kfvdmvvkiif*nkiqcyivgkv*q*y*riqs*qrif*lmlvcqyyirmldiives
yy*ikrfi*ynqrkpwqwmviigkvvglhhglnqelwklfifqgslvkslvrmnaksvmq
sfqhltqpfrqrhvrsylhqihhifkwv*mvlks---

---KFRXMKSILSVLTATPTXELEPIXXNYTQSDEIDMGMXYXELSXFGKLRKVNRCGPV
SMFERLVADWAHLEPSVXAXKVKRFFYXXAINRHKLTTLTPSYHAEGYSPDDNRYDHRQF
LYNSKWDV

Frame C:
iv**ikinktnkk*inkknensyisnm*lksmgngfqrks*knnrid*ycki*rski*ir
trirnlwiwl*rsffrtrynvtllakfssnikgsrvnkgyfs*cwyasit*gc*i*l*sh
iieskdlfnttkeshgngw*l*grslvytmd*tkscgnflsskdh*snhwsg*mpnr*cn
hfnt*hshfvrdm*gviytkfttysngfrwc*nl---

---nsxi*nqfslysqqhqpln*nqsxxtihnpmkliwvxpxknyqxlvn*eksivvvqf
qclnvwlqighi*nhqlxlkklnvsfixxqsivin*ql*hhpimlkvillmiivmiivns
ftiqngmf
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHF394 (CHF394Q) /CSM/CH/CHF3-D/CHF394Q.Seq.d/                       1788   0.0
CHN685 (CHN685Q) /CSM/CH/CHN6-D/CHN685Q.Seq.d/                       1152   0.0
CHJ352 (CHJ352Q) /CSM/CH/CHJ3-C/CHJ352Q.Seq.d/                       1152   0.0
CHG530 (CHG530Q) /CSM/CH/CHG5-B/CHG530Q.Seq.d/                       1152   0.0
CHO317 (CHO317Q) /CSM/CH/CHO3-A/CHO317Q.Seq.d/                       1138   0.0
CHF157 (CHF157Q) /CSM/CH/CHF1-C/CHF157Q.Seq.d/                       1124   0.0
CHR319 (CHR319Q) /CSM/CH/CHR3-A/CHR319Q.Seq.d/                       1116   0.0
CHO186 (CHO186Q) /CSM/CH/CHO1-D/CHO186Q.Seq.d/                       1114   0.0
CHF372 (CHF372Q) /CSM/CH/CHF3-C/CHF372Q.Seq.d/                       1114   0.0
CHI370 (CHI370Q) /CSM/CH/CHI3-C/CHI370Q.Seq.d/                       1110   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

BP128471|BP128471.1 Nicotiana tabacum cDNA, clone: BY223, primer: M13 forward, sequence 1.    56   4e-07  2
AB126258|AB126258.1 Nicotiana tabacum mRNA for NAD synthetase, partial cds.    56   5e-07  2
CR382133|CR382133.1 Debaryomyces hansenii chromosome A of strain CBS767 of Debaryomyces hansenii.    52   7e-07  17
BQ587794|BQ587794.1 S014308-024-009-K22-SP6 MPIZ-ADIS-024-leaf Beta vulgaris cDNA clone 024-009-K22 5-PRIME, mRNA sequence.    50   1e-06  2
BF053584|BF053584.1 EST438814 potato leaves and petioles Solanum tuberosum cDNA clone cSTB36M20 5' sequence, mRNA sequence.    64   5e-06  1
AL023704|AL023704.2 S.pombe chromosome III cosmid c553.            38   6e-05  5
CB671440|CB671440.1 OSJNEe05A13.r OSJNEe Oryza sativa (japonica cultivar-group) cDNA clone OSJNEe05A13 3', mRNA sequence.    40   0.063  2
CB635972|CB635972.1 ---.    40   0.069  2
CB628177|CB628177.1 ---.    40   0.070  2
AK063292|AK063292.1 Oryza sativa (japonica cultivar-group) cDNA clone:001-113-E04, full insert sequence.    40   0.16   2
dna update 2004. 9.28
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q5ZMA6) RecName: Full=Glutamine-dependent NAD(+) synthetase;   ...   272   1e-71
(Q3ZBF0) RecName: Full=Glutamine-dependent NAD(+) synthetase;   ...   272   1e-71
BC161121_1(BC161121|pid:none) Xenopus tropicalis hypothetical pr...   270   4e-71
AK001493_1(AK001493|pid:none) Homo sapiens cDNA FLJ10631 fis, cl...   270   7e-71
(Q6IA69) RecName: Full=Glutamine-dependent NAD(+) synthetase;   ...   268   2e-70
AB091316_1(AB091316|pid:none) Homo sapiens mRNA for glutamine-de...   268   2e-70
BC038016_1(BC038016|pid:none) Mus musculus NAD synthetase 1, mRN...   261   2e-68
AE016820_727(AE016820|pid:none) Ashbya gossypii (= Eremothecium ...   260   6e-68
CR380956_461(CR380956|pid:none) Candida glabrata strain CBS138 c...   259   8e-68
BC161832_1(BC161832|pid:none) Rattus norvegicus NAD synthetase 1...   259   1e-67
protein update 2009. 3.31
PSORT 

 psg: 0.85  gvh: 0.30  alm: 0.47  top: 0.20  tms: 0.00  mit: 0.30  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.50  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: nuclear
	 24.0 %: cytoplasmic
	 20.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: endoplasmic reticulum

>> prediction for CHF394 is nuc
5' end seq. ID CHF394F
5' end seq. 
>CHF394F.Seq
AAATAGTTTAATAAATAAAAATAAACAAAACAAATAAAAAATAAATAAATAAAAAAAATG
AAAACAGTTACATTAGCAACATGTAACTTAAATCAATGGGCAATGGATTTCAAAGGAAAT
CTTGAAAGAATAATAGAATCGATTAATATTGCAAAATCTAAAGGAGCAAAATATAGATTA
GGACCAGAATTAGAAATTTGTGGATATGGTTGTGAAGATCATTTTTTAGAACAAGATACA
ATGTTACATTGTTGGCAAAGTTTAGCAGTAATATTAAAGGATCCAGAGTTAACAAAGGAT
ATTTTAGTTGATGTTGGTATGCCAGTATTACATAAGGATGTTAGATATAATTGTAGAGTC
ATATTATTGAATCAAAAGATTTATTTAATACAACCAAAGAAAGCCATGGCAATGGATGGT
AATTATAGGGAAGGTCGTTGGTTTACACCATGGATTAAACCAAGAGTTGTGGAAACTTTT
TATCTTCCAAGGATCATTAGTCAAATCACTGGTCAGGATGAATGCCAAATCGGTGATGCA
ATCATTTCAACACTTGACACAGCCATTTCGTCAGAGACATGTGAGGAGTTATTTACACCA
AATTCACCACATATTCAAATGGGTTTAGATGGTGTTGAAATCTTTNNNNNNNNNN
Length of 5' end seq. 655
3' end seq. ID CHF394Z
3' end seq. 
>CHF394Z.Seq
NNNNNNNNNNTAAATTCAGANATATGAAATCAATTCTCTCTGTACTCACAGCAACACCAA
CCNCTGAATTAGAACCAATCCNTGANAACTATACACAATCCGATGAAATTGATATGGGTA
TGNCCTATNAAGAATTATCAANNTTTGGTAAATTAAGAAAAGTCAATCGTTGTGGTCCAG
TTTCAATGTTTGAACGTTTGGTTGCAGATTGGGCACATTTAGAACCATCAGTTGNGGCTN
AAAAAGTTAAACGTTTCTTTTATTANTANGCAATCAATCGTCATAAATTAACAACTTTAA
CACCATCCTATCATGCTGAAGGTTATTCTCCTGATGATAATCGTTATGATCATCGTCAAT
TCCTTTACAATTCAAAATGGGATGTTC
Length of 3' end seq. 387
Connected seq. ID CHF394P
Connected seq. 
>CHF394P.Seq
AAATAGTTTAATAAATAAAAATAAACAAAACAAATAAAAAATAAATAAATAAAAAAAATG
AAAACAGTTACATTAGCAACATGTAACTTAAATCAATGGGCAATGGATTTCAAAGGAAAT
CTTGAAAGAATAATAGAATCGATTAATATTGCAAAATCTAAAGGAGCAAAATATAGATTA
GGACCAGAATTAGAAATTTGTGGATATGGTTGTGAAGATCATTTTTTAGAACAAGATACA
ATGTTACATTGTTGGCAAAGTTTAGCAGTAATATTAAAGGATCCAGAGTTAACAAAGGAT
ATTTTAGTTGATGTTGGTATGCCAGTATTACATAAGGATGTTAGATATAATTGTAGAGTC
ATATTATTGAATCAAAAGATTTATTTAATACAACCAAAGAAAGCCATGGCAATGGATGGT
AATTATAGGGAAGGTCGTTGGTTTACACCATGGATTAAACCAAGAGTTGTGGAAACTTTT
TATCTTCCAAGGATCATTAGTCAAATCACTGGTCAGGATGAATGCCAAATCGGTGATGCA
ATCATTTCAACACTTGACACAGCCATTTCGTCAGAGACATGTGAGGAGTTATTTACACCA
AATTCACCACATATTCAAATGGGTTTAGATGGTGTTGAAATCTTT----------TAAAT
TCAGANATATGAAATCAATTCTCTCTGTACTCACAGCAACACCAACCNCTGAATTAGAAC
CAATCCNTGANAACTATACACAATCCGATGAAATTGATATGGGTATGNCCTATNAAGAAT
TATCAANNTTTGGTAAATTAAGAAAAGTCAATCGTTGTGGTCCAGTTTCAATGTTTGAAC
GTTTGGTTGCAGATTGGGCACATTTAGAACCATCAGTTGNGGCTNAAAAAGTTAAACGTT
TCTTTTATTANTANGCAATCAATCGTCATAAATTAACAACTTTAACACCATCCTATCATG
CTGAAGGTTATTCTCCTGATGATAATCGTTATGATCATCGTCAATTCCTTTACAATTCAA
AATGGGATGTTC
Length of connected seq. 1022
Full length Seq ID -
Full length Seq. -
Length of full length seq. -