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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CHF324
Library CH
(Link to library)
Clone ID CHF324
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15318-1|Contig-U15810-1
Original site URL
Representative seq. ID CHF324P
(Link to Original site)
Representative DNA sequence 
>CHF324 (CHF324Q) /CSM/CH/CHF3-A/CHF324Q.Seq.d/
GAAAAAAAAATTTTTAATTTTTCATTTCATACCAAAAAAAATAGATAACAAAATTAAAAA
TAAAATGACAATAACAAGTCCAGTAATTAAAACGCCACCATTAAATTCAGAGATTAGACT
TGAATCAAATTTAACAGTTGAATCAGGTGATATTGAAATTAATGAAAAAGATATTGTAAA
TGCATCGGAAGTAATAGTAATTTTAGATGCAGGATCGCAATATAGTAAAGTGATTGATAG
AAGAGTTAGAGAATTAAATGTTGCATCAGAAATTCATCCATTAAATATAGATTTATTAGA
ATTAATTAAGATTAAATCAAAATCAGGATCAACTATTAAAGGTATAATTATCTCTGGTGG
TCCAGAGAGTGTCTATGGTGAGAATGCACCAAAATTCGATAAATCATTATTCTCTGAGAA
ATTAAATTTACCAATATTTGGTATTTGTTATGGTATGCAATTAATGAATTATATATTTGG
TGGAAAGGTTGAAAGTAATTCACAACGTGAAGATGGTGTGCACAATATTGAGATTTTAAA
AGATGAAAATCAACAACTTGTTTCAAAACTATTTAAAAATTTAAATCAAACCGAGCAAGT
TTTATTAACACATGGTGATAGTGTTAXXXXXXXXXXTGGCCCAGGTTTAGCAATTCGTAT
CATTTGTGCTGATGAACCTTATCTAGTGAATTATGATTTTACAAATAATGTCGTGCAATA
TTTAGTAACCGGTGAAGCTTCCTCAGAGTTGGAATCAGAAGTTAAAATTAAAATTGATAA
ACAATTAACAGAGATGAAATGTAAACGTCAAGATAAAATTACAATTAAACCAGTTTTATT
ACCAATTCAAACTGTTGGTGTTCAAGGTGATGGTAGAACTTATTCTTATTTATTAGGTCT
TTATAGTTCAGAGAATTCAACAATCGACCAAATTCCTTGGTCTTATATTTTCAATTTAGC
TAGAACTATACCAAAGATTTGTCATAATATAAATAGAGTGGTTTTCATTTTCTCTCAAAA
TGCTACTAAACATACAAATATTAAAGTTTCAAATGAACCAGTTAAACATATCACACCAAC
TCGTTTAACTCCTGATGTTATTAAACAACTTCAACATGCTGATTCAATAGTTTCAGAACA
ACTCTATAAATATAATTTAATTAAATCACTATCCCAAGTTCCCGTTGTATCATTACCAAT
AGATTTCGGTGTTACAGGTAATAGATCAATCGCAATTCGTACTTTTATTACAAATGATTT
CATGACTGGTGTCCCTGCGATTCCTGGTAAAAATATTAGTTTCGATTGTTTACAAGAAAT
TACAAATAATATTTTAACTTCTGTAAAGGTATTTCAAAAGTTTTATTGATGACTTCAAAA
C
sequence update 2002.10.25
Translated Amino Acid sequence 
KKKFLIFHFIPKKIDNKIKNKMTITSPVIKTPPLNSEIRLESNLTVESGDIEINEKDIVN
ASEVIVILDAGSQYSKVIDRRVRELNVASEIHPLNIDLLELIKIKSKSGSTIKGIIISGG
PESVYGENAPKFDKSLFSEKLNLPIFGICYGMQLMNYIFGGKVESNSQREDGVHNIEILK
DENQQLVSKLFKNLNQTEQVLLTHGDSV---

---GPGLAIRIICADEPYLVNYDFTNNVVQYLVTGEASSELESEVKIKIDKQLTEMKCKR
QDKITIKPVLLPIQTVGVQGDGRTYSYLLGLYSSENSTIDQIPWSYIFNLARTIPKICHN
INRVVFIFSQNATKHTNIKVSNEPVKHITPTRLTPDVIKQLQHADSIVSEQLYKYNLIKS
LSQVPVVSLPIDFGVTGNRSIAIRTFITNDFMTGVPAIPGKNISFDCLQEITNNILTSVK
VFQKFY**lqn
Translated Amino Acid sequence (All Frames) 
Frame A:
ekkifnfsfhtkknr*qn*k*ndnnkssn*natikfrd*t*ikfns*ir*y*n**kryck
cigsnsnfrcriai**sd**ks*rikccirnssikyrfirin*d*ikiriny*rynylww
sreclw*ectkir*iiil*eikftniwyllwyainelyiwwkg*k*ftt*rwcaqy*dfk
r*ksttcfkti*kfksnrasfintw**c---

---wprfsnsyhlc**tlssel*fyk*craifsnr*sflrvgirs*n*n**tinrdem*t
sr*nyn*tsfitnsncwcsr*w*nlflfirsl*frefnnrpnslvlyfqfs*nytkdls*
yk*sgfhflskcy*tyky*sfk*ts*tyhtnsfns*cy*ttstc*fnsfrttl*i*fn*i
tipssrciitnrfrcyr**inrnsyfyyk*fhdwcpcdsw*ky*frlftrnyk*yfnfck
giskvllmtsk

Frame B:
KKKFLIFHFIPKKIDNKIKNKMTITSPVIKTPPLNSEIRLESNLTVESGDIEINEKDIVN
ASEVIVILDAGSQYSKVIDRRVRELNVASEIHPLNIDLLELIKIKSKSGSTIKGIIISGG
PESVYGENAPKFDKSLFSEKLNLPIFGICYGMQLMNYIFGGKVESNSQREDGVHNIEILK
DENQQLVSKLFKNLNQTEQVLLTHGDSV---

---GPGLAIRIICADEPYLVNYDFTNNVVQYLVTGEASSELESEVKIKIDKQLTEMKCKR
QDKITIKPVLLPIQTVGVQGDGRTYSYLLGLYSSENSTIDQIPWSYIFNLARTIPKICHN
INRVVFIFSQNATKHTNIKVSNEPVKHITPTRLTPDVIKQLQHADSIVSEQLYKYNLIKS
LSQVPVVSLPIDFGVTGNRSIAIRTFITNDFMTGVPAIPGKNISFDCLQEITNNILTSVK
VFQKFY**lqn

Frame C:
kknf*ffisyqkk*itklkik*q*qvq*lkrhh*iqrldlnqi*qlnqvilklmkkil*m
hrk***f*mqdrnivk*lieelen*mlhqkfih*i*iy*n*lrlnqnqdqllkv*lslvv
qrvsmvrmhqnsinhyslrn*iyqylvfvmvcn**iiylverlkvihnvkmvctilrf*k
mkinnlfqnylki*ikpskfy*hmvivl---

---aqv*qfvsfvlmnli**imilqimscni**pvklpqswnqklklklinn*qr*nvnv
kiklqlnqfyyqfkllvfkvmveliliy*vfivqriqqstkflglifsi*lelyqrfvii
*iewfsfslkmllniqilkfqmnqlnishqlv*llmllnnfnmliq*fqnnsinii*lnh
ypkfplyhyq*isvlqvidqsqfvlllqmis*lvslrflvkilvsivykklqiif*ll*r
yfksfiddfk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHF324 (CHF324Q) /CSM/CH/CHF3-A/CHF324Q.Seq.d/                       2597   0.0
CHE611 (CHE611Q) /CSM/CH/CHE6-A/CHE611Q.Seq.d/                       1461   0.0
VHA621 (VHA621Q) /CSM/VH/VHA6-A/VHA621Q.Seq.d/                       1439   0.0
VHJ185 (VHJ185Q) /CSM/VH/VHJ1-D/VHJ185Q.Seq.d/                       1392   0.0
SFL409 (SFL409Q) /CSM/SF/SFL4-A/SFL409Q.Seq.d/                       1287   0.0
CFJ333 (CFJ333Q) /CSM/CF/CFJ3-B/CFJ333Q.Seq.d/                       1195   0.0
VFL146 (VFL146Q) /CSM/VF/VFL1-B/VFL146Q.Seq.d/                       1178   0.0
VFK690 (VFK690Q) /CSM/VF/VFK6-D/VFK690Q.Seq.d/                       1168   0.0
AFL647 (AFL647Q) /CSM/AF/AFL6-B/AFL647Q.Seq.d/                       1164   0.0
CHF121 (CHF121Q) /CSM/CH/CHF1-A/CHF121Q.Seq.d/                       1160   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M64282|M64282.1 Dictyostelium discoideum GMP synthetase mRNA, complete cds.  1394   0.0    2
AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.    34   2e-14  28
AE017263|AE017263.1 Mesoplasma florum L1 complete genome.          38   1e-13  34
AP006628|AP006628.1 Onion yellows phytoplasma OY-M DNA, complete genome.    42   5e-13  33
AF538053|AF538053.1 Monosiga brevicollis mitochondrion, complete genome.    38   5e-11  14
AC117176|AC117176.2 Dictyostelium discoideum chromosome 2 map 5018074-5200947 strain AX4, complete sequence.    34   1e-10  21
AB063521|AB063521.2 Wigglesworthia brevipalpis DNA, complete genome, section 1/2.    34   3e-10  23
AL445565|AL445565.1 Mycoplasma pulmonis (strain UAB CTIP) complete genome; segment 3/3.    36   2e-09  24
AF250284|AF250284.1 Amsacta moorei entomopoxvirus, complete genome.    36   2e-09  19
AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.    40   3e-09  25
dna update 2004. 9.27
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AK049755_1(AK049755|pid:none) Mus musculus 12 days embryo spinal...   159   2e-37
(Q3THK7) RecName: Full=GMP synthase [glutamine-hydrolyzing];    ...   159   2e-37
AK168239_1(AK168239|pid:none) Mus musculus TIB-55 BB88 cDNA, RIK...   159   2e-37
BC111273_1(BC111273|pid:none) Bos taurus guanine monphosphate sy...   158   5e-37
AK302148_1(AK302148|pid:none) Homo sapiens cDNA FLJ57661 complet...   157   6e-37
CU570794_4(CU570794|pid:none) Zebrafish DNA sequence from clone ...   157   6e-37
AK223399_1(AK223399|pid:none) Homo sapiens mRNA for guanine mono...   157   8e-37
AK146654_1(AK146654|pid:none) Mus musculus 16 days embryo heart ...   155   3e-36
AM502240_11(AM502240|pid:none) Leishmania infantum chromosome 22.     152   3e-35
AJ720923_1(AJ720923|pid:none) Gallus gallus mRNA for hypothetica...   151   6e-35
protein update 2009. 3.31
PSORT 

 psg: 0.93  gvh: 0.37  alm: 0.41  top: 0.57  tms: 0.00  mit: 0.25  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 28.0 %: cytoplasmic
	 20.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for CHF324 is nuc
5' end seq. ID CHF324F
5' end seq. 
>CHF324F.Seq
GAAAAAAAAATTTTTAATTTTTCATTTCATACCAAAAAAAATAGATAACAAAATTAAAAA
TAAAATGACAATAACAAGTCCAGTAATTAAAACGCCACCATTAAATTCAGAGATTAGACT
TGAATCAAATTTAACAGTTGAATCAGGTGATATTGAAATTAATGAAAAAGATATTGTAAA
TGCATCGGAAGTAATAGTAATTTTAGATGCAGGATCGCAATATAGTAAAGTGATTGATAG
AAGAGTTAGAGAATTAAATGTTGCATCAGAAATTCATCCATTAAATATAGATTTATTAGA
ATTAATTAAGATTAAATCAAAATCAGGATCAACTATTAAAGGTATAATTATCTCTGGTGG
TCCAGAGAGTGTCTATGGTGAGAATGCACCAAAATTCGATAAATCATTATTCTCTGAGAA
ATTAAATTTACCAATATTTGGTATTTGTTATGGTATGCAATTAATGAATTATATATTTGG
TGGAAAGGTTGAAAGTAATTCACAACGTGAAGATGGTGTGCACAATATTGAGATTTTAAA
AGATGAAAATCAACAACTTGTTTCAAAACTATTTAAAAATTTAAATCAAACCGAGCAAGT
TTTATTAACACATGGTGATAGTGTTANNNNNNNNNN
Length of 5' end seq. 636
3' end seq. ID CHF324Z
3' end seq. 
>CHF324Z.Seq
NNNNNNNNNNTGGCCCAGGTTTAGCAATTCGTATCATTTGTGCTGATGAACCTTATCTAG
TGAATTATGATTTTACAAATAATGTCGTGCAATATTTAGTAACCGGTGAAGCTTCCTCAG
AGTTGGAATCAGAAGTTAAAATTAAAATTGATAAACAATTAACAGAGATGAAATGTAAAC
GTCAAGATAAAATTACAATTAAACCAGTTTTATTACCAATTCAAACTGTTGGTGTTCAAG
GTGATGGTAGAACTTATTCTTATTTATTAGGTCTTTATAGTTCAGAGAATTCAACAATCG
ACCAAATTCCTTGGTCTTATATTTTCAATTTAGCTAGAACTATACCAAAGATTTGTCATA
ATATAAATAGAGTGGTTTTCATTTTCTCTCAAAATGCTACTAAACATACAAATATTAAAG
TTTCAAATGAACCAGTTAAACATATCACACCAACTCGTTTAACTCCTGATGTTATTAAAC
AACTTCAACATGCTGATTCAATAGTTTCAGAACAACTCTATAAATATAATTTAATTAAAT
CACTATCCCAAGTTCCCGTTGTATCATTACCAATAGATTTCGGTGTTACAGGTAATAGAT
CAATCGCAATTCGTACTTTTATTACAAATGATTTCATGACTGGTGTCCCTGCGATTCCTG
GTAAAAATATTAGTTTCGATTGTTTACAAGAAATTACAAATAATATTTTAACTTCTGTAA
AGGTATTTCAAAAGTTTTATTGATGACTTCAAAAC
Length of 3' end seq. 755
Connected seq. ID CHF324P
Connected seq. 
>CHF324P.Seq
GAAAAAAAAATTTTTAATTTTTCATTTCATACCAAAAAAAATAGATAACAAAATTAAAAA
TAAAATGACAATAACAAGTCCAGTAATTAAAACGCCACCATTAAATTCAGAGATTAGACT
TGAATCAAATTTAACAGTTGAATCAGGTGATATTGAAATTAATGAAAAAGATATTGTAAA
TGCATCGGAAGTAATAGTAATTTTAGATGCAGGATCGCAATATAGTAAAGTGATTGATAG
AAGAGTTAGAGAATTAAATGTTGCATCAGAAATTCATCCATTAAATATAGATTTATTAGA
ATTAATTAAGATTAAATCAAAATCAGGATCAACTATTAAAGGTATAATTATCTCTGGTGG
TCCAGAGAGTGTCTATGGTGAGAATGCACCAAAATTCGATAAATCATTATTCTCTGAGAA
ATTAAATTTACCAATATTTGGTATTTGTTATGGTATGCAATTAATGAATTATATATTTGG
TGGAAAGGTTGAAAGTAATTCACAACGTGAAGATGGTGTGCACAATATTGAGATTTTAAA
AGATGAAAATCAACAACTTGTTTCAAAACTATTTAAAAATTTAAATCAAACCGAGCAAGT
TTTATTAACACATGGTGATAGTGTTA----------TGGCCCAGGTTTAGCAATTCGTAT
CATTTGTGCTGATGAACCTTATCTAGTGAATTATGATTTTACAAATAATGTCGTGCAATA
TTTAGTAACCGGTGAAGCTTCCTCAGAGTTGGAATCAGAAGTTAAAATTAAAATTGATAA
ACAATTAACAGAGATGAAATGTAAACGTCAAGATAAAATTACAATTAAACCAGTTTTATT
ACCAATTCAAACTGTTGGTGTTCAAGGTGATGGTAGAACTTATTCTTATTTATTAGGTCT
TTATAGTTCAGAGAATTCAACAATCGACCAAATTCCTTGGTCTTATATTTTCAATTTAGC
TAGAACTATACCAAAGATTTGTCATAATATAAATAGAGTGGTTTTCATTTTCTCTCAAAA
TGCTACTAAACATACAAATATTAAAGTTTCAAATGAACCAGTTAAACATATCACACCAAC
TCGTTTAACTCCTGATGTTATTAAACAACTTCAACATGCTGATTCAATAGTTTCAGAACA
ACTCTATAAATATAATTTAATTAAATCACTATCCCAAGTTCCCGTTGTATCATTACCAAT
AGATTTCGGTGTTACAGGTAATAGATCAATCGCAATTCGTACTTTTATTACAAATGATTT
CATGACTGGTGTCCCTGCGATTCCTGGTAAAAATATTAGTTTCGATTGTTTACAAGAAAT
TACAAATAATATTTTAACTTCTGTAAAGGTATTTCAAAAGTTTTATTGATGACTTCAAAA
C
Length of connected seq. 1371
Full length Seq ID -
Full length Seq. -
Length of full length seq. -