>CHF272 (CHF272Q) /CSM/CH/CHF2-C/CHF272Q.Seq.d/
XXXXXXXXXXTGTTGGCCTACTGGAAATAAAACAGNAACNTTNAATATTAATAATTANAN
ACNTTAAAAAAAAAGAAATAATAATAATTAAGAATAAAAAAAAAAAAACTATAATAACAT
TTCAATT

---VGLLEIKQXXXILIIXXXKKKEIIIIKNKKKKTIITFQ

Frame A:
---VGLLEIKQXXXILIIXXXKKKEIIIIKNKKKKTIITFQ


Frame B:
---laywk*nxnxxy**lxtlkkkk***lrikkkkl**hfn


Frame C:
---wptgnktxtxninnxxx*kkrnnnn*e*kkknynnisi

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHF272 (CHF272Q) /CSM/CH/CHF2-C/CHF272Q.Seq.d/                         80   8e-15
SFG108 (SFG108Q) /CSM/SF/SFG1-A/SFG108Q.Seq.d/                         58   3e-08
SSI889 (SSI889Q) /CSM/SS/SSI8-D/SSI889Q.Seq.d/                         48   3e-05
SHH168 (SHH168Q) /CSM/SH/SHH1-C/SHH168Q.Seq.d/                         48   3e-05
SFI174 (SFI174Q) /CSM/SF/SFI1-D/SFI174Q.Seq.d/                         48   3e-05
SFH503 (SFH503Q) /CSM/SF/SFH5-A/SFH503Q.Seq.d/                         48   3e-05
SFE863 (SFE863Q) /CSM/SF/SFE8-C/SFE863Q.Seq.d/                         48   3e-05
SFD628 (SFD628Q) /CSM/SF/SFD6-B/SFD628Q.Seq.d/                         48   3e-05
SFD182 (SFD182Q) /CSM/SF/SFD1-D/SFD182Q.Seq.d/                         48   3e-05
CHS114 (CHS114Q) /CSM/CH/CHS1-A/CHS114Q.Seq.d/                         48   3e-05

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M26238|M26238.1 D.discoideum spore coat protein SP70 gene, complete cds.    48   2e-15  3
M98546|M98546.1 Dictyostelium discoideum spore coat protein (cotB) gene, 5' end and promoter region.    48   2e-15  3
AC115584|AC115584.2 Dictyostelium discoideum chromosome 2 map complement(3622543-3568102) strain AX4, complete sequence.    42   9e-10  3
AJ279060|AJ279060.1 Dictyostelium discoideum gdt1 gene for hypothetical protein, strain  AX2.    32   0.013  4
AL924428|AL924428.1 Danio rerio EST sequence, clone 139-F08-1.     40   0.014  2
AL645792|AL645792.7 Zebrafish DNA sequence from clone RP71-1D10 in linkage group 14  Contains two novel genes similar to MATR3 (matrin 3), a novel gene  similar to PDE6A (cGMP-specific phosphodiesterase 6A alpha (rod)), a novel gene similar to PERC (PGC-1-related estrogen receptor alpha  coactivator) and two CpG islands.    32   0.017  6
BX000521|BX000521.7 Zebrafish DNA sequence from clone CH211-137C8 in linkage group 4.    36   0.030  4
AJ000992|AJ000992.1 Dictyostelium discoideum gdt1 gene.            32   0.030  4
V00701|V00701.1 Yeast mitochondrial HpaII-fragment 2.              28   0.031  4
AL744300|AL744300.1 Danio rerio genomic clone DKEY-8F24, genomic survey sequence.    30   0.047  3

 ***** No hits found ******

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3101297
Number of Hits to DB: 50,276,325

 psg: 0.88  gvh: 0.28  alm: 0.42  top: 0.73  tms: 0.00  mit: 0.13  mip: 0.00
 nuc: 0.16  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: nuclear
	 28.0 %: cytoplasmic
	 12.0 %: cytoskeletal
	  4.0 %: Golgi

>> prediction for CHF272 is nuc

>CHF272Z.Seq
NNNNNNNNNNTGTTGGCCTACTGGAAATAAAACAGNAACNTTNAATATTAATAATTANAN
ACNTTAAAAAAAAAGAAATAATAATAATTAAGAATAAAAAAAAAAAAACTATAATAACAT
TTCAATT