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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFF679
Library AF
(Link to library)
Clone ID AFF679
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15333-1
Original site URL
Representative seq. ID AFF679P
(Link to Original site)
Representative DNA sequence 
>AFF679 (AFF679Q) /CSM/AF/AFF6-D/AFF679Q.Seq.d/
ATATATTTAAATAATAATGCAATCACTTGCACGTTTATCTCGTACCTCTTTGGTTCAAAA
GGGTCTTGTTCCACAAACCATTAAAAACTATTCATCAGTTTCACAAGCTGAAATTGATAA
TGAACCAAGATTTTTAGAATGTTTCAAAACTTTCTTTGATAAAGCAGCTGGTTTAACCAA
TTTAAAACCAGGTGTTTTAAATAATATGAAAGAATGTAATGTTGCTCTCCGTGTTGAATT
CCCAATCAAGAATGAACATGGTGATGTTGATATTATTGCAGGTTATCGTGCACAACATTC
ACACCATCGTTTACCATGCAAAGGTGGTATTCGTTTCAGTGAAGAAGTAGATCTCCAAGA
AGTTATGGCCTTAGCTTCATTGATGACTTATAAATGTGCTGTCGTCGATGTTCCATTTGG
TGGTGCTAAAGGTGGTGTCCGTATCGACCCAAAGAAATACACTGTCGCCCAACGTGAAAA
GATCACTCGTGCCTACACTCTCCTCTTATGTCAAAAGAATTTCATCGGACCAGGTGTCGA
TGTACCAGCTCCAGATATGGGTACAGGTGAACAAGAAATGGCATGGATTCGTGATACCTA
TCAAGCTTTCAACACCAACGATGTTGATTCXXXXXXXXXXACGGTACTTTCATTGATTTC
CCAGGTGCCACCAACATTGTCGACTCTGTCAAAGCCTTAGAGATCCCATGTGATATCTTA
ATCCCAGCTGCCCTCGAAAAACAAATCCACATTGGTAACGTCGCCGATATTCAAGCTAAA
TTAATTGGTGAAGCTGCCAACGGTCCAATGACCCCACGTGCTGACCAAATCCTCCTCAAT
AGAGGTCATGTCATCATTCCAGATCTCCTCTTAAATGCTGGTGGTGTCACTGTATCATAC
TTTGAATGGTTAAAGAATCTTTCTCACGTTCGTTTCGGTCGTTTGAACAAGAAATGGGAA
GAATCCTCAAAGAAATTATTATTAGAATTTGTTGAATCAACCGTCAACAAGAAATTATCA
GAAGCTGAAAGAAGCTTAATCATCCATGGTGCTGATGAAATCGATATCGTTCGTTCAGGT
CTCGAAGATACCATGCAAAATGCTTGTGCTGAAACCAGAAAGACTGCAAATGAAAAGAAC
ACTGATTACCGTTCTGCCGCTTTATACAATGCTATCATGAAGNATTAAAGCTGTNCTATG
AATTNNCTGGTAA
sequence update 2001. 6. 2
Translated Amino Acid sequence 
yi*IIMQSLARLSRTSLVQKGLVPQTIKNYSSVSQAEIDNEPRFLECFKTFFDKAAGLTN
LKPGVLNNMKECNVALRVEFPIKNEHGDVDIIAGYRAQHSHHRLPCKGGIRFSEEVDLQE
VMALASLMTYKCAVVDVPFGGAKGGVRIDPKKYTVAQREKITRAYTLLLCQKNFIGPGVD
VPAPDMGTGEQEMAWIRDTYQAFNTNDVD---

---GTFIDFPGATNIVDSVKALEIPCDILIPAALEKQIHIGNVADIQAKLIGEAANGPMT
PRADQILLNRGHVIIPDLLLNAGGVTVSYFEWLKNLSHVRFGRLNKKWEESSKKLLLEFV
ESTVNKKLSEAERSLIIHGADEIDIVRSGLEDTMQNACAETRKTANEKNTDYRSAALYNA
IMKX*scxmnxlv
Translated Amino Acid sequence (All Frames) 
Frame A:
iylnnnaitctfisylfgskgscstnh*klfisfts*n***tkifrmfqnfl**sswfnq
fktrcfk*yerm*ccspc*ipnqe*tw*c*yycrlscttftpsftmqrwysfq*rsrspr
syglsfiddl*mccrrcsiwwc*rwcpyrpkeihcrpt*kdhsclhsplmskefhrtrcr
ctssrygyr*trngmds*ylssfqhqrc*f---

---tvlslisqvpptlstlskp*rshvis*sqlpsknkstlvtspifkln*lvklptvq*
phvltksssievmssfqiss*mlvvslyhtlng*rifltfvsvv*trngknpqrnyy*nl
lnqpstrnyqklkea*ssmvlmksisfvqvskipckmlvlkperlqmkrtlitvlplytm
ls*xikavl*ixw*

Frame B:
yi*IIMQSLARLSRTSLVQKGLVPQTIKNYSSVSQAEIDNEPRFLECFKTFFDKAAGLTN
LKPGVLNNMKECNVALRVEFPIKNEHGDVDIIAGYRAQHSHHRLPCKGGIRFSEEVDLQE
VMALASLMTYKCAVVDVPFGGAKGGVRIDPKKYTVAQREKITRAYTLLLCQKNFIGPGVD
VPAPDMGTGEQEMAWIRDTYQAFNTNDVD---

---ryfh*fprchqhcrlcqslrdpm*ylnpscprktnphw*rrryss*inw*scqrsnd
ptc*pnppq*rschhsrsplkcwwchciil*mvkesfsrsfrsfeqemgrilkeiiiric
*inrqqeiirs*kklnhpwc**nryrsfrsrryhakclc*nqkdck*keh*lpfcrfiqc
yhexlklxyexxg

Frame C:
ifk**cnhlhvylvplwfkrvlfhkplktihqfhklklimnqdf*nvsklslikqlv*pi
*nqvf*ii*knvmllsvlnsqsrmnmvmlillqvivhnihtivyhakvvfvsvkk*iskk
lwp*lh**linvlssmfhlvvlkvvsvstqrntlspnvkrslvptlssyvkrissdqvsm
yqlqiwvqvnkkwhgfvipiklstptmli---

---GTFIDFPGATNIVDSVKALEIPCDILIPAALEKQIHIGNVADIQAKLIGEAANGPMT
PRADQILLNRGHVIIPDLLLNAGGVTVSYFEWLKNLSHVRFGRLNKKWEESSKKLLLEFV
ESTVNKKLSEAERSLIIHGADEIDIVRSGLEDTMQNACAETRKTANEKNTDYRSAALYNA
IMKX*scxmnxlv
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFF679 (AFF679Q) /CSM/AF/AFF6-D/AFF679Q.Seq.d/                       2321   0.0
VFD217 (VFD217Q) /CSM/VF/VFD2-A/VFD217Q.Seq.d/                       1249   0.0
SFJ586 (SFJ586Q) /CSM/SF/SFJ5-D/SFJ586Q.Seq.d/                       1249   0.0
SFG859 (SFG859Q) /CSM/SF/SFG8-C/SFG859Q.Seq.d/                       1249   0.0
SFG811 (SFG811Q) /CSM/SF/SFG8-A/SFG811Q.Seq.d/                       1249   0.0
SFF671 (SFF671Q) /CSM/SF/SFF6-C/SFF671Q.Seq.d/                       1249   0.0
SFF236 (SFF236Q) /CSM/SF/SFF2-B/SFF236Q.Seq.d/                       1249   0.0
CFG496 (CFG496Q) /CSM/CF/CFG4-D/CFG496Q.Seq.d/                       1249   0.0
CFF690 (CFF690Q) /CSM/CF/CFF6-D/CFF690Q.Seq.d/                       1249   0.0
AFN728 (AFN728Q) /CSM/AF/AFN7-B/AFN728Q.Seq.d/                       1249   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

CD421566|CD421566.1 ku85h05.y1 Strongyloides ratti PA female naive pAMP1 v1PROTEIN. [1] ;, mRNA sequence.    80   4e-24  2
CD525630|CD525630.1 kw16f01.y1 Strongyloides ratti PA female naive pAMP1 v1 Strongyloides ratti cDNA 5' similar to TR:Q23621 Q23621 ZK829.4 PROTEIN. [1] ;, mRNA sequence.    78   1e-23  2
CD420727|CD420727.1 ku90g10.y1 Strongyloides ratti PA female naive pAMP1 v1 Strongyloides ratti cDNA 5' similar to TR:Q23621 Q23621 ZK829.4 PROTEIN. [1] ;, mRNA sequence.    74   6e-23  2
CD525857|CD525857.1 kw26g12.y1 Strongyloides ratti PA female naive pAMP1 v1 Strongyloides ratti cDNA 5' similar to TR:Q23621 Q23621 ZK829.4 PROTEIN. [1] ;, mRNA sequence.    74   2e-22  2
CD524962|CD524962.1 kw11g06.y1 Strongyloides ratti PA female naive pAMP1 v1 Strongyloides ratti cDNA 5' similar to TR:Q23621 Q23621 ZK829.4 PROTEIN. [1] ;, mRNA sequence.    72   5e-22  2
BM880231|BM880231.1 ku06h09.y1 Strongyloides ratti PA female naive pAMP1 v1 Strongyloides ratti cDNA 5' similar to TR:Q23621 Q23621 ZK829.4 PROTEIN. [1] ;, mRNA sequence.    72   6e-22  2
BI502159|BI502159.1 kt86a12.y1 Strongyloides ratti L2 pAMP1 v1 Chiapelli McCarter Strongyloides ratti cDNA 5' similar to TR:Q23621 Q23621 ZK829.4 PROTEIN. [1] ;, mRNA sequence.    72   7e-22  2
BM879960|BM879960.1 ku03c02.y1 Strongyloides ratti PA female naive pAMP1 v1 Strongyloides ratti cDNA 5' similar to TR:O02375 O02375 PUTATIVE GLUTAMATE DEHYDROGENASE. ;, mRNA sequence.    72   7e-22  2
BQ091374|BQ091374.1 ku16e09.y1 Strongyloides ratti L2 pAMP1 v1 Chiapelli McCarter Strongyloides ratti cDNA 5' similar to TR:Q23621 Q23621 ZK829.4 PROTEIN. [1] ;, mRNA sequence.    72   7e-22  2
BQ090776|BQ090776.1 ku20a10.y1 Strongyloides ratti L3 adult pAMP1 v1 Chiapelli McCarter Strongyloides ratti cDNA 5' similar to TR:Q23621 Q23621 ZK829.4 PROTEIN. [1] ;, mRNA sequence.    72   7e-22  2
dna update 2004. 4.25
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54KB7) RecName: Full=Glutamate dehydrogenase, mitochondrial;  ...   417   e-115
BC103336_1(BC103336|pid:none) Bos taurus glutamate dehydrogenase...   209   2e-52
AK089152_1(AK089152|pid:none) Mus musculus 10 days neonate olfac...   207   6e-52
(P26443) RecName: Full=Glutamate dehydrogenase 1, mitochondrial;...   207   6e-52
AY256856_1(AY256856|pid:none) Bos taurus brain glutamate dehydro...   207   7e-52
(P00366) RecName: Full=Glutamate dehydrogenase 1, mitochondrial;...   207   7e-52
AY138843_1(AY138843|pid:none) Bos taurus glutamate dehydrogenase...   206   1e-51
(P10860) RecName: Full=Glutamate dehydrogenase 1, mitochondrial;...   206   1e-51
S03707(S03707;S03704;S29786;S29335;S19282) glutamate dehydrogena...   206   2e-51
BX005174_8(BX005174|pid:none) Zebrafish DNA sequence from clone ...   204   4e-51
protein update 2009. 4.20
PSORT 

 psg: 0.73  gvh: 0.45  alm: 0.36  top: 0.53  tms: 0.00  mit: 0.49  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 84.0 %: mitochondrial
	  8.0 %: nuclear
	  4.0 %: cytoplasmic
	  4.0 %: plasma membrane

>> prediction for AFF679 is mit
5' end seq. ID AFF679F
5' end seq. 
>AFF679F.Seq
ATATATTTAAATAATAATGCAATCACTTGCACGTTTATCTCGTACCTCTTTGGTTCAAAA
GGGTCTTGTTCCACAAACCATTAAAAACTATTCATCAGTTTCACAAGCTGAAATTGATAA
TGAACCAAGATTTTTAGAATGTTTCAAAACTTTCTTTGATAAAGCAGCTGGTTTAACCAA
TTTAAAACCAGGTGTTTTAAATAATATGAAAGAATGTAATGTTGCTCTCCGTGTTGAATT
CCCAATCAAGAATGAACATGGTGATGTTGATATTATTGCAGGTTATCGTGCACAACATTC
ACACCATCGTTTACCATGCAAAGGTGGTATTCGTTTCAGTGAAGAAGTAGATCTCCAAGA
AGTTATGGCCTTAGCTTCATTGATGACTTATAAATGTGCTGTCGTCGATGTTCCATTTGG
TGGTGCTAAAGGTGGTGTCCGTATCGACCCAAAGAAATACACTGTCGCCCAACGTGAAAA
GATCACTCGTGCCTACACTCTCCTCTTATGTCAAAAGAATTTCATCGGACCAGGTGTCGA
TGTACCAGCTCCAGATATGGGTACAGGTGAACAAGAAATGGCATGGATTCGTGATACCTA
TCAAGCTTTCAACACCAACGATGTTGATTC----------
Length of 5' end seq. 630
3' end seq. ID AFF679Z
3' end seq. 
>AFF679Z.Seq
----------ACGGTACTTTCATTGATTTCCCAGGTGCCACCAACATTGTCGACTCTGTC
AAAGCCTTAGAGATCCCATGTGATATCTTAATCCCAGCTGCCCTCGAAAAACAAATCCAC
ATTGGTAACGTCGCCGATATTCAAGCTAAATTAATTGGTGAAGCTGCCAACGGTCCAATG
ACCCCACGTGCTGACCAAATCCTCCTCAATAGAGGTCATGTCATCATTCCAGATCTCCTC
TTAAATGCTGGTGGTGTCACTGTATCATACTTTGAATGGTTAAAGAATCTTTCTCACGTT
CGTTTCGGTCGTTTGAACAAGAAATGGGAAGAATCCTCAAAGAAATTATTATTAGAATTT
GTTGAATCAACCGTCAACAAGAAATTATCAGAAGCTGAAAGAAGCTTAATCATCCATGGT
GCTGATGAAATCGATATCGTTCGTTCAGGTCTCGAAGATACCATGCAAAATGCTTGTGCT
GAAACCAGAAAGACTGCAAATGAAAAGAACACTGATTACCGTTCTGCCGCTTTATACAAT
GCTATCATGAAGNATTAAAGCTGTNCTATGAATTNNCTGGTAA
Length of 3' end seq. 573
Connected seq. ID AFF679P
Connected seq. 
>AFF679P.Seq
ATATATTTAAATAATAATGCAATCACTTGCACGTTTATCTCGTACCTCTTTGGTTCAAAA
GGGTCTTGTTCCACAAACCATTAAAAACTATTCATCAGTTTCACAAGCTGAAATTGATAA
TGAACCAAGATTTTTAGAATGTTTCAAAACTTTCTTTGATAAAGCAGCTGGTTTAACCAA
TTTAAAACCAGGTGTTTTAAATAATATGAAAGAATGTAATGTTGCTCTCCGTGTTGAATT
CCCAATCAAGAATGAACATGGTGATGTTGATATTATTGCAGGTTATCGTGCACAACATTC
ACACCATCGTTTACCATGCAAAGGTGGTATTCGTTTCAGTGAAGAAGTAGATCTCCAAGA
AGTTATGGCCTTAGCTTCATTGATGACTTATAAATGTGCTGTCGTCGATGTTCCATTTGG
TGGTGCTAAAGGTGGTGTCCGTATCGACCCAAAGAAATACACTGTCGCCCAACGTGAAAA
GATCACTCGTGCCTACACTCTCCTCTTATGTCAAAAGAATTTCATCGGACCAGGTGTCGA
TGTACCAGCTCCAGATATGGGTACAGGTGAACAAGAAATGGCATGGATTCGTGATACCTA
TCAAGCTTTCAACACCAACGATGTTGATTC----------ACGGTACTTTCATTGATTTC
CCAGGTGCCACCAACATTGTCGACTCTGTCAAAGCCTTAGAGATCCCATGTGATATCTTA
ATCCCAGCTGCCCTCGAAAAACAAATCCACATTGGTAACGTCGCCGATATTCAAGCTAAA
TTAATTGGTGAAGCTGCCAACGGTCCAATGACCCCACGTGCTGACCAAATCCTCCTCAAT
AGAGGTCATGTCATCATTCCAGATCTCCTCTTAAATGCTGGTGGTGTCACTGTATCATAC
TTTGAATGGTTAAAGAATCTTTCTCACGTTCGTTTCGGTCGTTTGAACAAGAAATGGGAA
GAATCCTCAAAGAAATTATTATTAGAATTTGTTGAATCAACCGTCAACAAGAAATTATCA
GAAGCTGAAAGAAGCTTAATCATCCATGGTGCTGATGAAATCGATATCGTTCGTTCAGGT
CTCGAAGATACCATGCAAAATGCTTGTGCTGAAACCAGAAAGACTGCAAATGAAAAGAAC
ACTGATTACCGTTCTGCCGCTTTATACAATGCTATCATGAAGNATTAAAGCTGTNCTATG
AATTNNCTGGTAA
Length of connected seq. 1203
Full length Seq ID -
Full length Seq. -
Length of full length seq. -