>CHF263 (CHF263Q) /CSM/CH/CHF2-C/CHF263Q.Seq.d/
ATATAAATTACCTTATTACTTAAAGATGACAACAAATAATCCATTTGATAAAGTAAAGGA
TGTATTAAAATCACAAGATCAAACTTATAATTTTTATAATCTTTCAAAACTTCAAGACCC
AAGAATTGAAAAATTACCATACTCCATTAGAATTCTTTTGGAATCAGCAGTTCGTAATTG
TGATAACTTTGAAGTACACGAAAAAGATGTTGAAAATATCTTAAATTGGGAAAACACTGC
AAATAAAGTTGAAATTCCTTTCAAACCAGCAAGAGTATTATTACAAGATTTTACTGGTGT
ACCAGCAGTTGTTGATCTTGCAGCAATGAGAGATGCCATGAAAAGATTAGGTGGTGATCC
AGCAAAAATTAACCCACTTGTTCCAGTTGATTTAGTTATTGATCACTCTGTTCAAGTTGA
TGTATCAAGAACTGTTGATGCTTTAGAGCAAAATCAAAAGATTGAATTCCATAGAAATCA
TGAAAGATTTTCATTTTTAAAATGGGGTGCTCAAGCATTTGATGGATTATTTATTGCTCC
ACCTGGAAGTGGTATTGTACATCAAGTAAATTTAGAGTATATTGCAAGAGAAGTTATGAA
TGGAACTGGTAACCTCTTATATCCGGATAGTGTTNGTTGGTACTGATTCTCATACAACAA
TGXXXXXXXXXXGATAGTATTACCACTGATCATATTTCACCAGCCGGTAACATCAATAGA
AAATCATCAGCCGCTCGTTATTTAGAGTCCAAAGGTGTAAAACCAGAAGATTTTAACACC
TATGGATCTCGTAGAGGTAATGATGAAATTATGGTTAGAGGTACCTTTGCCAATACTCGT
ATTGTTAATAAATTGGCTCCAGCCGTTGGTCCACAAACAACCTATGTACCAACTGGTGAG
TTAATGTTTATTTCAGATGCTGCAGAAAAATATCAATCAGAAGGTCATCAACTCATTGTT
TTGGCTGGTAGTGATTATGGCTCTGGTAGTTCTAGAGATTGGGCTGCCAAAGGTCCATAC
CTTCAAGGAATTAAGTGTGTCATTGCCATCTCATTTGAACGTATTCATAGATCCAATCTC
GTTGGTATGGGTATTATCCCATTGCAATTCCAACCAGGTCAAAATGCCTCTACTTTGGGT
CTCACTGGTAAAGAACAATTCAATATCGAGTTACCAACTGATAAATCCCTCATTAAAACT
GGTCAAACCGTAAAGGTTACCACTAATTGTGGTAAATCTTTTGAAACTATCCTTCGTTTC
GATACTCCAATCGAAGTTGAATATTGGGCAAATAATGGTATCTTATCTTATGTTTTAAGA
AAACTTTTACATTAATTTCTTTTTTTAATATAAAATAAAAAAAAATAATATTATTTAAAA

inylit*r*qqiihlik*rmy*nhkikliifiifqnfktqelknyhtpleffwnqqfviv
itlkytkkmlkis*igktlqiklkflsnqqeyyykillvyqqllilqq*emp*kd*vviq
qklthlfqli*llitlfklmyqellml*skikrlnsieimkdfhf*ngvlkhlmdylllh
levvlyik*i*silqekl*MELVTSYIRIVXVGTDSHTTM---

---DSITTDHISPAGNINRKSSAARYLESKGVKPEDFNTYGSRRGNDEIMVRGTFANTRI
VNKLAPAVGPQTTYVPTGELMFISDAAEKYQSEGHQLIVLAGSDYGSGSSRDWAAKGPYL
QGIKCVIAISFERIHRSNLVGMGIIPLQFQPGQNASTLGLTGKEQFNIELPTDKSLIKTG
QTVKVTTNCGKSFETILRFDTPIEVEYWANNGILSYVLRKLLH*flfli*nkkk*yylk

Frame A:
i*itlllkddnk*si**skgcikitrsnl*fl*sfktsrpkn*kitilh*nsfgisss*l
**l*strkrc*kylklgkhck*s*nsfqtsksiitrfywctssc*scsnerchekirw*s
skn*ptcss*fsy*slcss*ciknc*cfrakskd*ip*ks*kififkmgcssi*wiiycs
twkwyctsskfrvyckrsyewnw*plisg*cxlvliliqq---

---DSITTDHISPAGNINRKSSAARYLESKGVKPEDFNTYGSRRGNDEIMVRGTFANTRI
VNKLAPAVGPQTTYVPTGELMFISDAAEKYQSEGHQLIVLAGSDYGSGSSRDWAAKGPYL
QGIKCVIAISFERIHRSNLVGMGIIPLQFQPGQNASTLGLTGKEQFNIELPTDKSLIKTG
QTVKVTTNCGKSFETILRFDTPIEVEYWANNGILSYVLRKLLH*flfli*nkkk*yylk

Frame B:
yklpyylkmttnnpfdkvkdvlksqdqtynfynlsklqdprieklpysirillesavrnc
dnfevhekdvenilnwentankveipfkparvllqdftgvpavvdlaamrdamkrlggdp
akinplvpvdlvidhsvqvdvsrtvdaleqnqkiefhrnherfsflkwgaqafdglfiap
pgsgivhqvnleyiarevmngtgnllypdsvxwy*fsynn---

---ivlpliifhqpvtsienhqplvi*spkv*nqkiltpmdlvevmmklwlevplpilvl
linwlqplvhkqpmyqlvs*clfqmlqkninqkvinslfwlvvimalvvleiglpkvhtf
kelsvslpshlnvfidpislvwvlshcnsnqvkmpllwvslvknnsissyqlinpslklv
kp*rlplivvnllklsfvsilqsklnigqimvsylmf*enfyinfff*ykikknnii*

Frame C:
inylit*r*qqiihlik*rmy*nhkikliifiifqnfktqelknyhtpleffwnqqfviv
itlkytkkmlkis*igktlqiklkflsnqqeyyykillvyqqllilqq*emp*kd*vviq
qklthlfqli*llitlfklmyqellml*skikrlnsieimkdfhf*ngvlkhlmdylllh
levvlyik*i*silqekl*MELVTSYIRIVXVGTDSHTTM---

---*yyh*syftsr*hq*kiisrslfrvqrcktrrf*hlwis*r***nyg*rylcqysyc
**igssrwstnnlctnw*vnvyfrccrkisirrssthcfgw**lwlw*f*rlgcqrsips
rn*vchchli*tys*iqsrwygyypiaiptrskclyfgshw*rtiqyrvtn**iph*nws
nrkgyh*lw*if*nypsfrysnrs*ilgk*wylilcfkktftlisffnik*kkiilfk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHQ461 (VHQ461Q) /CSM/VH/VHQ4-C/VHQ461Q.Seq.d/                       1356   0.0
VHQ432 (VHQ432Q) /CSM/VH/VHQ4-B/VHQ432Q.Seq.d/                       1356   0.0
VHQ267 (VHQ267Q) /CSM/VH/VHQ2-C/VHQ267Q.Seq.d/                       1356   0.0
VHO844 (VHO844Q) /CSM/VH/VHO8-B/VHO844Q.Seq.d/                       1356   0.0
VHO678 (VHO678Q) /CSM/VH/VHO6-D/VHO678Q.Seq.d/                       1356   0.0
VHK111 (VHK111Q) /CSM/VH/VHK1-A/VHK111Q.Seq.d/                       1356   0.0
VHD137 (VHD137Q) /CSM/VH/VHD1-B/VHD137Q.Seq.d/                       1356   0.0
VHA714 (VHA714Q) /CSM/VH/VHA7-A/VHA714Q.Seq.d/                       1356   0.0
VHA224 (VHA224Q) /CSM/VH/VHA2-A/VHA224Q.Seq.d/                       1356   0.0
SHL826 (SHL826Q) /CSM/SH/SHL8-B/SHL826Q.Seq.d/                       1356   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AF194945|AF194945.1 Nicotiana tabacum cytosolic aconitase (ACO) mRNA, complete cds.    56   5e-31  6
AY250116|AY250116.1 Lycopersicon pennellii aconitase mRNA, partial cds.    60   5e-26  5
AY250115|AY250115.1 Lycopersicon pennellii aconitase mRNA, partial cds.    60   2e-21  5
BE579347|BE579347.1 kq26c09.y1 TBN95TM-SSR Strongyloides stercoralis cDNA 5' similar to WP:ZK455.1 CE03812 PROBABLE ACONITATE HYDRATASE, CYTOPLASMIC ;, mRNA sequence.    64   2e-20  3
BI925204|BI925204.1 EST545093 tomato flower, buds 0-3 mm Lycopersicon esculentum cDNA clone cTOA25H12 5' end, mRNA sequence.    58   5e-19  3
BI930520|BI930520.1 EST550409 tomato flower, 8 mm to preanthesis buds Lycopersicon esculentum cDNA clone cTOC16J14 5' end, mRNA sequence.    58   6e-19  3
CO910019|CO910019.1 BJ02055G09 BJ02 Capsicum annuum cDNA 5', mRNA sequence.    58   1e-18  4
CK265172|CK265172.1 EST711250 potato abiotic stress cDNA library Solanum tuberosum cDNA clone POABX20 5' end, mRNA sequence.    58   1e-17  4
AF073507|AF073507.1 Citrus limon aconitase-iron regulated protein 1 (IRP1) mRNA, complete cds.    56   1e-17  7
AY062772|AY062772.1 Arabidopsis thaliana Unknown protein (At4g35830; F4B14.100) mRNA, complete cds.    46   5e-17  6

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54X73) RecName: Full=Probable cytoplasmic aconitate hydratase;...   443   e-123
AB236788_1(AB236788|pid:none) Trifolium pratense RNA for putativ...   291   3e-77
AP008209_256(AP008209|pid:none) Oryza sativa (japonica cultivar-...   290   8e-77
BT060820_1(BT060820|pid:none) Zea mays full-length cDNA clone ZM...   288   3e-76
BT060665_1(BT060665|pid:none) Zea mays full-length cDNA clone ZM...   287   5e-76
(Q23500) RecName: Full=Probable cytoplasmic aconitate hydratase;...   287   5e-76
(P49608) RecName: Full=Aconitate hydratase, cytoplasmic;        ...   284   4e-75
EU816775_1(EU816775|pid:none) Perkinsus marinus aconitase-1 mRNA...   283   7e-75
DQ294230_1(DQ294230|pid:none) Glossina morsitans morsitans TC165...   283   7e-75
(Q9SIB9) RecName: Full=Aconitate hydratase 2, mitochondrial;    ...   283   1e-74

 psg: 0.77  gvh: 0.26  alm: 0.47  top: 0.53  tms: 0.00  mit: 0.25  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 28.0 %: cytoplasmic
	 20.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: extracellular, including cell wall

>> prediction for CHF263 is nuc

>CHF263F.Seq
ATATAAATTACCTTATTACTTAAAGATGACAACAAATAATCCATTTGATAAAGTAAAGGA
TGTATTAAAATCACAAGATCAAACTTATAATTTTTATAATCTTTCAAAACTTCAAGACCC
AAGAATTGAAAAATTACCATACTCCATTAGAATTCTTTTGGAATCAGCAGTTCGTAATTG
TGATAACTTTGAAGTACACGAAAAAGATGTTGAAAATATCTTAAATTGGGAAAACACTGC
AAATAAAGTTGAAATTCCTTTCAAACCAGCAAGAGTATTATTACAAGATTTTACTGGTGT
ACCAGCAGTTGTTGATCTTGCAGCAATGAGAGATGCCATGAAAAGATTAGGTGGTGATCC
AGCAAAAATTAACCCACTTGTTCCAGTTGATTTAGTTATTGATCACTCTGTTCAAGTTGA
TGTATCAAGAACTGTTGATGCTTTAGAGCAAAATCAAAAGATTGAATTCCATAGAAATCA
TGAAAGATTTTCATTTTTAAAATGGGGTGCTCAAGCATTTGATGGATTATTTATTGCTCC
ACCTGGAAGTGGTATTGTACATCAAGTAAATTTAGAGTATATTGCAAGAGAAGTTATGAA
TGGAACTGGTAACCTCTTATATCCGGATAGTGTTNGTTGGTACTGATTCTCATACAACAA
TGNNNNNNNNNN

>CHF263Z.Seq
NNNNNNNNNNGATAGTATTACCACTGATCATATTTCACCAGCCGGTAACATCAATAGAAA
ATCATCAGCCGCTCGTTATTTAGAGTCCAAAGGTGTAAAACCAGAAGATTTTAACACCTA
TGGATCTCGTAGAGGTAATGATGAAATTATGGTTAGAGGTACCTTTGCCAATACTCGTAT
TGTTAATAAATTGGCTCCAGCCGTTGGTCCACAAACAACCTATGTACCAACTGGTGAGTT
AATGTTTATTTCAGATGCTGCAGAAAAATATCAATCAGAAGGTCATCAACTCATTGTTTT
GGCTGGTAGTGATTATGGCTCTGGTAGTTCTAGAGATTGGGCTGCCAAAGGTCCATACCT
TCAAGGAATTAAGTGTGTCATTGCCATCTCATTTGAACGTATTCATAGATCCAATCTCGT
TGGTATGGGTATTATCCCATTGCAATTCCAACCAGGTCAAAATGCCTCTACTTTGGGTCT
CACTGGTAAAGAACAATTCAATATCGAGTTACCAACTGATAAATCCCTCATTAAAACTGG
TCAAACCGTAAAGGTTACCACTAATTGTGGTAAATCTTTTGAAACTATCCTTCGTTTCGA
TACTCCAATCGAAGTTGAATATTGGGCAAATAATGGTATCTTATCTTATGTTTTAAGAAA
ACTTTTACATTAATTTCTTTTTTTAATATAAAATAAAAAAAAATAATATTATTTAAAA

>CHF263P.Seq
ATATAAATTACCTTATTACTTAAAGATGACAACAAATAATCCATTTGATAAAGTAAAGGA
TGTATTAAAATCACAAGATCAAACTTATAATTTTTATAATCTTTCAAAACTTCAAGACCC
AAGAATTGAAAAATTACCATACTCCATTAGAATTCTTTTGGAATCAGCAGTTCGTAATTG
TGATAACTTTGAAGTACACGAAAAAGATGTTGAAAATATCTTAAATTGGGAAAACACTGC
AAATAAAGTTGAAATTCCTTTCAAACCAGCAAGAGTATTATTACAAGATTTTACTGGTGT
ACCAGCAGTTGTTGATCTTGCAGCAATGAGAGATGCCATGAAAAGATTAGGTGGTGATCC
AGCAAAAATTAACCCACTTGTTCCAGTTGATTTAGTTATTGATCACTCTGTTCAAGTTGA
TGTATCAAGAACTGTTGATGCTTTAGAGCAAAATCAAAAGATTGAATTCCATAGAAATCA
TGAAAGATTTTCATTTTTAAAATGGGGTGCTCAAGCATTTGATGGATTATTTATTGCTCC
ACCTGGAAGTGGTATTGTACATCAAGTAAATTTAGAGTATATTGCAAGAGAAGTTATGAA
TGGAACTGGTAACCTCTTATATCCGGATAGTGTTNGTTGGTACTGATTCTCATACAACAA
TG----------GATAGTATTACCACTGATCATATTTCACCAGCCGGTAACATCAATAGA
AAATCATCAGCCGCTCGTTATTTAGAGTCCAAAGGTGTAAAACCAGAAGATTTTAACACC
TATGGATCTCGTAGAGGTAATGATGAAATTATGGTTAGAGGTACCTTTGCCAATACTCGT
ATTGTTAATAAATTGGCTCCAGCCGTTGGTCCACAAACAACCTATGTACCAACTGGTGAG
TTAATGTTTATTTCAGATGCTGCAGAAAAATATCAATCAGAAGGTCATCAACTCATTGTT
TTGGCTGGTAGTGATTATGGCTCTGGTAGTTCTAGAGATTGGGCTGCCAAAGGTCCATAC
CTTCAAGGAATTAAGTGTGTCATTGCCATCTCATTTGAACGTATTCATAGATCCAATCTC
GTTGGTATGGGTATTATCCCATTGCAATTCCAACCAGGTCAAAATGCCTCTACTTTGGGT
CTCACTGGTAAAGAACAATTCAATATCGAGTTACCAACTGATAAATCCCTCATTAAAACT
GGTCAAACCGTAAAGGTTACCACTAATTGTGGTAAATCTTTTGAAACTATCCTTCGTTTC
GATACTCCAATCGAAGTTGAATATTGGGCAAATAATGGTATCTTATCTTATGTTTTAAGA
AAACTTTTACATTAATTTCTTTTTTTAATATAAAATAAAAAAAAATAATATTATTTAAAA