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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CHF248
Library CH
(Link to library)
Clone ID CHF248
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16511-1
Original site URL
Representative seq. ID CHF248P
(Link to Original site)
Representative DNA sequence 
>CHF248 (CHF248Q) /CSM/CH/CHF2-B/CHF248Q.Seq.d/
TGTCAATAGTAATAAATACATTTTTTTCTTTATTTTTTTTTTATTAAATAAAATATATAG
TANCATTAGATTAGTAATAATATAATATAATATTACAAAAAGAAAATTTATTTTTTATCA
AATTTGAGAAAAAAAAAAXXXXXXXXXXAACCAGNTNAACATGCANTNANCAACAACATG
CCAAACCAGCCCAAAATGNTAAACCAGTTCAACATAACGCCCAACAACAACATGCCAAAC
CAGNTCAACATGCCGNTCAACAACAACATGCCAAACCAGCTCAACATGTCGCTCAACAAC
AACAACAACAACACGCCAAACCAGCTGCTCATACTGCTGCCAAACCAGTTCAACATAATG
CCCAACAACAACATGCTAAACCAGCTGCTCATACTGCTGCCAAACCAGTTCAACACAATG
CTGCTCAACAACATGCTAAACCAGCTGCCCATGGCGCCAAACCAGCTGTTAATGCTGCCA
GACCAGTTCAACACAATGCTGCTCAACAACATGCTAAACCAGCTGCTCATACTGCTGCCA
AACCAGTTCAACACAATGCTGCTCAACAACATGCTAAACCAGCTGCCAAACCAGCCAAAT
TAACCAATGCCCAAAAATTACAACAAGAACACCAAAGACAAGAAGCTCTCGCAAAACAAC
AAGCTCGTATTAGACAACAAAATCTTGTCAAACAAGCTGCCCAAAAGAAACAAACCTCTC
AACGTGCTGCCTCTAAAAATCAA
sequence update 2002.10.25
Translated Amino Acid sequence 
sivintffslfffy*ikyivxld***YNIILQKENLFFIKFEKKK---

---TXXTCXXQQHAKPAQNXKPVQHNAQQQHAKPXQHAXQQQHAKPAQHVAQQQQQQHAK
PAAHTAAKPVQHNAQQQHAKPAAHTAAKPVQHNAAQQHAKPAAHGAKPAVNAARPVQHNA
AQQHAKPAAHTAAKPVQHNAAQQHAKPAAKPAKLTNAQKLQQEHQRQEALAKQQARIRQQ
NLVKQAAQKKQTSQRAASKNQ
Translated Amino Acid sequence (All Frames) 
Frame A:
cq***ihfflyfffik*ni*xh*isnnii*yykkkiyflsnlrkkk---

---nqxnmxxxttcqtspkx*tsst*rptttcqtxstcrstttcqtsstcrsttttttrq
tscsyccqtsst*cptttc*tscsyccqtsstqccsttc*tscpwrqtsc*ccqtsstqc
csttc*tscsyccqtsstqccsttc*tscqtsqinqcpkittrtpktrssrkttssy*tt
kscqtscpketnlstccl*ks

Frame B:
vnsnkyifffiffllnkiysxirlvii*ynitkrkfifyqi*ekk---

---TXXTCXXQQHAKPAQNXKPVQHNAQQQHAKPXQHAXQQQHAKPAQHVAQQQQQQHAK
PAAHTAAKPVQHNAQQQHAKPAAHTAAKPVQHNAAQQHAKPAAHGAKPAVNAARPVQHNA
AQQHAKPAAHTAAKPVQHNAAQQHAKPAAKPAKLTNAQKLQQEHQRQEALAKQQARIRQQ
NLVKQAAQKKQTSQRAASKNQ

Frame C:
sivintffslfffy*ikyivxld***YNIILQKENLFFIKFEKKK---

---pxxhaxxnnmpnqpkmxnqfnitpnnnmpnqxnmpxnnnmpnqlnmslnnnnnntpn
qllillpnqfnimpnnnmlnqllillpnqfntmllnnmlnqlpmapnqllmlpdqfntml
lnnmlnqllillpnqfntmllnnmlnqlpnqpn*pmpknynkntkdkklsqnnklvldnk
ilsnklpkrnkplnvlplki
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHF874 (CHF874Q) /CSM/CH/CHF8-D/CHF874Q.Seq.d/                        852   0.0
SLB726 (SLB726Q) /CSM/SL/SLB7-B/SLB726Q.Seq.d/                        852   0.0
SLB567 (SLB567Q) /CSM/SL/SLB5-C/SLB567Q.Seq.d/                        852   0.0
SHJ113 (SHJ113Q) /CSM/SH/SHJ1-A/SHJ113Q.Seq.d/                        852   0.0
SFL469 (SFL469Q) /CSM/SF/SFL4-C/SFL469Q.Seq.d/                        852   0.0
SFJ571 (SFJ571Q) /CSM/SF/SFJ5-C/SFJ571Q.Seq.d/                        852   0.0
SFH246 (SFH246Q) /CSM/SF/SFH2-B/SFH246Q.Seq.d/                        852   0.0
SFG263 (SFG263Q) /CSM/SF/SFG2-C/SFG263Q.Seq.d/                        852   0.0
SFD184 (SFD184Q) /CSM/SF/SFD1-D/SFD184Q.Seq.d/                        852   0.0
SFC815 (SFC815Q) /CSM/SF/SFC8-A/SFC815Q.Seq.d/                        852   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC117075|AC117075.2 Dictyostelium discoideum chromosome 2 map 5201047-5455781 strain AX4, complete sequence.   852   0.0    13
X57149|X57149.1 D.discoideum EB4 gene for prespore vesicle protein.   571   0.0    10
BU714294|BU714294.1 SJAACBF11 Adult SjC 7/94 Schistosoma japonicum cDNA, mRNA sequence.    34   1e-04  3
CR626910|CR626910.2 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone DKEY-204E20.    38   0.013  5
AL928463|AL928463.1 Danio rerio EST sequence, clone 206-A02-1.     28   0.039  4
BX170546|BX170546.1 Danio rerio genomic clone DKEY-171I20, genomic survey sequence.    28   0.064  4
CR376318|CR376318.1 AGENAE Rainbow trout normalized testis library (tcbi) clone  tcbi0013c.d.17, 5prim end (M13R primer).    28   0.11   4
BX163533|BX163533.1 Danio rerio genomic clone DKEY-166F16, genomic survey sequence.    28   0.12   4
CA050958|CA050958.1 ssalrgb526247 mixed_tissue Salmo salar cDNA, mRNA sequence.    36   0.17   3
BB680188|BB680188.1 Mus musculus 12 days embryo female ovary cDNA, RIKEN full-length enriched library, clone:6620403D21, 3' end partial sequence.    48   0.21   1
dna update 2004. 9.27
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P08798) RecName: Full=Prespore vesicle protein;          Short=...   111   2e-23
S28358(S28358;S20090) prespore vesicle protein - slime mold (Dic...   107   4e-22
AE014296_2972(AE014296|pid:none) Drosophila melanogaster chromos...    37   0.61
BT012673_1(BT012673|pid:none) Drosophila melanogaster SD07102 fu...    37   0.61
AY052115_1(AY052115|pid:none) Drosophila melanogaster SD06557 fu...    37   0.61
(Q54G96) RecName: Full=Uncharacterized protein DDB_G0290301;           36   1.0
M93690_1(M93690|pid:none) Anopheles gambiae RT1 retroposon, comp...    36   1.4
AE016877_2135(AE016877|pid:none) Bacillus cereus ATCC 14579, com...    35   1.8
CP000671_202(CP000671|pid:none) Haemophilus influenzae PittEE, c...    35   1.8
CP001176_2174(CP001176|pid:none) Bacillus cereus B4264, complete...    35   1.8
protein update 2009. 3.31
PSORT 

 psg: 0.70  gvh: 0.26  alm: 0.61  top: 0.53  tms: 0.00  mit: 0.20  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 40.0 %: cytoplasmic
	 12.0 %: cytoskeletal
	  4.0 %: mitochondrial

>> prediction for CHF248 is nuc
5' end seq. ID CHF248F
5' end seq. 
>CHF248F.Seq
TGTCAATAGTAATAAATACATTTTTTTCTTTATTTTTTTTTTATTAAATAAAATATATAG
TANCATTAGATTAGTAATAATATAATATAATATTACAAAAAGAAAATTTATTTTTTATCA
AATTTGAGAAAAAAAAAANNNNNNNNNN
Length of 5' end seq. 148
3' end seq. ID CHF248Z
3' end seq. 
>CHF248Z.Seq
NNNNNNNNNNAACCAGNTNAACATGCANTNANCAACAACATGCCAAACCAGCCCAAAATG
NTAAACCAGTTCAACATAACGCCCAACAACAACATGCCAAACCAGNTCAACATGCCGNTC
AACAACAACATGCCAAACCAGCTCAACATGTCGCTCAACAACAACAACAACAACACGCCA
AACCAGCTGCTCATACTGCTGCCAAACCAGTTCAACATAATGCCCAACAACAACATGCTA
AACCAGCTGCTCATACTGCTGCCAAACCAGTTCAACACAATGCTGCTCAACAACATGCTA
AACCAGCTGCCCATGGCGCCAAACCAGCTGTTAATGCTGCCAGACCAGTTCAACACAATG
CTGCTCAACAACATGCTAAACCAGCTGCTCATACTGCTGCCAAACCAGTTCAACACAATG
CTGCTCAACAACATGCTAAACCAGCTGCCAAACCAGCCAAATTAACCAATGCCCAAAAAT
TACAACAAGAACACCAAAGACAAGAAGCTCTCGCAAAACAACAAGCTCGTATTAGACAAC
AAAATCTTGTCAAACAAGCTGCCCAAAAGAAACAAACCTCTCAACGTGCTGCCTCTAAAA
ATCAA
Length of 3' end seq. 605
Connected seq. ID CHF248P
Connected seq. 
>CHF248P.Seq
TGTCAATAGTAATAAATACATTTTTTTCTTTATTTTTTTTTTATTAAATAAAATATATAG
TANCATTAGATTAGTAATAATATAATATAATATTACAAAAAGAAAATTTATTTTTTATCA
AATTTGAGAAAAAAAAAA----------AACCAGNTNAACATGCANTNANCAACAACATG
CCAAACCAGCCCAAAATGNTAAACCAGTTCAACATAACGCCCAACAACAACATGCCAAAC
CAGNTCAACATGCCGNTCAACAACAACATGCCAAACCAGCTCAACATGTCGCTCAACAAC
AACAACAACAACACGCCAAACCAGCTGCTCATACTGCTGCCAAACCAGTTCAACATAATG
CCCAACAACAACATGCTAAACCAGCTGCTCATACTGCTGCCAAACCAGTTCAACACAATG
CTGCTCAACAACATGCTAAACCAGCTGCCCATGGCGCCAAACCAGCTGTTAATGCTGCCA
GACCAGTTCAACACAATGCTGCTCAACAACATGCTAAACCAGCTGCTCATACTGCTGCCA
AACCAGTTCAACACAATGCTGCTCAACAACATGCTAAACCAGCTGCCAAACCAGCCAAAT
TAACCAATGCCCAAAAATTACAACAAGAACACCAAAGACAAGAAGCTCTCGCAAAACAAC
AAGCTCGTATTAGACAACAAAATCTTGTCAAACAAGCTGCCCAAAAGAAACAAACCTCTC
AACGTGCTGCCTCTAAAAATCAA
Length of connected seq. 733
Full length Seq ID -
Full length Seq. -
Length of full length seq. -