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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CHF221
Library CH
(Link to library)
Clone ID CHF221
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16524-1
Original site URL
Representative seq. ID CHF221P
(Link to Original site)
Representative DNA sequence 
>CHF221 (CHF221Q) /CSM/CH/CHF2-A/CHF221Q.Seq.d/
AACTGCAACCTCTGGTAAAGAGACAAACTACGATAATGTTACAGTTATCATTTCAAAGAA
ACCAATCACTGCAGTAATTGATGGAGCCGATAAAGAATTCCCATTAAATACTTTAATAAC
TTTAGATGCTTCAAAATCTTTTGATCCAACAACTACTTCAACAGTTTXXXXXXXXXXCTT
CAGACATTTTAGTGGTATCCAATTCATTGGCAGATGGTATTCTTCAAAGAACTGCAATTG
GTGAAGAACCATTTACAGTTGCAAGTGATAAAGGTTTTTCGATTACAACATTTAAAACTC
TACATATGGAATTAAGTAAGAGTGGTCAATCGATTGGTTTCAAAAAGGATGATGAAGGTC
AATCTCTTGATGGTGAAAAACTTGCTTTCACTCCAAAATTCAGTATTCCATCAAGTGTTT
TATCAAAGAATAAATTAAAACAAACCTCTGAAATCACTGGTAAACTAATTACAGTTCCAT
CAAATATTCATTTCAATCATAAATCTGATCAAACCATTTTCCCAAAAACTGTTTCACTTG
AACTTTTCAATGGTGGCTCTCAATTATCAATCAATGGCCTTAAAGATCAAATTATCATTG
ATATGGGAAAACATTCACCACCAAAAGATAAAGAATTTAAATGTGGTTGGTGGAGCTCCA
ATAAAAAAGAATGGTTAACAGATGGTTGTTCAACCCATAAAGTAGATGGTAATATTCAAT
GTAAATGTAACCATCTTACAGAGTTTGGTTTAATTGAATTTGCAAAATCCTCTCAAAACA
TTTGGATCATTATTGGACCAGTAGTTGGTGGTGTTGTATTTGCAGCTGCAATGGTTGGTA
CTGTTGTATTGGTAAAGAGAAAGAAAAACAAAAACAAAAAGAACAAATCAAAGAAAACCA
AAGAACAAATCAACAAGNAAAAAGNAAGNACAAAAAGAAACA
sequence update 2002.10.25
Translated Amino Acid sequence 
TATSGKETNYDNVTVIISKKPITAVIDGADKEFPLNTLITLDASKSFDPTTTSTV---

---SDILVVSNSLADGILQRTAIGEEPFTVASDKGFSITTFKTLHMELSKSGQSIGFKKD
DEGQSLDGEKLAFTPKFSIPSSVLSKNKLKQTSEITGKLITVPSNIHFNHKSDQTIFPKT
VSLELFNGGSQLSINGLKDQIIIDMGKHSPPKDKEFKCGWWSSNKKEWLTDGCSTHKVDG
NIQCKCNHLTEFGLIEFAKSSQNIWIIIGPVVGGVVFAAAMVGTVVLVKRKKNKNKKNKS
KKTKEQINKXKXXTKRN
Translated Amino Acid sequence (All Frames) 
Frame A:
ncnlw*rdklr*cysyhfketnhcsn*wsr*ripikyfnnfrcfkif*snnyfns---

---lqtf*wypihwqmvffkelqlvknhlqlqvikvfrlqhlklyiwn*vrvvnrlvskr
mmkvnllmvknllslqnsvfhqvfyqrin*nkplkslvn*lqfhqifisiinlikpfsqk
lfhlnfsmvalnyqsmalkiklsliwenihhqkiknlnvvggapikkng*qmvvqpik*m
vifnvnvtilqslv*lnlqnplktfgslldq*lvvlylqlqwlvllyw*rerktktkrtn
qrkpknkstxkkxxqket

Frame B:
TATSGKETNYDNVTVIISKKPITAVIDGADKEFPLNTLITLDASKSFDPTTTSTV---

---frhfsgiqfigrwysskncnw*rtiysck**rffdyni*nstygik*ewsidwfqkg
**rsis*w*ktcfhskiqysikcfike*iktnl*nhw*tnyssikysfqs*i*snhfpkn
cft*tfqwwlsiinqwp*rsnyh*ygktfttkr*ri*mwlvelq*krmvnrwlfnp*srw
*ysm*m*psyrvwfn*ickilskhldhywtsswwccicscngwyccigkekekqkqkeqi
kenqrtnqqxkxkxkkk

Frame C:
lqplvkrqttimlqlsfqrnqslq*lmepiknsh*il**l*mlqnlliqqllqqf---

---SDILVVSNSLADGILQRTAIGEEPFTVASDKGFSITTFKTLHMELSKSGQSIGFKKD
DEGQSLDGEKLAFTPKFSIPSSVLSKNKLKQTSEITGKLITVPSNIHFNHKSDQTIFPKT
VSLELFNGGSQLSINGLKDQIIIDMGKHSPPKDKEFKCGWWSSNKKEWLTDGCSTHKVDG
NIQCKCNHLTEFGLIEFAKSSQNIWIIIGPVVGGVVFAAAMVGTVVLVKRKKNKNKKNKS
KKTKEQINKXKXXTKRN
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHF221 (CHF221Q) /CSM/CH/CHF2-A/CHF221Q.Seq.d/                       1631   0.0
SHL489 (SHL489Q) /CSM/SH/SHL4-D/SHL489Q.Seq.d/                       1332   0.0
SHK593 (SHK593Q) /CSM/SH/SHK5-D/SHK593Q.Seq.d/                       1332   0.0
SHJ824 (SHJ824Q) /CSM/SH/SHJ8-A/SHJ824Q.Seq.d/                       1332   0.0
SHD762 (SHD762Q) /CSM/SH/SHD7-C/SHD762Q.Seq.d/                       1332   0.0
SHD253 (SHD253Q) /CSM/SH/SHD2-C/SHD253Q.Seq.d/                       1332   0.0
SHB414 (SHB414Q) /CSM/SH/SHB4-A/SHB414Q.Seq.d/                       1332   0.0
SFG748 (SFG748Q) /CSM/SF/SFG7-B/SFG748Q.Seq.d/                       1332   0.0
CHS207 (CHS207Q) /CSM/CH/CHS2-A/CHS207Q.Seq.d/                       1332   0.0
CHS192 (CHS192Q) /CSM/CH/CHS1-D/CHS192Q.Seq.d/                       1332   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC006250|AC006250.4 Arabidopsis thaliana chromosome 2 clone T6A13 map mi398, complete sequence.    42   0.008  2
AP006628|AP006628.1 Onion yellows phytoplasma OY-M DNA, complete genome.    34   0.064  18
BX936386|BX936386.4 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone CH211-232P21.    34   0.068  6
BX119904|BX119904.4 Human DNA sequence from clone RP11-232D9 on chromosome X.    36   0.14   11
AJ235273|AJ235273.1 Rickettsia prowazekii strain Madrid E, complete genome; segment 4/4.    34   0.15   9
AP001988|AP001988.5 Homo sapiens genomic DNA, chromosome 11 clone:RP11-76A17, complete sequence.    36   0.17   9
AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.    34   0.18   13
AP004171|AP004171.1 Mycoplasma penetrans HF-2 DNA, complete genome, section 2/5.    30   0.25   12
AC103541|AC103541.6 Homo sapiens BAC clone RP11-818M13 from 4, complete sequence.    48   0.26   1
AC013774|AC013774.3 Homo sapiens clone RP11-10P1, WORKING DRAFT SEQUENCE, 11 unordered pieces.    48   0.26   1
dna update 2004. 9.27
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AK154648_1(AK154648|pid:none) Mus musculus NOD-derived CD11c +ve...    50   1e-04
(Q8R0T6) RecName: Full=Probable G-protein coupled receptor 97; F...    50   1e-04
AK037149_1(AK037149|pid:none) Mus musculus 6 days neonate skin c...    50   1e-04
AB041939_1(AB041939|pid:none) Homo sapiens KPG_006 mRNA for G-pr...    50   1e-04
AF216967_1(AF216967|pid:none) Homo sapiens putative vascular ind...    48   4e-04
(Q8K3V3) RecName: Full=G-protein coupled receptor 56; Flags: Pre...    47   0.001
AK295345_1(AK295345|pid:none) Homo sapiens cDNA FLJ55998 complet...    46   0.002
AF166382_1(AF166382|pid:none) Mus musculus serpentine receptor (...    45   0.003
AY181246_1(AY181246|pid:none) Homo sapiens G protein-coupled rec...    45   0.004
(Q96K78) RecName: Full=Probable G-protein coupled receptor 128; ...    45   0.004
protein update 2009. 3.31
PSORT 

 psg: 0.75  gvh: 0.42  alm: 0.22  top: 1.17  tms: 0.07  mit: 0.23  mip: 0.00
 nuc: 0.10  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 1.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: cytoplasmic
	 20.0 %: nuclear
	 12.0 %: mitochondrial
	 12.0 %: vesicles of secretory system
	  8.0 %: Golgi
	  8.0 %: endoplasmic reticulum
	  4.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal
	  4.0 %: plasma membrane

>> prediction for CHF221 is cyt
5' end seq. ID CHF221F
5' end seq. 
>CHF221F.Seq
AACTGCAACCTCTGGTAAAGAGACAAACTACGATAATGTTACAGTTATCATTTCAAAGAA
ACCAATCACTGCAGTAATTGATGGAGCCGATAAAGAATTCCCATTAAATACTTTAATAAC
TTTAGATGCTTCAAAATCTTTTGATCCAACAACTACTTCAACAGTTTNNNNNNNNNN
Length of 5' end seq. 177
3' end seq. ID CHF221Z
3' end seq. 
>CHF221Z.Seq
NNNNNNNNNNCTTCAGACATTTTAGTGGTATCCAATTCATTGGCAGATGGTATTCTTCAA
AGAACTGCAATTGGTGAAGAACCATTTACAGTTGCAAGTGATAAAGGTTTTTCGATTACA
ACATTTAAAACTCTACATATGGAATTAAGTAAGAGTGGTCAATCGATTGGTTTCAAAAAG
GATGATGAAGGTCAATCTCTTGATGGTGAAAAACTTGCTTTCACTCCAAAATTCAGTATT
CCATCAAGTGTTTTATCAAAGAATAAATTAAAACAAACCTCTGAAATCACTGGTAAACTA
ATTACAGTTCCATCAAATATTCATTTCAATCATAAATCTGATCAAACCATTTTCCCAAAA
ACTGTTTCACTTGAACTTTTCAATGGTGGCTCTCAATTATCAATCAATGGCCTTAAAGAT
CAAATTATCATTGATATGGGAAAACATTCACCACCAAAAGATAAAGAATTTAAATGTGGT
TGGTGGAGCTCCAATAAAAAAGAATGGTTAACAGATGGTTGTTCAACCCATAAAGTAGAT
GGTAATATTCAATGTAAATGTAACCATCTTACAGAGTTTGGTTTAATTGAATTTGCAAAA
TCCTCTCAAAACATTTGGATCATTATTGGACCAGTAGTTGGTGGTGTTGTATTTGCAGCT
GCAATGGTTGGTACTGTTGTATTGGTAAAGAGAAAGAAAAACAAAAACAAAAAGAACAAA
TCAAAGAAAACCAAAGAACAAATCAACAAGNAAAAAGNAAGNACAAAAAGAAACA
Length of 3' end seq. 775
Connected seq. ID CHF221P
Connected seq. 
>CHF221P.Seq
AACTGCAACCTCTGGTAAAGAGACAAACTACGATAATGTTACAGTTATCATTTCAAAGAA
ACCAATCACTGCAGTAATTGATGGAGCCGATAAAGAATTCCCATTAAATACTTTAATAAC
TTTAGATGCTTCAAAATCTTTTGATCCAACAACTACTTCAACAGTTT----------CTT
CAGACATTTTAGTGGTATCCAATTCATTGGCAGATGGTATTCTTCAAAGAACTGCAATTG
GTGAAGAACCATTTACAGTTGCAAGTGATAAAGGTTTTTCGATTACAACATTTAAAACTC
TACATATGGAATTAAGTAAGAGTGGTCAATCGATTGGTTTCAAAAAGGATGATGAAGGTC
AATCTCTTGATGGTGAAAAACTTGCTTTCACTCCAAAATTCAGTATTCCATCAAGTGTTT
TATCAAAGAATAAATTAAAACAAACCTCTGAAATCACTGGTAAACTAATTACAGTTCCAT
CAAATATTCATTTCAATCATAAATCTGATCAAACCATTTTCCCAAAAACTGTTTCACTTG
AACTTTTCAATGGTGGCTCTCAATTATCAATCAATGGCCTTAAAGATCAAATTATCATTG
ATATGGGAAAACATTCACCACCAAAAGATAAAGAATTTAAATGTGGTTGGTGGAGCTCCA
ATAAAAAAGAATGGTTAACAGATGGTTGTTCAACCCATAAAGTAGATGGTAATATTCAAT
GTAAATGTAACCATCTTACAGAGTTTGGTTTAATTGAATTTGCAAAATCCTCTCAAAACA
TTTGGATCATTATTGGACCAGTAGTTGGTGGTGTTGTATTTGCAGCTGCAATGGTTGGTA
CTGTTGTATTGGTAAAGAGAAAGAAAAACAAAAACAAAAAGAACAAATCAAAGAAAACCA
AAGAACAAATCAACAAGNAAAAAGNAAGNACAAAAAGAAACA
Length of connected seq. 932
Full length Seq ID -
Full length Seq. -
Length of full length seq. -