>CHF211 (CHF211Q) /CSM/CH/CHF2-A/CHF211Q.Seq.d/
ATTTTATTTCATTTTCGAAAACTTTAAAACACACTTTCATATAATTTTTTTTCTTTTATA
TTAAAATTTGTTTAAATATAAAATGTTAAGATCATTAAAAGCTTTAAAACTTAACAGTTT
AAATGCCCAAAAAGGTATTCGTCAATTCTGTTCAGATTCTAAAATCTCTGGTCAAGTTAT
TGGTATTGATTTAGGTACAACCAATTCTTGTGTTGCTGTTATGCAAGGTGCTGAAGCCCG
TGTTTTAGAAAATGCTGAAGGTGGTCGTACCACTCCATCAGTTGTTGCCTTCACTGAAGA
CAACCAAAAAATCGTTGGTTTACCAGCTAAAAGACAAATGGTTACAAATGCTGAAAATAC
ATTATTTGCTACTAAACGTTTAATTGGTCGTAGATTTGATGATCCAATGACTAAAAAAGA
TATGACAATGGTACCATATAAGATTGTTAAAGGTCCAAATGGTGATGCATGGTTTGAAGT
TAAAGGTAAAATGATTTCACCATCAGAAGCAGGTGCTATGGTTTTACAAAAGATGAAAGA
AACCGCCGAAACCAATTTANGTGGCCCAGTTACAGATGCTGTTATTACCGTTCCAGCATA
CTTTGATGATAGTCAACGTCAAGCCACTCGTGATGCAGGTXXXXXXXXXXAATATTCCAG
CCTCAAAGACTGATACTTTCTCAACTGCTGCCGATGGTCAATCTGAAGTAGAAATTAAAG
TTTTCCAAGGTGAAAGAGAAATGGCCGTCGACAATAAACTCCTCGCCAAATTCACTTTAT
TCGGTCTCCCACCATTACCAAAAGGTGTCCCACAAATTGAAGTTACCTTTGATATTGATG
TCAATGGTATGTTACAAGTTATTGCTAAAGATAAAGCTACCGGTAAAGCTCAACAAGTTA
GAATTCAAACCAGTGGTGGTTTAAGCAAAGATGATATCGCTCGTATCCTTAAAGAATCTG
AAGCCAACGCTGAAGTTGATCGTAAACGTAAAGAATTAGTTGAAGCCAGAAACAATGGTG
AATCCGTTGTATACCAAGTTGAAAAAGATTTAGTTGAATTCAAAGATTACCTCTCTCAAC
CACAAACTGAAGAAATTAAAAAAGCAGTCCAAGCCGTTCGTGATGTCCTTGCCAAAGAAG
ATGGTTCAGCCATTGAAGCTGAAATTAAAAAATTACACGATTTAACTCGTTCATCTTTTG
AAACTGCCTACAAAGCTAAACTCGATTCATCTGCAAGCAAATCTTCATCAACTGAAAATA
AAGAAAACAAAGATAACACCACTGAAGCTGAATTCACTNAAAAGAAATAAATTTTTAAAT
TATTAAAAATAGTAAATAAAAAAAATAAAAAATTTAAAAAAAAATTTAAAAA

fyfifenfkthfhiifflly*NLFKYKMLRSLKALKLNSLNAQKGIRQFCSDSKISGQVI
GIDLGTTNSCVAVMQGAEARVLENAEGGRTTPSVVAFTEDNQKIVGLPAKRQMVTNAENT
LFATKRLIGRRFDDPMTKKDMTMVPYKIVKGPNGDAWFEVKGKMISPSEAGAMVLQKMKE
TAETNLXGPVTDAVITVPAYFDDSQRQATRDAG---

---NIPASKTDTFSTAADGQSEVEIKVFQGEREMAVDNKLLAKFTLFGLPPLPKGVPQIE
VTFDIDVNGMLQVIAKDKATGKAQQVRIQTSGGLSKDDIARILKESEANAEVDRKRKELV
EARNNGESVVYQVEKDLVEFKDYLSQPQTEEIKKAVQAVRDVLAKEDGSAIEAEIKKLHD
LTRSSFETAYKAKLDSSASKSSSTENKENKDNTTEAEFTXKK*ifkllkivnkknkkfkk
kfk

Frame A:
ilfhfrkl*ntlsynffsfilkfv*i*nvkiiksfkt*qfkcpkryssilfrf*nlwssy
wy*frynqflcccyarc*spcfrkc*rwsyhsiscclh*rqpknrwfts*ktngykc*ky
iicy*tfnws*i**snd*krydngti*dc*rskw*cmv*s*r*ndftirsrcygftkder
nrrnqfxwpsyrccyyrssil***stsshs*cr---

---NIPASKTDTFSTAADGQSEVEIKVFQGEREMAVDNKLLAKFTLFGLPPLPKGVPQIE
VTFDIDVNGMLQVIAKDKATGKAQQVRIQTSGGLSKDDIARILKESEANAEVDRKRKELV
EARNNGESVVYQVEKDLVEFKDYLSQPQTEEIKKAVQAVRDVLAKEDGSAIEAEIKKLHD
LTRSSFETAYKAKLDSSASKSSSTENKENKDNTTEAEFTXKK*ifkllkivnkknkkfkk
kfk

Frame B:
fyfifenfkthfhiifflly*NLFKYKMLRSLKALKLNSLNAQKGIRQFCSDSKISGQVI
GIDLGTTNSCVAVMQGAEARVLENAEGGRTTPSVVAFTEDNQKIVGLPAKRQMVTNAENT
LFATKRLIGRRFDDPMTKKDMTMVPYKIVKGPNGDAWFEVKGKMISPSEAGAMVLQKMKE
TAETNLXGPVTDAVITVPAYFDDSQRQATRDAG---

---ifqpqrlilsqllpmvnlk*klkfskvkekwpstinsspnslysvshhyqkvshklk
lplilmsmvcyklllkiklpvklnklefkpvvv*akmislvslknlkptlklivnvkn*l
kpetmvnplytklkki*lnskitslnhklkklkkqskpfvmslpkkmvqplklklknyti
*lvhllklptklnsihlqanlhqlkikktkitplklnslkrnkflny*k**ikkiknlkk
nlk

Frame C:
fisfsktlkhtfi*ffffyikiclnikc*dh*kl*nltv*mpkkvfvnsvqilkslvkll
vli*vqpilvlllckvlkpvf*kmlkvvvplhqllpslkttkkslvyqlkdkwlqmlkih
ylllnv*lvvdlmiq*lkki*qwyhirllkvqmvmhglklkvk*fhhqkqvlwfykr*kk
ppkpixvaqlqmlllpfqhtlmivnvkplvmq---

---ysslkd*yflnccrwsi*srn*sfpr*krngrrq*tprqihfirsptitkrcptn*s
yl*y*cqwyvtsyc*r*syr*ssts*nsnqwwfkqr*yrsyp*ri*sqr*s*s*t*ris*
sqkqw*ircips*krfs*iqrlplsttn*rn*ksspsrs*cpcqrrwfsh*s*n*kitrf
nsfif*nclqs*trfickqifin*k*rkqr*hh*s*ihxkeinf*iiknsk*kk*ki*kk
i*k

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHF211 (CHF211Q) /CSM/CH/CHF2-A/CHF211Q.Seq.d/                       2460   0.0
VHQ334 (VHQ334Q) /CSM/VH/VHQ3-B/VHQ334Q.Seq.d/                       1285   0.0
VHC763 (VHC763Q) /CSM/VH/VHC7-C/VHC763Q.Seq.d/                       1285   0.0
VHB739 (VHB739Q) /CSM/VH/VHB7-B/VHB739Q.Seq.d/                       1285   0.0
CHM184 (CHM184Q) /CSM/CH/CHM1-D/CHM184Q.Seq.d/                       1285   0.0
CHL664 (CHL664Q) /CSM/CH/CHL6-C/CHL664Q.Seq.d/                       1285   0.0
CHI137 (CHI137Q) /CSM/CH/CHI1-B/CHI137Q.Seq.d/                       1285   0.0
CHI781 (CHI781Q) /CSM/CH/CHI7-D/CHI781Q.Seq.d/                       1283   0.0
CFI557 (CFI557Q) /CSM/CF/CFI5-C/CFI557Q.Seq.d/                       1283   0.0
CHM559 (CHM559Q) /CSM/CH/CHM5-C/CHM559Q.Seq.d/                       1277   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AY170440|AY170440.1 Dictyostelium discoideum mitochondrial heat shock protein Hsp70 (hsp70) gene, complete cds; nuclear gene for mitochondrial product.  1285   0.0    3
AF539617|AF539617.1 Dictyostelium discoideum mitochondrial Hsp70 precursor (mhsp70) mRNA, complete cds; nuclear gene for mitochondrial product.  1285   0.0    1
AF419853|AF419853.1 Neocallimastix patriciarum heat shock protein 70 precursor (Hsp70) mRNA, complete cds.    76   1e-32  5
AW586153|AW586153.1 EST317776 MHAM Medicago truncatula/Glomus versiforme mixed EST library cDNA clone pMHAM-52M15, mRNA sequence.    74   4e-29  5
AW695785|AW695785.1 NF098G08ST1F1067 Developing stem Medicago truncatula cDNA clone NF098G08ST 5', mRNA sequence.    74   5e-29  5
BG646157|BG646157.1 EST507776 KV3 Medicago truncatula cDNA clone pKV3-48L11 5' end, mRNA sequence.    74   6e-29  5
AX536934|AX536934.1 Sequence 535 from Patent WO02064766.           68   6e-28  6
BI267815|BI267815.1 NF111G06IN1F1052 Insect herbivory Medicago truncatula cDNA clone NF111G06IN 5', mRNA sequence.    74   1e-25  4
BF650576|BF650576.1 NF090H10EC1F1090 Elicited cell culture Medicago truncatula cDNA clone NF090H10EC 5', mRNA sequence.    74   1e-25  4
CG936433|CG936433.1 MBEDF75TFC mth2 Medicago truncatula genomic clone 31M6, genomic survey sequence.    74   3e-25  4

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q8I0H7) RecName: Full=Heat shock 70 kDa protein, mitochondrial;...   367   e-100
DQ524993_1(DQ524993|pid:none) Sparus aurata glucose regulated pr...   235   3e-60
BC044175_1(BC044175|pid:none) Danio rerio heat shock protein 9, ...   233   9e-60
BC083504_1(BC083504|pid:none) Danio rerio heat shock protein 9, ...   233   9e-60
BT059314_1(BT059314|pid:none) Salmo salar clone ssal-rgf-540-298...   230   7e-59
AY326398_1(AY326398|pid:none) Mya arenaria mitochondrial mortali...   230   7e-59
CP001016_127(CP001016|pid:none) Beijerinckia indica subsp. indic...   229   1e-58
AM910991_319(AM910991|pid:none) Plasmodium knowlesi strain H chr...   229   1e-58
AB306281_1(AB306281|pid:none) Alligator mississippiensis HSP70C ...   229   1e-58
EF028390_1(EF028390|pid:none) Lytechinus variegatus mortalin mRN...   228   3e-58

 psg: 0.63  gvh: 0.50  alm: 0.44  top: 0.53  tms: 0.00  mit: 0.39  mip: 0.05
 nuc: 0.04  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 32.0 %: mitochondrial
	 28.0 %: cytoplasmic
	 28.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: plasma membrane
	  4.0 %: peroxisomal

>> prediction for CHF211 is mit

>CHF211F.Seq
ATTTTATTTCATTTTCGAAAACTTTAAAACACACTTTCATATAATTTTTTTTCTTTTATA
TTAAAATTTGTTTAAATATAAAATGTTAAGATCATTAAAAGCTTTAAAACTTAACAGTTT
AAATGCCCAAAAAGGTATTCGTCAATTCTGTTCAGATTCTAAAATCTCTGGTCAAGTTAT
TGGTATTGATTTAGGTACAACCAATTCTTGTGTTGCTGTTATGCAAGGTGCTGAAGCCCG
TGTTTTAGAAAATGCTGAAGGTGGTCGTACCACTCCATCAGTTGTTGCCTTCACTGAAGA
CAACCAAAAAATCGTTGGTTTACCAGCTAAAAGACAAATGGTTACAAATGCTGAAAATAC
ATTATTTGCTACTAAACGTTTAATTGGTCGTAGATTTGATGATCCAATGACTAAAAAAGA
TATGACAATGGTACCATATAAGATTGTTAAAGGTCCAAATGGTGATGCATGGTTTGAAGT
TAAAGGTAAAATGATTTCACCATCAGAAGCAGGTGCTATGGTTTTACAAAAGATGAAAGA
AACCGCCGAAACCAATTTANGTGGCCCAGTTACAGATGCTGTTATTACCGTTCCAGCATA
CTTTGATGATAGTCAACGTCAAGCCACTCGTGATGCAGGTNNNNNNNNNN

>CHF211Z.Seq
NNNNNNNNNNAATATTCCAGCCTCAAAGACTGATACTTTCTCAACTGCTGCCGATGGTCA
ATCTGAAGTAGAAATTAAAGTTTTCCAAGGTGAAAGAGAAATGGCCGTCGACAATAAACT
CCTCGCCAAATTCACTTTATTCGGTCTCCCACCATTACCAAAAGGTGTCCCACAAATTGA
AGTTACCTTTGATATTGATGTCAATGGTATGTTACAAGTTATTGCTAAAGATAAAGCTAC
CGGTAAAGCTCAACAAGTTAGAATTCAAACCAGTGGTGGTTTAAGCAAAGATGATATCGC
TCGTATCCTTAAAGAATCTGAAGCCAACGCTGAAGTTGATCGTAAACGTAAAGAATTAGT
TGAAGCCAGAAACAATGGTGAATCCGTTGTATACCAAGTTGAAAAAGATTTAGTTGAATT
CAAAGATTACCTCTCTCAACCACAAACTGAAGAAATTAAAAAAGCAGTCCAAGCCGTTCG
TGATGTCCTTGCCAAAGAAGATGGTTCAGCCATTGAAGCTGAAATTAAAAAATTACACGA
TTTAACTCGTTCATCTTTTGAAACTGCCTACAAAGCTAAACTCGATTCATCTGCAAGCAA
ATCTTCATCAACTGAAAATAAAGAAAACAAAGATAACACCACTGAAGCTGAATTCACTNA
AAAGAAATAAATTTTTAAATTATTAAAAATAGTAAATAAAAAAAATAAAAAATTTAAAAA
AAAATTTAAAAA

>CHF211P.Seq
ATTTTATTTCATTTTCGAAAACTTTAAAACACACTTTCATATAATTTTTTTTCTTTTATA
TTAAAATTTGTTTAAATATAAAATGTTAAGATCATTAAAAGCTTTAAAACTTAACAGTTT
AAATGCCCAAAAAGGTATTCGTCAATTCTGTTCAGATTCTAAAATCTCTGGTCAAGTTAT
TGGTATTGATTTAGGTACAACCAATTCTTGTGTTGCTGTTATGCAAGGTGCTGAAGCCCG
TGTTTTAGAAAATGCTGAAGGTGGTCGTACCACTCCATCAGTTGTTGCCTTCACTGAAGA
CAACCAAAAAATCGTTGGTTTACCAGCTAAAAGACAAATGGTTACAAATGCTGAAAATAC
ATTATTTGCTACTAAACGTTTAATTGGTCGTAGATTTGATGATCCAATGACTAAAAAAGA
TATGACAATGGTACCATATAAGATTGTTAAAGGTCCAAATGGTGATGCATGGTTTGAAGT
TAAAGGTAAAATGATTTCACCATCAGAAGCAGGTGCTATGGTTTTACAAAAGATGAAAGA
AACCGCCGAAACCAATTTANGTGGCCCAGTTACAGATGCTGTTATTACCGTTCCAGCATA
CTTTGATGATAGTCAACGTCAAGCCACTCGTGATGCAGGT----------AATATTCCAG
CCTCAAAGACTGATACTTTCTCAACTGCTGCCGATGGTCAATCTGAAGTAGAAATTAAAG
TTTTCCAAGGTGAAAGAGAAATGGCCGTCGACAATAAACTCCTCGCCAAATTCACTTTAT
TCGGTCTCCCACCATTACCAAAAGGTGTCCCACAAATTGAAGTTACCTTTGATATTGATG
TCAATGGTATGTTACAAGTTATTGCTAAAGATAAAGCTACCGGTAAAGCTCAACAAGTTA
GAATTCAAACCAGTGGTGGTTTAAGCAAAGATGATATCGCTCGTATCCTTAAAGAATCTG
AAGCCAACGCTGAAGTTGATCGTAAACGTAAAGAATTAGTTGAAGCCAGAAACAATGGTG
AATCCGTTGTATACCAAGTTGAAAAAGATTTAGTTGAATTCAAAGATTACCTCTCTCAAC
CACAAACTGAAGAAATTAAAAAAGCAGTCCAAGCCGTTCGTGATGTCCTTGCCAAAGAAG
ATGGTTCAGCCATTGAAGCTGAAATTAAAAAATTACACGATTTAACTCGTTCATCTTTTG
AAACTGCCTACAAAGCTAAACTCGATTCATCTGCAAGCAAATCTTCATCAACTGAAAATA
AAGAAAACAAAGATAACACCACTGAAGCTGAATTCACTNAAAAGAAATAAATTTTTAAAT
TATTAAAAATAGTAAATAAAAAAAATAAAAAATTTAAAAAAAAATTTAAAAA