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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CHF204
Library CH
(Link to library)
Clone ID CHF204
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16361-1
Original site URL
Representative seq. ID CHF204P
(Link to Original site)
Representative DNA sequence 
>CHF204 (CHF204Q) /CSM/CH/CHF2-A/CHF204Q.Seq.d/
TTAAAGAAAAAGAATACTGTGATGGACTTTGGTGAAGATGATGGTGGTAGTGGTGACGAT
GGTAATATAACCGAAGGTTTACCAATTAGTGAAGGTATGGATGATTTACCATCATCTTCA
AACTCTCGTGGCGGCAGCGGTAATGATGAACAAAAGAAACGGTTCCCAAAGGAAATGAAT
TCTCCATCATCGGAATATGGCACAACAAGTGGCGGTCAAAGATTTGATACATTGGTTGAT
CCTGATATTAGTTTAGCAGAGATGGAAGAGAAAATGCGTCAACACAAGGTATACCAAGAG
CAACAACAGCAACAACAACAACAGCAACAACAACAAAAGCAAAAAGATAAAGAGTTGTCA
TCACAAAAGAAGAAACCATCATCAATGCAATTAAGTAAAAAGAAACATGTCGCCAAAGAA
GATTCTGAAACATTAGAGACAATCATTGGTGAAGAGAAGAAGGAGGTTGTATTTGAAGTT
AAACCATACTTTTCTCATGCAATTCTTCAAGCAACCATGGCGGTATTTTTAATTTGGAAT
ATTTTTTACTTTGCATATCGTGCCGGTTGGACAATGAATAGAACCGATTATATCACATTC
TCTTATTCXXXXXXXXXXTTTCATTCTCGTCGGTATGATTCCTGTAATGGTGTTACCAAG
AGTCATTCAATATATGATTCTTAGAGCCAAGAGACCTTATGAAGCCGGTAAGAGTGGTCC
ATCACTTTGGGTAGAAGCTACTGATCTTTGGAGAGCTGAACAAACTTTCTTTGGTTTTGC
TGGTACTTATATTTCATCATGGAGAGAAGGTTCCGCTTCAATCGTTAAACTTCTTAAAGC
TAGAAAGATTAGTCGTCATAAGTTAGCCATGTGGAATTGGAAGAGAGATTTCGTAAAGAA
ACCAGTAGTTTGTGAAGTTTTCCGTCAAACTAAATTGGTCAATGAAAATGATAATGCTCA
AGAATCAAGTGGTAAACATAAGGCTGAACAATCTTTCCGTACTTCAAACAAAGAATCTGA
TACCATTAAAAATTCAAGACTTTTCCTTCCAAATATTATCCTCTTTGTTGTTAATATCTT
GGCTATGATGAGTGCTGTACTTAGATTTAATTGTTTCCAAAATGATATGTGGCTTTTGGT
TGTCGTAGCTGGTTTCTCTTTCTCTACCTTATGGCATTTATGGTCTTTCATTCCAATGGC
TCTTCGTCAAAGTGAAAAACAATGGCCATATGCTTCTTCATACCATGCTCACAATATTGT
TTTATTCTGGTACTGGTTTCTGGTATACTCTTGTGAGTCAAAGTT
sequence update 2002.10.25
Translated Amino Acid sequence 
LKKKNTVMDFGEDDGGSGDDGNITEGLPISEGMDDLPSSSNSRGGSGNDEQKKRFPKEMN
SPSSEYGTTSGGQRFDTLVDPDISLAEMEEKMRQHKVYQEQQQQQQQQQQQQKQKDKELS
SQKKKPSSMQLSKKKHVAKEDSETLETIIGEEKKEVVFEVKPYFSHAILQATMAVFLIWN
IFYFAYRAGWTMNRTDYITFSY---

---FILVGMIPVMVLPRVIQYMILRAKRPYEAGKSGPSLWVEATDLWRAEQTFFGFAGTY
ISSWREGSASIVKLLKARKISRHKLAMWNWKRDFVKKPVVCEVFRQTKLVNENDNAQESS
GKHKAEQSFRTSNKESDTIKNSRLFLPNIILFVVNILAMMSAVLRFNCFQNDMWLLVVVA
GFSFSTLWHLWSFIPMALRQSEKQWPYASSYHAHNIVLFWYWFLVYSCESK
Translated Amino Acid sequence (All Frames) 
Frame A:
LKKKNTVMDFGEDDGGSGDDGNITEGLPISEGMDDLPSSSNSRGGSGNDEQKKRFPKEMN
SPSSEYGTTSGGQRFDTLVDPDISLAEMEEKMRQHKVYQEQQQQQQQQQQQQKQKDKELS
SQKKKPSSMQLSKKKHVAKEDSETLETIIGEEKKEVVFEVKPYFSHAILQATMAVFLIWN
IFYFAYRAGWTMNRTDYITFSY---

---fhsrrydscngvtkshsiyds*sqetl*sr*ewsitlgrsy*sles*tnflwfcwyl
yfimerrfrfnr*ts*s*kd*ss*vshveleerfrketssl*sfpsn*igq*k**csrik
w*t*g*tifpyfkqri*yh*kfktfpskyyplcc*ylgydecct*i*lfpk*yvafgcrs
wflflylmafmvfhsngsssk*ktmaicffipcsqycfilvlvsgill*vkv

Frame B:
*rkril*wtlvkmmvvvvtmvi*pkvyqlvkvwmiyhhlqtlvaaavmmnkrngsqrk*i
lhhrnmaqqvavkdlihwlililv*qrwkrkcvntrytksnnsnnnnsnnnkskkiksch
hkrrnhhqcn*vkrnmspkkilkh*rqslvkrrrrlylklnhtflmqffkqpwryf*fgi
fftlhivpvgq*iepiishsli---

---FILVGMIPVMVLPRVIQYMILRAKRPYEAGKSGPSLWVEATDLWRAEQTFFGFAGTY
ISSWREGSASIVKLLKARKISRHKLAMWNWKRDFVKKPVVCEVFRQTKLVNENDNAQESS
GKHKAEQSFRTSNKESDTIKNSRLFLPNIILFVVNILAMMSAVLRFNCFQNDMWLLVVVA
GFSFSTLWHLWSFIPMALRQSEKQWPYASSYHAHNIVLFWYWFLVYSCESK

Frame C:
kekeycdglw*r*ww*w*rw*ynrrftn**ryg*ftiifklswrqr***tketvpkgnef
siigiwhnkwrski*yig*s*y*fsrdgrenastqgiprattattttatttkakr*rvvi
tkeetiinaik*ketcrqrrf*nirdnhw*reeggci*s*tilfscnsssnhggifnley
fllciscrldne*nrlyhillf---

---sfssv*fl*wcyqesfni*fleprdlmkpvrvvhhfg*kllifgelnklslvllvli
fhhgekvplqslnflklerlvvis*pcgigreis*rnq*fvkfsvklnwsmkmimlknqv
vnirlnnlsvlqtknliplkiqdfsfqilssllliswl**vlyldlivskmicgfwls*l
vslslpygiyglsfqwlfvkvknnghmllhtmltilfysgtgfwytlvsqs
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHF204 (CHF204Q) /CSM/CH/CHF2-A/CHF204Q.Seq.d/                       1861   0.0
CHL393 (CHL393Q) /CSM/CH/CHL3-D/CHL393Q.Seq.d/                       1350   0.0
CHQ720 (CHQ720Q) /CSM/CH/CHQ7-A/CHQ720Q.Seq.d/                       1342   0.0
SHG409 (SHG409Q) /CSM/SH/SHG4-A/SHG409Q.Seq.d/                       1340   0.0
CHE730 (CHE730Q) /CSM/CH/CHE7-B/CHE730Q.Seq.d/                       1336   0.0
SHA559 (SHA559Q) /CSM/SH/SHA5-C/SHA559Q.Seq.d/                       1334   0.0
SHG445 (SHG445Q) /CSM/SH/SHG4-B/SHG445Q.Seq.d/                       1332   0.0
SHG416 (SHG416Q) /CSM/SH/SHG4-A/SHG416Q.Seq.d/                       1332   0.0
SHG355 (SHG355Q) /CSM/SH/SHG3-C/SHG355Q.Seq.d/                       1332   0.0
AHH550 (AHH550Q) /CSM/AH/AHH5-C/AHH550Q.Seq.d/                       1332   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AF163835|AF163835.1 Dictyostelium discoideum cellulose synthase (dcsA) mRNA, complete cds.  1289   0.0    2
AC006097|AC006097.1 Homo sapiens chromosome 10 clone CIT987SK-1031G15 map 10q25, *** SEQUENCING IN PROGRESS ***, 1 ordered pieces.    54   0.006  1
AC004480|AC004480.7 Homo sapiens chromosome 4, WORKING DRAFT SEQUENCE, 7 unordered pieces.    54   0.006  1
AL390197|AL390197.19 Human DNA sequence from clone RP11-190F19 on chromosome 10.    54   0.006  1
AC093323|AC093323.3 Homo sapiens BAC clone RP11-539L10 from 4, complete sequence.    54   0.006  1
AC132615|AC132615.3 Mus musculus chromosome 9 clone RP23-301J15, WORKING DRAFT SEQUENCE, 5 unordered pieces.    36   0.017  8
AC096106|AC096106.5 Rattus norvegicus clone CH230-27L19, *** SEQUENCING IN PROGRESS ***, 2 unordered pieces.    44   0.069  4
AC127134|AC127134.3 Rattus norvegicus clone CH230-108E5, *** SEQUENCING IN PROGRESS ***, 1 ordered piece.    36   0.22   9
AC094533|AC094533.5 Rattus norvegicus clone CH230-4L19, *** SEQUENCING IN PROGRESS ***, 3 unordered pieces.    36   0.26   9
AC122089|AC122089.4 Rattus norvegicus clone CH230-270L9, *** SEQUENCING IN PROGRESS ***, 3 unordered pieces.    48   0.37   1
dna update 2004. 9.27
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q9U720) RecName: Full=Cellulose synthase catalytic subunit A [U...   441   e-122
CP001279_992(CP001279|pid:none) Nautilia profundicola AmH, compl...    40   0.17
CP001048_907(CP001048|pid:none) Yersinia pseudotuberculosis PB1/...    35   4.3
CP001025_1222(CP001025|pid:none) Burkholderia ambifaria MC40-6 c...    35   4.3
EF678041_1(EF678041|pid:none) Picea sitchensis clone WS02821_C11...    34   9.6
protein update 2009. 3.31
PSORT 

 psg: 0.55  gvh: 0.40  alm: 0.24  top: 0.57  tms: 0.27  mit: 0.16  mip: 0.00
 nuc: 0.06  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 1.00  m_ : 0.00 

	 36.0 %: plasma membrane
	 16.0 %: vacuolar
	 16.0 %: nuclear
	 16.0 %: endoplasmic reticulum
	  8.0 %: mitochondrial
	  4.0 %: Golgi
	  4.0 %: cytoplasmic

>> prediction for CHF204 is pla
5' end seq. ID CHF204F
5' end seq. 
>CHF204F.Seq
TTAAAGAAAAAGAATACTGTGATGGACTTTGGTGAAGATGATGGTGGTAGTGGTGACGAT
GGTAATATAACCGAAGGTTTACCAATTAGTGAAGGTATGGATGATTTACCATCATCTTCA
AACTCTCGTGGCGGCAGCGGTAATGATGAACAAAAGAAACGGTTCCCAAAGGAAATGAAT
TCTCCATCATCGGAATATGGCACAACAAGTGGCGGTCAAAGATTTGATACATTGGTTGAT
CCTGATATTAGTTTAGCAGAGATGGAAGAGAAAATGCGTCAACACAAGGTATACCAAGAG
CAACAACAGCAACAACAACAACAGCAACAACAACAAAAGCAAAAAGATAAAGAGTTGTCA
TCACAAAAGAAGAAACCATCATCAATGCAATTAAGTAAAAAGAAACATGTCGCCAAAGAA
GATTCTGAAACATTAGAGACAATCATTGGTGAAGAGAAGAAGGAGGTTGTATTTGAAGTT
AAACCATACTTTTCTCATGCAATTCTTCAAGCAACCATGGCGGTATTTTTAATTTGGAAT
ATTTTTTACTTTGCATATCGTGCCGGTTGGACAATGAATAGAACCGATTATATCACATTC
TCTTATTCNNNNNNNNNN
Length of 5' end seq. 618
3' end seq. ID CHF204Z
3' end seq. 
>CHF204Z.Seq
NNNNNNNNNNTTTCATTCTCGTCGGTATGATTCCTGTAATGGTGTTACCAAGAGTCATTC
AATATATGATTCTTAGAGCCAAGAGACCTTATGAAGCCGGTAAGAGTGGTCCATCACTTT
GGGTAGAAGCTACTGATCTTTGGAGAGCTGAACAAACTTTCTTTGGTTTTGCTGGTACTT
ATATTTCATCATGGAGAGAAGGTTCCGCTTCAATCGTTAAACTTCTTAAAGCTAGAAAGA
TTAGTCGTCATAAGTTAGCCATGTGGAATTGGAAGAGAGATTTCGTAAAGAAACCAGTAG
TTTGTGAAGTTTTCCGTCAAACTAAATTGGTCAATGAAAATGATAATGCTCAAGAATCAA
GTGGTAAACATAAGGCTGAACAATCTTTCCGTACTTCAAACAAAGAATCTGATACCATTA
AAAATTCAAGACTTTTCCTTCCAAATATTATCCTCTTTGTTGTTAATATCTTGGCTATGA
TGAGTGCTGTACTTAGATTTAATTGTTTCCAAAATGATATGTGGCTTTTGGTTGTCGTAG
CTGGTTTCTCTTTCTCTACCTTATGGCATTTATGGTCTTTCATTCCAATGGCTCTTCGTC
AAAGTGAAAAACAATGGCCATATGCTTCTTCATACCATGCTCACAATATTGTTTTATTCT
GGTACTGGTTTCTGGTATACTCTTGTGAGTCAAAGTT
Length of 3' end seq. 697
Connected seq. ID CHF204P
Connected seq. 
>CHF204P.Seq
TTAAAGAAAAAGAATACTGTGATGGACTTTGGTGAAGATGATGGTGGTAGTGGTGACGAT
GGTAATATAACCGAAGGTTTACCAATTAGTGAAGGTATGGATGATTTACCATCATCTTCA
AACTCTCGTGGCGGCAGCGGTAATGATGAACAAAAGAAACGGTTCCCAAAGGAAATGAAT
TCTCCATCATCGGAATATGGCACAACAAGTGGCGGTCAAAGATTTGATACATTGGTTGAT
CCTGATATTAGTTTAGCAGAGATGGAAGAGAAAATGCGTCAACACAAGGTATACCAAGAG
CAACAACAGCAACAACAACAACAGCAACAACAACAAAAGCAAAAAGATAAAGAGTTGTCA
TCACAAAAGAAGAAACCATCATCAATGCAATTAAGTAAAAAGAAACATGTCGCCAAAGAA
GATTCTGAAACATTAGAGACAATCATTGGTGAAGAGAAGAAGGAGGTTGTATTTGAAGTT
AAACCATACTTTTCTCATGCAATTCTTCAAGCAACCATGGCGGTATTTTTAATTTGGAAT
ATTTTTTACTTTGCATATCGTGCCGGTTGGACAATGAATAGAACCGATTATATCACATTC
TCTTATTC----------TTTCATTCTCGTCGGTATGATTCCTGTAATGGTGTTACCAAG
AGTCATTCAATATATGATTCTTAGAGCCAAGAGACCTTATGAAGCCGGTAAGAGTGGTCC
ATCACTTTGGGTAGAAGCTACTGATCTTTGGAGAGCTGAACAAACTTTCTTTGGTTTTGC
TGGTACTTATATTTCATCATGGAGAGAAGGTTCCGCTTCAATCGTTAAACTTCTTAAAGC
TAGAAAGATTAGTCGTCATAAGTTAGCCATGTGGAATTGGAAGAGAGATTTCGTAAAGAA
ACCAGTAGTTTGTGAAGTTTTCCGTCAAACTAAATTGGTCAATGAAAATGATAATGCTCA
AGAATCAAGTGGTAAACATAAGGCTGAACAATCTTTCCGTACTTCAAACAAAGAATCTGA
TACCATTAAAAATTCAAGACTTTTCCTTCCAAATATTATCCTCTTTGTTGTTAATATCTT
GGCTATGATGAGTGCTGTACTTAGATTTAATTGTTTCCAAAATGATATGTGGCTTTTGGT
TGTCGTAGCTGGTTTCTCTTTCTCTACCTTATGGCATTTATGGTCTTTCATTCCAATGGC
TCTTCGTCAAAGTGAAAAACAATGGCCATATGCTTCTTCATACCATGCTCACAATATTGT
TTTATTCTGGTACTGGTTTCTGGTATACTCTTGTGAGTCAAAGTT
Length of connected seq. 1295
Full length Seq ID -
Full length Seq. -
Length of full length seq. -