>CHF171 (CHF171Q) /CSM/CH/CHF1-C/CHF171Q.Seq.d/
CAAATATAAATGGTCAATTGTTCCAGAGATTCAATTGGCAAGTTCAAAAGATTATACAGG
TCCAACTTTAAATATTCCTTCAAGTAAATGGGTTTATGATACAACTTATGTTATTACATT
AACTGCAACCTCTGGTAAAGAGACAAACTACGATAATGTTACAGTTATCATTTCAAAGAA
ACCAATCACTGCAGTAATTGATGGAGCCGATAAAGAATTCCCATTAAATACTTTAATAAC
TTTAGATGCTTCAAAATCTTTTGATCCAACAACTACTTCAACAGTTTCCAAAGAACTTGA
TGATTCAACCTCTACACAACAACAATCATTTACTTATGCATGGAGATGTATCAATAAAAG
TGGCAAAGATTGCGGATTAAATTTAGAATCTGAACCAATTATTACATTTAACTCTTCAAG
ATTGAATGTTGGTGTTTTCCATTTCTCTGTTGATGTTACCCCAAATGGTGATGCCACAAG
AAAGTGGTTCTGCTAGTGCCACTGTTACCATTGTAAATACTCCAGTAATTGATGTTATTA
TCTCACCATTACCAACAACCATTGATCCAACTCAAAAATTGGTACTCTATACAGTAGTTA
GTGGTAATGATGCATCTTCATCAAATTTAACCTATGAATGGAATATGCTXXXXXXXXXXG
AACTGCAATTGGTGAAGAACCATTTACAGTTGCAAGTGATAAAGGTTTCTCGATTACAAC
ATTTAAAACTCTACATATGGAATTAAGTAAGAGTGGTCAATCGATTGGTTTCAAAAAGGA
TGATGAAGGTCAATCTCTTGATGGTGAAAAACTTGCTTTCACTCCAAAATTCAGTATTCC
ATCAAGTGTTTTATCAAAGAATAAATTAAAACAAACCTCTGAAATCACTGGTAAACTAAT
TACAGTTCCATCAAATATTCATTTCAATCATAAATCTGATCAAACCATTTTCCCAAAAAC
TGTTTCACTTGAACTTTTCAATGGTGGCTCTCAATTATCAATCAATGGCCTTAAAGATCA
AATTATCATTGATATGGGAAAACATTCACCACCAAAAGATAAAGAATTTAAATGTGGTTG
GTGGAGCTCCAATAAAAAAGAATGGTTAACAGATGGTTGTTCAACCCATAAAGTAGATGG
TAATATTCAATGTAAATGTAACCATCTTACAGAGTTTGGTTTAATTGAATTTGCAAAATC
CTCTCAAAACATTTGGATCATTATTGGACCAGTAGTTGGTGGTGTTGTATTTGCAGCTGC
AATGGTTGGTACTGTTGTATTGGTAAAGAGAAAGAAAAACAAAAACAAAAAGAACAAATC
AAAGAAAACCAAAGAACAAATCAACAAAGAAAAAGAAGACAAAAAGAAACAAGCAAAAGA
AAAGAAAGAAG

ningqlfqrfnwqvqkiiqvql*iflqvngfmiqlmllh*lqplvkrqttimlqlsfqrn
qslq*lmepiknsh*il**l*mlqnlliqqllqqfpknlmiqplhnnnhllmhgdvsikv
akiad*i*nlnqllhltlqd*MLVFSISLLMLPQMVMPQESGSASATVTIVNTPVIDVII
SPLPTTIDPTQKLVLYTVVSGNDASSSNLTYEWNM---

---TAIGEEPFTVASDKGFSITTFKTLHMELSKSGQSIGFKKDDEGQSLDGEKLAFTPKF
SIPSSVLSKNKLKQTSEITGKLITVPSNIHFNHKSDQTIFPKTVSLELFNGGSQLSINGL
KDQIIIDMGKHSPPKDKEFKCGWWSSNKKEWLTDGCSTHKVDGNIQCKCNHLTEFGLIEF
AKSSQNIWIIIGPVVGGVVFAAAMVGTVVLVKRKKNKNKKNKSKKTKEQINKEKEDKKKQ
AKEKKE

Frame A:
qi*mvncsrdsigkfkrlyrsnfkysfk*mgl*ynlcyyincnlw*rdklr*cysyhfke
tnhcsn*wsr*ripikyfnnfrcfkif*snnyfnsfqrt**fnlytttiiylcmemyq*k
wqrlrikfri*tnyyi*lfkiecwcfpflc*cypkw*chkkvvllvpllpl*ilq*lmll
shhyqqpliqlknwysiq*lvvmmhlhqi*pmngic---

---elqlvknhlqlqvikvsrlqhlklyiwn*vrvvnrlvskrmmkvnllmvknllslqn
svfhqvfyqrin*nkplkslvn*lqfhqifisiinlikpfsqklfhlnfsmvalnyqsma
lkiklsliwenihhqkiknlnvvggapikkng*qmvvqpik*mvifnvnvtilqslv*ln
lqnplktfgslldq*lvvlylqlqwlvllyw*rerktktkrtnqrkpknkstkkkktkrn
kqkkrkk

Frame B:
kykwsivpeiqlasskdytgptlnipsskwvydttyvitltatsgketnydnvtviiskk
pitavidgadkefplntlitldasksfdptttstvskelddststqqqsftyawrcinks
gkdcglnlesepiitfnssrlnvgvfhfsvdvtpngdatrkwfc*chcyhckyssn*cyy
ltitnnh*snskigtlyss*w**cifikfnl*meya---

---ncnw*rtiysck**rfldyni*nstygik*ewsidwfqkg**rsis*w*ktcfhski
qysikcfike*iktnl*nhw*tnyssikysfqs*i*snhfpkncft*tfqwwlsiinqwp
*rsnyh*ygktfttkr*ri*mwlvelq*krmvnrwlfnp*srw*ysm*m*psyrvwfn*i
ckilskhldhywtsswwccicscngwyccigkekekqkqkeqikenqrtnqqrkrrqket
skrker

Frame C:
ningqlfqrfnwqvqkiiqvql*iflqvngfmiqlmllh*lqplvkrqttimlqlsfqrn
qslq*lmepiknsh*il**l*mlqnlliqqllqqfpknlmiqplhnnnhllmhgdvsikv
akiad*i*nlnqllhltlqd*MLVFSISLLMLPQMVMPQESGSASATVTIVNTPVIDVII
SPLPTTIDPTQKLVLYTVVSGNDASSSNLTYEWNM---

---TAIGEEPFTVASDKGFSITTFKTLHMELSKSGQSIGFKKDDEGQSLDGEKLAFTPKF
SIPSSVLSKNKLKQTSEITGKLITVPSNIHFNHKSDQTIFPKTVSLELFNGGSQLSINGL
KDQIIIDMGKHSPPKDKEFKCGWWSSNKKEWLTDGCSTHKVDGNIQCKCNHLTEFGLIEF
AKSSQNIWIIIGPVVGGVVFAAAMVGTVVLVKRKKNKNKKNKSKKTKEQINKEKEDKKKQ
AKEKKE

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHF171 (CHF171Q) /CSM/CH/CHF1-C/CHF171Q.Seq.d/                       2486   0.0
SHF578 (SHF578Q) /CSM/SH/SHF5-D/SHF578Q.Seq.d/                       1259   0.0
SHL562 (SHL562Q) /CSM/SH/SHL5-C/SHL562Q.Seq.d/                       1239   0.0
SHL489 (SHL489Q) /CSM/SH/SHL4-D/SHL489Q.Seq.d/                       1239   0.0
SHL119 (SHL119Q) /CSM/SH/SHL1-A/SHL119Q.Seq.d/                       1239   0.0
SHK878 (SHK878Q) /CSM/SH/SHK8-D/SHK878Q.Seq.d/                       1239   0.0
SHK593 (SHK593Q) /CSM/SH/SHK5-D/SHK593Q.Seq.d/                       1239   0.0
SHK142 (SHK142Q) /CSM/SH/SHK1-B/SHK142Q.Seq.d/                       1239   0.0
SHJ824 (SHJ824Q) /CSM/SH/SHJ8-A/SHJ824Q.Seq.d/                       1239   0.0
SHG125 (SHG125Q) /CSM/SH/SHG1-B/SHG125Q.Seq.d/                       1239   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AP003131|AP003131.2 Staphylococcus aureus subsp. aureus N315 genomic DNA, complete genome, section 3/10.    34   0.020  14
AP004171|AP004171.1 Mycoplasma penetrans HF-2 DNA, complete genome, section 2/5.    30   0.34   15
AC118721|AC118721.2 Mus musculus clone RP24-157I20, WORKING DRAFT SEQUENCE, 7 unordered pieces.    32   0.37   9
AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.    32   0.39   18
AC103541|AC103541.6 Homo sapiens BAC clone RP11-818M13 from 4, complete sequence.    48   0.39   1
AC097518|AC097518.2 Homo sapiens BAC clone RP11-487E13 from 4, complete sequence.    48   0.39   1
AC024453|AC024453.2 Homo sapiens chromosome 4 clone RP11-2B17 map 4, WORKING DRAFT SEQUENCE, 51 unordered pieces.    48   0.39   1
Z70208|Z70208.1 Caenorhabditis elegans cosmid F54B11.              48   0.39   1
CK579682|CK579682.1 IST_WI5_25671 AD-wrmcDNA library Caenorhabditis elegans cDNA 5' similar to F54B11.3, mRNA sequence.    48   0.39   1
CK580072|CK580072.1 IST_WI5_26979 AD-wrmcDNA library Caenorhabditis elegans cDNA 5' similar to F54B11.3, mRNA sequence.    48   0.39   1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AK154648_1(AK154648|pid:none) Mus musculus NOD-derived CD11c +ve...    50   2e-04
(Q8R0T6) RecName: Full=Probable G-protein coupled receptor 97; F...    50   2e-04
AK037149_1(AK037149|pid:none) Mus musculus 6 days neonate skin c...    50   2e-04
AB041939_1(AB041939|pid:none) Homo sapiens KPG_006 mRNA for G-pr...    50   2e-04
AF216967_1(AF216967|pid:none) Homo sapiens putative vascular ind...    48   7e-04
(Q8K3V3) RecName: Full=G-protein coupled receptor 56; Flags: Pre...    47   0.002
AK295345_1(AK295345|pid:none) Homo sapiens cDNA FLJ55998 complet...    46   0.003
AF166382_1(AF166382|pid:none) Mus musculus serpentine receptor (...    45   0.005
AY181246_1(AY181246|pid:none) Homo sapiens G protein-coupled rec...    45   0.008
(Q96K78) RecName: Full=Probable G-protein coupled receptor 128; ...    45   0.008

 psg: 0.96  gvh: 0.68  alm: 0.22  top: 0.40  tms: 0.07  mit: 0.25  mip: 0.00
 nuc: 0.17  erl: 0.00  erm: 1.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 1.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 32.0 %: plasma membrane
	 20.0 %: extracellular, including cell wall
	 20.0 %: endoplasmic reticulum
	 12.0 %: mitochondrial
	 12.0 %: Golgi
	  4.0 %: nuclear

>> prediction for CHF171 is pla

>CHF171F.Seq
CAAATATAAATGGTCAATTGTTCCAGAGATTCAATTGGCAAGTTCAAAAGATTATACAGG
TCCAACTTTAAATATTCCTTCAAGTAAATGGGTTTATGATACAACTTATGTTATTACATT
AACTGCAACCTCTGGTAAAGAGACAAACTACGATAATGTTACAGTTATCATTTCAAAGAA
ACCAATCACTGCAGTAATTGATGGAGCCGATAAAGAATTCCCATTAAATACTTTAATAAC
TTTAGATGCTTCAAAATCTTTTGATCCAACAACTACTTCAACAGTTTCCAAAGAACTTGA
TGATTCAACCTCTACACAACAACAATCATTTACTTATGCATGGAGATGTATCAATAAAAG
TGGCAAAGATTGCGGATTAAATTTAGAATCTGAACCAATTATTACATTTAACTCTTCAAG
ATTGAATGTTGGTGTTTTCCATTTCTCTGTTGATGTTACCCCAAATGGTGATGCCACAAG
AAAGTGGTTCTGCTAGTGCCACTGTTACCATTGTAAATACTCCAGTAATTGATGTTATTA
TCTCACCATTACCAACAACCATTGATCCAACTCAAAAATTGGTACTCTATACAGTAGTTA
GTGGTAATGATGCATCTTCATCAAATTTAACCTATGAATGGAATATGCTNNNNNNNNNN

>CHF171Z.Seq
NNNNNNNNNNGAACTGCAATTGGTGAAGAACCATTTACAGTTGCAAGTGATAAAGGTTTC
TCGATTACAACATTTAAAACTCTACATATGGAATTAAGTAAGAGTGGTCAATCGATTGGT
TTCAAAAAGGATGATGAAGGTCAATCTCTTGATGGTGAAAAACTTGCTTTCACTCCAAAA
TTCAGTATTCCATCAAGTGTTTTATCAAAGAATAAATTAAAACAAACCTCTGAAATCACT
GGTAAACTAATTACAGTTCCATCAAATATTCATTTCAATCATAAATCTGATCAAACCATT
TTCCCAAAAACTGTTTCACTTGAACTTTTCAATGGTGGCTCTCAATTATCAATCAATGGC
CTTAAAGATCAAATTATCATTGATATGGGAAAACATTCACCACCAAAAGATAAAGAATTT
AAATGTGGTTGGTGGAGCTCCAATAAAAAAGAATGGTTAACAGATGGTTGTTCAACCCAT
AAAGTAGATGGTAATATTCAATGTAAATGTAACCATCTTACAGAGTTTGGTTTAATTGAA
TTTGCAAAATCCTCTCAAAACATTTGGATCATTATTGGACCAGTAGTTGGTGGTGTTGTA
TTTGCAGCTGCAATGGTTGGTACTGTTGTATTGGTAAAGAGAAAGAAAAACAAAAACAAA
AAGAACAAATCAAAGAAAACCAAAGAACAAATCAACAAAGAAAAAGAAGACAAAAAGAAA
CAAGCAAAAGAAAAGAAAGAAG

>CHF171P.Seq
CAAATATAAATGGTCAATTGTTCCAGAGATTCAATTGGCAAGTTCAAAAGATTATACAGG
TCCAACTTTAAATATTCCTTCAAGTAAATGGGTTTATGATACAACTTATGTTATTACATT
AACTGCAACCTCTGGTAAAGAGACAAACTACGATAATGTTACAGTTATCATTTCAAAGAA
ACCAATCACTGCAGTAATTGATGGAGCCGATAAAGAATTCCCATTAAATACTTTAATAAC
TTTAGATGCTTCAAAATCTTTTGATCCAACAACTACTTCAACAGTTTCCAAAGAACTTGA
TGATTCAACCTCTACACAACAACAATCATTTACTTATGCATGGAGATGTATCAATAAAAG
TGGCAAAGATTGCGGATTAAATTTAGAATCTGAACCAATTATTACATTTAACTCTTCAAG
ATTGAATGTTGGTGTTTTCCATTTCTCTGTTGATGTTACCCCAAATGGTGATGCCACAAG
AAAGTGGTTCTGCTAGTGCCACTGTTACCATTGTAAATACTCCAGTAATTGATGTTATTA
TCTCACCATTACCAACAACCATTGATCCAACTCAAAAATTGGTACTCTATACAGTAGTTA
GTGGTAATGATGCATCTTCATCAAATTTAACCTATGAATGGAATATGCT----------G
AACTGCAATTGGTGAAGAACCATTTACAGTTGCAAGTGATAAAGGTTTCTCGATTACAAC
ATTTAAAACTCTACATATGGAATTAAGTAAGAGTGGTCAATCGATTGGTTTCAAAAAGGA
TGATGAAGGTCAATCTCTTGATGGTGAAAAACTTGCTTTCACTCCAAAATTCAGTATTCC
ATCAAGTGTTTTATCAAAGAATAAATTAAAACAAACCTCTGAAATCACTGGTAAACTAAT
TACAGTTCCATCAAATATTCATTTCAATCATAAATCTGATCAAACCATTTTCCCAAAAAC
TGTTTCACTTGAACTTTTCAATGGTGGCTCTCAATTATCAATCAATGGCCTTAAAGATCA
AATTATCATTGATATGGGAAAACATTCACCACCAAAAGATAAAGAATTTAAATGTGGTTG
GTGGAGCTCCAATAAAAAAGAATGGTTAACAGATGGTTGTTCAACCCATAAAGTAGATGG
TAATATTCAATGTAAATGTAACCATCTTACAGAGTTTGGTTTAATTGAATTTGCAAAATC
CTCTCAAAACATTTGGATCATTATTGGACCAGTAGTTGGTGGTGTTGTATTTGCAGCTGC
AATGGTTGGTACTGTTGTATTGGTAAAGAGAAAGAAAAACAAAAACAAAAAGAACAAATC
AAAGAAAACCAAAGAACAAATCAACAAAGAAAAAGAAGACAAAAAGAAACAAGCAAAAGA
AAAGAAAGAAG