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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CHF157
Library CH
(Link to library)
Clone ID CHF157
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15817-1
Original site URL
Representative seq. ID CHF157P
(Link to Original site)
Representative DNA sequence 
>CHF157 (CHF157Q) /CSM/CH/CHF1-C/CHF157Q.Seq.d/
AAACGAAAAAAATAAAAAAATAGTTTAATAAATAAAAATAAACAAAACAAATAAAAAAAT
AAATAAATAAAAAAAATGAAAACAGTTACATTAGCAACATGTAACTTAAATCAATGGGCA
ATGGATTTCAAAGGAAATCTTGAAAGAATAATAGAATCGATTAATATTGCAAAATCTAAA
GGAGCAAAATATAGATTAGGACCAGAATTAGAAATTTGTGGATATGGTTGTGAAGATCAT
TTTTTTAGAACAAGATACAATGTTACATTGTTGGCAAAGTTTAGCAGTAATATTAAAGGA
TCCAGAGTTAACAAAGGATATTTTAGTTGATGTTGGTATGCCAGTATTACATAAGGATGT
TAGATATAATTGTAGAGTCATATTATTGAATCAAAAGATTTATTTAATACAACCAAAGAA
AGCCATGGCAATGGATGGTAATTATAGGGAAGGTCGTTGGTTTACACCATGGATTAAACC
AAGAGTTGTGGAAACTTTTTATCTTCCAAGGATCATTAGTCAAATCACTGGTCAGGATGA
ATGCCAAATCGGTGATGCAATCATTTCAACACTTGACACAGCCATTTCGTCAGAGACATG
TGAGGAGTTATTTACACCAAATTCACCACATATTCAAATGGGTTTAGATGGGTGTTGAAA
TCTTTAXXXXXXXXXXGTTCTGCAAATTGTGACGAATCATTACGTGGTTATATGACAAAA
TATGATTGTTCATCCGCTGATATCAATCCAATTGGTGGTATGTCAAAAATTGATTTACGT
TCATTCATTGAGTGGGCTGGTAAATTCAGAGATATGAAATCAATTCTCTCTGTACTCACA
GCAACACCAACCGCTGAATTAGAACCAATCACTGAAAACTATACACAATCCGATGAAATT
GATATGGGTATGACCTATGAAGAATTATCAATATTTGGTAAATTAAGAAAAGTCAATCGT
TGTGGTCCAGTTTCAATGTTTGAACGTTTGGTTGCAGATTGGGCACATTTAGAACCATCA
GTTGTGGCTGAAAAAGTTAAACGTTTCTTTTATTATTATGCAATCAATCGTCATAAATTA
ACAACTTTAACACCATCCTATCATGCTGAAGGTTATTCTCCTGATGATAATCGTTATGAT
CATCGTCAATTCCTTTACAATTCAAAATGGGATGTTCAATTTGAAACTATTGATAAAATT
GTATTAAGATTATCTCAAAGACCACAATTAAAAAATACTGTAAACTGTCCAAATCAAGCA
TCATTAACTCAACAATAAAATAAACAAAATAAATAATAATAAAAAAATTA
sequence update 2002.10.25
Translated Amino Acid sequence 
krkk*knslinknkqnk*knk*ikkmktvtlatcnlnqwamdfkgnleriiesiniaksk
gakyrlgpeleicgygcedhffrtrynvtllakfssnikgsrvnkgyfs*cwyasit*gc
*i*l*shiieskdlfnttkeshgngw*l*grslvytmd*tkscgnflsskdh*snhwsg*
mpnr*cnhfnt*hshfvrdm*GVIYTKFTTYSNGFRWVLKSL---

---SANCDESLRGYMTKYDCSSADINPIGGMSKIDLRSFIEWAGKFRDMKSILSVLTATP
TAELEPITENYTQSDEIDMGMTYEELSIFGKLRKVNRCGPVSMFERLVADWAHLEPSVVA
EKVKRFFYYYAINRHKLTTLTPSYHAEGYSPDDNRYDHRQFLYNSKWDVQFETIDKIVLR
LSQRPQLKNTVNCPNQASLTQQ*nkqnk***kn
Translated Amino Acid sequence (All Frames) 
Frame A:
krkk*knslinknkqnk*knk*ikkmktvtlatcnlnqwamdfkgnleriiesiniaksk
gakyrlgpeleicgygcedhffrtrynvtllakfssnikgsrvnkgyfs*cwyasit*gc
*i*l*shiieskdlfnttkeshgngw*l*grslvytmd*tkscgnflsskdh*snhwsg*
mpnr*cnhfnt*hshfvrdm*GVIYTKFTTYSNGFRWVLKSL---

---vlqivtnhyvvi*qnmivhplisiqlvvcqkliyvhslsglvnsei*nqfslysqqh
qpln*nqslktihnpmkliwv*pmknyqylvn*eksivvvqfqclnvwlqighi*nhqlw
lkklnvsfiimqsivin*ql*hhpimlkvillmiivmiivnsftiqngmfnlkllikly*
dylkdhn*kil*tvqikhh*lnnkinkinnnkki

Frame B:
neknkkiv**ikinktnkkink*kk*kqlh*qhvt*ingqwiskeilke**nrlilqnlk
eqnid*dqn*kfvdmvvkiifleqdtmlhcwqslavilkdpeltkdilvdvgmpvlhkdv
ryncrvillnqkiyliqpkkamamdgnyregrwftpwikprvvetfylpriisqitgqde
cqigdaiistldtaissetceelftpnsphiqmgldgc*nl---

---fckl*riitwlydki*lfir*yqsnwwyvkn*ftfih*vgw*iqryeinslcthsnt
nr*irtnh*klytir*n*ygydl*riiniw*ikksqslwssfnv*tfgcrlgtfrtiscg
*ks*tfllllcnqss*innfntilsc*rlfs***sl*sssiplqfkmgcsi*ny**ncik
iiskttikkycklskssiinstik*tk*iiikkl

Frame C:
tkkikk*fnk*k*tkqikk*inkknensyisnm*lksmgngfqrks*knnrid*ycki*r
ski*irtrirnlwiwl*rsff*nkiqcyivgkv*q*y*riqs*qrif*lmlvcqyyirml
diivesyy*ikrfi*ynqrkpwqwmviigkvvglhhglnqelwklfifqgslvkslvrmn
aksvmqsfqhltqpfrqrhvrsylhqihhifkwv*mgveif---

---SANCDESLRGYMTKYDCSSADINPIGGMSKIDLRSFIEWAGKFRDMKSILSVLTATP
TAELEPITENYTQSDEIDMGMTYEELSIFGKLRKVNRCGPVSMFERLVADWAHLEPSVVA
EKVKRFFYYYAINRHKLTTLTPSYHAEGYSPDDNRYDHRQFLYNSKWDVQFETIDKIVLR
LSQRPQLKNTVNCPNQASLTQQ*nkqnk***kn
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHF157 (CHF157Q) /CSM/CH/CHF1-C/CHF157Q.Seq.d/                       2272   0.0
VHC625 (VHC625Q) /CSM/VH/VHC6-B/VHC625Q.Seq.d/                       1183   0.0
VHA707 (VHA707Q) /CSM/VH/VHA7-A/VHA707Q.Seq.d/                       1183   0.0
CHS208 (CHS208Q) /CSM/CH/CHS2-A/CHS208Q.Seq.d/                       1183   0.0
CHR543 (CHR543Q) /CSM/CH/CHR5-B/CHR543Q.Seq.d/                       1183   0.0
CHR356 (CHR356Q) /CSM/CH/CHR3-C/CHR356Q.Seq.d/                       1183   0.0
CHQ803 (CHQ803Q) /CSM/CH/CHQ8-A/CHQ803Q.Seq.d/                       1183   0.0
CHQ189 (CHQ189Q) /CSM/CH/CHQ1-D/CHQ189Q.Seq.d/                       1183   0.0
CHP772 (CHP772Q) /CSM/CH/CHP7-C/CHP772Q.Seq.d/                       1183   0.0
CHI337 (CHI337Q) /CSM/CH/CHI3-B/CHI337Q.Seq.d/                       1183   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AX489232|AX489232.1 Sequence 6532 from Patent WO02053728.          92   2e-22  4
BZ294214|BZ294214.1 CG0770.f1 Candida glabrata Random Genomic Library Candida glabrata genomic clone CG0770, DNA sequence.    52   4e-11  3
U10556|U10556.1 Saccharomyces cerevisiae chromosome VIII cosmid 9205.    52   6e-09  5
AL392865|AL392865.1 T7 end of clone AR0AA007D04 of library AR0AA from strain CBS 732 of Zygosaccharomyces rouxii.    54   8e-09  2
CR380956|CR380956.1 Candida glabrata strain CBS138 chromosome J complete sequence.    52   1e-07  20
CR382124|CR382124.1 Kluyveromyces lactis strain NRRL Y-1140 chromosome D of strain NRRL  Y-1140 of Kluyveromyces lactis.    52   4e-07  2
CR382133|CR382133.1 Debaryomyces hansenii chromosome A of strain CBS767 of Debaryomyces hansenii.    52   5e-07  20
AL023704|AL023704.2 S.pombe chromosome III cosmid c553.            38   1e-06  7
AL437572|AL437572.1 T3 end of clone BC0AA010B06 of library BC0AA from strain CBS 767 of Debaryomyces hansenii.    52   1e-05  2
CB395141|CB395141.1 OSTR149E3_1 AD-wrmcDNA Caenorhabditis elegans cDNA, mRNA sequence.    54   3e-04  2
dna update 2004. 9.27
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

BC161121_1(BC161121|pid:none) Xenopus tropicalis hypothetical pr...   220   2e-65
(Q3ZBF0) RecName: Full=Glutamine-dependent NAD(+) synthetase;   ...   210   9e-65
AP008213_307(AP008213|pid:none) Oryza sativa (japonica cultivar-...   242   2e-62
BC038016_1(BC038016|pid:none) Mus musculus NAD synthetase 1, mRN...   218   5e-62
AE016820_727(AE016820|pid:none) Ashbya gossypii (= Eremothecium ...   236   7e-62
CR380956_461(CR380956|pid:none) Candida glabrata strain CBS138 c...   235   9e-62
BC161832_1(BC161832|pid:none) Rattus norvegicus NAD synthetase 1...   219   3e-61
CR382124_545(CR382124|pid:none) Kluyveromyces lactis strain NRRL...   238   4e-61
(Q812E8) RecName: Full=Glutamine-dependent NAD(+) synthetase;   ...   219   7e-61
CP000589_178(CP000589|pid:none) Ostreococcus lucimarinus CCE9901...   235   2e-60
protein update 2009. 3.31
PSORT 

 psg: 0.82  gvh: 0.40  alm: 0.47  top: 0.53  tms: 0.00  mit: 0.34  mip: 0.04
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.50  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.50
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 32.0 %: cytoplasmic
	 12.0 %: mitochondrial
	  4.0 %: Golgi
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for CHF157 is nuc
5' end seq. ID CHF157F
5' end seq. 
>CHF157F.Seq
AAACGAAAAAAATAAAAAAATAGTTTAATAAATAAAAATAAACAAAACAAATAAAAAAAT
AAATAAATAAAAAAAATGAAAACAGTTACATTAGCAACATGTAACTTAAATCAATGGGCA
ATGGATTTCAAAGGAAATCTTGAAAGAATAATAGAATCGATTAATATTGCAAAATCTAAA
GGAGCAAAATATAGATTAGGACCAGAATTAGAAATTTGTGGATATGGTTGTGAAGATCAT
TTTTTTAGAACAAGATACAATGTTACATTGTTGGCAAAGTTTAGCAGTAATATTAAAGGA
TCCAGAGTTAACAAAGGATATTTTAGTTGATGTTGGTATGCCAGTATTACATAAGGATGT
TAGATATAATTGTAGAGTCATATTATTGAATCAAAAGATTTATTTAATACAACCAAAGAA
AGCCATGGCAATGGATGGTAATTATAGGGAAGGTCGTTGGTTTACACCATGGATTAAACC
AAGAGTTGTGGAAACTTTTTATCTTCCAAGGATCATTAGTCAAATCACTGGTCAGGATGA
ATGCCAAATCGGTGATGCAATCATTTCAACACTTGACACAGCCATTTCGTCAGAGACATG
TGAGGAGTTATTTACACCAAATTCACCACATATTCAAATGGGTTTAGATGGGTGTTGAAA
TCTTTANNNNNNNNNN
Length of 5' end seq. 676
3' end seq. ID CHF157Z
3' end seq. 
>CHF157Z.Seq
NNNNNNNNNNGTTCTGCAAATTGTGACGAATCATTACGTGGTTATATGACAAAATATGAT
TGTTCATCCGCTGATATCAATCCAATTGGTGGTATGTCAAAAATTGATTTACGTTCATTC
ATTGAGTGGGCTGGTAAATTCAGAGATATGAAATCAATTCTCTCTGTACTCACAGCAACA
CCAACCGCTGAATTAGAACCAATCACTGAAAACTATACACAATCCGATGAAATTGATATG
GGTATGACCTATGAAGAATTATCAATATTTGGTAAATTAAGAAAAGTCAATCGTTGTGGT
CCAGTTTCAATGTTTGAACGTTTGGTTGCAGATTGGGCACATTTAGAACCATCAGTTGTG
GCTGAAAAAGTTAAACGTTTCTTTTATTATTATGCAATCAATCGTCATAAATTAACAACT
TTAACACCATCCTATCATGCTGAAGGTTATTCTCCTGATGATAATCGTTATGATCATCGT
CAATTCCTTTACAATTCAAAATGGGATGTTCAATTTGAAACTATTGATAAAATTGTATTA
AGATTATCTCAAAGACCACAATTAAAAAATACTGTAAACTGTCCAAATCAAGCATCATTA
ACTCAACAATAAAATAAACAAAATAAATAATAATAAAAAAATTA
Length of 3' end seq. 644
Connected seq. ID CHF157P
Connected seq. 
>CHF157P.Seq
AAACGAAAAAAATAAAAAAATAGTTTAATAAATAAAAATAAACAAAACAAATAAAAAAAT
AAATAAATAAAAAAAATGAAAACAGTTACATTAGCAACATGTAACTTAAATCAATGGGCA
ATGGATTTCAAAGGAAATCTTGAAAGAATAATAGAATCGATTAATATTGCAAAATCTAAA
GGAGCAAAATATAGATTAGGACCAGAATTAGAAATTTGTGGATATGGTTGTGAAGATCAT
TTTTTTAGAACAAGATACAATGTTACATTGTTGGCAAAGTTTAGCAGTAATATTAAAGGA
TCCAGAGTTAACAAAGGATATTTTAGTTGATGTTGGTATGCCAGTATTACATAAGGATGT
TAGATATAATTGTAGAGTCATATTATTGAATCAAAAGATTTATTTAATACAACCAAAGAA
AGCCATGGCAATGGATGGTAATTATAGGGAAGGTCGTTGGTTTACACCATGGATTAAACC
AAGAGTTGTGGAAACTTTTTATCTTCCAAGGATCATTAGTCAAATCACTGGTCAGGATGA
ATGCCAAATCGGTGATGCAATCATTTCAACACTTGACACAGCCATTTCGTCAGAGACATG
TGAGGAGTTATTTACACCAAATTCACCACATATTCAAATGGGTTTAGATGGGTGTTGAAA
TCTTTA----------GTTCTGCAAATTGTGACGAATCATTACGTGGTTATATGACAAAA
TATGATTGTTCATCCGCTGATATCAATCCAATTGGTGGTATGTCAAAAATTGATTTACGT
TCATTCATTGAGTGGGCTGGTAAATTCAGAGATATGAAATCAATTCTCTCTGTACTCACA
GCAACACCAACCGCTGAATTAGAACCAATCACTGAAAACTATACACAATCCGATGAAATT
GATATGGGTATGACCTATGAAGAATTATCAATATTTGGTAAATTAAGAAAAGTCAATCGT
TGTGGTCCAGTTTCAATGTTTGAACGTTTGGTTGCAGATTGGGCACATTTAGAACCATCA
GTTGTGGCTGAAAAAGTTAAACGTTTCTTTTATTATTATGCAATCAATCGTCATAAATTA
ACAACTTTAACACCATCCTATCATGCTGAAGGTTATTCTCCTGATGATAATCGTTATGAT
CATCGTCAATTCCTTTACAATTCAAAATGGGATGTTCAATTTGAAACTATTGATAAAATT
GTATTAAGATTATCTCAAAGACCACAATTAAAAAATACTGTAAACTGTCCAAATCAAGCA
TCATTAACTCAACAATAAAATAAACAAAATAAATAATAATAAAAAAATTA
Length of connected seq. 1300
Full length Seq ID -
Full length Seq. -
Length of full length seq. -