>CHF147 (CHF147Q) /CSM/CH/CHF1-B/CHF147Q.Seq.d/
XXXXXXXXXXAAAAAAAAAAAAAACAACCAAAATATTATATTATTTTTTGTAAATATAAA
TTAAAAAAATGAAATTCCAACATACATTTATTGCATTATTATCACTATTAACATATGCAA
ATGCATATGATTATTTCACAACAACATTGGCCAATCAAAATCCAGTTTGTGCTTCAGTAG
ATGTCATACAAAATGTTTGTACTGAGGTTTGTGGTAGATTTGTCCGTTACATTCCTGATG
CTACCAATACAAATCAATTCACTTTTGCCGAATATACTACAAACCAATGTACTGTTCAAG
TAACACCAGCTGTAACAAATACATTTACTTGCGCTGATCAAACTTCTTCACATGCACTCG
GTTCTGATTGGAGTGGAGTTTGTAAAATCACTGCCACACCCGCACCAACAGTTACACCAA
CAGTTACACCAACAGTTACACCAACAGTAACACCAACACCAACAAATACACCAAATCCAA
CCCCATCCCAAACATCAACAACAACTGGTTCTGCTTCAACTGTTGTTGCAAGTTTATCAC
TCATTATTTTCTCAATGATTCTTTNCCCTTTGTTAATTA

---kkkkttkilyyfl*i*IKKMKFQHTFIALLSLLTYANAYDYFTTTLANQNPVCASVD
VIQNVCTEVCGRFVRYIPDATNTNQFTFAEYTTNQCTVQVTPAVTNTFTCADQTSSHALG
SDWSGVCKITATPAPTVTPTVTPTVTPTVTPTPTNTPNPTPSQTSTTTGSASTVVASLSL
IIFSMILXPLLI

Frame A:
---kkkkttkilyyfl*i*IKKMKFQHTFIALLSLLTYANAYDYFTTTLANQNPVCASVD
VIQNVCTEVCGRFVRYIPDATNTNQFTFAEYTTNQCTVQVTPAVTNTFTCADQTSSHALG
SDWSGVCKITATPAPTVTPTVTPTVTPTVTPTPTNTPNPTPSQTSTTTGSASTVVASLSL
IIFSMILXPLLI


Frame B:
---kkkkqpkyyiifckyklkk*nsnihllhyyhy*hmqmhmiisqqhwpikiqfvlq*m
sykmfvlrfvvdlsvtflmlpiqinsllpnilqtnvlfk*hql*qihllalikllhmhsv
ligvefvkslphphqqlhqqlhqqlhqq*hqhqqihqiqphpkhqqqlvllqlllqvyhs
lfsq*ffxlc*l


Frame C:
---kkknnqniilffvnin*kneiptyiyciiitinickci*lfhnnigqsksslcfsrc
htkcly*glw*icplhs*cyqyksihfcriyykpmycssntscnkyiylr*snfftctrf
*lewsl*nhchtrtnsytnsytnsytnsntntnkytksnpipninnnwfcfnccckfith
yflndsxpfvn

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHF147 (CHF147Q) /CSM/CH/CHF1-B/CHF147Q.Seq.d/                       1053   0.0
SSI759 (SSI759Q) /CSM/SS/SSI7-C/SSI759Q.Seq.d/                       1041   0.0
SSI680 (SSI680Q) /CSM/SS/SSI6-D/SSI680Q.Seq.d/                       1041   0.0
SSC554 (SSC554Q) /CSM/SS/SSC5-C/SSC554Q.Seq.d/                       1041   0.0
SLG216 (SLG216Q) /CSM/SL/SLG2-A/SLG216Q.Seq.d/                       1041   0.0
CHL273 (CHL273Q) /CSM/CH/CHL2-D/CHL273Q.Seq.d/                       1039   0.0
CHL169 (CHL169Q) /CSM/CH/CHL1-C/CHL169Q.Seq.d/                       1039   0.0
SHB196 (SHB196Q) /CSM/SH/SHB1-D/SHB196Q.Seq.d/                       1035   0.0
SHD636 (SHD636Q) /CSM/SH/SHD6-B/SHD636Q.Seq.d/                       1033   0.0
AHM287 (AHM287Q) /CSM/AH/AHM2-D/AHM287Q.Seq.d/                       1031   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

X15980|X15980.1 Dictyostelium discoideum D19 gene 5'-flanking region.   973   0.0    2
M20909|M20909.1 Dictyostelium discoideum cell surface glycoprotein PsA precursor (D19) gene, complete cds.   973   0.0    2
BD075065|BD075065.1 Expression of gonadotropin in Dictyostelium.   111   1e-20  1
A98322|A98322.1 Sequence 1 from Patent WO9913081.                 111   1e-20  1
A21625|A21625.1 expression vector pMUW1630.                       105   8e-19  1
U20661|U20661.1 Dictyostelium discoideum unknown internal repeat protein gene, complete cds, and unknown orf1, orf2 and orf3 genes, partial cds.    66   7e-07  1
AF224509|AF224509.3 Bacteroides cellulosolvens cellulosomal scaffoldin precursor (cipBc), cellulosomal anchoring scaffoldin B (scaB), and sodium/calcium exchanger protein genes, complete cds.    44   5e-04  4
U16308|U16308.1 Caldocellum saccharolyticum endoglucanase/mannanase (celC) pseudogene, complete cds.    54   7e-04  2
AC146587|AC146587.20 Medicago truncatula clone mth2-144j8, complete sequence.    38   0.001  5
AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.    46   0.001  9

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54LC3) RecName: Full=Ponticulin-like protein C2; Flags: Precur...    53   5e-06
A98322_1(A98322|pid:none) Sequence 1 from Patent WO9913081.            41   0.026
(P36170) RecName: Full=Putative uncharacterized protein YKR102W;...    33   4.1
CP000441_1555(CP000441|pid:none) Burkholderia ambifaria AMMD chr...    32   9.1
CP001322_2279(CP001322|pid:none) Desulfatibacillum alkenivorans ...    32   9.1

 psg: 0.96  gvh: 0.78  alm: 0.24  top: 0.17  tms: 0.07  mit: 0.32  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.00  gpi: 1.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 1.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 36.0 %: plasma membrane
	 32.0 %: extracellular, including cell wall
	 20.0 %: endoplasmic reticulum
	 12.0 %: Golgi

>> prediction for CHF147 is pla

>CHF147Z.Seq
NNNNNNNNNNAAAAAAAAAAAAAACAACCAAAATATTATATTATTTTTTGTAAATATAAA
TTAAAAAAATGAAATTCCAACATACATTTATTGCATTATTATCACTATTAACATATGCAA
ATGCATATGATTATTTCACAACAACATTGGCCAATCAAAATCCAGTTTGTGCTTCAGTAG
ATGTCATACAAAATGTTTGTACTGAGGTTTGTGGTAGATTTGTCCGTTACATTCCTGATG
CTACCAATACAAATCAATTCACTTTTGCCGAATATACTACAAACCAATGTACTGTTCAAG
TAACACCAGCTGTAACAAATACATTTACTTGCGCTGATCAAACTTCTTCACATGCACTCG
GTTCTGATTGGAGTGGAGTTTGTAAAATCACTGCCACACCCGCACCAACAGTTACACCAA
CAGTTACACCAACAGTTACACCAACAGTAACACCAACACCAACAAATACACCAAATCCAA
CCCCATCCCAAACATCAACAACAACTGGTTCTGCTTCAACTGTTGTTGCAAGTTTATCAC
TCATTATTTTCTCAATGATTCTTTNCCCTTTGTTAATTA