NBDC - アーカイブトップ - ヘルプ
データベースの説明 | データ項目の説明 | 利用許諾 | ヘルプ
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
Description of this DB | Description of data items | Download | License
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CHF136
Library CH
(Link to library)
Clone ID CHF136
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID CHF136P
(Link to Original site)
Representative DNA sequence 
>CHF136 (CHF136Q) /CSM/CH/CHF1-B/CHF136Q.Seq.d/
TGGCCTACTGGAAAATAAAATTCATTTAATAATAATAATTTAATTTTTTTCATAGTATTC
AAAGATATAATGAAAAACTTTTATTATTTTATATTAATTTTATTATTTTTTAATGAAGTT
TGCTATTCATTAAAAGATGGTGAAATTAAACTTCATTTAATTCCACATTCACATTGTGAT
TCTGGATGGACATCAACAATGAACGAATATTATATGGGTCAAGTTAAAAGTATTATATCA
TCAATGGTTCAATCATTAAATGTTGAATCAAATCCACCTAGAAAATTTGTTTGGTCTGAA
ATTGGATTTTTAGAACAATGGTGGGATGATATGCCAATTGAAATTAAAAATGATTTTATA
AAACATGTTAAAAATGATAGAATTGAATTTGTAAATGGTGGATGGGTAATGAATGATGAA
GCATGTGCAAGTTTAGAAAGTGTAATTAGACAATTATCAAATGGACATAAATTTATTAGA
GAGAAATTTGGAAAACAACCAGAGAGTGGATGGCAAATTGATCCATTTGGACATTCATCA
TTGACACCAACATTACAAGCTCAATTTGGTTATAAACATGTTGTATTGAATCGTATTCAC
TATGAATTGAAAAAGAAATTTAAAGAAGAAAAGAATTTACAATTTAAXXXXXXXXXXATC
AGTTGAAGTTGGTAGAGAAACTCAAAAGTTATTATCCAACTATTAATTCAGTTTATATCG
AATCTCAAAGTACTGGAAAAAGATTTGTTTGTAATAATGATAGATCAAGAGGTGTTTCAA
GTCAAGGTCAAGGTTGCTTGGAAATGGCATTACATAGAAGTTTAACTTATGAAGATGGTA
AAGGTTTAGAAATTCCTGCAATTGATGAATCCTCAATTAATGCACGTTTTGAATGTTATT
TAGATGAAGTACCATCAAATTCACAACAATCGAATGGTGGTGGTGGTGGTGATGATATTA
GAAAACAATCAATTAATTATCAACATAAATTCCAAATTTATCAAGGTCAAGATTCTTCTT
ATATGTCTTCAAAATCTTTTATGTTAAAACCATTACCAGAGTTTATTCATATTCTATCAA
TGGAAAGATCTGGTCCTCGTTCAATTAAATTAAGAATTCATAATATTGAAAATAATAATC
AATCTCCAATCACTTTTGATTTAAATGGCTTATTCTCTTTTATCAAATCAATTAAATCAA
TTAAAGAATATAATTTATCTTTAATCAATAGATTCGTTGATAATAATATTGATAATATTA
TTTCTTCTCATCGTTCAATTGTTGGAAAAAATCTTTTCCCTATTAAAGATACTCCAACTC
GTTTCAACCCAATTAATACAAAACAAACTAAAATTACCTTATATCCATCAGAAATTAAAG
CAATNAAATTACTTATCATTAAAATTA
sequence update 2002.10.25
Translated Amino Acid sequence 
wptgk*NSFNNNNLIFFIVFKDIMKNFYYFILILLFFNEVCYSLKDGEIKLHLIPHSHCD
SGWTSTMNEYYMGQVKSIISSMVQSLNVESNPPRKFVWSEIGFLEQWWDDMPIEIKNDFI
KHVKNDRIEFVNGGWVMNDEACASLESVIRQLSNGHKFIREKFGKQPESGWQIDPFGHSS
LTPTLQAQFGYKHVVLNRIHYELKKKFKEEKNLQF---

---QLKLVEKLKSYYPTINSVYIESQSTGKRFVCNNDRSRGVSSQGQGCLEMALHRSLTY
EDGKGLEIPAIDESSINARFECYLDEVPSNSQQSNGGGGGDDIRKQSINYQHKFQIYQGQ
DSSYMSSKSFMLKPLPEFIHILSMERSGPRSIKLRIHNIENNNQSPITFDLNGLFSFIKS
IKSIKEYNLSLINRFVDNNIDNIISSHRSIVGKNLFPIKDTPTRFNPINTKQTKITLYPS
EIKAXKLLIIKI
Translated Amino Acid sequence (All Frames) 
Frame A:
wptgk*NSFNNNNLIFFIVFKDIMKNFYYFILILLFFNEVCYSLKDGEIKLHLIPHSHCD
SGWTSTMNEYYMGQVKSIISSMVQSLNVESNPPRKFVWSEIGFLEQWWDDMPIEIKNDFI
KHVKNDRIEFVNGGWVMNDEACASLESVIRQLSNGHKFIREKFGKQPESGWQIDPFGHSS
LTPTLQAQFGYKHVVLNRIHYELKKKFKEEKNLQF---

---is*sw*rnskviiqlliqfisnlkvlekdlfvimidqevfqvkvkvawkwhyiev*l
mkmvkv*kflqlmnpqlmhvlnvi*mkyhqihnnrmvvvvvmilennqliininskfikv
killiclqnllc*nhyqslfifyqwkdlvlvqln*efiilkiiinlqslli*maysllsn
qlnqlkniiyl*sidsliiiliilfllivqllekifsllkilqlvstqliqnklklpyih
qklkqxnylslkl

Frame B:
gllenkihliiii*ffs*yski**ktfiily*fyyflmkfaih*kmvklnfi*fhihivi
ldghqq*tniiwvklkvlyhqwfnh*mlnqihlenlfglkldf*nnggmicqlklkmil*
nmlkmielnl*mvdg**mmkhvqv*kv*ldnyqmdinllernlennqrvdgklihldihh
*hqhyklnlvinmly*ivftmn*krnlkkkriynl---

---svevgretqkllsny*fslyriskywkkicl****ikrcfksrsrllgngit*kfnl
*rw*rfrnscn**iln*ctf*mlfr*stikfttiewwwww**y*ktin*lst*ipnlsrs
rfflyvfkifyvktitrvysysingkiwssfn*ikns*y*k**sisnhf*fkwlilfyqi
n*in*ri*fifnq*ir***y**yyffssfncwkksfpy*rysnsfqpn*yktn*nylisi
rn*snxityh*n

Frame C:
aywkikfi****fnffhsiqryneklllfyinfiif**sllfikrw*n*tsfnstftl*f
wmdinnerilygss*kyyiingsiikc*ikst*kiclv*nwifrtmvg*yan*n*k*fyk
tc*k**n*ickwwmgne**smckfrkcn*tiikwt*iy*reiwkttrewman*siwtfii
dtnitssiwl*tcciesysl*iekei*rrkefti*---

---QLKLVEKLKSYYPTINSVYIESQSTGKRFVCNNDRSRGVSSQGQGCLEMALHRSLTY
EDGKGLEIPAIDESSINARFECYLDEVPSNSQQSNGGGGGDDIRKQSINYQHKFQIYQGQ
DSSYMSSKSFMLKPLPEFIHILSMERSGPRSIKLRIHNIENNNQSPITFDLNGLFSFIKS
IKSIKEYNLSLINRFVDNNIDNIISSHRSIVGKNLFPIKDTPTRFNPINTKQTKITLYPS
EIKAXKLLIIKI
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHL340 (SHL340Q) /CSM/SH/SHL3-B/SHL340Q.Seq.d/                       1061   0.0
SHL163 (SHL163Q) /CSM/SH/SHL1-C/SHL163Q.Seq.d/                       1061   0.0
SHK365 (SHK365Q) /CSM/SH/SHK3-C/SHK365Q.Seq.d/                       1061   0.0
SHJ609 (SHJ609Q) /CSM/SH/SHJ6-A/SHJ609Q.Seq.d/                       1061   0.0
SHI833 (SHI833Q) /CSM/SH/SHI8-B/SHI833Q.Seq.d/                       1061   0.0
SHI789 (SHI789Q) /CSM/SH/SHI7-D/SHI789Q.Seq.d/                       1061   0.0
SHI645 (SHI645Q) /CSM/SH/SHI6-B/SHI645Q.Seq.d/                       1061   0.0
SHI325 (SHI325Q) /CSM/SH/SHI3-B/SHI325Q.Seq.d/                       1061   0.0
SHI290 (SHI290Q) /CSM/SH/SHI2-D/SHI290Q.Seq.d/                       1061   0.0
SHI182 (SHI182Q) /CSM/SH/SHI1-D/SHI182Q.Seq.d/                       1061   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AE017263|AE017263.1 Mesoplasma florum L1 complete genome.          34   7e-16  33
CR382400|CR382400.1 Plasmodium falciparum chromosome 6, complete sequence; segment 3/5.    32   1e-14  25
AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.    38   4e-13  21
AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.    40   7e-13  18
AL844506|AL844506.1 Plasmodium falciparum chromosome  7.           40   6e-11  22
AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.    40   7e-11  16
AF250284|AF250284.1 Amsacta moorei entomopoxvirus, complete genome.    44   1e-10  17
AF063866|AF063866.1 Melanoplus sanguinipes entomopoxvirus, complete genome.    38   1e-10  19
AL929357|AL929357.1 Plasmodium falciparum strain 3D7, chromosome 9; segment 3/5.    34   2e-10  22
AE014816|AE014816.1 Plasmodium falciparum 3D7 chromosome 14 section 1 of 13 of the complete sequence.    38   9e-10  19
dna update 2004. 9.26
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54YC4) RecName: Full=Alpha-mannosidase D;          EC=3.2.1.24...   147   1e-33
(Q54YF7) RecName: Full=Alpha-mannosidase B;          EC=3.2.1.24...   145   2e-33
BC142656_1(BC142656|pid:none) Homo sapiens mannosidase, alpha, c...   140   1e-31
AB011474_23(AB011474|pid:none) Arabidopsis thaliana genomic DNA,...   139   2e-31
(P27046) RecName: Full=Alpha-mannosidase 2;          EC=3.2.1.11...   139   2e-31
AL391146_7(AL391146|pid:none) Arabidopsis thaliana DNA chromosom...   139   3e-31
(P28494) RecName: Full=Alpha-mannosidase 2;          EC=3.2.1.11...   138   5e-31
M24353_1(M24353|pid:none) Rattus norvegicus alpha-mannosidase II...   138   5e-31
AC148217_12(AC148217|pid:none) Medicago truncatula clone mth2-20...   137   7e-31
AK161799_1(AK161799|pid:none) Mus musculus 13 days embryo male t...   137   1e-30
protein update 2009. 3.31
PSORT 

 psg: 0.94  gvh: 0.66  alm: 0.48  top: 0.47  tms: 0.00  mit: 0.29  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: extracellular, including cell wall
	 20.0 %: cytoplasmic
	 12.0 %: nuclear
	  8.0 %: mitochondrial
	  8.0 %: vacuolar
	  8.0 %: endoplasmic reticulum
	  4.0 %: Golgi

>> prediction for CHF136 is exc
5' end seq. ID CHF136F
5' end seq. 
>CHF136F.Seq
TGGCCTACTGGAAAATAAAATTCATTTAATAATAATAATTTAATTTTTTTCATAGTATTC
AAAGATATAATGAAAAACTTTTATTATTTTATATTAATTTTATTATTTTTTAATGAAGTT
TGCTATTCATTAAAAGATGGTGAAATTAAACTTCATTTAATTCCACATTCACATTGTGAT
TCTGGATGGACATCAACAATGAACGAATATTATATGGGTCAAGTTAAAAGTATTATATCA
TCAATGGTTCAATCATTAAATGTTGAATCAAATCCACCTAGAAAATTTGTTTGGTCTGAA
ATTGGATTTTTAGAACAATGGTGGGATGATATGCCAATTGAAATTAAAAATGATTTTATA
AAACATGTTAAAAATGATAGAATTGAATTTGTAAATGGTGGATGGGTAATGAATGATGAA
GCATGTGCAAGTTTAGAAAGTGTAATTAGACAATTATCAAATGGACATAAATTTATTAGA
GAGAAATTTGGAAAACAACCAGAGAGTGGATGGCAAATTGATCCATTTGGACATTCATCA
TTGACACCAACATTACAAGCTCAATTTGGTTATAAACATGTTGTATTGAATCGTATTCAC
TATGAATTGAAAAAGAAATTTAAAGAAGAAAAGAATTTACAATTTAANNNNNNNNNN
Length of 5' end seq. 657
3' end seq. ID CHF136Z
3' end seq. 
>CHF136Z.Seq
NNNNNNNNNNATCAGTTGAAGTTGGTAGAGAAACTCAAAAGTTATTATCCAACTATTAAT
TCAGTTTATATCGAATCTCAAAGTACTGGAAAAAGATTTGTTTGTAATAATGATAGATCA
AGAGGTGTTTCAAGTCAAGGTCAAGGTTGCTTGGAAATGGCATTACATAGAAGTTTAACT
TATGAAGATGGTAAAGGTTTAGAAATTCCTGCAATTGATGAATCCTCAATTAATGCACGT
TTTGAATGTTATTTAGATGAAGTACCATCAAATTCACAACAATCGAATGGTGGTGGTGGT
GGTGATGATATTAGAAAACAATCAATTAATTATCAACATAAATTCCAAATTTATCAAGGT
CAAGATTCTTCTTATATGTCTTCAAAATCTTTTATGTTAAAACCATTACCAGAGTTTATT
CATATTCTATCAATGGAAAGATCTGGTCCTCGTTCAATTAAATTAAGAATTCATAATATT
GAAAATAATAATCAATCTCCAATCACTTTTGATTTAAATGGCTTATTCTCTTTTATCAAA
TCAATTAAATCAATTAAAGAATATAATTTATCTTTAATCAATAGATTCGTTGATAATAAT
ATTGATAATATTATTTCTTCTCATCGTTCAATTGTTGGAAAAAATCTTTTCCCTATTAAA
GATACTCCAACTCGTTTCAACCCAATTAATACAAAACAAACTAAAATTACCTTATATCCA
TCAGAAATTAAAGCAATNAAATTACTTATCATTAAAATTA
Length of 3' end seq. 760
Connected seq. ID CHF136P
Connected seq. 
>CHF136P.Seq
TGGCCTACTGGAAAATAAAATTCATTTAATAATAATAATTTAATTTTTTTCATAGTATTC
AAAGATATAATGAAAAACTTTTATTATTTTATATTAATTTTATTATTTTTTAATGAAGTT
TGCTATTCATTAAAAGATGGTGAAATTAAACTTCATTTAATTCCACATTCACATTGTGAT
TCTGGATGGACATCAACAATGAACGAATATTATATGGGTCAAGTTAAAAGTATTATATCA
TCAATGGTTCAATCATTAAATGTTGAATCAAATCCACCTAGAAAATTTGTTTGGTCTGAA
ATTGGATTTTTAGAACAATGGTGGGATGATATGCCAATTGAAATTAAAAATGATTTTATA
AAACATGTTAAAAATGATAGAATTGAATTTGTAAATGGTGGATGGGTAATGAATGATGAA
GCATGTGCAAGTTTAGAAAGTGTAATTAGACAATTATCAAATGGACATAAATTTATTAGA
GAGAAATTTGGAAAACAACCAGAGAGTGGATGGCAAATTGATCCATTTGGACATTCATCA
TTGACACCAACATTACAAGCTCAATTTGGTTATAAACATGTTGTATTGAATCGTATTCAC
TATGAATTGAAAAAGAAATTTAAAGAAGAAAAGAATTTACAATTTAA----------ATC
AGTTGAAGTTGGTAGAGAAACTCAAAAGTTATTATCCAACTATTAATTCAGTTTATATCG
AATCTCAAAGTACTGGAAAAAGATTTGTTTGTAATAATGATAGATCAAGAGGTGTTTCAA
GTCAAGGTCAAGGTTGCTTGGAAATGGCATTACATAGAAGTTTAACTTATGAAGATGGTA
AAGGTTTAGAAATTCCTGCAATTGATGAATCCTCAATTAATGCACGTTTTGAATGTTATT
TAGATGAAGTACCATCAAATTCACAACAATCGAATGGTGGTGGTGGTGGTGATGATATTA
GAAAACAATCAATTAATTATCAACATAAATTCCAAATTTATCAAGGTCAAGATTCTTCTT
ATATGTCTTCAAAATCTTTTATGTTAAAACCATTACCAGAGTTTATTCATATTCTATCAA
TGGAAAGATCTGGTCCTCGTTCAATTAAATTAAGAATTCATAATATTGAAAATAATAATC
AATCTCCAATCACTTTTGATTTAAATGGCTTATTCTCTTTTATCAAATCAATTAAATCAA
TTAAAGAATATAATTTATCTTTAATCAATAGATTCGTTGATAATAATATTGATAATATTA
TTTCTTCTCATCGTTCAATTGTTGGAAAAAATCTTTTCCCTATTAAAGATACTCCAACTC
GTTTCAACCCAATTAATACAAAACAAACTAAAATTACCTTATATCCATCAGAAATTAAAG
CAATNAAATTACTTATCATTAAAATTA
Length of connected seq. 1397
Full length Seq ID -
Full length Seq. -
Length of full length seq. -