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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CHE648
Library CH
(Link to library)
Clone ID CHE648
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16524-1
Original site URL
Representative seq. ID CHE648P
(Link to Original site)
Representative DNA sequence 
>CHE648 (CHE648Q) /CSM/CH/CHE6-B/CHE648Q.Seq.d/
TTACTTCATGTGCACTCGGTGCAAACAATGGTAATTTCTTTAGTTGTGATATCTCTTCTC
CAGTTGATACTTNAATAGGCCTTCATGTAGTTCTCTTTTCTCTTCTGCAACTTTACAAAC
TTTGGGTAGTTCTCCAAGATGTGAATATACTGCTTCAAACATTCTAATGGTTACATTGGG
TAGTGGTGAAAGTGTATCAGTTGGCCAACAATTGGAATTTTCTCAAGGTGGAATCACTTC
AAAAGATGGTAGTAAGAATAATTATCAAACTATAACATTGGAACCACCACAAGATTCAAC
TCCAGTGGTAGATATTAAAGCTCCAAATGTTTTAGATTCATGTAGTCCATTGGTATTAGA
TGCTAGTCATTCAAAAGGTGGTAATGGAAAAGCATTAACCTATGATTGGTCGGTTAGTGG
GGCAGATACAAACACTCAATTAAATAATCTATTGGATCAAAATAATGCAACCATAACAAT
TCCAAATGATCTTTTGTCATCTGGTCAAACTTATACTTTTACACTTAAAATCACAAATTG
GTTAAAGAAATCTGATAGCGCAAGTGTTTCAGTAACAAAGAAAXXXXXXXXXXTTGGCAG
ATGGTATTCTTCAAAGAACTGCAATTGGTGAAGAACCATTTACAGTTGCAAGTGATAAAG
GTTTCTCGATTACAACATTTAAAACTCTACATATGGAATTAAGTAAGAGTGGTCAATCGA
TTGGTTTCAAAAAGGATGATGAAGGTCAATCTCTTGATGGTGAAAAACTTGCTTTCACTC
CAAAATTCAGTATTCCATCAAGTGTTTTATCAAAGAATAAATTAAAACAAACCTCTGAAA
TCACTGGTAAACTAATTACAGTTCCATCAAATATTCATTTCAATCATAAATCTGATCAAA
CCATTTTCCCAAAAACTGTTTCACTTGAACTTTTCAATGGTGGCTCTCAATTATCAATCA
ATGGCCTTAAAGATCAAATTATCATTGATATGGGAAAACATTCACCACCAAAAGATAAAG
AATTTAAATGTGGTTGGTGGAGCTCCAATAAAAAAGAATGGTTAACAGATGGTTGTTCAA
CCCATAAAGTAGATGGTAATATTCAATGTAAATGTAACCATCTTACAGAGTTTGGTTTAA
TTGAATTTGCAAAATCCTCTCAAAACATTTGGATCATTATTGGACCAGTAGTTGGTGGTG
TTGTATTTGCAGCTGCAATGGTTGGTACTGTTGTATTGGTAAAGAGAAAGAAAAACAAAA
ACAAAAAGAACAAATCAAAGAAAACCAAAGAACTAATCAACAAAGAAAAAGAAGACAAAA
AGAAACAAGCAAAAGAAAAGAAAGAAGCNNATAAATGGAAAAAGAAA
sequence update 2002.10.25
Translated Amino Acid sequence 
yfmctrckqw*fl*l*ylfss*YXNRPSCSSLFSSATLQTLGSSPRCEYTASNILMVTLG
SGESVSVGQQLEFSQGGITSKDGSKNNYQTITLEPPQDSTPVVDIKAPNVLDSCSPLVLD
ASHSKGGNGKALTYDWSVSGADTNTQLNNLLDQNNATITIPNDLLSSGQTYTFTLKITNW
LKKSDSASVSVTKK---

---LADGILQRTAIGEEPFTVASDKGFSITTFKTLHMELSKSGQSIGFKKDDEGQSLDGE
KLAFTPKFSIPSSVLSKNKLKQTSEITGKLITVPSNIHFNHKSDQTIFPKTVSLELFNGG
SQLSINGLKDQIIIDMGKHSPPKDKEFKCGWWSSNKKEWLTDGCSTHKVDGNIQCKCNHL
TEFGLIEFAKSSQNIWIIIGPVVGGVVFAAAMVGTVVLVKRKKNKNKKNKSKKTKELINK
EKEDKKKQAKEKKEAXKWKKK
Translated Amino Acid sequence (All Frames) 
Frame A:
llhvhsvqtmvislvvisllqlilx*afm*fsflfcnftnfg*fskm*iycfkhsngyig
*w*kciswptigifsrwnhfkrw**e*lsnynigtttrfnssgry*sskcfrfm*sigir
c*sfkrw*wksinl*lvg*wgrykhsik*sigsk*cnhnnsk*sfviwsnlyfyt*nhkl
vkei**rkcfsnke---

---LADGILQRTAIGEEPFTVASDKGFSITTFKTLHMELSKSGQSIGFKKDDEGQSLDGE
KLAFTPKFSIPSSVLSKNKLKQTSEITGKLITVPSNIHFNHKSDQTIFPKTVSLELFNGG
SQLSINGLKDQIIIDMGKHSPPKDKEFKCGWWSSNKKEWLTDGCSTHKVDGNIQCKCNHL
TEFGLIEFAKSSQNIWIIIGPVVGGVVFAAAMVGTVVLVKRKKNKNKKNKSKKTKELINK
EKEDKKKQAKEKKEAXKWKKK

Frame B:
yfmctrckqw*fl*l*ylfss*YXNRPSCSSLFSSATLQTLGSSPRCEYTASNILMVTLG
SGESVSVGQQLEFSQGGITSKDGSKNNYQTITLEPPQDSTPVVDIKAPNVLDSCSPLVLD
ASHSKGGNGKALTYDWSVSGADTNTQLNNLLDQNNATITIPNDLLSSGQTYTFTLKITNW
LKKSDSASVSVTKK---

---wqmvffkelqlvknhlqlqvikvsrlqhlklyiwn*vrvvnrlvskrmmkvnllmvk
nllslqnsvfhqvfyqrin*nkplkslvn*lqfhqifisiinlikpfsqklfhlnfsmva
lnyqsmalkiklsliwenihhqkiknlnvvggapikkng*qmvvqpik*mvifnvnvtil
qslv*lnlqnplktfgslldq*lvvlylqlqwlvllyw*rerktktkrtnqrkpkn*stk
kkktkrnkqkkrkkxingkr

Frame C:
tscalganngnffscdisspvdtxiglhvvlfsllqlyklwvvlqdvnillqtf*wlhwv
vvkvyqlannwnflkveslqkmvvriiikl*hwnhhkiqlqw*ilklqmf*ihvvhwy*m
lviqkvvmekh*pmigrlvgqiqtln*iiywikimqp*qfqmifchlvklillhlksqig
*rnliaqvfq*qr---

---grwysskncnw*rtiysck**rfldyni*nstygik*ewsidwfqkg**rsis*w*k
tcfhskiqysikcfike*iktnl*nhw*tnyssikysfqs*i*snhfpkncft*tfqwwl
siinqwp*rsnyh*ygktfttkr*ri*mwlvelq*krmvnrwlfnp*srw*ysm*m*psy
rvwfn*ickilskhldhywtsswwccicscngwyccigkekekqkqkeqikenqrtnqqr
krrqketskrkersx*meke
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHL489 (SHL489Q) /CSM/SH/SHL4-D/SHL489Q.Seq.d/                       1283   0.0
SHL119 (SHL119Q) /CSM/SH/SHL1-A/SHL119Q.Seq.d/                       1283   0.0
SHK593 (SHK593Q) /CSM/SH/SHK5-D/SHK593Q.Seq.d/                       1283   0.0
SHJ824 (SHJ824Q) /CSM/SH/SHJ8-A/SHJ824Q.Seq.d/                       1283   0.0
SHD762 (SHD762Q) /CSM/SH/SHD7-C/SHD762Q.Seq.d/                       1283   0.0
SHD253 (SHD253Q) /CSM/SH/SHD2-C/SHD253Q.Seq.d/                       1283   0.0
SHB414 (SHB414Q) /CSM/SH/SHB4-A/SHB414Q.Seq.d/                       1283   0.0
SFG748 (SFG748Q) /CSM/SF/SFG7-B/SFG748Q.Seq.d/                       1283   0.0
CHS207 (CHS207Q) /CSM/CH/CHS2-A/CHS207Q.Seq.d/                       1283   0.0
CHS192 (CHS192Q) /CSM/CH/CHS1-D/CHS192Q.Seq.d/                       1283   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

BX119904|BX119904.4 Human DNA sequence from clone RP11-232D9 on chromosome X.    36   3e-05  15
AP004170|AP004170.1 Mycoplasma penetrans HF-2 DNA, complete genome, section 1/5.    34   0.37   13
AC013774|AC013774.3 Homo sapiens clone RP11-10P1, WORKING DRAFT SEQUENCE, 11 unordered pieces.    48   0.38   1
AC103541|AC103541.6 Homo sapiens BAC clone RP11-818M13 from 4, complete sequence.    48   0.38   1
AC097518|AC097518.2 Homo sapiens BAC clone RP11-487E13 from 4, complete sequence.    48   0.38   1
AC024453|AC024453.2 Homo sapiens chromosome 4 clone RP11-2B17 map 4, WORKING DRAFT SEQUENCE, 51 unordered pieces.    48   0.38   1
U66528|U66528.1 Dictyostelium discoideum CigB (cigB) mRNA, partial cds.    38   0.53   3
AC099715|AC099715.9 Mus musculus chromosome 10, clone RP23-346B18, complete sequence.    38   0.60   8
AC149573|AC149573.6 Medicago truncatula clone mth2-119g4, WORKING DRAFT SEQUENCE, 38 unordered pieces.    42   0.67   8
AJ410884|AJ410884.1 Influenza A virus (swine/Cotes d'Armor/1515/99 (H1N1)) partial N1NA  gene for Neuraminidase, genomic RNA.    42   0.74   2
dna update 2004. 9.25
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

EF203419_1(EF203419|pid:none) Strongylocentrotus purpuratus rece...    54   9e-06
AK154648_1(AK154648|pid:none) Mus musculus NOD-derived CD11c +ve...    50   2e-04
(Q8R0T6) RecName: Full=Probable G-protein coupled receptor 97; F...    50   2e-04
AK037149_1(AK037149|pid:none) Mus musculus 6 days neonate skin c...    50   2e-04
AB041939_1(AB041939|pid:none) Homo sapiens KPG_006 mRNA for G-pr...    50   2e-04
AF216967_1(AF216967|pid:none) Homo sapiens putative vascular ind...    48   7e-04
(Q8K3V3) RecName: Full=G-protein coupled receptor 56; Flags: Pre...    47   0.002
AK295345_1(AK295345|pid:none) Homo sapiens cDNA FLJ55998 complet...    46   0.003
AF166382_1(AF166382|pid:none) Mus musculus serpentine receptor (...    45   0.004
AY181246_1(AY181246|pid:none) Homo sapiens G protein-coupled rec...    45   0.008
protein update 2009. 3.31
PSORT 

 psg: 0.90  gvh: 0.59  alm: 0.22  top: 1.17  tms: 0.07  mit: 0.46  mip: 0.05
 nuc: 0.25  erl: 0.00  erm: 1.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.03  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 1.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: cytoplasmic
	 20.0 %: nuclear
	 12.0 %: mitochondrial
	 12.0 %: vesicles of secretory system
	  8.0 %: Golgi
	  8.0 %: endoplasmic reticulum
	  4.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal
	  4.0 %: plasma membrane

>> prediction for CHE648 is cyt
5' end seq. ID CHE648F
5' end seq. 
>CHE648F.Seq
TTACTTCATGTGCACTCGGTGCAAACAATGGTAATTTCTTTAGTTGTGATATCTCTTCTC
CAGTTGATACTTNAATAGGCCTTCATGTAGTTCTCTTTTCTCTTCTGCAACTTTACAAAC
TTTGGGTAGTTCTCCAAGATGTGAATATACTGCTTCAAACATTCTAATGGTTACATTGGG
TAGTGGTGAAAGTGTATCAGTTGGCCAACAATTGGAATTTTCTCAAGGTGGAATCACTTC
AAAAGATGGTAGTAAGAATAATTATCAAACTATAACATTGGAACCACCACAAGATTCAAC
TCCAGTGGTAGATATTAAAGCTCCAAATGTTTTAGATTCATGTAGTCCATTGGTATTAGA
TGCTAGTCATTCAAAAGGTGGTAATGGAAAAGCATTAACCTATGATTGGTCGGTTAGTGG
GGCAGATACAAACACTCAATTAAATAATCTATTGGATCAAAATAATGCAACCATAACAAT
TCCAAATGATCTTTTGTCATCTGGTCAAACTTATACTTTTACACTTAAAATCACAAATTG
GTTAAAGAAATCTGATAGCGCAAGTGTTTCAGTAACAAAGAAANNNNNNNNNN
Length of 5' end seq. 593
3' end seq. ID CHE648Z
3' end seq. 
>CHE648Z.Seq
NNNNNNNNNNTTGGCAGATGGTATTCTTCAAAGAACTGCAATTGGTGAAGAACCATTTAC
AGTTGCAAGTGATAAAGGTTTCTCGATTACAACATTTAAAACTCTACATATGGAATTAAG
TAAGAGTGGTCAATCGATTGGTTTCAAAAAGGATGATGAAGGTCAATCTCTTGATGGTGA
AAAACTTGCTTTCACTCCAAAATTCAGTATTCCATCAAGTGTTTTATCAAAGAATAAATT
AAAACAAACCTCTGAAATCACTGGTAAACTAATTACAGTTCCATCAAATATTCATTTCAA
TCATAAATCTGATCAAACCATTTTCCCAAAAACTGTTTCACTTGAACTTTTCAATGGTGG
CTCTCAATTATCAATCAATGGCCTTAAAGATCAAATTATCATTGATATGGGAAAACATTC
ACCACCAAAAGATAAAGAATTTAAATGTGGTTGGTGGAGCTCCAATAAAAAAGAATGGTT
AACAGATGGTTGTTCAACCCATAAAGTAGATGGTAATATTCAATGTAAATGTAACCATCT
TACAGAGTTTGGTTTAATTGAATTTGCAAAATCCTCTCAAAACATTTGGATCATTATTGG
ACCAGTAGTTGGTGGTGTTGTATTTGCAGCTGCAATGGTTGGTACTGTTGTATTGGTAAA
GAGAAAGAAAAACAAAAACAAAAAGAACAAATCAAAGAAAACCAAAGAACTAATCAACAA
AGAAAAAGAAGACAAAAAGAAACAAGCAAAAGAAAAGAAAGAAGCNNATAAATGGAAAAA
GAAA
Length of 3' end seq. 784
Connected seq. ID CHE648P
Connected seq. 
>CHE648P.Seq
TTACTTCATGTGCACTCGGTGCAAACAATGGTAATTTCTTTAGTTGTGATATCTCTTCTC
CAGTTGATACTTNAATAGGCCTTCATGTAGTTCTCTTTTCTCTTCTGCAACTTTACAAAC
TTTGGGTAGTTCTCCAAGATGTGAATATACTGCTTCAAACATTCTAATGGTTACATTGGG
TAGTGGTGAAAGTGTATCAGTTGGCCAACAATTGGAATTTTCTCAAGGTGGAATCACTTC
AAAAGATGGTAGTAAGAATAATTATCAAACTATAACATTGGAACCACCACAAGATTCAAC
TCCAGTGGTAGATATTAAAGCTCCAAATGTTTTAGATTCATGTAGTCCATTGGTATTAGA
TGCTAGTCATTCAAAAGGTGGTAATGGAAAAGCATTAACCTATGATTGGTCGGTTAGTGG
GGCAGATACAAACACTCAATTAAATAATCTATTGGATCAAAATAATGCAACCATAACAAT
TCCAAATGATCTTTTGTCATCTGGTCAAACTTATACTTTTACACTTAAAATCACAAATTG
GTTAAAGAAATCTGATAGCGCAAGTGTTTCAGTAACAAAGAAA----------TTGGCAG
ATGGTATTCTTCAAAGAACTGCAATTGGTGAAGAACCATTTACAGTTGCAAGTGATAAAG
GTTTCTCGATTACAACATTTAAAACTCTACATATGGAATTAAGTAAGAGTGGTCAATCGA
TTGGTTTCAAAAAGGATGATGAAGGTCAATCTCTTGATGGTGAAAAACTTGCTTTCACTC
CAAAATTCAGTATTCCATCAAGTGTTTTATCAAAGAATAAATTAAAACAAACCTCTGAAA
TCACTGGTAAACTAATTACAGTTCCATCAAATATTCATTTCAATCATAAATCTGATCAAA
CCATTTTCCCAAAAACTGTTTCACTTGAACTTTTCAATGGTGGCTCTCAATTATCAATCA
ATGGCCTTAAAGATCAAATTATCATTGATATGGGAAAACATTCACCACCAAAAGATAAAG
AATTTAAATGTGGTTGGTGGAGCTCCAATAAAAAAGAATGGTTAACAGATGGTTGTTCAA
CCCATAAAGTAGATGGTAATATTCAATGTAAATGTAACCATCTTACAGAGTTTGGTTTAA
TTGAATTTGCAAAATCCTCTCAAAACATTTGGATCATTATTGGACCAGTAGTTGGTGGTG
TTGTATTTGCAGCTGCAATGGTTGGTACTGTTGTATTGGTAAAGAGAAAGAAAAACAAAA
ACAAAAAGAACAAATCAAAGAAAACCAAAGAACTAATCAACAAAGAAAAAGAAGACAAAA
AGAAACAAGCAAAAGAAAAGAAAGAAGCNNATAAATGGAAAAAGAAA
Length of connected seq. 1357
Full length Seq ID -
Full length Seq. -
Length of full length seq. -