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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CHE561
Library CH
(Link to library)
Clone ID CHE561
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16419-1
Original site URL
Representative seq. ID CHE561P
(Link to Original site)
Representative DNA sequence 
>CHE561 (CHE561Q) /CSM/CH/CHE5-C/CHE561Q.Seq.d/
ATTCAATTTTATTTTTAATCATTCAAATTAAAAAAAAAACAATAACTAACTAAGTTAAAA
ATAAAAAAATAATAAAGGATGTTGTTTCTAAAAAATATTGGAGTATTTTTTATGATATTC
CTTGTATCAAAATCCTATGCGACGGATTGTAATAAAATTACTAATGAAGAANAATGTCAT
AAATCTTCGGAATGTATTGTTATAAATTATACACCATGTTGTGGTGAACAAAAATGGGCT
TGCTCTAAAGGTACATTTGATACTTGTACATATGAAAATAGTTGTTATANAAATTCATCC
AATAACCAAGTTGTTGAAGTATCAAATAAATGCTTCAATCTTGATGGATTTATTAAAATT
ACAACTCCAACCGAGTATTCTTGTTCTGATGCCAANATTAAAGAATGTGCCTTATTAGGT
AAATCATGTAGTTTCCAAAANAACTCTTGTTCAAATCCAACTTCCTGTTGCCCAGGTGAA
TCAATTTGTGAAGGTTTAAGCTCTGGTAGTTCAACATCTGGTGGTGGTTCATCAGGTGGT
ACATCAGGTGGTAGTTCATCAGGTGGTACATCAGGTGGTAGTTCATCAGGTGGTACATCA
GXXXXXXXXXXGTTTGTTNTTTAAGATGTCCACCCAANTCATGAATGTAGATTTAATGAC
CAAGGTCATCAATGCTGTGTAAAGGTTCACCATGATAGATGTTCATTAAGATGTCCACAT
GGCCCATGAATGTAAAGTTGATCACAATGGTAAAGAATGTTGCGTCAGATCCCATAGACC
ACCACCACCAGAAGTTTGTTCATTAAGATGCCCACCAAAACATGAATGTAAATTTGATGA
TCATGGTAAAAAATGTTGTGTAAAGATTCATTGTGGTGAAGTTTGTGATTTAGATTGTGG
TAGAGGTTTTGAATGTAAAATTAGACATGATGGTTCAAAATGTTGTGTCCGTTCAGAAAG
ACCACACCCACCACAACATGAAAAATGTAATAAGAGATGCCCACCAGGCCATGAATGTAA
AGTTGATCAACATGGAAAAGAATGTTGCGTTGTTGCCCATAGACCACCACCAAAATGCTC
TTTAAGATGTCCACCAAGACATGAATGTAGAGTCAACCACTTTGGTGAAGAATGTTGTGT
TAAAGTTCACCACGATAAATGTTCATTGAGATGTCCACCAGGCCATGAATGTAAAGTTGA
TCAACATGGAAAAGAATGTTGCGTTGTTGCCCATAGACCACCACCAAAATGNTCATTAAG
ATGTCCACCAAAACATGAATGTAGAATCAATCACTTTGGTGAAGAATGCTGTGTTAAAAG
TAGAAATGATTGTTTAACTTGTGAAGACCTAAACTGTGAAAGAAAAGGTTTACATTGTGC
CATGAAAACTGTACCAATTGATAAAGAAAATTGTTGTGAAAAAG
sequence update 2002.10.25
Translated Amino Acid sequence 
iqfyf*sfklkkkq*ltklkikk**RMLFLKNIGVFFMIFLVSKSYATDCNKITNEEXCH
KSSECIVINYTPCCGEQKWACSKGTFDTCTYENSCYXNSSNNQVVEVSNKCFNLDGFIKI
TTPTEYSCSDAXIKECALLGKSCSFQXNSCSNPTSCCPGESICEGLSSGSSTSGGGSSGG
TSGGSSSGGTSGGSSSGGTS---

---LXFKMSTQXMNVDLMTKVINAV*rftmidvh*dvhmaheckvdhngkeccvrshrpp
ppevcslrcppkheckfddhgkkccvkihcgevcdldcgrgfeckirhdgskccvrserp
hppqhekcnkrcppgheckvdqhgkeccvvahrpppkcslrcpprhecrvnhfgeeccvk
vhhdkcslrcppgheckvdqhgkeccvvahrpppkxslrcppkhecrinhfgeeccvksr
ndcltcedlncerkglhcamktvpidkenccek
Translated Amino Acid sequence (All Frames) 
Frame A:
iqfyf*sfklkkkq*ltklkikk**RMLFLKNIGVFFMIFLVSKSYATDCNKITNEEXCH
KSSECIVINYTPCCGEQKWACSKGTFDTCTYENSCYXNSSNNQVVEVSNKCFNLDGFIKI
TTPTEYSCSDAXIKECALLGKSCSFQXNSCSNPTSCCPGESICEGLSSGSSTSGGGSSGG
TSGGSSSGGTSGGSSSGGTS---

---vcxlrcppxs*m*i**prssmlckgsp**mfikmstwpmnvklitmvknvasdpidh
hhqkfvh*dahqnmnvnlmimvknvv*rfivvkfvi*ivvevlnvkldmmvqnvvsvqkd
hthhnmknvirdahqamnvklinmeknvallpidhhqnal*dvhqdmnvesttlvknvvl
kfttinvh*dvhqamnvklinmeknvallpidhhqnxh*dvhqnmnvesitlvknavlkv
emiv*lvkt*tvkekvyivp*klyqlikkivvkk

Frame B:
fnfifnhsn*kknnn*ls*k*knnkgccf*kileyfl*yslyqnpmrrivikllmkxnvi
nlrnvll*iihhvvvnknglalkvhlilvhmkivvixihpitkllkyqinasilmdllkl
qlqpsilvlmpxlknvpy*vnhvvskxtlvqiqlpvaqvnqfvkv*alvvqhlvvvhqvv
hqvvvhqvvhqvvvhqvvhq---

---fvx*dvhpxhecrfndqghqccvkvhhdrcslrcphgp*m*s*sqw*rmlrqip*tt
ttrslfikmptkt*m*i**sw*kmlckdslw*sl*frlw*rf*m*n*t*wfkmlcpfrkt
tpttt*km**emptrp*m*s*stwkrmlrccp*tttkmlfkmstkt*m*sqplw*rmlc*
sspr*mfiemstrp*m*s*stwkrmlrccp*tttkmxikmstkt*m*nqslw*rmlc*k*
k*lfnl*rpkl*kkrftlchenctn**rkll*k

Frame C:
silfliiqikkktitn*vknkkiikdvvskkywsifydipcikilcdgl**ny**rxms*
ifgmycyklytmlw*tkmgll*ryi*ylyi*k*llxkfiq*psc*sik*mlqs*wiy*ny
nsnrvflf*cqx*rmclir*im*fpkxllfksnfllpr*inl*rfklw*fniwwwfirwy
irw*firwyirw*firwyi---

---LXFKMSTQXMNVDLMTKVINAV*rftmidvh*dvhmaheckvdhngkeccvrshrpp
ppevcslrcppkheckfddhgkkccvkihcgevcdldcgrgfeckirhdgskccvrserp
hppqhekcnkrcppgheckvdqhgkeccvvahrpppkcslrcpprhecrvnhfgeeccvk
vhhdkcslrcppgheckvdqhgkeccvvahrpppkxslrcppkhecrinhfgeeccvksr
ndcltcedlncerkglhcamktvpidkenccek
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHE561 (CHE561Q) /CSM/CH/CHE5-C/CHE561Q.Seq.d/                       2498   0.0
CHH323 (CHH323Q) /CSM/CH/CHH3-A/CHH323Q.Seq.d/                       1564   0.0
CHL174 (CHL174Q) /CSM/CH/CHL1-D/CHL174Q.Seq.d/                       1562   0.0
CHQ549 (CHQ549Q) /CSM/CH/CHQ5-C/CHQ549Q.Seq.d/                       1560   0.0
SFI482 (SFI482Q) /CSM/SF/SFI4-D/SFI482Q.Seq.d/                       1558   0.0
CHI435 (CHI435Q) /CSM/CH/CHI4-B/CHI435Q.Seq.d/                       1554   0.0
AHK724 (AHK724Q) /CSM/AH/AHK7-A/AHK724Q.Seq.d/                       1554   0.0
AHA296 (AHA296Q) /CSM/AH/AHA2-D/AHA296Q.Seq.d/                       1554   0.0
CHL801 (CHL801Q) /CSM/CH/CHL8-A/CHL801Q.Seq.d/                       1552   0.0
AHD664 (AHD664Q) /CSM/AH/AHD6-C/AHD664Q.Seq.d/                       1552   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U25144|U25144.1 Dictyostelium discoideum spore coat protein SP87 (PspD) gene, complete cds.  1376   0.0    11
AC117267|AC117267.2 Dictyostelium discoideum chromosome 2 map 5836255-5862024 strain AX4, complete sequence.  1376   0.0    12
M26239|M26239.1 D.discoideum spore coat protein SP60 gene, complete cds.    66   1e-14  4
X51892|X51892.1 Dictyostelium discoideum SP60 gene for spore coat protein.    66   3e-14  4
CN574888|CN574888.1 rc36e04.x1 Meloidogyne hapla egg pAMP1 v1 Meloidogyne hapla cDNA 3', mRNA sequence.    42   0.007  2
AC124167|AC124167.2 Tetraodon nigroviridis clone GSTNB-42J2, WORKING DRAFT SEQUENCE, 14 unordered pieces.    38   0.013  5
AC150239|AC150239.2 Sorex araneus clone SA_Ba-527L4, WORKING DRAFT SEQUENCE, 7 ordered pieces.    36   0.022  8
X52105|X52105.1 Dictyostelium discoideum SP60 gene for spore coat protein.    48   0.042  3
AL139079|AL139079.2 Campylobacter jejuni NCTC11168 complete genome; segment 6/6.    42   0.11   7
AC141955|AC141955.2 Rattus norvegicus clone CH230-418P10, WORKING DRAFT SEQUENCE, 29 unordered pieces.    48   0.15   4
dna update 2004. 9.25
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC117267_7(AC117267|pid:none) Dictyostelium discoideum chromosom...   550   e-171
(P15270) RecName: Full=Spore coat protein SP60; Flags: Precursor...   186   2e-45
X52105_1(X52105|pid:none) Dictyostelium discoideum SP60 gene for...   110   9e-23
(Q6TU45) RecName: Full=Probable spore coat protein sigD; AltName...    94   1e-17
(Q54QX2) RecName: Full=Probable spore coat protein DDB_G0283555;...    82   3e-14
(Q04503) RecName: Full=Prespore protein Dp87; Flags: Precursor;        71   8e-11
S07638(S07638;A60942;B60942) spore coat protein SP96 precursor -...    70   2e-10
(P14328) RecName: Full=Spore coat protein SP96;  &AC117075_49(AC...    70   2e-10
D13973_1(D13973|pid:none) Dictyostelium discoideum gene for Dp87...    67   1e-09
(P15269) RecName: Full=Spore coat protein SP70; AltName: Full=Pr...    65   6e-09
protein update 2009. 3.31
PSORT 

 psg: 1.02  gvh: 0.68  alm: 0.51  top: 0.27  tms: 0.00  mit: 0.38  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 60.0 %: extracellular, including cell wall
	 12.0 %: cytoplasmic
	  8.0 %: mitochondrial
	  8.0 %: nuclear
	  8.0 %: endoplasmic reticulum
	  4.0 %: vacuolar

>> prediction for CHE561 is exc
5' end seq. ID CHE561F
5' end seq. 
>CHE561F.Seq
ATTCAATTTTATTTTTAATCATTCAAATTAAAAAAAAAACAATAACTAACTAAGTTAAAA
ATAAAAAAATAATAAAGGATGTTGTTTCTAAAAAATATTGGAGTATTTTTTATGATATTC
CTTGTATCAAAATCCTATGCGACGGATTGTAATAAAATTACTAATGAAGAANAATGTCAT
AAATCTTCGGAATGTATTGTTATAAATTATACACCATGTTGTGGTGAACAAAAATGGGCT
TGCTCTAAAGGTACATTTGATACTTGTACATATGAAAATAGTTGTTATANAAATTCATCC
AATAACCAAGTTGTTGAAGTATCAAATAAATGCTTCAATCTTGATGGATTTATTAAAATT
ACAACTCCAACCGAGTATTCTTGTTCTGATGCCAANATTAAAGAATGTGCCTTATTAGGT
AAATCATGTAGTTTCCAAAANAACTCTTGTTCAAATCCAACTTCCTGTTGCCCAGGTGAA
TCAATTTGTGAAGGTTTAAGCTCTGGTAGTTCAACATCTGGTGGTGGTTCATCAGGTGGT
ACATCAGGTGGTAGTTCATCAGGTGGTACATCAGGTGGTAGTTCATCAGGTGGTACATCA
GNNNNNNNNNN
Length of 5' end seq. 611
3' end seq. ID CHE561Z
3' end seq. 
>CHE561Z.Seq
NNNNNNNNNNGTTTGTTNTTTAAGATGTCCACCCAANTCATGAATGTAGATTTAATGACC
AAGGTCATCAATGCTGTGTAAAGGTTCACCATGATAGATGTTCATTAAGATGTCCACATG
GCCCATGAATGTAAAGTTGATCACAATGGTAAAGAATGTTGCGTCAGATCCCATAGACCA
CCACCACCAGAAGTTTGTTCATTAAGATGCCCACCAAAACATGAATGTAAATTTGATGAT
CATGGTAAAAAATGTTGTGTAAAGATTCATTGTGGTGAAGTTTGTGATTTAGATTGTGGT
AGAGGTTTTGAATGTAAAATTAGACATGATGGTTCAAAATGTTGTGTCCGTTCAGAAAGA
CCACACCCACCACAACATGAAAAATGTAATAAGAGATGCCCACCAGGCCATGAATGTAAA
GTTGATCAACATGGAAAAGAATGTTGCGTTGTTGCCCATAGACCACCACCAAAATGCTCT
TTAAGATGTCCACCAAGACATGAATGTAGAGTCAACCACTTTGGTGAAGAATGTTGTGTT
AAAGTTCACCACGATAAATGTTCATTGAGATGTCCACCAGGCCATGAATGTAAAGTTGAT
CAACATGGAAAAGAATGTTGCGTTGTTGCCCATAGACCACCACCAAAATGNTCATTAAGA
TGTCCACCAAAACATGAATGTAGAATCAATCACTTTGGTGAAGAATGCTGTGTTAAAAGT
AGAAATGATTGTTTAACTTGTGAAGACCTAAACTGTGAAAGAAAAGGTTTACATTGTGCC
ATGAAAACTGTACCAATTGATAAAGAAAATTGTTGTGAAAAAG
Length of 3' end seq. 823
Connected seq. ID CHE561P
Connected seq. 
>CHE561P.Seq
ATTCAATTTTATTTTTAATCATTCAAATTAAAAAAAAAACAATAACTAACTAAGTTAAAA
ATAAAAAAATAATAAAGGATGTTGTTTCTAAAAAATATTGGAGTATTTTTTATGATATTC
CTTGTATCAAAATCCTATGCGACGGATTGTAATAAAATTACTAATGAAGAANAATGTCAT
AAATCTTCGGAATGTATTGTTATAAATTATACACCATGTTGTGGTGAACAAAAATGGGCT
TGCTCTAAAGGTACATTTGATACTTGTACATATGAAAATAGTTGTTATANAAATTCATCC
AATAACCAAGTTGTTGAAGTATCAAATAAATGCTTCAATCTTGATGGATTTATTAAAATT
ACAACTCCAACCGAGTATTCTTGTTCTGATGCCAANATTAAAGAATGTGCCTTATTAGGT
AAATCATGTAGTTTCCAAAANAACTCTTGTTCAAATCCAACTTCCTGTTGCCCAGGTGAA
TCAATTTGTGAAGGTTTAAGCTCTGGTAGTTCAACATCTGGTGGTGGTTCATCAGGTGGT
ACATCAGGTGGTAGTTCATCAGGTGGTACATCAGGTGGTAGTTCATCAGGTGGTACATCA
G----------GTTTGTTNTTTAAGATGTCCACCCAANTCATGAATGTAGATTTAATGAC
CAAGGTCATCAATGCTGTGTAAAGGTTCACCATGATAGATGTTCATTAAGATGTCCACAT
GGCCCATGAATGTAAAGTTGATCACAATGGTAAAGAATGTTGCGTCAGATCCCATAGACC
ACCACCACCAGAAGTTTGTTCATTAAGATGCCCACCAAAACATGAATGTAAATTTGATGA
TCATGGTAAAAAATGTTGTGTAAAGATTCATTGTGGTGAAGTTTGTGATTTAGATTGTGG
TAGAGGTTTTGAATGTAAAATTAGACATGATGGTTCAAAATGTTGTGTCCGTTCAGAAAG
ACCACACCCACCACAACATGAAAAATGTAATAAGAGATGCCCACCAGGCCATGAATGTAA
AGTTGATCAACATGGAAAAGAATGTTGCGTTGTTGCCCATAGACCACCACCAAAATGCTC
TTTAAGATGTCCACCAAGACATGAATGTAGAGTCAACCACTTTGGTGAAGAATGTTGTGT
TAAAGTTCACCACGATAAATGTTCATTGAGATGTCCACCAGGCCATGAATGTAAAGTTGA
TCAACATGGAAAAGAATGTTGCGTTGTTGCCCATAGACCACCACCAAAATGNTCATTAAG
ATGTCCACCAAAACATGAATGTAGAATCAATCACTTTGGTGAAGAATGCTGTGTTAAAAG
TAGAAATGATTGTTTAACTTGTGAAGACCTAAACTGTGAAAGAAAAGGTTTACATTGTGC
CATGAAAACTGTACCAATTGATAAAGAAAATTGTTGTGAAAAAG
Length of connected seq. 1414
Full length Seq ID -
Full length Seq. -
Length of full length seq. -