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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CHE482
Library CH
(Link to library)
Clone ID CHE482
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15645-1
Original site URL
Representative seq. ID CHE482P
(Link to Original site)
Representative DNA sequence 
>CHE482 (CHE482Q) /CSM/CH/CHE4-D/CHE482Q.Seq.d/
CTGTTGGCCTACTGGATTTTNCTTTTACTTAAATAATTTCCAAAATGTCAGAAACTACAC
CAGTTCCTGTTGGCGAANCAGGTGAAAAAAGTGCTACAGCATTAAAAAAAGAACAAAAGA
AATTAGAAAAAGAGAAAAAGATAGCAGAAGCTAAAGCAAAGAAAGCCGCTGAGAAATCAG
CAGCAAGTGGTAATAGTGGTGAACAAAAGAATGAAAAGAAACAAGAACAAGTTGAAGAGA
ATAATAGTAATTGGGGTGAATTACCAATGAATCAAAGTAAAGAAAAGATTACAAGAGAAT
GGACAGATGTATCACAATTGACAGAATCATTAGTTGGTAAATCAGTATTAATTCGNGCAC
GTNTTTCAACAAGTCGTTTACAAGGTGCCAATCTTTGTTXXXXXXXXXXCTTTAAGGATC
ATTATCATGAGGCTTTGGATACTTTGGATCGTTTAATGACCTCGATTTTCCGTGGTTTAG
AGACTCGTTTCGCAAAGGAAATCGAATCTGTAAACACTCAATACCCATTCGAACCTTTCA
AATTCACTTATCCATCACCACGTTTCACCTTTGATGAAGCCGCTGCTATGTTGGCCGAAT
TAAATGACCCAGATTACATTGTAAAAGACAATGATTTCAATACTCGTCAANAGAAACGTT
TAGGTAAAATCATCAAAGAGAAATTTGGTGTAGATTTCTTTATCGTCGATAAATTCCACG
TTGAAGTTCGTCCATTNTATACCATGCCTGATCCAAATAATCCNCAATGGGCAAATGCTT
ACGATCTCTTTATGANAGGTGAAGAAATTTGTTCTGGTGCTCAACGTATTCATGATCCAG
AATTACTCGAAAAGAGTGCTAAATCTCATGGTGTTGTTATTGAGGATATTCAAGGTTANA
TCGATTCCTTTAAATATGGTTGTTCACAACATGCTGGTGGTGGTGTTGGTTTAGAACGTG
TAGTTATGCTTTATTTAGGTTTAGGTAATATTAGAAAAGCTAGTTTCTGTCCACGT
sequence update 2002.10.25
Translated Amino Acid sequence 
vglldfxft*IISKMSETTPVPVGEXGEKSATALKKEQKKLEKEKKIAEAKAKKAAEKSA
ASGNSGEQKNEKKQEQVEENNSNWGELPMNQSKEKITREWTDVSQLTESLVGKSVLIRAR
XSTSRLQGANLC---

---FKDHYHEALDTLDRLMTSIFRGLETRFAKEIESVNTQYPFEPFKFTYPSPRFTFDEA
AAMLAELNDPDYIVKDNDFNTRQXKRLGKIIKEKFGVDFFIVDKFHVEVRPXYTMPDPNN
PQWANAYDLFMXGEEICSGAQRIHDPELLEKSAKSHGVVIEDIQGXIDSFKYGCSQHAGG
GVGLERVVMLYLGLGNIRKASFCPR
Translated Amino Acid sequence (All Frames) 
Frame A:
llaywixlllk*fpkcqklhqfllaxqvkkvlqh*kknkrn*kkrkr*qklkqrkplrnq
qqvvivvnkrmkrnknklkriivigvnyq*ikvkkrlqengqmyhn*qnh*lvnqy*fxh
vfqqvvykvpifv---

---l*gsls*gfgyfgsfndldfpwfrdsfrkgnrickhsipirtfqihlsittfhl**s
rcyvgrik*prlhckrq*fqyssxetfr*nhqreiwcrflyrr*ipr*sssixyha*sk*
sxmgkclrslyxr*rnlfwcstys*sritrkec*iswccy*gysrlxrfl*iwlfttcww
wcwfrtcsyalfrfr*y*ks*flst

Frame B:
cwptgfxfylnnfqnvrnytsscwrxr*kkcysikkrtkeirkrekdsrs*skesr*eis
skw**w*tke*ketrts*re***lg*itnesk*rkdykrmdrcitidriisw*isinsxt
xfnksftrcqsl---

---FKDHYHEALDTLDRLMTSIFRGLETRFAKEIESVNTQYPFEPFKFTYPSPRFTFDEA
AAMLAELNDPDYIVKDNDFNTRQXKRLGKIIKEKFGVDFFIVDKFHVEVRPXYTMPDPNN
PQWANAYDLFMXGEEICSGAQRIHDPELLEKSAKSHGVVIEDIQGXIDSFKYGCSQHAGG
GVGLERVVMLYLGLGNIRKASFCPR

Frame C:
vglldfxft*IISKMSETTPVPVGEXGEKSATALKKEQKKLEKEKKIAEAKAKKAAEKSA
ASGNSGEQKNEKKQEQVEENNSNWGELPMNQSKEKITREWTDVSQLTESLVGKSVLIRAR
XSTSRLQGANLC---

---lriiimrlwilwiv**prfsvv*rlvsqrksnl*tlnthsnlsnslihhhvsplmkp
llcwpn*mtqitl*ktmisilvxrnv*vksskrnlv*islssinstlkfvhxipcliqii
xngqmltisl*xvkkfvlvlnvfmiqnyskrvlnlmvlllrifkvxsiplnmvvhnmlvv
vlv*nv*lcfi*v*vileklvsvh
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHE482 (CHE482Q) /CSM/CH/CHE4-D/CHE482Q.Seq.d/                       1637   0.0
VFK794 (VFK794Q) /CSM/VF/VFK7-D/VFK794Q.Seq.d/                       1174   0.0
CFJ701 (CFJ701Q) /CSM/CF/CFJ7-A/CFJ701Q.Seq.d/                       1174   0.0
VHK208 (VHK208Q) /CSM/VH/VHK2-A/VHK208Q.Seq.d/                       1172   0.0
VFH716 (VFH716Q) /CSM/VF/VFH7-A/VFH716Q.Seq.d/                       1172   0.0
VFB754 (VFB754Q) /CSM/VF/VFB7-C/VFB754Q.Seq.d/                       1172   0.0
AFN242 (AFN242Q) /CSM/AF/AFN2-B/AFN242Q.Seq.d/                       1172   0.0
AFM449 (AFM449Q) /CSM/AF/AFM4-C/AFM449Q.Seq.d/                       1172   0.0
AFK839 (AFK839Q) /CSM/AF/AFK8-B/AFK839Q.Seq.d/                       1172   0.0
AFK573 (AFK573Q) /CSM/AF/AFK5-D/AFK573Q.Seq.d/                       1172   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116963|AC116963.2 Dictyostelium discoideum chromosome 2 map 4657875-4914984 strain AX4, complete sequence.  1174   0.0    9
BG736765|BG736765.1 rk62b01.y1 Meloidogyne javanica egg pAMP1 v6 Chiapelli McCarter Meloidogyne javanica cDNA 5' similar to SW:SYD_CAEEL Q03577 PROBABLE ASPARTYL-TRNA SYNTHETASE ;, mRNA sequence.    58   8e-17  3
AX489090|AX489090.1 Sequence 6390 from Patent WO02053728.          84   2e-15  2
Y12709|Y12709.1 S.pombe tea1p gene.                                58   7e-10  2
AL031579|AL031579.1 S.pombe chromosome III cosmid c1223.           58   3e-09  5
CN555339|CN555339.1 tae15e05.x1 Hydra EST Darmstadt I Hydra magnipapillata cDNA 3' similar to TR:Q9XYM1 Q9XYM1 ASPARTYL TRNA SYNTHETASE. [1] ;, mRNA sequence.    44   9e-08  2
BI503282|BI503282.1 BB170009B20C08.5 Bee Brain Normalized/Subtracted Library, BB17 Apis mellifera cDNA clone BB170009B20C08 5', mRNA sequence.    52   9e-08  2
CR382124|CR382124.1 Kluyveromyces lactis strain NRRL Y-1140 chromosome D of strain NRRL  Y-1140 of Kluyveromyces lactis.    50   1e-06  2
AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.    34   3e-04  13
BI508409|BI508409.1 BB170009B21C08.5 Bee Brain Normalized/Subtracted Library, BB17 Apis mellifera cDNA clone BB170009B21C08 5', mRNA sequence.    40   3e-04  2
dna update 2004. 9.25
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q75JQ1) RecName: Full=Aspartyl-tRNA synthetase, cytoplasmic 1; ...   410   e-113
AJ720378_1(AJ720378|pid:none) Gallus gallus mRNA for hypothetica...   222   2e-56
AB171471_1(AB171471|pid:none) Macaca fascicularis brain cDNA clo...   219   1e-55
BC107749_1(BC107749|pid:none) Homo sapiens aspartyl-tRNA synthet...   219   1e-55
AK222476_1(AK222476|pid:none) Homo sapiens mRNA for aspartyl-tRN...   219   1e-55
(P14868) RecName: Full=Aspartyl-tRNA synthetase, cytoplasmic;   ...   219   1e-55
AY891847_1(AY891847|pid:none) Synthetic construct Homo sapiens c...   219   1e-55
AY762100_1(AY762100|pid:none) Homo sapiens cell proliferation-in...   218   2e-55
(P15178) RecName: Full=Aspartyl-tRNA synthetase, cytoplasmic;   ...   218   3e-55
AB171146_1(AB171146|pid:none) Macaca fascicularis brain cDNA clo...   217   4e-55
protein update 2009. 3.30
PSORT 

 psg: 0.75  gvh: 0.21  alm: 0.42  top: 0.53  tms: 0.00  mit: 0.24  mip: 0.00
 nuc: 0.06  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: nuclear
	 28.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: Golgi
	  4.0 %: plasma membrane

>> prediction for CHE482 is nuc
5' end seq. ID CHE482F
5' end seq. 
>CHE482F.Seq
CTGTTGGCCTACTGGATTTTNCTTTTACTTAAATAATTTCCAAAATGTCAGAAACTACAC
CAGTTCCTGTTGGCGAANCAGGTGAAAAAAGTGCTACAGCATTAAAAAAAGAACAAAAGA
AATTAGAAAAAGAGAAAAAGATAGCAGAAGCTAAAGCAAAGAAAGCCGCTGAGAAATCAG
CAGCAAGTGGTAATAGTGGTGAACAAAAGAATGAAAAGAAACAAGAACAAGTTGAAGAGA
ATAATAGTAATTGGGGTGAATTACCAATGAATCAAAGTAAAGAAAAGATTACAAGAGAAT
GGACAGATGTATCACAATTGACAGAATCATTAGTTGGTAAATCAGTATTAATTCGNGCAC
GTNTTTCAACAAGTCGTTTACAAGGTGCCAATCTTTGTTNNNNNNNNNN
Length of 5' end seq. 409
3' end seq. ID CHE482Z
3' end seq. 
>CHE482Z.Seq
NNNNNNNNNNCTTTAAGGATCATTATCATGAGGCTTTGGATACTTTGGATCGTTTAATGA
CCTCGATTTTCCGTGGTTTAGAGACTCGTTTCGCAAAGGAAATCGAATCTGTAAACACTC
AATACCCATTCGAACCTTTCAAATTCACTTATCCATCACCACGTTTCACCTTTGATGAAG
CCGCTGCTATGTTGGCCGAATTAAATGACCCAGATTACATTGTAAAAGACAATGATTTCA
ATACTCGTCAANAGAAACGTTTAGGTAAAATCATCAAAGAGAAATTTGGTGTAGATTTCT
TTATCGTCGATAAATTCCACGTTGAAGTTCGTCCATTNTATACCATGCCTGATCCAAATA
ATCCNCAATGGGCAAATGCTTACGATCTCTTTATGANAGGTGAAGAAATTTGTTCTGGTG
CTCAACGTATTCATGATCCAGAATTACTCGAAAAGAGTGCTAAATCTCATGGTGTTGTTA
TTGAGGATATTCAAGGTTANATCGATTCCTTTAAATATGGTTGTTCACAACATGCTGGTG
GTGGTGTTGGTTTAGAACGTGTAGTTATGCTTTATTTAGGTTTAGGTAATATTAGAAAAG
CTAGTTTCTGTCCACGT
Length of 3' end seq. 617
Connected seq. ID CHE482P
Connected seq. 
>CHE482P.Seq
CTGTTGGCCTACTGGATTTTNCTTTTACTTAAATAATTTCCAAAATGTCAGAAACTACAC
CAGTTCCTGTTGGCGAANCAGGTGAAAAAAGTGCTACAGCATTAAAAAAAGAACAAAAGA
AATTAGAAAAAGAGAAAAAGATAGCAGAAGCTAAAGCAAAGAAAGCCGCTGAGAAATCAG
CAGCAAGTGGTAATAGTGGTGAACAAAAGAATGAAAAGAAACAAGAACAAGTTGAAGAGA
ATAATAGTAATTGGGGTGAATTACCAATGAATCAAAGTAAAGAAAAGATTACAAGAGAAT
GGACAGATGTATCACAATTGACAGAATCATTAGTTGGTAAATCAGTATTAATTCGNGCAC
GTNTTTCAACAAGTCGTTTACAAGGTGCCAATCTTTGTT----------CTTTAAGGATC
ATTATCATGAGGCTTTGGATACTTTGGATCGTTTAATGACCTCGATTTTCCGTGGTTTAG
AGACTCGTTTCGCAAAGGAAATCGAATCTGTAAACACTCAATACCCATTCGAACCTTTCA
AATTCACTTATCCATCACCACGTTTCACCTTTGATGAAGCCGCTGCTATGTTGGCCGAAT
TAAATGACCCAGATTACATTGTAAAAGACAATGATTTCAATACTCGTCAANAGAAACGTT
TAGGTAAAATCATCAAAGAGAAATTTGGTGTAGATTTCTTTATCGTCGATAAATTCCACG
TTGAAGTTCGTCCATTNTATACCATGCCTGATCCAAATAATCCNCAATGGGCAAATGCTT
ACGATCTCTTTATGANAGGTGAAGAAATTTGTTCTGGTGCTCAACGTATTCATGATCCAG
AATTACTCGAAAAGAGTGCTAAATCTCATGGTGTTGTTATTGAGGATATTCAAGGTTANA
TCGATTCCTTTAAATATGGTTGTTCACAACATGCTGGTGGTGGTGTTGGTTTAGAACGTG
TAGTTATGCTTTATTTAGGTTTAGGTAATATTAGAAAAGCTAGTTTCTGTCCACGT
Length of connected seq. 1006
Full length Seq ID -
Full length Seq. -
Length of full length seq. -