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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CHE273
Library CH
(Link to library)
Clone ID CHE273
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U10863-1
Original site URL
Representative seq. ID CHE273P
(Link to Original site)
Representative DNA sequence 
>CHE273 (CHE273Q) /CSM/CH/CHE2-D/CHE273Q.Seq.d/
AGCATTTAATGCATCGATTGCACCAACACCACCAGCGCCNTTTTGAGTGAAAACATATTG
TGAAGCTCTTCTAGATGCACGAATTGCTTCACCACCTTTCATTTTACTCATTAATTGATC
CATAAAAGTACCATTTTGTGGTATATCATCATCGTCGTCGCCATGGTGTTGTTGTTGTTG
TTGCTGAACAGAAATTGATGGTTGAGTTGATTGTGGTGGTTTTTGTGAGATTGAAATTGA
AGATTTTGAAGTTGGAGTAATTGGAGAAACACCAGATTTTGGAGCGATATTAATTGAAGA
ACCAGAAGCAGATGGCACAGATGGACCAGAAGCAGCAGCTTTCTTTCTAGCGGCTAATTT
TTGAACATTTTCTCTTTCCTTTTGAATTGATTCAATTGTACGTTTGAATTCATTTTTGAA
TTTTGAAACATGTTGGAAGAATTCTTCAGGTGATGAAGTTTTAACATCTTCACCATAGAA
TTGTACGGTTGAATTAAAXXXXXXXXXXGNNNCCGTAAANATCACCACGTGGTGTNCCAC
CATTAATGAAATTACCCAATGATAATNCAATCTNTAGAATTTGATGGAATGAATTATTAT
TTTTGGTTTCCATTGAACCTTTTAATAATGCGTTAATATCTGGTGTTAATTCTTCGATTT
GAGTTGAAAGTTTTTGTTTAAAGATAAAACATTCCAATTTCTCAGAGATTCTATTGATTT
TACTAAGTNCCCAAAGGAATTGTTGCTGGTTTACCAAAACATGATGGATCCTGCTTTCTT
TTNTATAATNCAATTTCCNCTGAACNTG
sequence update 2002.10.25
Translated Amino Acid sequence 
afnasiaptppapf*vktycealldariasppfillin*sikvpfcgissssspwccccc
c*teidg*vdcggfceieiedfevgvigetpdfgailieepeadgtdgpeaaafflaanf
*tfslsf*idsivrlnsflnfetcwknssgdevltssp*NCTVEL---

---XXVXITTWCXTINEITQ**xnl*nlme*iiifgfh*tf**cvniwc*ffdls*kflf
kdktfqflrdsidftkxpkelllvyqnmmdpaffxixqfplnx
Translated Amino Acid sequence (All Frames) 
Frame A:
si*cidctnttsaxlsenil*sssrctncfttfhfth*lihkstilwyiiivvamvllll
llnrn*wls*lwwfl*d*n*rf*swsnwrntrfwsdin*rtrsrwhrwtrsssflssg*f
lnifsflln*fnctfefifef*nmleeffr**sfniftielyg*ik---

---XXVXITTWCXTINEITQ**xnl*nlme*iiifgfh*tf**cvniwc*ffdls*kflf
kdktfqflrdsidftkxpkelllvyqnmmdpaffxixqfplnx

Frame B:
afnasiaptppapf*vktycealldariasppfillin*sikvpfcgissssspwccccc
c*teidg*vdcggfceieiedfevgvigetpdfgailieepeadgtdgpeaaafflaanf
*tfslsf*idsivrlnsflnfetcwknssgdevltssp*NCTVEL---

---xp*xsprgvpplmklpndnxixri*wnellflvsiepfnnalisgvnssi*vesfcl
kikhsnfseillillsxqrnccwftkt*willsfx*xnfx*t

Frame C:
hlmhrlhqhhqrxfe*khivklf*mhellhhlsfyslidp*kyhfvvyhhrrrhgvvvvv
aeqklmvelivvvfvrlklkilkle*lekhqilery*lknqkqmaqmdqkqqlsf*rlif
ehflfpfeliqlyv*ihf*ilkhvgrilqvmkf*hlhhrivrln*---

---xrkxhhvvxhh**nypmixqsxefdgmnyyfwfplnllimr*ylvlilrfelkvfv*
r*nipisqrfy*fy*vpkgivaglpkhdgscflxynxisxex
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHE273 (CHE273Q) /CSM/CH/CHE2-D/CHE273Q.Seq.d/                       1108   0.0
VSF214 (VSF214Q) /CSM/VS/VSF2-A/VSF214Q.Seq.d/                        624   e-178
SHJ417 (SHJ417Q) /CSM/SH/SHJ4-A/SHJ417Q.Seq.d/                        624   e-178
SHI557 (SHI557Q) /CSM/SH/SHI5-C/SHI557Q.Seq.d/                        624   e-178
SFK280 (SFK280Q) /CSM/SF/SFK2-D/SFK280Q.Seq.d/                        624   e-178
CHD389 (CHD389Q) /CSM/CH/CHD3-D/CHD389Q.Seq.d/                        624   e-178
CHA791 (CHA791Q) /CSM/CH/CHA7-D/CHA791Q.Seq.d/                        624   e-178
AFF556 (AFF556Q) /CSM/AF/AFF5-C/AFF556Q.Seq.d/                        624   e-178
SLI478 (SLI478Q) /CSM/SL/SLI4-D/SLI478Q.Seq.d/                        361   1e-98
SFG768 (SFG768Q) /CSM/SF/SFG7-C/SFG768Q.Seq.d/                        311   9e-84
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AB082543|AB082543.1 Dictyostelium discoideum forB gene for formin homology protein B, complete cds.   624   0.0    4
AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.    30   3e-05  18
AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.    42   9e-05  16
AP003232|AP003232.3 Oryza sativa (japonica cultivar-group) genomic DNA, chromosome 1, PAC clone:P0034E02.    38   0.035  6
AC117075|AC117075.2 Dictyostelium discoideum chromosome 2 map 5201047-5455781 strain AX4, complete sequence.    30   0.086  13
AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.    30   0.10   18
AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.    32   0.13   15
AC006799|AC006799.1 Caenorhabditis elegans clone Y51H7, WORKING DRAFT SEQUENCE, 7 unordered pieces.    34   0.33   11
AC006880|AC006880.1 Caenorhabditis elegans clone Y55D5, WORKING DRAFT SEQUENCE, 4 unordered pieces.    36   0.36   11
AX602207|AX602207.1 Sequence 136 from Patent WO02092818.           38   0.52   4
dna update 2004. 9.21
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AB082543_1(AB082543|pid:none) Dictyostelium discoideum forB gene...   186   6e-46
(Q54SP2) RecName: Full=Formin-B;                                      186   6e-46
(Q54N00) RecName: Full=Formin-H; AltName: Full=Diaphanous-relate...    67   8e-10
BX324141_1(BX324141|pid:none) Zebrafish DNA sequence from clone ...    64   5e-09
BC163491_1(BC163491|pid:none) Danio rerio dishevelled associated...    64   5e-09
BC073482_1(BC073482|pid:none) Xenopus laevis hypothetical protei...    60   8e-08
(Q9Y4D1) RecName: Full=Disheveled-associated activator of morpho...    59   2e-07
AP008216_57(AP008216|pid:none) Oryza sativa (japonica cultivar-g...    59   2e-07
BC076585_1(BC076585|pid:none) Mus musculus dishevelled associate...    59   2e-07
BC133280_1(BC133280|pid:none) Bos taurus dishevelled associated ...    59   2e-07
protein update 2009. 3.30
PSORT 

 psg: 0.75  gvh: 0.48  alm: 0.45  top: 0.53  tms: 0.00  mit: 0.25  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: cytoplasmic
	 32.0 %: nuclear
	  8.0 %: cytoskeletal
	  8.0 %: mitochondrial
	  4.0 %: Golgi
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for CHE273 is cyt
5' end seq. ID CHE273F
5' end seq. 
>CHE273F.Seq
AGCATTTAATGCATCGATTGCACCAACACCACCAGCGCCNTTTTGAGTGAAAACATATTG
TGAAGCTCTTCTAGATGCACGAATTGCTTCACCACCTTTCATTTTACTCATTAATTGATC
CATAAAAGTACCATTTTGTGGTATATCATCATCGTCGTCGCCATGGTGTTGTTGTTGTTG
TTGCTGAACAGAAATTGATGGTTGAGTTGATTGTGGTGGTTTTTGTGAGATTGAAATTGA
AGATTTTGAAGTTGGAGTAATTGGAGAAACACCAGATTTTGGAGCGATATTAATTGAAGA
ACCAGAAGCAGATGGCACAGATGGACCAGAAGCAGCAGCTTTCTTTCTAGCGGCTAATTT
TTGAACATTTTCTCTTTCCTTTTGAATTGATTCAATTGTACGTTTGAATTCATTTTTGAA
TTTTGAAACATGTTGGAAGAATTCTTCAGGTGATGAAGTTTTAACATCTTCACCATAGAA
TTGTACGGTTGAATTAAANNNNNNNNNN
Length of 5' end seq. 508
3' end seq. ID CHE273Z
3' end seq. 
>CHE273Z.Seq
NNNNNNNNNNGNNNCCGTAAANATCACCACGTGGTGTNCCACCATTAATGAAATTACCCA
ATGATAATNCAATCTNTAGAATTTGATGGAATGAATTATTATTTTTGGTTTCCATTGAAC
CTTTTAATAATGCGTTAATATCTGGTGTTAATTCTTCGATTTGAGTTGAAAGTTTTTGTT
TAAAGATAAAACATTCCAATTTCTCAGAGATTCTATTGATTTTACTAAGTNCCCAAAGGA
ATTGTTGCTGGTTTACCAAAACATGATGGATCCTGCTTTCTTTTNTATAATNCAATTTCC
NCTGAACNTG
Length of 3' end seq. 310
Connected seq. ID CHE273P
Connected seq. 
>CHE273P.Seq
AGCATTTAATGCATCGATTGCACCAACACCACCAGCGCCNTTTTGAGTGAAAACATATTG
TGAAGCTCTTCTAGATGCACGAATTGCTTCACCACCTTTCATTTTACTCATTAATTGATC
CATAAAAGTACCATTTTGTGGTATATCATCATCGTCGTCGCCATGGTGTTGTTGTTGTTG
TTGCTGAACAGAAATTGATGGTTGAGTTGATTGTGGTGGTTTTTGTGAGATTGAAATTGA
AGATTTTGAAGTTGGAGTAATTGGAGAAACACCAGATTTTGGAGCGATATTAATTGAAGA
ACCAGAAGCAGATGGCACAGATGGACCAGAAGCAGCAGCTTTCTTTCTAGCGGCTAATTT
TTGAACATTTTCTCTTTCCTTTTGAATTGATTCAATTGTACGTTTGAATTCATTTTTGAA
TTTTGAAACATGTTGGAAGAATTCTTCAGGTGATGAAGTTTTAACATCTTCACCATAGAA
TTGTACGGTTGAATTAAA----------GNNNCCGTAAANATCACCACGTGGTGTNCCAC
CATTAATGAAATTACCCAATGATAATNCAATCTNTAGAATTTGATGGAATGAATTATTAT
TTTTGGTTTCCATTGAACCTTTTAATAATGCGTTAATATCTGGTGTTAATTCTTCGATTT
GAGTTGAAAGTTTTTGTTTAAAGATAAAACATTCCAATTTCTCAGAGATTCTATTGATTT
TACTAAGTNCCCAAAGGAATTGTTGCTGGTTTACCAAAACATGATGGATCCTGCTTTCTT
TTNTATAATNCAATTTCCNCTGAACNTG
Length of connected seq. 798
Full length Seq ID -
Full length Seq. -
Length of full length seq. -