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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CHE249
Library CH
(Link to library)
Clone ID CHE249
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U14926-1
Original site URL
Representative seq. ID CHE249P
(Link to Original site)
Representative DNA sequence 
>CHE249 (CHE249Q) /CSM/CH/CHE2-C/CHE249Q.Seq.d/
TGGCCTACTGGTTATTACAACTCAAACATCCACCATACACTTTTTATAGTATTACTACTT
TAAAATTTGACCTATTTTTTATTTATTAAATTAAATAATTCATTGTAAATTCGATAATAA
GGGGATTTAAAAAAGGAATCATTTTTTTTCACTCTTTATTTTGTACACTATAAAAAAAAA
AXXXXXXXXXXTGGTGTTGTNGTAGATTCAGGTGATGGTGTCACCCATATCATTNCAGTN
TATGAGGGTTANTCAATCCCACATTTGACTNGTNGTNTNGATGTTGCCGGTCGTGATGTA
ACTCGTTACCTCATCAAATTATTATTACTCCGTGGTTACGCTTTCAATCGTACCGCCGAT
TTNGAAACCATCCGTCAAATCAAAGAGAAACTCTGTTATGTCGCATACGATGTTCAACAA
GAGATGAAATTGGCCTCAGAGACCACTGTTCTCGTCGAAAACTACACCCTCCCAGACGGT
CGTGTAATCAAAGTTGGCCAAGAGAGATTCCAAGCCTCTGAAGCCCTCTTCAATCCATCT
TTGGTCGATGTCGAAGGTGGTGGTGTCCATGAACAATTGTTTGATTGTATCACCAAGGCA
GACCGTGATCTTCAACAAGGTTTCTATCAACATATCGTCCTTTCTGGTGGTTCCTCTATG
TATCCAGGTTTACC
sequence update 2002.10.25
Translated Amino Acid sequence 
wptgyynsnihhtlfivlll*nltyflfiklnnsl*ir**GDLKKESFFFTLYFVHYKKK
---

---GVVVDSGDGVTHIIXVYEGXSIPHLTXXXDVAGRDVTRYLIKLLLLRGYAFNRTADX
ETIRQIKEKLCYVAYDVQQEMKLASETTVLVENYTLPDGRVIKVGQERFQASEALFNPSL
VDVEGGGVHEQLFDCITKADRDLQQGFYQHIVLSGGSSMYPGL
Translated Amino Acid sequence (All Frames) 
Frame A:
wptgyynsnihhtlfivlll*nltyflfiklnnsl*ir**GDLKKESFFFTLYFVHYKKK
---

---wccxrfr*wchpyhxsx*glxnptfdxxxxccrs*cnslphqiiitpwlrfqsyrrf
xnhpsnqretllcrircstrdeiglrdhcsrrklhpprrscnqswpreipsl*splqsif
grcrrwwcp*tiv*lyhqgrp*sstrflstyrpfwwflyvsrft

Frame B:
gllvittqtstihfl*yyyfki*pifylln*iihckfdnkgi*krnhffslfilytikkk
---

---GVVVDSGDGVTHIIXVYEGXSIPHLTXXXDVAGRDVTRYLIKLLLLRGYAFNRTADX
ETIRQIKEKLCYVAYDVQQEMKLASETTVLVENYTLPDGRVIKVGQERFQASEALFNPSL
VDVEGGGVHEQLFDCITKADRDLQQGFYQHIVLSGGSSMYPGL

Frame C:
aywllqlkhppytfysittlkfdlffiy*ik*fivnsiirgfkkgiiffhslfctl*kk-
--

---vlx*iqvmvspisxqxmrvxqshi*lvvxmlpvvm*lvtssnyyysvvtlsivppix
kpsvkskrnsvmshtmfnkr*nwpqrplfsskttpsqtvv*sklakrdskplkpssihlw
smskvvvsmnnclivsprqtvifnkvsinissflvvplciqvy
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFD448 (VFD448Q) /CSM/VF/VFD4-B/VFD448Q.Seq.d/                        904   0.0
SLB194 (SLB194Q) /CSM/SL/SLB1-D/SLB194Q.Seq.d/                        904   0.0
SFD244 (SFD244Q) /CSM/SF/SFD2-B/SFD244Q.Seq.d/                        904   0.0
FC-AH01 (FC-AH01Q) /CSM/FC/FC-AH/FC-AH01Q.Seq.d/                      904   0.0
CHE249 (CHE249Q) /CSM/CH/CHE2-C/CHE249Q.Seq.d/                        904   0.0
FC-131 (FC-131Q) /CSM/CG/CG-FC/FC-131Q.Seq.d/                         904   0.0
AFJ315 (AFJ315Q) /CSM/AF/AFJ3-A/AFJ315Q.Seq.d/                        904   0.0
AFC587 (AFC587Q) /CSM/AF/AFC5-D/AFC587Q.Seq.d/                        904   0.0
AFJ122 (AFJ122Q) /CSM/AF/AFJ1-A/AFJ122Q.Seq.d/                        898   0.0
AFD277 (AFD277Q) /CSM/AF/AFD2-D/AFD277Q.Seq.d/                        890   0.0
own update 2002. 9.27
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AF095929|AF095929.1 Dictyostelium discoideum actin related protein 2 (arpB) mRNA, complete cds.   904   0.0    3
AC116963|AC116963.2 Dictyostelium discoideum chromosome 2 map 4657875-4914984 strain AX4, complete sequence.   904   0.0    8
AF234974|AF234974.1 Japetella diaphana clone 16 actin gene, partial cds.    50   1e-06  3
AF234972|AF234972.1 Eledonella pygmaea clone 5 actin gene, partial cds.    50   1e-06  3
AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.    38   3e-05  11
AF234976|AF234976.1 Octopus tetricus clone 9 actin gene, partial cds.    46   4e-05  2
AF234919|AF234919.1 Idiosepius pygmaeus clone 43 actin gene, partial cds.    38   4e-05  4
AF435700|AF435700.1 Bulinus truncatus actin gene, exon 2 and partial cds.    50   4e-04  2
AF234968|AF234968.1 Cirrothauma murrayi clone 20 actin gene, partial cds.    42   6e-04  2
CO786098|CO786098.1 BL285B_G03 6-Day Axolotl Tail Blastema (6DAxBL) Ambystoma mexicanum cDNA 5' similar to cytoskeletal beta actin (Sus scrofa), mRNA sequence.    34   8e-04  3
dna update 2004. 9.21
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(O96621) RecName: Full=Actin-related protein 2; AltName: Full=Ac...   287   2e-76
(P53487) RecName: Full=Actin-related protein 2; AltName: Full=Ac...   220   3e-56
BC047530_1(BC047530|pid:none) Mus musculus ARP2 actin-related pr...   219   6e-56
(Q5BL41) RecName: Full=Actin-related protein 2; AltName: Full=Ac...   219   6e-56
AY891099_1(AY891099|pid:none) Synthetic construct Homo sapiens c...   219   6e-56
(Q5M7U6) RecName: Full=Actin-related protein 2; AltName: Full=Ac...   219   6e-56
BC036253_1(BC036253|pid:none) Homo sapiens ARP2 actin-related pr...   219   6e-56
(P53488) RecName: Full=Actin-related protein 2; AltName: Full=Ac...   219   6e-56
(Q7ZTP2) RecName: Full=Actin-related protein 2-A; AltName: Full=...   219   6e-56
AK301634_1(AK301634|pid:none) Homo sapiens cDNA FLJ51655 complet...   219   7e-56
protein update 2009. 3.30
PSORT 

 psg: 0.97  gvh: 0.48  alm: 0.46  top: 0.57  tms: 0.00  mit: 0.17  mip: 0.00
 nuc: 0.06  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: cytoplasmic
	 20.0 %: nuclear
	  4.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal
	  4.0 %: Golgi
	  4.0 %: endoplasmic reticulum
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for CHE249 is cyt
5' end seq. ID CHE249F
5' end seq. 
>CHE249F.Seq
TGGCCTACTGGTTATTACAACTCAAACATCCACCATACACTTTTTATAGTATTACTACTT
TAAAATTTGACCTATTTTTTATTTATTAAATTAAATAATTCATTGTAAATTCGATAATAA
GGGGATTTAAAAAAGGAATCATTTTTTTTCACTCTTTATTTTGTACACTATAAAAAAAAA
ANNNNNNNNNN
Length of 5' end seq. 191
3' end seq. ID CHE249Z
3' end seq. 
>CHE249Z.Seq
NNNNNNNNNNTGGTGTTGTNGTAGATTCAGGTGATGGTGTCACCCATATCATTNCAGTNT
ATGAGGGTTANTCAATCCCACATTTGACTNGTNGTNTNGATGTTGCCGGTCGTGATGTAA
CTCGTTACCTCATCAAATTATTATTACTCCGTGGTTACGCTTTCAATCGTACCGCCGATT
TNGAAACCATCCGTCAAATCAAAGAGAAACTCTGTTATGTCGCATACGATGTTCAACAAG
AGATGAAATTGGCCTCAGAGACCACTGTTCTCGTCGAAAACTACACCCTCCCAGACGGTC
GTGTAATCAAAGTTGGCCAAGAGAGATTCCAAGCCTCTGAAGCCCTCTTCAATCCATCTT
TGGTCGATGTCGAAGGTGGTGGTGTCCATGAACAATTGTTTGATTGTATCACCAAGGCAG
ACCGTGATCTTCAACAAGGTTTCTATCAACATATCGTCCTTTCTGGTGGTTCCTCTATGT
ATCCAGGTTTACC
Length of 3' end seq. 493
Connected seq. ID CHE249P
Connected seq. 
>CHE249P.Seq
TGGCCTACTGGTTATTACAACTCAAACATCCACCATACACTTTTTATAGTATTACTACTT
TAAAATTTGACCTATTTTTTATTTATTAAATTAAATAATTCATTGTAAATTCGATAATAA
GGGGATTTAAAAAAGGAATCATTTTTTTTCACTCTTTATTTTGTACACTATAAAAAAAAA
A----------TGGTGTTGTNGTAGATTCAGGTGATGGTGTCACCCATATCATTNCAGTN
TATGAGGGTTANTCAATCCCACATTTGACTNGTNGTNTNGATGTTGCCGGTCGTGATGTA
ACTCGTTACCTCATCAAATTATTATTACTCCGTGGTTACGCTTTCAATCGTACCGCCGAT
TTNGAAACCATCCGTCAAATCAAAGAGAAACTCTGTTATGTCGCATACGATGTTCAACAA
GAGATGAAATTGGCCTCAGAGACCACTGTTCTCGTCGAAAACTACACCCTCCCAGACGGT
CGTGTAATCAAAGTTGGCCAAGAGAGATTCCAAGCCTCTGAAGCCCTCTTCAATCCATCT
TTGGTCGATGTCGAAGGTGGTGGTGTCCATGAACAATTGTTTGATTGTATCACCAAGGCA
GACCGTGATCTTCAACAAGGTTTCTATCAACATATCGTCCTTTCTGGTGGTTCCTCTATG
TATCCAGGTTTACC
Length of connected seq. 664
Full length Seq ID -
Full length Seq. -
Length of full length seq. -