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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CHE224
Library CH
(Link to library)
Clone ID CHE224
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U12256-1
Original site URL
Representative seq. ID CHE224P
(Link to Original site)
Representative DNA sequence 
>CHE224 (CHE224Q) /CSM/CH/CHE2-A/CHE224Q.Seq.d/
TGTTGGCCTACTGGNATATATATTACTGTACATAGAATTTCAAAAATGATTAATATTGAT
TTTGACAATATTGAACAATCACAACCAACACTCTACGATACACTCGCAAGTGATGTTAAA
TTTTTAAATACACCACATTTCGAATTACCACCAAATGCAAATCCAGGTGATTTTTTAAGA
GAAACATTTGATCATCCATCAGCACCAAAAGCATTGGAATTTGCACATGGTACAACTACA
TTAGCATTTGTATATGGTGGAGGTATTATTGTATCAGNAGATTCAAAATCTACACAAGGA
CCATACGTXXXXXXXXXXAGAACTCATCTCTGTTGCTGCTGCATNTAAAATTTTAGCAAA
NATTGNATATTCATATAAAGGNTACGGTTTATCAATGGGNACTATGATTACTGGTTGGGA
TAAAACTGGACCNCAATTATATTATGTTGATAATGATGGGACTAGATTACATGGTCAACG
TTTTTCATGTGGNTCAGGTTCAACANATGNTTATGGTGTTTTANANACAGGTTTTAAATG
GGATATGAATGATNAANAAGCATATGAACTCGGTCGNANAGCTGTTTATCATGCAACTCA
CAAANANGCTTACTCTGGTGGNGCTATCAATGTTTATCATGTTCAAAAAGATGGTTGGAA
NAAGATTTCTTCTGATGATTGNTTCNAATTANATCNAAAATATTACAATATTGNTCCAAT
TAATAAACCAGNCTAATTAAAANCAACNTTTAAATTTCAATCCCCNCCNCAAGACACACA
CACCTATATTTCATCANAAATATGTATAATTTGTTTTTTCATNAAAAAAAAAAAAANC
sequence update 2002.10.25
Translated Amino Acid sequence 
CWPTGIYITVHRISKMINIDFDNIEQSQPTLYDTLASDVKFLNTPHFELPPNANPGDFLR
ETFDHPSAPKALEFAHGTTTLAFVYGGGIIVSXDSKSTQGPY---

---ELISVAAAXKILAXIXYSYKGYGLSMGTMITGWDKTGPQLYYVDNDGTRLHGQRFSC
GSGSTXXYGVLXTGFKWDMNDXXAYELGRXAVYHATHKXAYSGGAINVYHVQKDGWXKIS
SDDXFXLXXKYYNIXPINKPX*lkxtfkfqsppqdthtyissxiciicffxkkkkx
Translated Amino Acid sequence (All Frames) 
Frame A:
CWPTGIYITVHRISKMINIDFDNIEQSQPTLYDTLASDVKFLNTPHFELPPNANPGDFLR
ETFDHPSAPKALEFAHGTTTLAFVYGGGIIVSXDSKSTQGPY---

---rthlccccx*nfskxxifi*rxrfingxydywlg*nwtxiilc***wd*itwstffm
wxrfnxxlwcfxxrf*mgye*xxsi*trsxsclscnsqxxllwwxyqclscskrwlexdf
f**lxxixskilqyxsn**txlikxnx*isipxxrhthlyfixnmynlffhxkkkx

Frame B:
vgllxyillyiefqk*lililtilnnhnqhstihsqvmlnf*ihhisnyhqmqiqvif*e
khliihqhqkhwnlhmvqlh*hlymvevllyqxiqnlhkdht---

---ELISVAAAXKILAXIXYSYKGYGLSMGTMITGWDKTGPQLYYVDNDGTRLHGQRFSC
GSGSTXXYGVLXTGFKWDMNDXXAYELGRXAVYHATHKXAYSGGAINVYHVQKDGWXKIS
SDDXFXLXXKYYNIXPINKPX*lkxtfkfqsppqdthtyissxiciicffxkkkkx

Frame C:
laywxiyyct*nfknd*y*f*qy*tittntlrytrk*c*ifkyttfrittkcksr*ffkr
ni*ssistksigictwynyisiciwwryycixrfkiytrtir---

---nssllllhxkf*qxlxihikxtvyqwxl*llvgikldxnyimlimmgldymvnvfhv
xqvqxmxmvfxxqvlngi*mxxkhmnsvxxlfimqltxxltlvxlsmfimfkkmvgxrfl
lmixsnxixnitilxqlinqxn*xqxlnfnpxxkthtpifhxkyv*fvfsxkkkk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHE224 (CHE224Q) /CSM/CH/CHE2-A/CHE224Q.Seq.d/                       1338   0.0
SSK437 (SSK437Q) /CSM/SS/SSK4-B/SSK437Q.Seq.d/                        714   0.0
CHE172 (CHE172Q) /CSM/CH/CHE1-C/CHE172Q.Seq.d/                        672   0.0
SSM638 (SSM638Q) /CSM/SS/SSM6-B/SSM638Q.Seq.d/                        601   e-171
SSJ387 (SSJ387Q) /CSM/SS/SSJ3-D/SSJ387Q.Seq.d/                        575   e-163
SSA182 (SSA182Q) /CSM/SS/SSA1-D/SSA182Q.Seq.d/                        575   e-163
SSB110 (SSB110Q) /CSM/SS/SSB1-A/SSB110Q.Seq.d/                        462   e-129
VSD720 (VSD720Q) /CSM/VS/VSD7-A/VSD720Q.Seq.d/                        125   1e-27
VFC412 (VFC412Q) /CSM/VF/VFC4-A/VFC412Q.Seq.d/                         44   0.003
VFK885 (VFK885Q) /CSM/VF/VFK8-D/VFK885Q.Seq.d/                         42   0.013
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AX489186|AX489186.1 Sequence 6486 from Patent WO02053728.          46   9e-11  4
BG585459|BG585459.1 EST487223 MHAM Medicago truncatula/Glomus versiforme mixed EST library cDNA clone pMHAM-30A15 5' end, mRNA sequence.    50   3e-04  3
AX830652|AX830652.1 Sequence 1372 from Patent WO03072602.          44   5e-04  3
AX819622|AX819622.1 Sequence 1372 from Patent EP1338608.           44   5e-04  3
AX595502|AX595502.1 Sequence 1156 from Patent EP1258494.           44   5e-04  3
M96667|M96667.1 Saccharomyces cerevisiae proteosome-related protein (PRG1) gene, complete cds.    44   0.001  3
X68662|X68662.1 S.cerevisiae PRE2 gene.                            44   0.002  3
CD073482|CD073482.1 MA3-0001U-L244-H07-U.B MA3-0001 Schistosoma mansoni cDNA clone MA3-0001U-L244-H07.B, mRNA sequence.    36   0.005  4
CD074032|CD074032.1 MA3-0001U-M278-D08-U.G MA3-0001 Schistosoma mansoni cDNA clone MA3-0001U-M278-D08.G, mRNA sequence.    36   0.007  4
CD073619|CD073619.1 MA3-0001U-M273-E05-U.B MA3-0001 Schistosoma mansoni cDNA clone MA3-0001U-M273-E05.B, mRNA sequence.    36   0.007  4
dna update 2004. 9.21
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

BX323833_6(BX323833|pid:none) Pig DNA sequence from clone PigI-5...   156   1e-45
AY050871_1(AY050871|pid:none) Arabidopsis thaliana putative prot...   156   2e-45
(O23717) RecName: Full=Proteasome subunit beta type-5-A;        ...   156   5e-45
DQ413236_1(DQ413236|pid:none) Acyrthosiphon pisum isolate WHAP04...   155   7e-45
(O24361) RecName: Full=Proteasome subunit beta type-5;          ...   159   3e-44
(Q5W416) RecName: Full=Proteasome subunit beta type-8;          ...   151   3e-43
AF217726_1(AF217726|pid:none) Drosophila melanogaster 20S protea...   157   4e-43
AY813054_1(AY813054|pid:none) Schistosoma japonicum SJCHGC05978 ...   151   5e-43
AJ630364_5(AJ630364|pid:none) Canis familiaris major histocompat...   151   6e-43
AE013599_1217(AE013599|pid:none) Drosophila melanogaster chromos...   157   8e-43
protein update 2009. 3.30
PSORT 

 psg: 0.87  gvh: 0.30  alm: 0.38  top: 0.43  tms: 0.00  mit: 0.29  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: cytoplasmic
	 24.0 %: nuclear
	 20.0 %: cytoskeletal
	  4.0 %: extracellular, including cell wall
	  4.0 %: peroxisomal
	  4.0 %: vacuolar
	  4.0 %: mitochondrial
	  4.0 %: plasma membrane

>> prediction for CHE224 is cyt
5' end seq. ID CHE224F
5' end seq. 
>CHE224F.Seq
TGTTGGCCTACTGGNATATATATTACTGTACATAGAATTTCAAAAATGATTAATATTGAT
TTTGACAATATTGAACAATCACAACCAACACTCTACGATACACTCGCAAGTGATGTTAAA
TTTTTAAATACACCACATTTCGAATTACCACCAAATGCAAATCCAGGTGATTTTTTAAGA
GAAACATTTGATCATCCATCAGCACCAAAAGCATTGGAATTTGCACATGGTACAACTACA
TTAGCATTTGTATATGGTGGAGGTATTATTGTATCAGNAGATTCAAAATCTACACAAGGA
CCATACGTNNNNNNNNNN
Length of 5' end seq. 318
3' end seq. ID CHE224Z
3' end seq. 
>CHE224Z.Seq
NNNNNNNNNNAGAACTCATCTCTGTTGCTGCTGCATNTAAAATTTTAGCAAANATTGNAT
ATTCATATAAAGGNTACGGTTTATCAATGGGNACTATGATTACTGGTTGGGATAAAACTG
GACCNCAATTATATTATGTTGATAATGATGGGACTAGATTACATGGTCAACGTTTTTCAT
GTGGNTCAGGTTCAACANATGNTTATGGTGTTTTANANACAGGTTTTAAATGGGATATGA
ATGATNAANAAGCATATGAACTCGGTCGNANAGCTGTTTATCATGCAACTCACAAANANG
CTTACTCTGGTGGNGCTATCAATGTTTATCATGTTCAAAAAGATGGTTGGAANAAGATTT
CTTCTGATGATTGNTTCNAATTANATCNAAAATATTACAATATTGNTCCAATTAATAAAC
CAGNCTAATTAAAANCAACNTTTAAATTTCAATCCCCNCCNCAAGACACACACACCTATA
TTTCATCANAAATATGTATAATTTGTTTTTTCATNAAAAAAAAAAAAANC
Length of 3' end seq. 530
Connected seq. ID CHE224P
Connected seq. 
>CHE224P.Seq
TGTTGGCCTACTGGNATATATATTACTGTACATAGAATTTCAAAAATGATTAATATTGAT
TTTGACAATATTGAACAATCACAACCAACACTCTACGATACACTCGCAAGTGATGTTAAA
TTTTTAAATACACCACATTTCGAATTACCACCAAATGCAAATCCAGGTGATTTTTTAAGA
GAAACATTTGATCATCCATCAGCACCAAAAGCATTGGAATTTGCACATGGTACAACTACA
TTAGCATTTGTATATGGTGGAGGTATTATTGTATCAGNAGATTCAAAATCTACACAAGGA
CCATACGT----------AGAACTCATCTCTGTTGCTGCTGCATNTAAAATTTTAGCAAA
NATTGNATATTCATATAAAGGNTACGGTTTATCAATGGGNACTATGATTACTGGTTGGGA
TAAAACTGGACCNCAATTATATTATGTTGATAATGATGGGACTAGATTACATGGTCAACG
TTTTTCATGTGGNTCAGGTTCAACANATGNTTATGGTGTTTTANANACAGGTTTTAAATG
GGATATGAATGATNAANAAGCATATGAACTCGGTCGNANAGCTGTTTATCATGCAACTCA
CAAANANGCTTACTCTGGTGGNGCTATCAATGTTTATCATGTTCAAAAAGATGGTTGGAA
NAAGATTTCTTCTGATGATTGNTTCNAATTANATCNAAAATATTACAATATTGNTCCAAT
TAATAAACCAGNCTAATTAAAANCAACNTTTAAATTTCAATCCCCNCCNCAAGACACACA
CACCTATATTTCATCANAAATATGTATAATTTGTTTTTTCATNAAAAAAAAAAAAANC
Length of connected seq. 828
Full length Seq ID -
Full length Seq. -
Length of full length seq. -