>CHE214 (CHE214Q) /CSM/CH/CHE2-A/CHE214Q.Seq.d/
TGATTGATCATTTTATTATTAAAGGNGTAAAACTTTCAACACAAATTGATATGTTTTGTC
AAATATTTAAAATTCAAGAAGACCCAATTAAATACTCTTTACTAXXXXXXXXXX

IDHFIIKGVKLSTQIDMFCQIFKIQEDPIKYSL---

Frame A:
*liilllkx*nfqhklicfvkylkfkktqlntly---


Frame B:
d*sfyy*rxktfntn*yvlsni*nsrrpn*ilft---


Frame C:
IDHFIIKGVKLSTQIDMFCQIFKIQEDPIKYSL---

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHE214 (CHE214Q) /CSM/CH/CHE2-A/CHE214Q.Seq.d/                        200   3e-51
CFH357 (CFH357Q) /CSM/CF/CFH3-C/CFH357Q.Seq.d/                         36   0.099
VFO254 (VFO254Q) /CSM/VF/VFO2-C/VFO254Q.Seq.d/                         32   1.5
VFM332 (VFM332Q) /CSM/VF/VFM3-B/VFM332Q.Seq.d/                         32   1.5
VFL579 (VFL579Q) /CSM/VF/VFL5-D/VFL579Q.Seq.d/                         32   1.5
VFH238 (VFH238Q) /CSM/VF/VFH2-B/VFH238Q.Seq.d/                         32   1.5
VFG757 (VFG757Q) /CSM/VF/VFG7-C/VFG757Q.Seq.d/                         32   1.5
VFG711 (VFG711Q) /CSM/VF/VFG7-A/VFG711Q.Seq.d/                         32   1.5
VFF874 (VFF874Q) /CSM/VF/VFF8-D/VFF874Q.Seq.d/                         32   1.5
VFF842 (VFF842Q) /CSM/VF/VFF8-B/VFF842Q.Seq.d/                         32   1.5

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC100265|AC100265.1 Mus musculus clone RP23-66M6, LOW-PASS SEQUENCE SAMPLING.    34   0.004  4
BX649502|BX649502.4 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone DKEY-204F11.    34   0.004  8
AC100980|AC100980.1 Mus musculus clone RP23-76L4, LOW-PASS SEQUENCE SAMPLING.    38   0.008  3
AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.    36   0.012  9
BX640460|BX640460.3 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone DKEY-240I15.    32   0.015  8
AC069593|AC069593.3 Homo sapiens chromosome 4 clone RP11-632F23 map 4, WORKING DRAFT SEQUENCE, 27 unordered pieces.    32   0.020  7
AY533599|AY533599.1 Diabrotica porracea 12S ribosomal RNA gene, partial sequence; mitochondrial.    32   0.020  3
AY533603|AY533603.1 Diabrotica virgifera virgifera 12S ribosomal RNA gene, partial sequence; mitochondrial.    32   0.021  3
AY533602|AY533602.1 Diabrotica virgifera zeae 12S ribosomal RNA gene, partial sequence; mitochondrial.    32   0.022  3
AC130243|AC130243.5 Rattus norvegicus clone CH230-301P10, *** SEQUENCING IN PROGRESS ***, 1 ordered piece.    34   0.081  7

 ***** No hits found ******

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3039690
Number of Hits to DB: 128,137,531

 psg: 0.77  gvh: 0.22  alm: 0.48  top: 0.53  tms: 0.00  mit: 0.25  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: nuclear
	 28.0 %: cytoplasmic
	 12.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: extracellular, including cell wall

>> prediction for CHE214 is nuc

>CHE214F.Seq
TGATTGATCATTTTATTATTAAAGGNGTAAAACTTTCAACACAAATTGATATGTTTTGTC
AAATATTTAAAATTCAAGAAGACCCAATTAAATACTCTTTACTANNNNNNNNNN