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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFF518
Library AF
(Link to library)
Clone ID AFF518
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U14936-1
Original site URL
Representative seq. ID AFF518P
(Link to Original site)
Representative DNA sequence 
>AFF518 (AFF518Q) /CSM/AF/AFF5-A/AFF518Q.Seq.d/
ATTCCATTATTTACACATACACTTACACACATCATCATCATAATCATATATTTTTAATAG
ATATTGATATATATATTTAAAAAAATAAAATAAAATAAAATAAAAAATGTCAACAGAGGA
AACAAAAAAAGAGCAAGCACTTCAAAATTATAGAGATCGTTTAATAGAACATAAAAATGC
AGAACTTAGATTAAATAAAGCTCACGAACTTATTAAAAAATTAANAAAAGATTTTCAAAA
AACAGAGGATCACATTAAAACTATTCAATATATTGGTGAAATAATTGGTGAAGTTTTAAG
ATCATTAGATGAAGAAAGATTTATTGTTAAAGCATGTAATGGACCAAGATATGTTGTTAG
ATGTGCAAATTATCAAGATAAAGCACATTTATTAGTACCAGGTGCACGTGTTACATTAGA
TTTAACAACATTAACAATTTTAAAGATATTACCACGTGAAGTTGATCCAATCATTTTCAA
TATGACAGCTGAATCACCAGGTAGTGTATCGTATGGTGAAATTGGTGGATTAAGTAATCA
AATCAGAGAGTTAAGAGAAGTTGTAGAGTTACCATTGATGATTCCAXXXXXXXXXXTCGT
TTAGATAGAAAAATAGAAATTCCTTTACCAAATGAAGCTGGTCGTGTCGATGTTCTTAAA
ATTCATGCTGCAAATATCACCAAACATGGTGATGTAGATTATGAAGCTATCGCTAAATTA
GCTGATGGTTTCAATGCTGCTGATTTACGTAATGTATGTACTGAAGCTGGTATGTTTGCA
ATTAGAGCTGAAAGAGATTATGTAATGGAAGAAGACTTTATGAAAGCTGTTAGAAAATGT
CAAGAAGCAAAGAAATTAGAAGGTAAACTCGATTATCAAAAAGTTTAAAAAAAAATTTAT
AATTTCCTTTTTTTAGGATTAATNCTTAAAAATAATAATAATAATAA
sequence update 2001. 6. 2
Translated Amino Acid sequence 
fhylhihlhtsss*syifnry*yiylkk*nkik*KMSTEETKKEQALQNYRDRLIEHKNA
ELRLNKAHELIKKLXKDFQKTEDHIKTIQYIGEIIGEVLRSLDEERFIVKACNGPRYVVR
CANYQDKAHLLVPGARVTLDLTTLTILKILPREVDPIIFNMTAESPGSVSYGEIGGLSNQ
IRELREVVELPLMIP---

---RLDRKIEIPLPNEAGRVDVLKIHAANITKHGDVDYEAIAKLADGFNAADLRNVCTEA
GMFAIRAERDYVMEEDFMKAVRKCQEAKKLEGKLDYQKV*kkiynflflglxlknnnnn
Translated Amino Acid sequence (All Frames) 
Frame A:
iplfthtlthiiiiiiyf**iliyifkkik*nkiknvnrgnkkrastskl*rsfnrt*kc
rt*ik*ssrty*kixkrfsknrgsh*nysiyw*nnw*sfkiir*rkiyc*sm*wtkicc*
mcklsr*stfistrctcyirfnninnfkditt*s*snhfqyds*itr*civw*nwwik*s
nqrvkrscrvtidds---

---sfr*knrnsftk*swscrcs*nscckyhqtw*crl*syr*is*wfqcc*ft*cmy*s
wyvcn*s*krlcngrrlyesc*kmsrskeirr*trlskslkknl*fpffrinx*k*****

Frame B:
fhylhihlhtsss*syifnry*yiylkk*nkik*KMSTEETKKEQALQNYRDRLIEHKNA
ELRLNKAHELIKKLXKDFQKTEDHIKTIQYIGEIIGEVLRSLDEERFIVKACNGPRYVVR
CANYQDKAHLLVPGARVTLDLTTLTILKILPREVDPIIFNMTAESPGSVSYGEIGGLSNQ
IRELREVVELPLMIP---

---RLDRKIEIPLPNEAGRVDVLKIHAANITKHGDVDYEAIAKLADGFNAADLRNVCTEA
GMFAIRAERDYVMEEDFMKAVRKCQEAKKLEGKLDYQKV*kkiynflflglxlknnnnn

Frame C:
siiytytythhhhnhiflididiyi*knkik*nkkcqqrkqkkskhfkiieiv**nikmq
nld*ikltnllkn*xkifkkqritlklfnilvk*lvkf*dh*mkkdlllkhvmdqdmlld
vqiikikhiy*yqvhvlh*i*qh*qf*ryyhvkliqsfsi*qlnhqvvyrmvklvd*vik
ses*ekl*syh**f---

---v*iek*kflyqmklvvsmflkfmlqispnmvm*imklsln*lmvsmlliyvmyvlkl
vclqlelkeim*wkktl*kllenvkkqrn*kvnsiikkfkkkfiisff*d*xlkiiiii
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFF518 (AFF518Q) /CSM/AF/AFF5-A/AFF518Q.Seq.d/                       1437   0.0
VFM122 (VFM122Q) /CSM/VF/VFM1-A/VFM122Q.Seq.d/                        902   0.0
AFN612 (AFN612Q) /CSM/AF/AFN6-A/AFN612Q.Seq.d/                        902   0.0
AFF540 (AFF540Q) /CSM/AF/AFF5-B/AFF540Q.Seq.d/                        902   0.0
AFE753 (AFE753Q) /CSM/AF/AFE7-C/AFE753Q.Seq.d/                        902   0.0
AFD621 (AFD621Q) /CSM/AF/AFD6-A/AFD621Q.Seq.d/                        902   0.0
AFD486 (AFD486Q) /CSM/AF/AFD4-D/AFD486Q.Seq.d/                        902   0.0
SFJ688 (SFJ688Q) /CSM/SF/SFJ6-D/SFJ688Q.Seq.d/                        898   0.0
SFG752 (SFG752Q) /CSM/SF/SFG7-C/SFG752Q.Seq.d/                        898   0.0
VFA243 (VFA243Q) /CSM/VF/VFA2-B/VFA243Q.Seq.d/                        894   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

BU495650|BU495650.1 PfESToab75g10.y1 Plasmodium falciparum 3D7 asexual cDNA PlasmodiummRNA sequence.    78   2e-17  2
BQ596428|BQ596428.1 PfESToab33f08.y1 Plasmodium falciparum 3D7 asexual cDNA Plasmodium falciparum cDNA 5' similar to TR:Q9W414 Q9W414 RPT4 PROTEIN. ;, mRNA sequence.    78   2e-17  2
BI815262|BI815262.1 PfESToaa16d01.y1 Plasmodium falciparum 3D7 asexual cDNA Plasmodium falciparum cDNA 5' similar to TR:Q9W414 Q9W414 RPT4 PROTEIN. ;, mRNA sequence.    78   2e-17  2
BI815862|BI815862.1 PfESToaa33e12.y1 Plasmodium falciparum 3D7 asexual cDNA Plasmodium falciparum cDNA 5' similar to SW:PRSX_CAEEL O17071 PROBABLE 26S PROTEASE REGULATORY SUBUNIT S10B. [1] ;, mRNA sequence.    78   3e-17  2
BQ577133|BQ577133.1 PfESToab14c09.y1 Plasmodium falciparum 3D7 asexual cDNA Plasmodium falciparum cDNA 5' similar to SW:PRSX_CAEEL O17071 PROBABLE 26S PROTEASE REGULATORY SUBUNIT S10B. [1] ;, mRNA sequence.    70   7e-15  2
BC057997|BC057997.1 Mus musculus proteasome (prosome, macropain) 26S subunit, ATPase, 6, mRNA (cDNA clone IMAGE:5342248), partial cds.    50   4e-13  6
BD301526|BD301526.1 Identification and Use of Molecules Implicated in Pain.    42   4e-13  5
AX683192|AX683192.1 Sequence 166 from Patent EP1279744.            42   4e-13  5
U36395|U36395.1 Spermophilus tridecemlineatus 26S Proteasome S.U. CADp44 - a conserved  ATPase domain protein of 44kDa (CADp44) gene, complete cds.    42   4e-13  5
BC043044|BC043044.1 Mus musculus, RIKEN cDNA 2300001E01 gene, clone IMAGE:5701431, mRNA, partial cds.    50   4e-13  6
dna update 2004. 4.24
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY892558_1(AY892558|pid:none) Synthetic construct Homo sapiens c...   162   1e-38
CQ840920_1(CQ840920|pid:none) Sequence 15 from Patent EP1439230....   162   1e-38
BC057997_1(BC057997|pid:none) Mus musculus proteasome (prosome, ...   162   1e-38
BC073644_1(BC073644|pid:none) Xenopus laevis similar to proteaso...   162   1e-38
BC064227_1(BC064227|pid:none) Xenopus tropicalis proteasome (pro...   162   1e-38
EF103411_1(EF103411|pid:none) Haliotis discus discus psmc6 prote...   162   2e-38
AK014354_1(AK014354|pid:none) Mus musculus 17 days embryo head c...   161   2e-38
BC097594_1(BC097594|pid:none) Xenopus laevis hypothetical protei...   161   3e-38
BC107950_1(BC107950|pid:none) Rattus norvegicus proteasome (pros...   160   4e-38
AX885317_1(AX885317|pid:none) Sequence 1180 from Patent EP1033401.    160   7e-38
protein update 2009. 4.20
PSORT 

 psg: 0.44  gvh: 0.06  alm: 0.41  top: 0.53  tms: 0.00  mit: 0.26  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: nuclear
	 32.0 %: cytoplasmic
	  8.0 %: cytoskeletal
	  4.0 %: mitochondrial

>> prediction for AFF518 is nuc
5' end seq. ID AFF518F
5' end seq. 
>AFF518F.Seq
ATTCCATTATTTACACATACACTTACACACATCATCATCATAATCATATATTTTTAATAG
ATATTGATATATATATTTAAAAAAATAAAATAAAATAAAATAAAAAATGTCAACAGAGGA
AACAAAAAAAGAGCAAGCACTTCAAAATTATAGAGATCGTTTAATAGAACATAAAAATGC
AGAACTTAGATTAAATAAAGCTCACGAACTTATTAAAAAATTAANAAAAGATTTTCAAAA
AACAGAGGATCACATTAAAACTATTCAATATATTGGTGAAATAATTGGTGAAGTTTTAAG
ATCATTAGATGAAGAAAGATTTATTGTTAAAGCATGTAATGGACCAAGATATGTTGTTAG
ATGTGCAAATTATCAAGATAAAGCACATTTATTAGTACCAGGTGCACGTGTTACATTAGA
TTTAACAACATTAACAATTTTAAAGATATTACCACGTGAAGTTGATCCAATCATTTTCAA
TATGACAGCTGAATCACCAGGTAGTGTATCGTATGGTGAAATTGGTGGATTAAGTAATCA
AATCAGAGAGTTAAGAGAAGTTGTAGAGTTACCATTGATGATTCCA----------
Length of 5' end seq. 586
3' end seq. ID AFF518Z
3' end seq. 
>AFF518Z.Seq
----------TCGTTTAGATAGAAAAATAGAAATTCCTTTACCAAATGAAGCTGGTCGTG
TCGATGTTCTTAAAATTCATGCTGCAAATATCACCAAACATGGTGATGTAGATTATGAAG
CTATCGCTAAATTAGCTGATGGTTTCAATGCTGCTGATTTACGTAATGTATGTACTGAAG
CTGGTATGTTTGCAATTAGAGCTGAAAGAGATTATGTAATGGAAGAAGACTTTATGAAAG
CTGTTAGAAAATGTCAAGAAGCAAAGAAATTAGAAGGTAAACTCGATTATCAAAAAGTTT
AAAAAAAAATTTATAATTTCCTTTTTTTAGGATTAATNCTTAAAAATAATAATAATAATA
A
Length of 3' end seq. 351
Connected seq. ID AFF518P
Connected seq. 
>AFF518P.Seq
ATTCCATTATTTACACATACACTTACACACATCATCATCATAATCATATATTTTTAATAG
ATATTGATATATATATTTAAAAAAATAAAATAAAATAAAATAAAAAATGTCAACAGAGGA
AACAAAAAAAGAGCAAGCACTTCAAAATTATAGAGATCGTTTAATAGAACATAAAAATGC
AGAACTTAGATTAAATAAAGCTCACGAACTTATTAAAAAATTAANAAAAGATTTTCAAAA
AACAGAGGATCACATTAAAACTATTCAATATATTGGTGAAATAATTGGTGAAGTTTTAAG
ATCATTAGATGAAGAAAGATTTATTGTTAAAGCATGTAATGGACCAAGATATGTTGTTAG
ATGTGCAAATTATCAAGATAAAGCACATTTATTAGTACCAGGTGCACGTGTTACATTAGA
TTTAACAACATTAACAATTTTAAAGATATTACCACGTGAAGTTGATCCAATCATTTTCAA
TATGACAGCTGAATCACCAGGTAGTGTATCGTATGGTGAAATTGGTGGATTAAGTAATCA
AATCAGAGAGTTAAGAGAAGTTGTAGAGTTACCATTGATGATTCCA----------TCGT
TTAGATAGAAAAATAGAAATTCCTTTACCAAATGAAGCTGGTCGTGTCGATGTTCTTAAA
ATTCATGCTGCAAATATCACCAAACATGGTGATGTAGATTATGAAGCTATCGCTAAATTA
GCTGATGGTTTCAATGCTGCTGATTTACGTAATGTATGTACTGAAGCTGGTATGTTTGCA
ATTAGAGCTGAAAGAGATTATGTAATGGAAGAAGACTTTATGAAAGCTGTTAGAAAATGT
CAAGAAGCAAAGAAATTAGAAGGTAAACTCGATTATCAAAAAGTTTAAAAAAAAATTTAT
AATTTCCTTTTTTTAGGATTAATNCTTAAAAATAATAATAATAATAA
Length of connected seq. 937
Full length Seq ID -
Full length Seq. -
Length of full length seq. -