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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CHD465
Library CH
(Link to library)
Clone ID CHD465
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U14919-1
Original site URL
Representative seq. ID CHD465P
(Link to Original site)
Representative DNA sequence 
>CHD465 (CHD465Q) /CSM/CH/CHD4-C/CHD465Q.Seq.d/
ACACAAGTAATTATATAATATACATATACATATAAAATGGGTTCAGTATCAGGTAATACA
CCATTAAAAGAAGTTGATAATGAAATCTTTGAATTAATGAATAGAGAAAAAGATAGACAA
TTTAAAGGTTTAGAATTAATTGCCTCTGAAAATTTTACATCAAGAGCAGTTATGGAAGCA
TTAGGATCACATTTTACAAATAAATATGCAGAAGGTTATCCAGGTTCAAGATATTATGGT
GGTACAGAAGTTGTTGATGAATTAGAAACTCTTTGTCAAAAGAGAGCATTAAAAGCATTC
CGTTTGGATGAGAGTAAATGGGGTGTTAATGTTCAACCATACAGTGGTAGTCCAGCCAAT
TTCGCAGTTTACACAGCATTATTAAGACCACACGATCGTATAATGGGTTTAGATTTACCA
TCAGGTGGTCATTTAACTCATGGTTATCAAACCGATAAGAAGAAGATTTCAGCATCATCA
ATCTTTTTCGAATCAATGCCATATCAAATTGGTGCTGATGGTTTAATCGATTATCAACGT
TTAGAAGAGAATGCATTXXXXXXXXXXGTACGACATTGAATCAAAGATCAATTTCGCAGT
TTTCCCATCACTCCAAGGTGGATCACATGAAAATGTTATTGCTGGTGTTGCTGTTGCTCT
CAAAGAAGCCGACTCTCAAGAATTCAAAGAGTATGCCCTTCAAGTTAAAAAGAATGCCGC
TGCCATTGGTAATGCTCTCATGAACAAAGGTTATAAATTGGTTACAAATGGTACTGACAA
TCATTTAATTCTTTGGGATTTAAGACCAAAGGAACTCACTGGTAATAAATTTGAAAAAGC
TGCCGATATCGCTAATATCACCGTCAATAAAAATGCCGTCCATGGTGATACCAATGCAAT
TTCACCAGGTGGTATTCGTATTGGTTCATCTGCTCTCACAAGTAGAGGTTTAAAGGAAGC
CGATTTTGAAAAGATTGCTGATTTCCTTGATAGAATCGTTTCAATCTCTCTTGAAATTCA
AGGTCGTGTCGGTAAGANATTGGTTGATTTTTGTTGTTTTAATTNATTTTTNTTTTTTTN
TTTTTNNTTTTTTNTTTTTTT
sequence update 2002.10.25
Translated Amino Acid sequence 
tqvii*YTYTYKMGSVSGNTPLKEVDNEIFELMNREKDRQFKGLELIASENFTSRAVMEA
LGSHFTNKYAEGYPGSRYYGGTEVVDELETLCQKRALKAFRLDESKWGVNVQPYSGSPAN
FAVYTALLRPHDRIMGLDLPSGGHLTHGYQTDKKKISASSIFFESMPYQIGADGLIDYQR
LEENA---

---YDIESKINFAVFPSLQGGSHENVIAGVAVALKEADSQEFKEYALQVKKNAAAIGNAL
MNKGYKLVTNGTDNHLILWDLRPKELTGNKFEKAADIANITVNKNAVHGDTNAISPGGIR
IGSSALTSRGLKEADFEKIADFLDRIVSISLEIQGRVGKXLVDFCCFNXFXFFXFXFFXF
Translated Amino Acid sequence (All Frames) 
Frame A:
tqvii*YTYTYKMGSVSGNTPLKEVDNEIFELMNREKDRQFKGLELIASENFTSRAVMEA
LGSHFTNKYAEGYPGSRYYGGTEVVDELETLCQKRALKAFRLDESKWGVNVQPYSGSPAN
FAVYTALLRPHDRIMGLDLPSGGHLTHGYQTDKKKISASSIFFESMPYQIGADGLIDYQR
LEENA---

---vrh*ikdqfrsfpitprwit*kcycwccccsqrsrlsriqrvcpss*kecrchw*cs
heqrl*igykwy*qsfnslgfktkgthw**i*kscryr*yhrq*kcrpw*yqcnftrwys
ywficshk*rfkgsrf*kdc*fp**nrfnls*nsrscr*xig*fllf*xifxfxfxxfxf
f

Frame B:
hk*lynihihikwvqyqvihh*kklimksln**iekkidnlkv*n*lplkilhqeqlwkh
*dhilqinmqkviqvqdimvvqkllmn*klfvkreh*khsvwmrvngvlmfnhtvvvqpi
sqftqhy*dhtiv*wv*iyhqvvi*lmvikpirrrfqhhqsfsnqchiklvlmv*siinv
*krmh---

---YDIESKINFAVFPSLQGGSHENVIAGVAVALKEADSQEFKEYALQVKKNAAAIGNAL
MNKGYKLVTNGTDNHLILWDLRPKELTGNKFEKAADIANITVNKNAVHGDTNAISPGGIR
IGSSALTSRGLKEADFEKIADFLDRIVSISLEIQGRVGKXLVDFCCFNXFXFFXFXFFXF

Frame C:
tsnyiiyiyi*ngfsir*ytikrs***nl*ine*rkr*ti*rfrincl*kfyikssygsi
ritfyk*icrrlsrfkilwwyrsc**irnslskesiksipfg*e*mgc*cstiqw*ssqf
rslhsiikttrsyngfrftirwsfnswlsnr*eedfsiinlfrinaisnwc*wfnrlstf
rreci---

---ttlnqrsisqfshhskvdhmkmlllvllllskkptlknsksmpfklkrmplplvmls
*tkvinwlqmvltii*ffgi*dqrnslvinlkklpislispsikmpsmvipmqfhqvvfv
lvhllsqvev*rkpilkrllisliesfqsllkfkvvsvrxwlifvvlixfxffxfxfxff
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHD465 (CHD465Q) /CSM/CH/CHD4-C/CHD465Q.Seq.d/                       2044   0.0
VHA876 (VHA876Q) /CSM/VH/VHA8-D/VHA876Q.Seq.d/                       1104   0.0
VFN540 (VFN540Q) /CSM/VF/VFN5-B/VFN540Q.Seq.d/                       1104   0.0
VFM764 (VFM764Q) /CSM/VF/VFM7-C/VFM764Q.Seq.d/                       1104   0.0
VFK411 (VFK411Q) /CSM/VF/VFK4-A/VFK411Q.Seq.d/                       1104   0.0
VFE478 (VFE478Q) /CSM/VF/VFE4-D/VFE478Q.Seq.d/                       1104   0.0
VFC539 (VFC539Q) /CSM/VF/VFC5-B/VFC539Q.Seq.d/                       1104   0.0
VFB742 (VFB742Q) /CSM/VF/VFB7-B/VFB742Q.Seq.d/                       1104   0.0
SFK139 (SFK139Q) /CSM/SF/SFK1-B/SFK139Q.Seq.d/                       1104   0.0
SFI686 (SFI686Q) /CSM/SF/SFI6-D/SFI686Q.Seq.d/                       1104   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AF009966|AF009966.1 Candida albicans serine hydroxymethyl transferase II (SHMII) gene, complete cds.    58   4e-16  4
BI418085|BI418085.1 LjNEST24d6r Lotus japonicus nodule library 5 and 7 week-old Lotus japonicus cDNA 5', mRNA sequence.    60   1e-13  3
CB408430|CB408430.1 IPG006D12_249762 JH III-treated male I. pini midguts Ips pini cDNA clone IPG006D12 5, mRNA sequence.    62   2e-12  3
BP187516|BP187516.1 Dugesia japonica cDNA, clone: 00331_HN, expressed in the head.    52   4e-12  3
BQ489771|BQ489771.1 14-E9425-006-009-K03-T3 Sugar beet MPIZ-ADIS-006 Lambda Zap II library Beta vulgaris cDNA clone K-3-9, mRNA sequence.    50   6e-12  3
CV017773|CV017773.1 tbt_009554 Normalized Nicotiana tabacum cDNA library Nicotiana tabacum cDNA clone tbt_009554 5', mRNA sequence.    66   8e-12  2
AL408772|AL408772.1 T7 end of clone AV0AA010F02 of library AV0AA from strain CBS 379 of Saccharomyces exiguus.    58   6e-11  3
AI960890|AI960890.1 sc92a10.y1 Gm-c1019 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1019-787 5' similar to TR:O04952 O04952 SERINE HYDROXYMETHYLTRANSFERASE ;, mRNA sequence.    52   6e-11  3
CK265100|CK265100.1 EST711178 potato abiotic stress cDNA library Solanum tuberosum cDNA clone POABW75 5' end, mRNA sequence.    46   1e-10  4
BX842644|BX842644.1 Mycoplasma mycoides subsp. mycoides SC genomic DNA, complete  sequence; segment 3/4.    58   3e-10  14
dna update 2004. 9.19
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

BC066496_1(BC066496|pid:none) Danio rerio serine hydroxymethyltr...   253   8e-66
AK302834_1(AK302834|pid:none) Homo sapiens cDNA FLJ60988 complet...   253   8e-66
AK223552_1(AK223552|pid:none) Homo sapiens mRNA for serine hydro...   253   8e-66
L23928_3(L23928|pid:none) Homo sapiens serine hydroxymethyltrans...   253   8e-66
BC022874_1(BC022874|pid:none) Homo sapiens serine hydroxymethylt...   253   8e-66
(P34896) RecName: Full=Serine hydroxymethyltransferase, cytosoli...   253   8e-66
AY850381_1(AY850381|pid:none) Danio rerio serine hydroxymethyltr...   253   8e-66
(P07511) RecName: Full=Serine hydroxymethyltransferase, cytosoli...   252   1e-65
BC055527_1(BC055527|pid:none) Danio rerio serine hydroxymethyltr...   249   9e-65
(P50431) RecName: Full=Serine hydroxymethyltransferase, cytosoli...   248   2e-64
protein update 2009. 3.30
PSORT 

 psg: 0.81  gvh: 0.40  alm: 0.38  top: 0.53  tms: 0.00  mit: 0.30  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 28.0 %: nuclear
	 12.0 %: mitochondrial
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for CHD465 is cyt
5' end seq. ID CHD465F
5' end seq. 
>CHD465F.Seq
ACACAAGTAATTATATAATATACATATACATATAAAATGGGTTCAGTATCAGGTAATACA
CCATTAAAAGAAGTTGATAATGAAATCTTTGAATTAATGAATAGAGAAAAAGATAGACAA
TTTAAAGGTTTAGAATTAATTGCCTCTGAAAATTTTACATCAAGAGCAGTTATGGAAGCA
TTAGGATCACATTTTACAAATAAATATGCAGAAGGTTATCCAGGTTCAAGATATTATGGT
GGTACAGAAGTTGTTGATGAATTAGAAACTCTTTGTCAAAAGAGAGCATTAAAAGCATTC
CGTTTGGATGAGAGTAAATGGGGTGTTAATGTTCAACCATACAGTGGTAGTCCAGCCAAT
TTCGCAGTTTACACAGCATTATTAAGACCACACGATCGTATAATGGGTTTAGATTTACCA
TCAGGTGGTCATTTAACTCATGGTTATCAAACCGATAAGAAGAAGATTTCAGCATCATCA
ATCTTTTTCGAATCAATGCCATATCAAATTGGTGCTGATGGTTTAATCGATTATCAACGT
TTAGAAGAGAATGCATTNNNNNNNNNN
Length of 5' end seq. 567
3' end seq. ID CHD465Z
3' end seq. 
>CHD465Z.Seq
NNNNNNNNNNGTACGACATTGAATCAAAGATCAATTTCGCAGTTTTCCCATCACTCCAAG
GTGGATCACATGAAAATGTTATTGCTGGTGTTGCTGTTGCTCTCAAAGAAGCCGACTCTC
AAGAATTCAAAGAGTATGCCCTTCAAGTTAAAAAGAATGCCGCTGCCATTGGTAATGCTC
TCATGAACAAAGGTTATAAATTGGTTACAAATGGTACTGACAATCATTTAATTCTTTGGG
ATTTAAGACCAAAGGAACTCACTGGTAATAAATTTGAAAAAGCTGCCGATATCGCTAATA
TCACCGTCAATAAAAATGCCGTCCATGGTGATACCAATGCAATTTCACCAGGTGGTATTC
GTATTGGTTCATCTGCTCTCACAAGTAGAGGTTTAAAGGAAGCCGATTTTGAAAAGATTG
CTGATTTCCTTGATAGAATCGTTTCAATCTCTCTTGAAATTCAAGGTCGTGTCGGTAAGA
NATTGGTTGATTTTTGTTGTTTTAATTNATTTTTNTTTTTTTNTTTTTNNTTTTTTNTTT
TTTT
Length of 3' end seq. 544
Connected seq. ID CHD465P
Connected seq. 
>CHD465P.Seq
ACACAAGTAATTATATAATATACATATACATATAAAATGGGTTCAGTATCAGGTAATACA
CCATTAAAAGAAGTTGATAATGAAATCTTTGAATTAATGAATAGAGAAAAAGATAGACAA
TTTAAAGGTTTAGAATTAATTGCCTCTGAAAATTTTACATCAAGAGCAGTTATGGAAGCA
TTAGGATCACATTTTACAAATAAATATGCAGAAGGTTATCCAGGTTCAAGATATTATGGT
GGTACAGAAGTTGTTGATGAATTAGAAACTCTTTGTCAAAAGAGAGCATTAAAAGCATTC
CGTTTGGATGAGAGTAAATGGGGTGTTAATGTTCAACCATACAGTGGTAGTCCAGCCAAT
TTCGCAGTTTACACAGCATTATTAAGACCACACGATCGTATAATGGGTTTAGATTTACCA
TCAGGTGGTCATTTAACTCATGGTTATCAAACCGATAAGAAGAAGATTTCAGCATCATCA
ATCTTTTTCGAATCAATGCCATATCAAATTGGTGCTGATGGTTTAATCGATTATCAACGT
TTAGAAGAGAATGCATT----------GTACGACATTGAATCAAAGATCAATTTCGCAGT
TTTCCCATCACTCCAAGGTGGATCACATGAAAATGTTATTGCTGGTGTTGCTGTTGCTCT
CAAAGAAGCCGACTCTCAAGAATTCAAAGAGTATGCCCTTCAAGTTAAAAAGAATGCCGC
TGCCATTGGTAATGCTCTCATGAACAAAGGTTATAAATTGGTTACAAATGGTACTGACAA
TCATTTAATTCTTTGGGATTTAAGACCAAAGGAACTCACTGGTAATAAATTTGAAAAAGC
TGCCGATATCGCTAATATCACCGTCAATAAAAATGCCGTCCATGGTGATACCAATGCAAT
TTCACCAGGTGGTATTCGTATTGGTTCATCTGCTCTCACAAGTAGAGGTTTAAAGGAAGC
CGATTTTGAAAAGATTGCTGATTTCCTTGATAGAATCGTTTCAATCTCTCTTGAAATTCA
AGGTCGTGTCGGTAAGANATTGGTTGATTTTTGTTGTTTTAATTNATTTTTNTTTTTTTN
TTTTTNNTTTTTTNTTTTTTT
Length of connected seq. 1091
Full length Seq ID -
Full length Seq. -
Length of full length seq. -