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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFF514
Library AF
(Link to library)
Clone ID AFF514
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16521-1
Original site URL
Representative seq. ID AFF514P
(Link to Original site)
Representative DNA sequence 
>AFF514 (AFF514Q) /CSM/AF/AFF5-A/AFF514Q.Seq.d/
CTAGGTAGAACGGTGGTATATTGTTTATTATTATTTGAAAAAGTCTTAATAAACATTATT
AAAGCAATTTAATAAAAGAAAATTTATAGAGGTAGAGCACTGAATGGTTCAACCTAAGGT
TATTATTTAAACTTCGAATACTATAAATAAATTGACCACTAGGCAGACTATGAGCGCTAA
GGTCCATAGGTCTAAAGGGAAACAGCCCAGATTGTAAGTTAATGTTCTTAAATAATAGTG
TAGTGTCAAAGGCATGGAGAAAGTGATGTTAATTGGAAAGTAGGATTGGAAGCGACCATC
TTTTAAAGAAAGCGTAAAAGCTCACCAATTTAAGGCGTTCTCTAGCCAACAATGTACAGG
AACTCAACATTATACAGAAACTACAAAATTATAACATTGGTAGTAAAATGTTCCGTATAA
TTGTGAAGTTAACTCAAGAAGAGATAATGGAGTTATCGGAATTAAGAATGCTGATATGAG
TAATGTTAATAAGGTTAAGACCTTATCACTGAAAATTAGAAGTTTCCTAATAAAAAGGTA
AGCTGATTAGGGTTACGCGATCGCTAAGATTAATATGACAATAAACTATCGATGCGCATA
TAGATTAATAATTCTAACGTTTTTATXXXXXXXXXXTTTTTATTGTAGTTTTAATTGAAC
ATGTGTATTTTAAATAATTATATTATACCTTACATTATAGATTATTTGAAATATAAGTTT
TAAAAATAGATTAAAACAAAATATCTGTAAAAAGATATTTTAAATAAAAAATCGTACTTA
AACCGACACTGGTTAATTGATAGAATATATTAAAGTGTAGAATGAATGATTCTGAAGGAA
CTCGGCAAAATAACTCCGTAACTGCGGGAGAAGGAGAGCCTTTGTTGTAAAAAATGAAGG
TCACAGTAAAGAAGGGGTGGCGACTGTTTAATAAAAACACAGGATTCTGCAAAATGGCAA
CGTTTAGTTACCCCAACCACCGTTGCCCGTAGAAAGGCTCTCCGTGCTAAAAAAGCTGCT
CGTCACACTAAAGCCAGCAATGAAGCCGCCGAATACGCTAAACTCGTTGCCCAAAGACAA
CAAGCTAAAGCCAAGAGATTCCGTCAAAGTTTCAAGCAAAAAAGTTGTTGNCCAAGTAAA
sequence update 2001. 6. 2
Translated Amino Acid sequence 
*verwyivyyylkks**tllkqfnkrkfieveh*mvqpkvii*tsntinklttrqtmsak
vhrskgkqprl*vnvlk**csvkgmekvmligk*dwkrpsfkesvkahqfkafssqqctg
tqhytettkl*hw**nvpyncevnsrrdngvigiknadmsnvnkvktlslkirsflikr*
ad*GYAIAKINMTINYRCAYRLIILTFL---

---FIVVLIEHVYFK*lyytlhyrlfei*vlkid*nkisvkryfk*kivlkptlvn**ni
lkcrmndsegtrqnnsvtagegepll*kmkvtvkkgwrlfnkntgfckmatfsypnhrcp
*kgspc*kscssh*sqq*srrir*trcpktts*sqeipskfqakkllxk*
Translated Amino Acid sequence (All Frames) 
Frame A:
lgrtvvyclllfekvliniikai**kkiyrgralngst*gyylnfeyyk*idh*adyer*
gp*v*retaqivs*cs*iiv*cqrhgesdvnwkvgleatif*rkrksspi*gvl*ptmyr
nstlyrnykiitlvvkcsv*l*s*lkkr*wsyrn*ec*ye*c**g*dliten*kfpnkkv
s*lglrdr*d*ydnklsmri*innsnvf---

---fll*f*lnmcilnnyiipyiidylkykf*k*iktkyl*kdilnkksylnrhwlidri
y*sve*milkelgkitp*lrekeslcckk*rsq*rrggdcliktqdsakwqrlvtpttva
rrkalrakkaarhtkasneaaeyaklvaqrqqakakrfrqsfkqkscxpsk

Frame B:
*verwyivyyylkks**tllkqfnkrkfieveh*mvqpkvii*tsntinklttrqtmsak
vhrskgkqprl*vnvlk**csvkgmekvmligk*dwkrpsfkesvkahqfkafssqqctg
tqhytettkl*hw**nvpyncevnsrrdngvigiknadmsnvnkvktlslkirsflikr*
ad*GYAIAKINMTINYRCAYRLIILTFL---

---fycsfn*tcvf*iiilyltl*ii*nisfknrlkqnickkif*iknrt*tdtg*liey
ikv*ne*f*rnsak*lrncgrrrafvvkneghskegvatv**khrilqngnv*lpqpplp
verlsvlkkllvtlkpamkppntlnslpkdnklkprdsvkvsskkvvxqv

Frame C:
r*nggilfiii*kslnkhy*snlikenl*r*stewfnlrllfklril*in*plgrl*alr
siglkgnspdcklmflnnsvvskawrk*c*lesrigsdhllkka*kltnlrrslannvqe
lniiqklqnynigskmfriivkltqeeimelselrmli*vmlirlrpyh*klevs**kgk
lirvtrslrli*q*tidahid**f*rfy---

---FIVVLIEHVYFK*lyytlhyrlfei*vlkid*nkisvkryfk*kivlkptlvn**ni
lkcrmndsegtrqnnsvtagegepll*kmkvtvkkgwrlfnkntgfckmatfsypnhrcp
*kgspc*kscssh*sqq*srrir*trcpktts*sqeipskfqakkllxk*
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFF514 (AFF514Q) /CSM/AF/AFF5-A/AFF514Q.Seq.d/                       1340   0.0
MIT-GENOME (MIT-GENOMEQ) /CSM/OTHERS/MIT-GENOMEQ.Seq.d/              1241   0.0
VHO432 (VHO432Q) /CSM/VH/VHO4-B/VHO432Q.Seq.d/                        954   0.0
VHL317 (VHL317Q) /CSM/VH/VHL3-A/VHL317Q.Seq.d/                        848   0.0
VSD302 (VSD302Q) /CSM/VS/VSD3-A/VSD302Q.Seq.d/                        700   0.0
CHF548 (CHF548Q) /CSM/CH/CHF5-B/CHF548Q.Seq.d/                        585   e-166
CHD527 (CHD527Q) /CSM/CH/CHD5-B/CHD527Q.Seq.d/                        579   e-164
CFD643 (CFD643Q) /CSM/CF/CFD6-B/CFD643Q.Seq.d/                        486   e-136
VHB176 (VHB176Q) /CSM/VH/VHB1-D/VHB176Q.Seq.d/                        383   e-105
VFE275 (VFE275Q) /CSM/VF/VFE2-D/VFE275Q.Seq.d/                        383   e-105
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U21880|U21880.1 Dictyostelium discoideum mitochondrion large subunit rRNA (rrn) gene, mitochondrial gene encoding mitochondrial rRNA, partial sequence.  1241   0.0    4
D16466|D16466.1 Dictyostelium discoideum mitochondrial DNA.      1241   0.0    4
AB000109|AB000109.1 Dictyostelium discoideum mitochondrial DNA, complete sequence.  1241   0.0    4
AF295546|AF295546.1 Malawimonas jakobiformis mitochondrial DNA, complete genome.    64   8e-50  8
Z67753|Z67753.1 Odontella sinensis complete chloroplast genome.    80   4e-26  9
U03732|U03732.1 Acanthamoeba castellanii Neff mitochondrion rRNA large subunit gene.    72   7e-23  4
AK105658|AK105658.1 Oryza sativa (japonica cultivar-group) cDNA clone:001-200-G09, full insert sequence.    58   5e-22  5
AF416765|AF416765.1 Ehrlichia chaffeensis strain Arkansas 23S ribosomal RNA and 5S ribosomal RNA genes, complete sequence.    56   6e-22  6
AK106482|AK106482.1 Oryza sativa (japonica cultivar-group) cDNA clone:002-104-F08, full insert sequence.    58   9e-22  5
AF268221|AF268221.2 Uncultured proteobacterium EBAC46D07 16S ribosomal RNA gene, partial sequence; tRNA-Ile gene, complete sequence; and 23S ribosomal RNA gene, partial sequence.    52   5e-21  6
dna update 2004. 4.24
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC151709_9(AC151709|pid:none) Medicago truncatula clone mth2-31g...    28   2.0
protein update 2008.11.19
PSORT 

 psg: 0.63  gvh: 0.53  alm: 0.19  top: 0.37  tms: 0.07  mit: 0.34  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.67  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 24.0 %: endoplasmic reticulum
	 20.0 %: mitochondrial
	 16.0 %: nuclear
	 16.0 %: cytoplasmic
	 12.0 %: Golgi
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system
	  4.0 %: peroxisomal

>> prediction for AFF514 is end
5' end seq. ID AFF514F
5' end seq. 
>AFF514F.Seq
CTAGGTAGAACGGTGGTATATTGTTTATTATTATTTGAAAAAGTCTTAATAAACATTATT
AAAGCAATTTAATAAAAGAAAATTTATAGAGGTAGAGCACTGAATGGTTCAACCTAAGGT
TATTATTTAAACTTCGAATACTATAAATAAATTGACCACTAGGCAGACTATGAGCGCTAA
GGTCCATAGGTCTAAAGGGAAACAGCCCAGATTGTAAGTTAATGTTCTTAAATAATAGTG
TAGTGTCAAAGGCATGGAGAAAGTGATGTTAATTGGAAAGTAGGATTGGAAGCGACCATC
TTTTAAAGAAAGCGTAAAAGCTCACCAATTTAAGGCGTTCTCTAGCCAACAATGTACAGG
AACTCAACATTATACAGAAACTACAAAATTATAACATTGGTAGTAAAATGTTCCGTATAA
TTGTGAAGTTAACTCAAGAAGAGATAATGGAGTTATCGGAATTAAGAATGCTGATATGAG
TAATGTTAATAAGGTTAAGACCTTATCACTGAAAATTAGAAGTTTCCTAATAAAAAGGTA
AGCTGATTAGGGTTACGCGATCGCTAAGATTAATATGACAATAAACTATCGATGCGCATA
TAGATTAATAATTCTAACGTTTTTAT----------
Length of 5' end seq. 626
3' end seq. ID AFF514Z
3' end seq. 
>AFF514Z.Seq
----------TTTTTATTGTAGTTTTAATTGAACATGTGTATTTTAAATAATTATATTAT
ACCTTACATTATAGATTATTTGAAATATAAGTTTTAAAAATAGATTAAAACAAAATATCT
GTAAAAAGATATTTTAAATAAAAAATCGTACTTAAACCGACACTGGTTAATTGATAGAAT
ATATTAAAGTGTAGAATGAATGATTCTGAAGGAACTCGGCAAAATAACTCCGTAACTGCG
GGAGAAGGAGAGCCTTTGTTGTAAAAAATGAAGGTCACAGTAAAGAAGGGGTGGCGACTG
TTTAATAAAAACACAGGATTCTGCAAAATGGCAACGTTTAGTTACCCCAACCACCGTTGC
CCGTAGAAAGGCTCTCCGTGCTAAAAAAGCTGCTCGTCACACTAAAGCCAGCAATGAAGC
CGCCGAATACGCTAAACTCGTTGCCCAAAGACAACAAGCTAAAGCCAAGAGATTCCGTCA
AAGTTTCAAGCAAAAAAGTTGTTGNCCAAGTAAA
Length of 3' end seq. 504
Connected seq. ID AFF514P
Connected seq. 
>AFF514P.Seq
CTAGGTAGAACGGTGGTATATTGTTTATTATTATTTGAAAAAGTCTTAATAAACATTATT
AAAGCAATTTAATAAAAGAAAATTTATAGAGGTAGAGCACTGAATGGTTCAACCTAAGGT
TATTATTTAAACTTCGAATACTATAAATAAATTGACCACTAGGCAGACTATGAGCGCTAA
GGTCCATAGGTCTAAAGGGAAACAGCCCAGATTGTAAGTTAATGTTCTTAAATAATAGTG
TAGTGTCAAAGGCATGGAGAAAGTGATGTTAATTGGAAAGTAGGATTGGAAGCGACCATC
TTTTAAAGAAAGCGTAAAAGCTCACCAATTTAAGGCGTTCTCTAGCCAACAATGTACAGG
AACTCAACATTATACAGAAACTACAAAATTATAACATTGGTAGTAAAATGTTCCGTATAA
TTGTGAAGTTAACTCAAGAAGAGATAATGGAGTTATCGGAATTAAGAATGCTGATATGAG
TAATGTTAATAAGGTTAAGACCTTATCACTGAAAATTAGAAGTTTCCTAATAAAAAGGTA
AGCTGATTAGGGTTACGCGATCGCTAAGATTAATATGACAATAAACTATCGATGCGCATA
TAGATTAATAATTCTAACGTTTTTAT----------TTTTTATTGTAGTTTTAATTGAAC
ATGTGTATTTTAAATAATTATATTATACCTTACATTATAGATTATTTGAAATATAAGTTT
TAAAAATAGATTAAAACAAAATATCTGTAAAAAGATATTTTAAATAAAAAATCGTACTTA
AACCGACACTGGTTAATTGATAGAATATATTAAAGTGTAGAATGAATGATTCTGAAGGAA
CTCGGCAAAATAACTCCGTAACTGCGGGAGAAGGAGAGCCTTTGTTGTAAAAAATGAAGG
TCACAGTAAAGAAGGGGTGGCGACTGTTTAATAAAAACACAGGATTCTGCAAAATGGCAA
CGTTTAGTTACCCCAACCACCGTTGCCCGTAGAAAGGCTCTCCGTGCTAAAAAAGCTGCT
CGTCACACTAAAGCCAGCAATGAAGCCGCCGAATACGCTAAACTCGTTGCCCAAAGACAA
CAAGCTAAAGCCAAGAGATTCCGTCAAAGTTTCAAGCAAAAAAGTTGTTGNCCAAGTAAA
Length of connected seq. 1130
Full length Seq ID -
Full length Seq. -
Length of full length seq. -