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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFF494
Library AF
(Link to library)
Clone ID AFF494
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U08743-1
Original site URL
Representative seq. ID AFF494P
(Link to Original site)
Representative DNA sequence 
>AFF494 (AFF494Q) /CSM/AF/AFF4-D/AFF494Q.Seq.d/
ATATTAAAAATCAAATAATTATGGGTAAAAATAAAAATTATAATAAAAATCAATTTAAAA
AATCAAAAACAAATAATGATACCACAGTAGCACAACAACAACAAACAATTGAAGAAAAAC
CAGATTTTAAAATTGATGGATCAATTTTAGAAGGTGGTGGTCAAATTTTAAGAAATTCAG
TTGCATTAGCATCATTATTTAATAAAGCCATTTCAATTGAAAAGATTAGGTATAATAGAG
ATCAACCAGGATTGAAAAATCAACATAAAGCTGGTATTGATTTAATGTCACGTTTATTTA
AAGCTCATTTAACAGGTTGTAGTGTTGGTTCATGTAAACTTTATTATCAACCAACACAAA
AGACAATTCAAGATGATGGTGTAATTGAAGCTGATACTAAAACAGCTGGTAGTATTTGTT
TGATGATTCAAGTTTCATTACCATGTTTAATCTTTGCACCACATTCCACAAAGATGGTAT
TGGGTGGTGGAACAAATTGTGATTTTGCACCAGCTGCAGACTATATTCAAAATGTTTTCC
TACCAATTGCAACAACTATGGGATTTAAATGTGAGATGAGTATTGATAAAAGAGGGTTXX
XXXXXXXXCTCACACAACCACTCTCGCCAATCACCATAGTGAATAAGGGTGAGGTGAATA
GAATTGTAATCAAGTCCTATCTCACGTCGCCTAGAATATCACCATTGGTAGCAGAGAGGA
TGAATAACACTGCAAAGAAATTAATAAAAAAGGATTTTAAAAAGGTGGATGTTGAAACAG
AGTTAATCGATGTGAGTAAATTTAGTTTTGGTGATGGTACATTCATTGAAATTAGGGCAT
ACACCGACCAAGGTTGTATATTTGGTGCAACTGGAAATGGTGCAATTGGTGTACCTGCTG
AAAAAGTCGCAGAAGATGCTGCAAATTCTTTACTCAAAGATTTGCAAGATGGTGGTTGTA
TGGATGAATACCTTCAAGATCAATTAATCATTTTCATGGCTTTAGCAAAAGGTAAAAGTC
AAATTAAAACTGGTCCAATCTCATTACATACTCGAACTTCAATTCATATAACATCTTTAA
TGACTGGTGCTATTTTTACAATTACACCATTAACTAATAATACTCAATCTGGTGAAGAAA
CAAATTTAATAACTTGTGAAGGTATTAGTTATTTTCCATCTGATTTAAATAATAATAATA
ATAATAGTAATAGTAATACTACTACTACTACAACTACCACTACAATTTCAACTACAACTA
TTATAACCAAAATAGTAAGNAAAAATAAAATA
sequence update 2001. 6. 2
Translated Amino Acid sequence 
IKNQIIMGKNKNYNKNQFKKSKTNNDTTVAQQQQTIEEKPDFKIDGSILEGGGQILRNSV
ALASLFNKAISIEKIRYNRDQPGLKNQHKAGIDLMSRLFKAHLTGCSVGSCKLYYQPTQK
TIQDDGVIEADTKTAGSICLMIQVSLPCLIFAPHSTKMVLGGGTNCDFAPAADYIQNVFL
PIATTMGFKCEMSIDKRG---

---LTQPLSPITIVNKGEVNRIVIKSYLTSPRISPLVAERMNNTAKKLIKKDFKKVDVET
ELIDVSKFSFGDGTFIEIRAYTDQGCIFGATGNGAIGVPAEKVAEDAANSLLKDLQDGGC
MDEYLQDQLIIFMALAKGKSQIKTGPISLHTRTSIHITSLMTGAIFTITPLTNNTQSGEE
TNLITCEGISYFPSDLNNNNNNSNSNTTTTTTTTTISTTTIITKIVXKNKI
Translated Amino Acid sequence (All Frames) 
Frame A:
ilkik*lwvkikiiikinlknqkqimipq*hnnnkqlkknqilklmdqf*kvvvkf*eiq
lh*hhylikpfqlkrlgiieinqd*kiniklvli*chvylkli*qvvvlvhvnfiinqhk
rqfkmmv*lklilkqlvvfv**fkfhyhv*slhhipqrwywvveqivilhqlqtifkmfs
yqlqqlwdlnvr*vlikeg---

---LTQPLSPITIVNKGEVNRIVIKSYLTSPRISPLVAERMNNTAKKLIKKDFKKVDVET
ELIDVSKFSFGDGTFIEIRAYTDQGCIFGATGNGAIGVPAEKVAEDAANSLLKDLQDGGC
MDEYLQDQLIIFMALAKGKSQIKTGPISLHTRTSIHITSLMTGAIFTITPLTNNTQSGEE
TNLITCEGISYFPSDLNNNNNNSNSNTTTTTTTTTISTTTIITKIVXKNKI

Frame B:
y*ksnnyg*k*kl**ksi*kiknk**yhssttttnn*rktrf*n*winfrrwwsnfkkfs
cisiii**shfn*kd*v**rstriekst*swy*fnvtfi*ssfnrl*cwfm*tllstntk
dnsr*wcn*s*y*nsw*ylfddssfitmfnlcttfhkdgigwwnkl*fctscrlyskcfp
tncnnygi*m*dey**krv---

---shnhsrqsp**irvr*iel*sspisrrleyhhw*qrg*itlqrn**krilkrwmlkq
s*sm*vnlvlvmvhslklghtptkvvylvqlemvqlvyllkksqkmlqilyskickmvvv
wmntfkin*sfswl*qkvkvklklvqshyilelqfi*hl**lvlflqlhh*liilnlvkk
qi**lvkvlvifhli*iiiiiivivillllqlplqfqlqll*pk**xkik

Frame C:
IKNQIIMGKNKNYNKNQFKKSKTNNDTTVAQQQQTIEEKPDFKIDGSILEGGGQILRNSV
ALASLFNKAISIEKIRYNRDQPGLKNQHKAGIDLMSRLFKAHLTGCSVGSCKLYYQPTQK
TIQDDGVIEADTKTAGSICLMIQVSLPCLIFAPHSTKMVLGGGTNCDFAPAADYIQNVFL
PIATTMGFKCEMSIDKRG---

---htttlanhhse*g*ge*ncnqvlshva*nitigsrede*hckeinkkgf*kggc*nr
vnrce*i*fw*wyih*n*gihrprlyiwcnwkwcnwctc*ksrrrcckfftqrfarwwly
g*ipsrsinhfhgfskr*ksn*nwsnlitysnfnsynifndwcyfynytin**ysiw*rn
kfnnl*ry*lfsi*fk*********yyyyynyhynfnynyynqnskxk*n
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFF494 (AFF494Q) /CSM/AF/AFF4-D/AFF494Q.Seq.d/                       2143   0.0
VFI760 (VFI760Q) /CSM/VF/VFI7-C/VFI760Q.Seq.d/                       1128   0.0
AFI290 (AFI290Q) /CSM/AF/AFI2-D/AFI290Q.Seq.d/                       1128   0.0
AFI119 (AFI119Q) /CSM/AF/AFI1-A/AFI119Q.Seq.d/                         46   0.001
CFI425 (CFI425Q) /CSM/CF/CFI4-B/CFI425Q.Seq.d/                         40   0.083
CFI303 (CFI303Q) /CSM/CF/CFI3-A/CFI303Q.Seq.d/                         40   0.083
VSB157 (VSB157Q) /CSM/VS/VSB1-C/VSB157Q.Seq.d/                         38   0.33
VHN565 (VHN565Q) /CSM/VH/VHN5-C/VHN565Q.Seq.d/                         38   0.33
VHI767 (VHI767Q) /CSM/VH/VHI7-C/VHI767Q.Seq.d/                         38   0.33
VHD748 (VHD748Q) /CSM/VH/VHD7-B/VHD748Q.Seq.d/                         38   0.33
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AF020279|AF020279.1 Dictyostelium discoideum R2037 gene, complete cds.  1128   0.0    2
AC116979|AC116979.3 Dictyostelium discoideum chromosome 2 map 6445720-6776760 strain AX4, complete sequence.  1128   0.0    1
AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.    34   4e-08  18
AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.    38   0.005  18
AC124419|AC124419.4 Mus musculus BAC clone RP24-319P11 from chromosome 18, complete sequence.    40   0.005  8
AC115612|AC115612.2 Dictyostelium discoideum chromosome 2 map 6245135-6357017 strain AX4, complete sequence.    34   0.008  11
AC144928|AC144928.9 Medicago truncatula clone mth1-8a18, WORKING DRAFT SEQUENCE, 38 unordered pieces.    42   0.038  6
AC116963|AC116963.2 Dictyostelium discoideum chromosome 2 map 4657875-4914984 strain AX4, complete sequence.    32   0.051  13
BX470266|BX470266.4 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone  DKEYP-9D4.    38   0.064  10
AC116309|AC116309.2 Homo sapiens chromosome 5 clone RP11-106J9, complete sequence.    38   0.081  10
dna update 2004. 4.24
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(O15746) RecName: Full=Probable RNA 3'-terminal phosphate cyclas...   697   0.0
BC079374_1(BC079374|pid:none) Rattus norvegicus RNA terminal pho...   246   1e-63
BC016519_1(BC016519|pid:none) Mus musculus RNA terminal phosphat...   245   2e-63
AK152129_1(AK152129|pid:none) Mus musculus bone marrow macrophag...   242   2e-62
(Q2HJ88) RecName: Full=RNA 3'-terminal phosphate cyclase;       ...   241   5e-62
BC090403_1(BC090403|pid:none) Mus musculus RNA terminal phosphat...   241   5e-62
(Q9D7H3) RecName: Full=RNA 3'-terminal phosphate cyclase;       ...   240   7e-62
AK151108_1(AK151108|pid:none) Mus musculus bone marrow macrophag...   239   1e-61
(Q4R3J0) RecName: Full=RNA 3'-terminal phosphate cyclase;       ...   239   1e-61
(O00442) RecName: Full=RNA 3'-terminal phosphate cyclase;       ...   237   7e-61
protein update 2009. 4.20
PSORT 

 psg: 0.41  gvh: 0.29  alm: 0.29  top: 0.70  tms: 0.07  mit: 0.28  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.80  pox: 0.30  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.02  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 1.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: nuclear
	 24.0 %: mitochondrial
	 24.0 %: cytoplasmic
	  8.0 %: plasma membrane
	  8.0 %: endoplasmic reticulum
	  4.0 %: vesicles of secretory system
	  4.0 %: peroxisomal

>> prediction for AFF494 is nuc
5' end seq. ID AFF494F
5' end seq. 
>AFF494F.Seq
ATATTAAAAATCAAATAATTATGGGTAAAAATAAAAATTATAATAAAAATCAATTTAAAA
AATCAAAAACAAATAATGATACCACAGTAGCACAACAACAACAAACAATTGAAGAAAAAC
CAGATTTTAAAATTGATGGATCAATTTTAGAAGGTGGTGGTCAAATTTTAAGAAATTCAG
TTGCATTAGCATCATTATTTAATAAAGCCATTTCAATTGAAAAGATTAGGTATAATAGAG
ATCAACCAGGATTGAAAAATCAACATAAAGCTGGTATTGATTTAATGTCACGTTTATTTA
AAGCTCATTTAACAGGTTGTAGTGTTGGTTCATGTAAACTTTATTATCAACCAACACAAA
AGACAATTCAAGATGATGGTGTAATTGAAGCTGATACTAAAACAGCTGGTAGTATTTGTT
TGATGATTCAAGTTTCATTACCATGTTTAATCTTTGCACCACATTCCACAAAGATGGTAT
TGGGTGGTGGAACAAATTGTGATTTTGCACCAGCTGCAGACTATATTCAAAATGTTTTCC
TACCAATTGCAACAACTATGGGATTTAAATGTGAGATGAGTATTGATAAAAGAGGGTT--
--------
Length of 5' end seq. 598
3' end seq. ID AFF494Z
3' end seq. 
>AFF494Z.Seq
----------CTCACACAACCACTCTCGCCAATCACCATAGTGAATAAGGGTGAGGTGAA
TAGAATTGTAATCAAGTCCTATCTCACGTCGCCTAGAATATCACCATTGGTAGCAGAGAG
GATGAATAACACTGCAAAGAAATTAATAAAAAAGGATTTTAAAAAGGTGGATGTTGAAAC
AGAGTTAATCGATGTGAGTAAATTTAGTTTTGGTGATGGTACATTCATTGAAATTAGGGC
ATACACCGACCAAGGTTGTATATTTGGTGCAACTGGAAATGGTGCAATTGGTGTACCTGC
TGAAAAAGTCGCAGAAGATGCTGCAAATTCTTTACTCAAAGATTTGCAAGATGGTGGTTG
TATGGATGAATACCTTCAAGATCAATTAATCATTTTCATGGCTTTAGCAAAAGGTAAAAG
TCAAATTAAAACTGGTCCAATCTCATTACATACTCGAACTTCAATTCATATAACATCTTT
AATGACTGGTGCTATTTTTACAATTACACCATTAACTAATAATACTCAATCTGGTGAAGA
AACAAATTTAATAACTTGTGAAGGTATTAGTTATTTTCCATCTGATTTAAATAATAATAA
TAATAATAGTAATAGTAATACTACTACTACTACAACTACCACTACAATTTCAACTACAAC
TATTATAACCAAAATAGTAAGNAAAAATAAAATA
Length of 3' end seq. 684
Connected seq. ID AFF494P
Connected seq. 
>AFF494P.Seq
ATATTAAAAATCAAATAATTATGGGTAAAAATAAAAATTATAATAAAAATCAATTTAAAA
AATCAAAAACAAATAATGATACCACAGTAGCACAACAACAACAAACAATTGAAGAAAAAC
CAGATTTTAAAATTGATGGATCAATTTTAGAAGGTGGTGGTCAAATTTTAAGAAATTCAG
TTGCATTAGCATCATTATTTAATAAAGCCATTTCAATTGAAAAGATTAGGTATAATAGAG
ATCAACCAGGATTGAAAAATCAACATAAAGCTGGTATTGATTTAATGTCACGTTTATTTA
AAGCTCATTTAACAGGTTGTAGTGTTGGTTCATGTAAACTTTATTATCAACCAACACAAA
AGACAATTCAAGATGATGGTGTAATTGAAGCTGATACTAAAACAGCTGGTAGTATTTGTT
TGATGATTCAAGTTTCATTACCATGTTTAATCTTTGCACCACATTCCACAAAGATGGTAT
TGGGTGGTGGAACAAATTGTGATTTTGCACCAGCTGCAGACTATATTCAAAATGTTTTCC
TACCAATTGCAACAACTATGGGATTTAAATGTGAGATGAGTATTGATAAAAGAGGGTT--
--------CTCACACAACCACTCTCGCCAATCACCATAGTGAATAAGGGTGAGGTGAATA
GAATTGTAATCAAGTCCTATCTCACGTCGCCTAGAATATCACCATTGGTAGCAGAGAGGA
TGAATAACACTGCAAAGAAATTAATAAAAAAGGATTTTAAAAAGGTGGATGTTGAAACAG
AGTTAATCGATGTGAGTAAATTTAGTTTTGGTGATGGTACATTCATTGAAATTAGGGCAT
ACACCGACCAAGGTTGTATATTTGGTGCAACTGGAAATGGTGCAATTGGTGTACCTGCTG
AAAAAGTCGCAGAAGATGCTGCAAATTCTTTACTCAAAGATTTGCAAGATGGTGGTTGTA
TGGATGAATACCTTCAAGATCAATTAATCATTTTCATGGCTTTAGCAAAAGGTAAAAGTC
AAATTAAAACTGGTCCAATCTCATTACATACTCGAACTTCAATTCATATAACATCTTTAA
TGACTGGTGCTATTTTTACAATTACACCATTAACTAATAATACTCAATCTGGTGAAGAAA
CAAATTTAATAACTTGTGAAGGTATTAGTTATTTTCCATCTGATTTAAATAATAATAATA
ATAATAGTAATAGTAATACTACTACTACTACAACTACCACTACAATTTCAACTACAACTA
TTATAACCAAAATAGTAAGNAAAAATAAAATA
Length of connected seq. 1282
Full length Seq ID -
Full length Seq. -
Length of full length seq. -