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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFF490
Library AF
(Link to library)
Clone ID AFF490
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16512-1
Original site URL
Representative seq. ID AFF490P
(Link to Original site)
Representative DNA sequence 
>AFF490 (AFF490Q) /CSM/AF/AFF4-D/AFF490Q.Seq.d/
TAAGCATAAACGGTGAATACCTNGACTCCTAAATCGATGAAGACCGTAGCAAACTGCGAT
AATTCACTTGAATTGCAGCCTACTGGGATAGTTGAAATGTTGAACGCACATGATGACATC
GGTCCTTTCGGATTAGGTGTTATACTTGGGTGAGAGTGGTCATTTATTAATAATACATCC
ACAAAGAATGAAAATATTATTATTTGTTACATTAATTGCATTGGCATTTGTTGCCTTATG
CTCAGCAGAAGGAAATGTTGTTGTTTTATCACCAGACAACTTTGATACAGNTGCTGATGG
TTCAAAAACTGTATTTGTCAAATTCTATGCACCATGGXXXXXXXXXXAGAAAGCACCATC
AAATGTTGTTGATTTATCACCATCAAATTTTGATTCAGTTGTTTTAGATAAATCAAAGAA
TGTTTTAGTTGAATTCTATGCACCATGGTGTGGTCATTGCAAAAAGTTAATGCCAGATTA
TGAAATCCTTGGTAACACCTACGCCAATGAAAAAGATGTTGTCATCGCCAAAATTGATTG
CGATGCTGCTGATAACAAAGCCATCTGCTCCAAATATGGTGTCACTGGTTTCCCAACACT
CAAATGGTTCGGTAAACAAAGCAAAGACGGTGAAAAATATGAACAAGGTCGTGATCTTGA
TACTTTCATCAACTACATCAACAAACAAGCTGGTGTTAACCGTGTCAAAGGTGGTAAACT
CGCTGTTGGTGCTGGTCGTGTTGAACAACTCGATACTATTGCCACTGAATTCATCGCTGC
CGCTGCTGAAGTCCGTAAGGAACTCGTCAAGAAAGCTCAAACCGTCGTTGATTCTCTCCC
AGAAGAATTAAGAACTGAAGGTTCATACTACGTCAAAGTCATGAAAACCATTGCTGAAAA
ATCAATCGATTTTGTAGCCACTGAAATCGCTCGTATCACCAAATTAGTCTCTGGTTCAAT
GTCTGGAAAGAAAGCTGATGAATTTGCCAAGAAACTCAATATTTTAGAATCTTTCAAATC
AAAATAAATTAAATTTAATTTAGAAAATAAAAAATAAAATAAAAGAAAAAAAATA
sequence update 2001. 6. 2
Translated Amino Acid sequence 
khkr*ipxllnr*rp*qtaiihlncsllg*lkc*thmmtsvlsd*VLYLGESGHLLIIHP
QRMKILLFVTLIALAFVALCSAEGNVVVLSPDNFDTXADGSKTVFVKFYAPW---

---KAPSNVVDLSPSNFDSVVLDKSKNVLVEFYAPWCGHCKKLMPDYEILGNTYANEKDV
VIAKIDCDAADNKAICSKYGVTGFPTLKWFGKQSKDGEKYEQGRDLDTFINYINKQAGVN
RVKGGKLAVGAGRVEQLDTIATEFIAAAAEVRKELVKKAQTVVDSLPEELRTEGSYYVKV
MKTIAEKSIDFVATEIARITKLVSGSMSGKKADEFAKKLNILESFKSK*ikfnlenkk*n
krkki
Translated Amino Acid sequence (All Frames) 
Frame A:
*a*tvntxtpksmktvancdnslelqptgivemlnahddigpfglgvilg*ewsfinnts
tkneniiicyincigicclmlsrrkcccfitrql*yxc*wfkncicqilctm---

---rkhhqmlliyhhqiliqlf*inqrmf*lnsmhhgvviaks*cqimkslvtptpmkkm
lsspkliamllitkpsapnmvslvsqhsngsvnkaktvknmnkvvililssttstnklvl
tvskvvnsllvlvvlnnsillplnsslpllksvrnssrklkpslilsqkn*elkvhttsk
s*kpllknqsil*plkslvspn*slvqclerklmnlprnsif*nlsnqnklnli*kiknk
ikekk

Frame B:
khkr*ipxllnr*rp*qtaiihlncsllg*lkc*thmmtsvlsd*VLYLGESGHLLIIHP
QRMKILLFVTLIALAFVALCSAEGNVVVLSPDNFDTXADGSKTVFVKFYAPW---

---estikcc*fitikf*fscfr*ikecfs*ilctmvwslqkvnarl*npw*hlrq*krc
chrqn*lrcc**qshllqiwchwfpntqmvr*tkqrr*ki*trs*s*yfhqlhqqtswc*
pcqrw*trcwcwsc*ttryych*ihrcrc*sp*gtrqessnrr*fsprrikn*rfilrqs
henhc*kinrfcsh*nrsyhqislwfnvwkes**icqetqyfrifqikin*i*frk*kik
*kkkn

Frame C:
singeylds*idedrsklr*ft*iaaywds*nvert**hrsfrircytwvrvviy**yih
ke*kyyyllh*lhwhllpyaqqkemllfyhqttliqxlmvqklylsnsmhh---

---KAPSNVVDLSPSNFDSVVLDKSKNVLVEFYAPWCGHCKKLMPDYEILGNTYANEKDV
VIAKIDCDAADNKAICSKYGVTGFPTLKWFGKQSKDGEKYEQGRDLDTFINYINKQAGVN
RVKGGKLAVGAGRVEQLDTIATEFIAAAAEVRKELVKKAQTVVDSLPEELRTEGSYYVKV
MKTIAEKSIDFVATEIARITKLVSGSMSGKKADEFAKKLNILESFKSK*ikfnlenkk*n
krkki
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFF490 (AFF490Q) /CSM/AF/AFF4-D/AFF490Q.Seq.d/                       1941   0.0
AFN801 (AFN801Q) /CSM/AF/AFN8-A/AFN801Q.Seq.d/                       1324   0.0
VFO821 (VFO821Q) /CSM/VF/VFO8-A/VFO821Q.Seq.d/                       1316   0.0
VFO241 (VFO241Q) /CSM/VF/VFO2-B/VFO241Q.Seq.d/                       1316   0.0
VFN359 (VFN359Q) /CSM/VF/VFN3-C/VFN359Q.Seq.d/                       1316   0.0
VFN233 (VFN233Q) /CSM/VF/VFN2-B/VFN233Q.Seq.d/                       1316   0.0
VFM578 (VFM578Q) /CSM/VF/VFM5-D/VFM578Q.Seq.d/                       1316   0.0
VFM537 (VFM537Q) /CSM/VF/VFM5-B/VFM537Q.Seq.d/                       1316   0.0
VFL481 (VFL481Q) /CSM/VF/VFL4-D/VFL481Q.Seq.d/                       1316   0.0
VFL370 (VFL370Q) /CSM/VF/VFL3-C/VFL370Q.Seq.d/                       1316   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116979|AC116979.3 Dictyostelium discoideum chromosome 2 map 6445720-6776760 strain AX4, complete sequence.  1316   0.0    11
AF019112|AF019112.1 Dictyostelium discoideum protein disulfide isomerase (dis) mRNA, complete cds.  1308   0.0    2
X00601|X00601.1 Dictyostelium discoideum ribosomal RNA gene.      313   3e-81  1
AY171066|AY171066.1 Dictyostelium discoideum extrachromosomal palindromic ribosomal RNA gene sequence, central segment.   313   3e-81  1
V00192|V00192.1 Dictyostelium discoideum 5.8S ribosomal RNA.      297   2e-76  1
AF219102|AF219102.1 Dictyostelium giganteum 18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence.   270   4e-68  1
AF219103|AF219103.1 Dictyostelium purpureum 18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence.   270   4e-68  1
AF219101|AF219101.1 Dictyostelium dimigraformum 18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence.   268   2e-67  1
AY330646|AY330646.1 Dictyostelium clavatum 18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence.   262   1e-65  1
AF219107|AF219107.1 Polysphondylium violaceum 18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence.   262   1e-65  1
dna update 2004. 4.24
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q86IA3) RecName: Full=Protein disulfide-isomerase 1;          S...   417   e-115
AF019112_1(AF019112|pid:none) Dictyostelium discoideum protein d...   415   e-114
EF086125_1(EF086125|pid:none) Picea sitchensis clone WS0285_I21 ...   189   1e-46
(O22263) RecName: Full=Probable protein disulfide-isomerase A6; ...   188   2e-46
AK317714_1(AK317714|pid:none) Arabidopsis thaliana AT2G47470 mRN...   188   2e-46
EU284948_1(EU284948|pid:none) TSA: Elaeis guineensis EgEfMPOB001...   186   9e-46
AC087425_4(AC087425|pid:none) Oryza sativa (japonica cultivar-gr...   186   9e-46
T03644(T03644) probable protein disulfide-isomerase (EC 5.3.4.1)...   185   2e-45
DQ222488_1(DQ222488|pid:none) Solanum tuberosum clone 098B03 dis...   184   3e-45
AE017341_67(AE017341|pid:none) Cryptococcus neoformans var. neof...   184   4e-45
protein update 2009. 4.20
PSORT 

 psg: 0.75  gvh: 0.77  alm: 0.16  top: 0.20  tms: 0.07  mit: 0.21  mip: 0.04
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.25  leu: 0.25  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 24.0 %: cytoplasmic
	 20.0 %: nuclear
	 16.0 %: endoplasmic reticulum
	 12.0 %: Golgi
	 12.0 %: mitochondrial
	  4.0 %: extracellular, including cell wall
	  4.0 %: peroxisomal
	  4.0 %: vacuolar
	  4.0 %: plasma membrane

>> prediction for AFF490 is cyt
5' end seq. ID AFF490F
5' end seq. 
>AFF490F.Seq
TAAGCATAAACGGTGAATACCTNGACTCCTAAATCGATGAAGACCGTAGCAAACTGCGAT
AATTCACTTGAATTGCAGCCTACTGGGATAGTTGAAATGTTGAACGCACATGATGACATC
GGTCCTTTCGGATTAGGTGTTATACTTGGGTGAGAGTGGTCATTTATTAATAATACATCC
ACAAAGAATGAAAATATTATTATTTGTTACATTAATTGCATTGGCATTTGTTGCCTTATG
CTCAGCAGAAGGAAATGTTGTTGTTTTATCACCAGACAACTTTGATACAGNTGCTGATGG
TTCAAAAACTGTATTTGTCAAATTCTATGCACCATGG----------
Length of 5' end seq. 337
3' end seq. ID AFF490Z
3' end seq. 
>AFF490Z.Seq
----------AGAAAGCACCATCAAATGTTGTTGATTTATCACCATCAAATTTTGATTCA
GTTGTTTTAGATAAATCAAAGAATGTTTTAGTTGAATTCTATGCACCATGGTGTGGTCAT
TGCAAAAAGTTAATGCCAGATTATGAAATCCTTGGTAACACCTACGCCAATGAAAAAGAT
GTTGTCATCGCCAAAATTGATTGCGATGCTGCTGATAACAAAGCCATCTGCTCCAAATAT
GGTGTCACTGGTTTCCCAACACTCAAATGGTTCGGTAAACAAAGCAAAGACGGTGAAAAA
TATGAACAAGGTCGTGATCTTGATACTTTCATCAACTACATCAACAAACAAGCTGGTGTT
AACCGTGTCAAAGGTGGTAAACTCGCTGTTGGTGCTGGTCGTGTTGAACAACTCGATACT
ATTGCCACTGAATTCATCGCTGCCGCTGCTGAAGTCCGTAAGGAACTCGTCAAGAAAGCT
CAAACCGTCGTTGATTCTCTCCCAGAAGAATTAAGAACTGAAGGTTCATACTACGTCAAA
GTCATGAAAACCATTGCTGAAAAATCAATCGATTTTGTAGCCACTGAAATCGCTCGTATC
ACCAAATTAGTCTCTGGTTCAATGTCTGGAAAGAAAGCTGATGAATTTGCCAAGAAACTC
AATATTTTAGAATCTTTCAAATCAAAATAAATTAAATTTAATTTAGAAAATAAAAAATAA
AATAAAAGAAAAAAAATA
Length of 3' end seq. 728
Connected seq. ID AFF490P
Connected seq. 
>AFF490P.Seq
TAAGCATAAACGGTGAATACCTNGACTCCTAAATCGATGAAGACCGTAGCAAACTGCGAT
AATTCACTTGAATTGCAGCCTACTGGGATAGTTGAAATGTTGAACGCACATGATGACATC
GGTCCTTTCGGATTAGGTGTTATACTTGGGTGAGAGTGGTCATTTATTAATAATACATCC
ACAAAGAATGAAAATATTATTATTTGTTACATTAATTGCATTGGCATTTGTTGCCTTATG
CTCAGCAGAAGGAAATGTTGTTGTTTTATCACCAGACAACTTTGATACAGNTGCTGATGG
TTCAAAAACTGTATTTGTCAAATTCTATGCACCATGG----------AGAAAGCACCATC
AAATGTTGTTGATTTATCACCATCAAATTTTGATTCAGTTGTTTTAGATAAATCAAAGAA
TGTTTTAGTTGAATTCTATGCACCATGGTGTGGTCATTGCAAAAAGTTAATGCCAGATTA
TGAAATCCTTGGTAACACCTACGCCAATGAAAAAGATGTTGTCATCGCCAAAATTGATTG
CGATGCTGCTGATAACAAAGCCATCTGCTCCAAATATGGTGTCACTGGTTTCCCAACACT
CAAATGGTTCGGTAAACAAAGCAAAGACGGTGAAAAATATGAACAAGGTCGTGATCTTGA
TACTTTCATCAACTACATCAACAAACAAGCTGGTGTTAACCGTGTCAAAGGTGGTAAACT
CGCTGTTGGTGCTGGTCGTGTTGAACAACTCGATACTATTGCCACTGAATTCATCGCTGC
CGCTGCTGAAGTCCGTAAGGAACTCGTCAAGAAAGCTCAAACCGTCGTTGATTCTCTCCC
AGAAGAATTAAGAACTGAAGGTTCATACTACGTCAAAGTCATGAAAACCATTGCTGAAAA
ATCAATCGATTTTGTAGCCACTGAAATCGCTCGTATCACCAAATTAGTCTCTGGTTCAAT
GTCTGGAAAGAAAGCTGATGAATTTGCCAAGAAACTCAATATTTTAGAATCTTTCAAATC
AAAATAAATTAAATTTAATTTAGAAAATAAAAAATAAAATAAAAGAAAAAAAATA
Length of connected seq. 1065
Full length Seq ID -
Full length Seq. -
Length of full length seq. -