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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFF485
Library AF
(Link to library)
Clone ID AFF485
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16331-1
Original site URL
Representative seq. ID AFF485P
(Link to Original site)
Representative DNA sequence 
>AFF485 (AFF485Q) /CSM/AF/AFF4-D/AFF485Q.Seq.d/
TAGAATCCATTAGAAAGATTGAACAATTGATTTTGGATGAAAATAATCAACTCTGTAAAT
CATTTGACAATGTTGATTTAATTGATATGAATGTACTATTATTCAGAATCAATGAAGAGG
AGAAACAAGTCATTGGTCATGGTGCTTATCATGTCCAAAATCCAGTTGGTGAATTATCAT
TCTGTGGTTTACAAGGTTTCGTAACTGAATTAAATCAAATTGCAAAGCATAATGATTTGG
GTAATCAATTATGTGGGAATCTTAGAAAAGGCAATTGGGCAATCGATTATATCGTCAATA
GATTGGATCAAAGAGTAGCCTTGAAATCAGTGAAACAATGGTTAACTCAATGTTTTGATC
AATTGAAACAATTACCAAGACATTTAATACCAATGTATTTCTATCAAATCATTATGACAA
CCTATCGTTTCGCCACAAATAAATCCATTGAAAAGATGGGTAATTCATTCGTAAAGGAGA
GTGATTCTTGGTTTTCACGTGCATTGGCTCTCACGAGTATACAATTCTTTGGTGTTTCAA
CACCATTAATTTCAAATNCAAATGTATTAGAAGGTGTTAAAGATAGAGAAGXXXXXXXXX
XTAACTTATCAAGATTGGTCAAAATTAATTGAAACAAATTTTGAAAAATATTATTATGTT
CCATCAAGCGGTGATGATAAGAAATATATTATCAATTCAAATTACGTTCATCGTAGATTC
ATTTATAAAGATGTAGTTGGTAGTGCAAACGTTTTCTCTGATTACCAATTAAGACCAAAT
ATTTGTGTTGCAATGTCATTTGCACCAAAGTTATTCAATGATCAACATGCAAATAAATGT
ATTGATATCATTAGAAATTCATTGGTTGGTCCAATCGGTATGAAAACTCTTGATCCAAAT
GATCCAAATTATCATGGTAACTATGATAATTCATCAGATTCTGGTTATAAACCAACATCA
ANAGGTTTCAATTATCATAATGGTCCTGAATGGGTTTGGTGTTATGGTTTCTTTTTGGAA
TCAATTGTACAATTTAAACAGTTCCCTTCGATTTCAACAAAGAATCATTTAATCAATTCA
TTATTAATTAAACATAAACTTTACATTCAAAATGATAAACGTTTCTCATTACCAGAGTTA
ACTAATCGTGAAGGTAGTTTTTGTAAANATAGTTGTGATTCTCAAGCTTGGTCAATTGCA
ACAATTTTAGCTTCACCTTATCAATTATCAATTACTAAAAATTAAATATTCATTTTTTTA
TANATATGTATATATTAAAAAAAAAAGCCAACTTTTTTAAACAC
sequence update 2001. 6. 2
Translated Amino Acid sequence 
ESIRKIEQLILDENNQLCKSFDNVDLIDMNVLLFRINEEEKQVIGHGAYHVQNPVGELSF
CGLQGFVTELNQIAKHNDLGNQLCGNLRKGNWAIDYIVNRLDQRVALKSVKQWLTQCFDQ
LKQLPRHLIPMYFYQIIMTTYRFATNKSIEKMGNSFVKESDSWFSRALALTSIQFFGVST
PLISNXNVLEGVKDRE---

---TYQDWSKLIETNFEKYYYVPSSGDDKKYIINSNYVHRRFIYKDVVGSANVFSDYQLR
PNICVAMSFAPKLFNDQHANKCIDIIRNSLVGPIGMKTLDPNDPNYHGNYDNSSDSGYKP
TSXGFNYHNGPEWVWCYGFFLESIVQFKQFPSISTKNHLINSLLIKHKLYIQNDKRFSLP
ELTNREGSFCKXSCDSQAWSIATILASPYQLSITKN*ififlxiciy*kkkptfln
Translated Amino Acid sequence (All Frames) 
Frame A:
*nplerlnn*fwmkiinsvnhltmli*li*myyysesmkrrnkslvmvlimskiqlvnyh
svvykvs*ln*iklqsimiwvinyvgilekaigqsiissidwike*p*nq*nng*lnvli
n*nnyqdi*yqcisiksl*qpivspqinplkrwvihs*rrvilgfhvhwlsrvynslvfq
hh*fqxqmy*kvlkiek---

---*likigqn*lkqilkniimfhqavmirnilsiqitfivdsfikm*lvvqtfslitn*
dqifvlqchlhqsysminmqinvlisleihwlvqsv*klliqmiqiimvtmiihqilvin
qhqxvsiiimvlngfgvmvsfwnqlynlnsslrfqqrii*sihy*lninftfkminvshy
qs*livkvvfvxivvilklgqlqqf*lhlinyqllkikysffyxyvyikkksqlf*t

Frame B:
rih*kd*tidfg*k*stl*ii*qc*fn*yectiiqnq*rgetshwswclscpkssw*iii
lwftrfrn*iksncka**fg*simwes*krqlgnrlyrq*igskssleisetmvnsmf*s
ietitktfntnvflsnhydnlsfrhk*ih*kdg*firkge*flvftcigsheytilwcfn
tinfkxkcirrc*r*r---

---nlsrlvkin*nkf*killcsikr***eiyyqfklrss*ihl*rcsw*ckrfl*lpik
tkylccnvictkviq*stck*my*yh*kfigwsnryens*sk*sklsw*l**firfwl*t
nixrfqls*ws*mglvlwflfgincti*tvpfdfnkesfnqfiin*t*tlhsk**tflit
rvn*s*r*fl*x*l*fsslvncnnfsftlsiiny*klnihffixmyilkkkanffkh

Frame C:
ESIRKIEQLILDENNQLCKSFDNVDLIDMNVLLFRINEEEKQVIGHGAYHVQNPVGELSF
CGLQGFVTELNQIAKHNDLGNQLCGNLRKGNWAIDYIVNRLDQRVALKSVKQWLTQCFDQ
LKQLPRHLIPMYFYQIIMTTYRFATNKSIEKMGNSFVKESDSWFSRALALTSIQFFGVST
PLISNXNVLEGVKDRE---

---TYQDWSKLIETNFEKYYYVPSSGDDKKYIINSNYVHRRFIYKDVVGSANVFSDYQLR
PNICVAMSFAPKLFNDQHANKCIDIIRNSLVGPIGMKTLDPNDPNYHGNYDNSSDSGYKP
TSXGFNYHNGPEWVWCYGFFLESIVQFKQFPSISTKNHLINSLLIKHKLYIQNDKRFSLP
ELTNREGSFCKXSCDSQAWSIATILASPYQLSITKN*ififlxiciy*kkkptfln
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFF485 (AFF485Q) /CSM/AF/AFF4-D/AFF485Q.Seq.d/                       2405   0.0
VHD429 (VHD429Q) /CSM/VH/VHD4-B/VHD429Q.Seq.d/                       1267   0.0
CHM267 (CHM267Q) /CSM/CH/CHM2-C/CHM267Q.Seq.d/                       1265   0.0
SHD317 (SHD317Q) /CSM/SH/SHD3-A/SHD317Q.Seq.d/                       1255   0.0
AHN666 (AHN666Q) /CSM/AH/AHN6-C/AHN666Q.Seq.d/                       1255   0.0
AHD862 (AHD862Q) /CSM/AH/AHD8-C/AHD862Q.Seq.d/                       1253   0.0
CHE549 (CHE549Q) /CSM/CH/CHE5-C/CHE549Q.Seq.d/                       1249   0.0
SHL636 (SHL636Q) /CSM/SH/SHL6-B/SHL636Q.Seq.d/                       1243   0.0
AHK567 (AHK567Q) /CSM/AH/AHK5-C/AHK567Q.Seq.d/                       1243   0.0
AHB484 (AHB484Q) /CSM/AH/AHB4-D/AHB484Q.Seq.d/                       1243   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116963|AC116963.2 Dictyostelium discoideum chromosome 2 map 4657875-4914984 strain AX4, complete sequence.    44   3e-10  22
AZ689714|AZ689714.1 ENTJV02TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    48   5e-08  3
BH160738|BH160738.1 ENTQS94TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    48   5e-08  3
BH163073|BH163073.1 ENTTA93TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    48   6e-08  3
AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.    34   2e-07  24
BX842642|BX842642.1 Mycoplasma mycoides subsp. mycoides SC genomic DNA, complete  sequence; segment 1/4.    38   3e-05  16
AZ680113|AZ680113.1 ENTGV48TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    48   2e-04  2
AZ547389|AZ547389.1 ENTGR73TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    48   6e-04  2
AC116982|AC116982.2 Dictyostelium discoideum chromosome 2 map 3622643-3879522 strain AX4, complete sequence.    32   0.002  19
AE014827|AE014827.1 Plasmodium falciparum 3D7 chromosome 14 section 12 of 13 of the complete sequence.    32   0.002  17
dna update 2004. 4.24
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

M85168_1(M85168|pid:none) Human glycogen debranching enzyme mRNA...   192   3e-47
U84011_1(U84011|pid:none) Human glycogen debranching enzyme isof...   192   3e-47
(P35573) RecName: Full=Glycogen debranching enzyme; AltName: Ful...   190   8e-47
BC167247_1(BC167247|pid:none) Synthetic construct Mus musculus c...   190   8e-47
AK028914_1(AK028914|pid:none) Mus musculus 10 days neonate skin ...   190   8e-47
EF660736_1(EF660736|pid:none) Equus caballus amylo-1, 6-glucosid...   188   3e-46
EF660736_5(EF660736|pid:none) Equus caballus amylo-1, 6-glucosid...   188   3e-46
AE016818_70(AE016818|pid:none) Ashbya gossypii (= Eremothecium g...   180   1e-43
BC091979_1(BC091979|pid:none) Danio rerio im:7145503, mRNA (cDNA...   179   2e-43
FM992691_453(FM992691|pid:none) Candida dubliniensis CD36 chromo...   177   5e-43
protein update 2009. 4.20
PSORT 

 psg: 0.52  gvh: 0.35  alm: 0.37  top: 0.53  tms: 0.00  mit: 0.19  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 60.0 %: cytoplasmic
	 28.0 %: nuclear
	  8.0 %: mitochondrial
	  4.0 %: cytoskeletal

>> prediction for AFF485 is cyt
5' end seq. ID AFF485F
5' end seq. 
>AFF485F.Seq
TAGAATCCATTAGAAAGATTGAACAATTGATTTTGGATGAAAATAATCAACTCTGTAAAT
CATTTGACAATGTTGATTTAATTGATATGAATGTACTATTATTCAGAATCAATGAAGAGG
AGAAACAAGTCATTGGTCATGGTGCTTATCATGTCCAAAATCCAGTTGGTGAATTATCAT
TCTGTGGTTTACAAGGTTTCGTAACTGAATTAAATCAAATTGCAAAGCATAATGATTTGG
GTAATCAATTATGTGGGAATCTTAGAAAAGGCAATTGGGCAATCGATTATATCGTCAATA
GATTGGATCAAAGAGTAGCCTTGAAATCAGTGAAACAATGGTTAACTCAATGTTTTGATC
AATTGAAACAATTACCAAGACATTTAATACCAATGTATTTCTATCAAATCATTATGACAA
CCTATCGTTTCGCCACAAATAAATCCATTGAAAAGATGGGTAATTCATTCGTAAAGGAGA
GTGATTCTTGGTTTTCACGTGCATTGGCTCTCACGAGTATACAATTCTTTGGTGTTTCAA
CACCATTAATTTCAAATNCAAATGTATTAGAAGGTGTTAAAGATAGAGAAG---------
-
Length of 5' end seq. 591
3' end seq. ID AFF485Z
3' end seq. 
>AFF485Z.Seq
----------TAACTTATCAAGATTGGTCAAAATTAATTGAAACAAATTTTGAAAAATAT
TATTATGTTCCATCAAGCGGTGATGATAAGAAATATATTATCAATTCAAATTACGTTCAT
CGTAGATTCATTTATAAAGATGTAGTTGGTAGTGCAAACGTTTTCTCTGATTACCAATTA
AGACCAAATATTTGTGTTGCAATGTCATTTGCACCAAAGTTATTCAATGATCAACATGCA
AATAAATGTATTGATATCATTAGAAATTCATTGGTTGGTCCAATCGGTATGAAAACTCTT
GATCCAAATGATCCAAATTATCATGGTAACTATGATAATTCATCAGATTCTGGTTATAAA
CCAACATCAANAGGTTTCAATTATCATAATGGTCCTGAATGGGTTTGGTGTTATGGTTTC
TTTTTGGAATCAATTGTACAATTTAAACAGTTCCCTTCGATTTCAACAAAGAATCATTTA
ATCAATTCATTATTAATTAAACATAAACTTTACATTCAAAATGATAAACGTTTCTCATTA
CCAGAGTTAACTAATCGTGAAGGTAGTTTTTGTAAANATAGTTGTGATTCTCAAGCTTGG
TCAATTGCAACAATTTTAGCTTCACCTTATCAATTATCAATTACTAAAAATTAAATATTC
ATTTTTTTATANATATGTATATATTAAAAAAAAAAGCCAACTTTTTTAAACAC
Length of 3' end seq. 703
Connected seq. ID AFF485P
Connected seq. 
>AFF485P.Seq
TAGAATCCATTAGAAAGATTGAACAATTGATTTTGGATGAAAATAATCAACTCTGTAAAT
CATTTGACAATGTTGATTTAATTGATATGAATGTACTATTATTCAGAATCAATGAAGAGG
AGAAACAAGTCATTGGTCATGGTGCTTATCATGTCCAAAATCCAGTTGGTGAATTATCAT
TCTGTGGTTTACAAGGTTTCGTAACTGAATTAAATCAAATTGCAAAGCATAATGATTTGG
GTAATCAATTATGTGGGAATCTTAGAAAAGGCAATTGGGCAATCGATTATATCGTCAATA
GATTGGATCAAAGAGTAGCCTTGAAATCAGTGAAACAATGGTTAACTCAATGTTTTGATC
AATTGAAACAATTACCAAGACATTTAATACCAATGTATTTCTATCAAATCATTATGACAA
CCTATCGTTTCGCCACAAATAAATCCATTGAAAAGATGGGTAATTCATTCGTAAAGGAGA
GTGATTCTTGGTTTTCACGTGCATTGGCTCTCACGAGTATACAATTCTTTGGTGTTTCAA
CACCATTAATTTCAAATNCAAATGTATTAGAAGGTGTTAAAGATAGAGAAG---------
-TAACTTATCAAGATTGGTCAAAATTAATTGAAACAAATTTTGAAAAATATTATTATGTT
CCATCAAGCGGTGATGATAAGAAATATATTATCAATTCAAATTACGTTCATCGTAGATTC
ATTTATAAAGATGTAGTTGGTAGTGCAAACGTTTTCTCTGATTACCAATTAAGACCAAAT
ATTTGTGTTGCAATGTCATTTGCACCAAAGTTATTCAATGATCAACATGCAAATAAATGT
ATTGATATCATTAGAAATTCATTGGTTGGTCCAATCGGTATGAAAACTCTTGATCCAAAT
GATCCAAATTATCATGGTAACTATGATAATTCATCAGATTCTGGTTATAAACCAACATCA
ANAGGTTTCAATTATCATAATGGTCCTGAATGGGTTTGGTGTTATGGTTTCTTTTTGGAA
TCAATTGTACAATTTAAACAGTTCCCTTCGATTTCAACAAAGAATCATTTAATCAATTCA
TTATTAATTAAACATAAACTTTACATTCAAAATGATAAACGTTTCTCATTACCAGAGTTA
ACTAATCGTGAAGGTAGTTTTTGTAAANATAGTTGTGATTCTCAAGCTTGGTCAATTGCA
ACAATTTTAGCTTCACCTTATCAATTATCAATTACTAAAAATTAAATATTCATTTTTTTA
TANATATGTATATATTAAAAAAAAAAGCCAACTTTTTTAAACAC
Length of connected seq. 1294
Full length Seq ID -
Full length Seq. -
Length of full length seq. -