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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CHD189
Library CH
(Link to library)
Clone ID CHD189
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16560-1
Original site URL
Representative seq. ID CHD189P
(Link to Original site)
Representative DNA sequence 
>CHD189 (CHD189Q) /CSM/CH/CHD1-D/CHD189Q.Seq.d/
ATTTTTATTATACAAATATTAAATTCATATTTTATTTTTTTATATATTGTAACATTTCAT
AAAAAAAAAAAAAAAAAAAAAAANTTAAAAANNCNCNTTTTTTGAAAAAAAAAAXXXXXX
XXXXAANACCNTCCCGGTAAGACTNTNCCCTGGAAGTNAAGGTAGTGCCACATTGAGAAT
GTAAAACAAAGATNCAGATAAAGAAGGTATNCCNCCAGATCANCAACGTNTCATTTTCGC
TGGTAAACAATTANAANATGGTCGTACTCTCTCTGATTACAATATCCAAAAAAGAATCAA
CTCTCCACTTGGTCCTCAGATTAAGAGGTGGTATGCAAATCTTTGTCAAANCATTAACTG
GTAAGACCATCACTCTTGAAGTTGAGGGCTCAGATAATATTGAAAATGTTAAAACAAAGA
TTCAAGACAAAGAAGGTATCCCACCAGACCAACAAAGATTAATTTTCGCTGGTAAACAAT
TAGAAGATGGTCGTACTCTCTCTGATTACAACATTCAAAAGGAATCCACTCTCCATTTAG
TCCTCAGATTAAGAGGTGGTATGCAAATTTTTGTCAAAACCCTCACTGGTAAGACTATTA
CACTCGAAGTTGAAGGTTCTGATAACATTGAGAATGTTAAAGCCAAAATCCAAGACAAAG
AAGGTATTCCACCAGATCAACAACGTCTCATTTTCGCCGGTAAACAATTGGAGGATGGTC
GTACTCTCTCTGATTACAACATTCAAAAGGAATCCACTCTCCATTTAGTTCTCAGATTAA
GAGGTGGTATGCAAATTTTTGTAAAAACTCTCACTGGTAAAACCATTACACTCGAAGTTG
AAGGTAGTGACAACATTGAGAATGTAAAAACAAAAATTCAAGACAAAGAAGGTATCCCAC
CAGNT
sequence update 2002.10.25
Translated Amino Acid sequence 
fyytnikfifyffiycnis*KKKKKKKXKXXXFLKKK---

---XTXPVRLXPGSXGSATLRM*nkdxdkegxppdxqrxifagkqlxxgrtlsdyniqkr
insplgpqikrwyanlcqxinw*dhhs*s*glr*y*kc*nkdsrqrryptrptkinfrw*
tirrwsysl*lqhskgihspfspqikrwyanfcqnphw*dyytrs*rf**h*ec*sqnpr
qrrystrsttshfrr*tiggwsysl*lqhskgihspfssqikrwyanfcknshw*nhytr
s*r**qh*ecknknsrqrryptx
Translated Amino Acid sequence (All Frames) 
Frame A:
ifiiqilnsyfiflyivtfhkkkkkkkxlkxxxf*kkk---

---XTXPVRLXPGSXGSATLRM*nkdxdkegxppdxqrxifagkqlxxgrtlsdyniqkr
insplgpqikrwyanlcqxinw*dhhs*s*glr*y*kc*nkdsrqrryptrptkinfrw*
tirrwsysl*lqhskgihspfspqikrwyanfcqnphw*dyytrs*rf**h*ec*sqnpr
qrrystrsttshfrr*tiggwsysl*lqhskgihspfssqikrwyanfcknshw*nhytr
s*r**qh*ecknknsrqrryptx

Frame B:
fllyky*ihilffyil*hfikkkkkkkx*kxxffekk---

---xpsr*dxxlevkvvph*ecktkxqikkvxxqixnvsfslvnnxxmvvlslitiskke
stlhlvlrlrggmqifvkxltgktitlevegsdnienvktkiqdkegippdqqrlifagk
qledgrtlsdyniqkestlhlvlrlrggmqifvktltgktitlevegsdnienvkakiqd
kegippdqqrlifagkqledgrtlsdyniqkestlhlvlrlrggmqifvktltgktitle
vegsdnienvktkiqdkegippx

Frame C:
fyytnikfifyffiycnis*KKKKKKKXKXXXFLKKK---

---xxpgktxpwkxr*chienvkqrxr*rryxxrsxtxhfrw*tixxwsysl*lqypkkn
qlstwssd*evvckslsxh*lvrpsllklraqiilkmlkqrfktkkvshqtnkd*fslvn
n*kmvvlslittfkrnplsi*ssd*evvckflskpslvrllhsklkvlitlrmlkpkskt
kkvfhqinnvsfspvnnwrmvvlslittfkrnplsi*fsd*evvckfl*klslvkplhsk
lkvvttlrm*kqkfktkkvshq
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHD189 (CHD189Q) /CSM/CH/CHD1-D/CHD189Q.Seq.d/                       1467   0.0
SFC896 (SFC896Q) /CSM/SF/SFC8-D/SFC896Q.Seq.d/                       1380   0.0
CFC718 (CFC718Q) /CSM/CF/CFC7-A/CFC718Q.Seq.d/                       1380   0.0
CFC580 (CFC580Q) /CSM/CF/CFC5-D/CFC580Q.Seq.d/                       1380   0.0
CFG543 (CFG543Q) /CSM/CF/CFG5-B/CFG543Q.Seq.d/                       1376   0.0
SFF769 (SFF769Q) /CSM/SF/SFF7-C/SFF769Q.Seq.d/                       1372   0.0
CFG340 (CFG340Q) /CSM/CF/CFG3-B/CFG340Q.Seq.d/                       1370   0.0
SFD545 (SFD545Q) /CSM/SF/SFD5-B/SFD545Q.Seq.d/                       1368   0.0
SFD191 (SFD191Q) /CSM/SF/SFD1-D/SFD191Q.Seq.d/                       1366   0.0
AFD482 (AFD482Q) /CSM/AF/AFD4-D/AFD482Q.Seq.d/                       1366   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M19491|M19491.1 Slime mold (D.discoideum) ubiquitin p229C/pLK229 gene, complete cds.  1205   0.0    3
M23748|M23748.1 D.discoideum ubiquitin mRNA, complete cds, clone DCUB19.  1189   0.0    4
M19666|M19666.1 Slime mold (D.discoideum) ubiquitin gene, 3' end, clone p229C.  1189   0.0    3
M23753|M23753.1 D.discoideum ubiquitin mRNA, complete cds, clone DCUB14.   690   0.0    3
M23754|M23754.1 D.discoideum ubiquitin mRNA, complete cds, clone DCUB2.   674   0.0    3
M19492|M19492.1 Slime mold (D.discoideum) ubiquitin lambda-229 gene, complete cds.   658   0.0    3
M23752|M23752.1 D.discoideum ubiquitin mRNA, complete cds, clone DCUB17.   533   0.0    3
M23751|M23751.1 D.discoideum ubiquitin mRNA, complete cds, clone DCUB18.   533   0.0    3
Z54197|Z54197.1 C.albicans UBI4 gene encoding polyubiquitin.      170   3e-99  5
BJ541175|BJ541175.1 Oryzias latipes cDNA clone:MF01SSB034A18, 3' end single read.   176   3e-96  5
dna update 2004. 9.18
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

A27806(A27806) polyubiquitin 5 (clone pLK229) - slime mold (Dict...   396   e-119
M19666_1(M19666|pid:none) Slime mold (D.discoideum) ubiquitin ge...   396   e-119
B34080(B34080)polyubiquitin 5 (clone DCUB19) - slime mold (Dicty...   396   e-119
M23754_1(M23754|pid:none) D.discoideum ubiquitin mRNA, complete ...   392   e-118
AY957885_1(AY957885|pid:none) Gracilaria lemaneiformis polyubiqu...   381   e-115
AB021974_1(AB021974|pid:none) Bombyx mori UBI3 mRNA for polyubiq...   379   e-114
T16144(T16144) ubiquitin - Caenorhabditis elegans  &U23172_1(U23...   379   e-114
S53719(S53719;S73107;S71069)polyubiquitin 6 - red alga (Gracilar...   379   e-114
S32020(S32020) polyubiquitin 6 - Geodia cydonium  &X70917_1(X709...   379   e-114
Y12081_1(Y12081|pid:none) Suberites domuncula mRNA for polyubiqu...   379   e-114
protein update 2009. 3.30
PSORT 

 psg: 0.55  gvh: 0.49  alm: 0.60  top: 0.53  tms: 0.00  mit: 0.45  mip: 0.00
 nuc: 0.60  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 76.0 %: nuclear
	  8.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system
	  4.0 %: cytoplasmic

>> prediction for CHD189 is nuc
5' end seq. ID CHD189F
5' end seq. 
>CHD189F.Seq
ATTTTTATTATACAAATATTAAATTCATATTTTATTTTTTTATATATTGTAACATTTCAT
AAAAAAAAAAAAAAAAAAAAAAANTTAAAAANNCNCNTTTTTTGAAAAAAAAAANNNNNN
NNNN
Length of 5' end seq. 124
3' end seq. ID CHD189Z
3' end seq. 
>CHD189Z.Seq
NNNNNNNNNNAANACCNTCCCGGTAAGACTNTNCCCTGGAAGTNAAGGTAGTGCCACATT
GAGAATGTAAAACAAAGATNCAGATAAAGAAGGTATNCCNCCAGATCANCAACGTNTCAT
TTTCGCTGGTAAACAATTANAANATGGTCGTACTCTCTCTGATTACAATATCCAAAAAAG
AATCAACTCTCCACTTGGTCCTCAGATTAAGAGGTGGTATGCAAATCTTTGTCAAANCAT
TAACTGGTAAGACCATCACTCTTGAAGTTGAGGGCTCAGATAATATTGAAAATGTTAAAA
CAAAGATTCAAGACAAAGAAGGTATCCCACCAGACCAACAAAGATTAATTTTCGCTGGTA
AACAATTAGAAGATGGTCGTACTCTCTCTGATTACAACATTCAAAAGGAATCCACTCTCC
ATTTAGTCCTCAGATTAAGAGGTGGTATGCAAATTTTTGTCAAAACCCTCACTGGTAAGA
CTATTACACTCGAAGTTGAAGGTTCTGATAACATTGAGAATGTTAAAGCCAAAATCCAAG
ACAAAGAAGGTATTCCACCAGATCAACAACGTCTCATTTTCGCCGGTAAACAATTGGAGG
ATGGTCGTACTCTCTCTGATTACAACATTCAAAAGGAATCCACTCTCCATTTAGTTCTCA
GATTAAGAGGTGGTATGCAAATTTTTGTAAAAACTCTCACTGGTAAAACCATTACACTCG
AAGTTGAAGGTAGTGACAACATTGAGAATGTAAAAACAAAAATTCAAGACAAAGAAGGTA
TCCCACCAGNT
Length of 3' end seq. 791
Connected seq. ID CHD189P
Connected seq. 
>CHD189P.Seq
ATTTTTATTATACAAATATTAAATTCATATTTTATTTTTTTATATATTGTAACATTTCAT
AAAAAAAAAAAAAAAAAAAAAAANTTAAAAANNCNCNTTTTTTGAAAAAAAAAA------
----AANACCNTCCCGGTAAGACTNTNCCCTGGAAGTNAAGGTAGTGCCACATTGAGAAT
GTAAAACAAAGATNCAGATAAAGAAGGTATNCCNCCAGATCANCAACGTNTCATTTTCGC
TGGTAAACAATTANAANATGGTCGTACTCTCTCTGATTACAATATCCAAAAAAGAATCAA
CTCTCCACTTGGTCCTCAGATTAAGAGGTGGTATGCAAATCTTTGTCAAANCATTAACTG
GTAAGACCATCACTCTTGAAGTTGAGGGCTCAGATAATATTGAAAATGTTAAAACAAAGA
TTCAAGACAAAGAAGGTATCCCACCAGACCAACAAAGATTAATTTTCGCTGGTAAACAAT
TAGAAGATGGTCGTACTCTCTCTGATTACAACATTCAAAAGGAATCCACTCTCCATTTAG
TCCTCAGATTAAGAGGTGGTATGCAAATTTTTGTCAAAACCCTCACTGGTAAGACTATTA
CACTCGAAGTTGAAGGTTCTGATAACATTGAGAATGTTAAAGCCAAAATCCAAGACAAAG
AAGGTATTCCACCAGATCAACAACGTCTCATTTTCGCCGGTAAACAATTGGAGGATGGTC
GTACTCTCTCTGATTACAACATTCAAAAGGAATCCACTCTCCATTTAGTTCTCAGATTAA
GAGGTGGTATGCAAATTTTTGTAAAAACTCTCACTGGTAAAACCATTACACTCGAAGTTG
AAGGTAGTGACAACATTGAGAATGTAAAAACAAAAATTCAAGACAAAGAAGGTATCCCAC
CAGNT
Length of connected seq. 895
Full length Seq ID -
Full length Seq. -
Length of full length seq. -