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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CHD185
Library CH
(Link to library)
Clone ID CHD185
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U11674-1
Original site URL
Representative seq. ID CHD185P
(Link to Original site)
Representative DNA sequence 
>CHD185 (CHD185Q) /CSM/CH/CHD1-D/CHD185Q.Seq.d/
TAAAATATTCAAATATTATGTTTTTTAGATATTTTTCAATTTTAATTCAAGAATTTGATA
TAAATTTAGATGAAGCATCAATTTTAAATGCATTATCATTTATAAATATAAATTTGAATA
GTTTAAATGAACATTTTACACTTCATCCAACAATTACACAAGAAGAAATTCTAGAAACAA
AGAATGCATCCAATATAGGGAATCATATGGTTTACTTTGAAATGTTACATATTAATCCAG
TTAAAATGAATTTATCATTCATTAGTTGTAAGAGTCCAAAAGAAACTCAAGCAATTCTTG
GTGCTAGATCATTGGCTGAACTATTGATTGGTTTTAAATCAAATTCACCCTTTTTAAATA
TTGAAAGAGCACCCATCAAATTCAATGGTTTCATTTGGGAACATCCATTCCTCTCGACTA
GACAAGTTATCGATGAAATATCATTACATTTCTCTTATCAAATGATGAGTCAAGCTCATA
AAATTTTTGGTTCATTCGATTTCATTGGTAATCCAATTAGATTGGCCGAATCGTTAGGTA
GTGGTTTTAAAGATTTTTTCCATGAACCTGCATTAGGTTTAGTTAAAAGTCCTCAAGATT
TTGCTGCTGGTCTCTCAAAAGGXXXXXXXXXXATAGTTATATAAAAGAAAGCCAAGAAAG
TAATAACCAAAAACCAAAAGGAGTTAAAGAAGGTTTAGAATTTGGTTTCCGTGATTTTGG
TGAGGGTGTTATTAAAGGAATTACTGGTATCATCGATGAACCCTACAAAGGTGCAACACA
AGAGAAATCTTGGGAAGGTTTTTTCAAAGGTATTGGTAAAGGTGTTTTGGGTGTGGCAGT
TAAACCAACGGTTGGAGTTTTCGATTTAGTTAGTAAAACATCTGAAGGTATTAAAAATTC
AACCACTGTTGCAAAATCACTCTCTCAAATTAAAAGACGTCGTATTCCAAGATATTTCCC
AAGAGAAGGTACATTATCAACCTATAATCAATTTAAATCCATTGGTTCCTTTATACTTTA
TTCAAAGATTGGTCCACCACCAACTCATGATTGGTATGTTTTTCATACTATTCTAAACTA
TAAAGATTTGATTTTAATTGGTAGCAATTATCATCTATTGTTAATTCGTTGTGCAAATCT
TTCAATAAATGGTAATTTATCGGATTCTGAAATCATTTGGAAAGTTAAATTCTCTGAAAT
CATTCAAATTAAAAATATACCTTCCAATTTTGGTTTCCAATTATTTGTAAATGTAAATGG
TGTTGGTCAACCTCCAATCACTCTTTTAATTCCAACTCTAACTGAAGAAGTAAGAAATAG
TGTTGTTAGAAAAATTACNATTTAATTTTGTNGAAAAAAGATTGGTAATAATTTAGATTA
GAAAATAAAAAAAATAAAATTTAANAA
sequence update 2002.10.25
Translated Amino Acid sequence 
KYSNIMFFRYFSILIQEFDINLDEASILNALSFININLNSLNEHFTLHPTITQEEILETK
NASNIGNHMVYFEMLHINPVKMNLSFISCKSPKETQAILGARSLAELLIGFKSNSPFLNI
ERAPIKFNGFIWEHPFLSTRQVIDEISLHFSYQMMSQAHKIFGSFDFIGNPIRLAESLGS
GFKDFFHEPALGLVKSPQDFAAGLSK---

---SYIKESQESNNQKPKGVKEGLEFGFRDFGEGVIKGITGIIDEPYKGATQEKSWEGFF
KGIGKGVLGVAVKPTVGVFDLVSKTSEGIKNSTTVAKSLSQIKRRRIPRYFPREGTLSTY
NQFKSIGSFILYSKIGPPPTHDWYVFHTILNYKDLILIGSNYHLLLIRCANLSINGNLSD
SEIIWKVKFSEIIQIKNIPSNFGFQLFVNVNGVGQPPITLLIPTLTEEVRNSVVRKITI*
fcxkkignnld*kikkikfx
Translated Amino Acid sequence (All Frames) 
Frame A:
*niqilcfldifqf*fknli*i*mkhqf*mhyhl*i*i*iv*mnilhfiqqlhkkkf*kq
rmhpi*giiwftlkcyiliqlk*iyhslvvrvqkklkqflvldhwlny*lvlnqihpf*i
lkehpsnsmvsfgnihssrldklsmkyhyislik**vklikflvhsislviqldwpnr*v
vvlkifsmnlh*v*lkvlkilllvsqk---

---ivi*kkakkvitknqkelkkv*nlvsvilvrvllkellvssmnptkvqhkrnlgkvf
skvlvkvfwvwqlnqrlefsi*lvkhlkvlkiqpllqnhslklkdvvfqdisqekvhyqp
iinlnplvplyfiqrlvhhqlmigmffilf*tiki*f*lvaiiiyc*fvvqifq*mviyr
ilksfgklnslksfklkiylpilvsnyl*m*mvlvnlqslf*fql*lkk*eivlleklxf
nfvekrlvii*irk*kk*nlx

Frame B:
kifkyyvf*iffnfnsri*ykfr*sinfkciiiykykfe*fk*tfytssnnytrrnsrnk
eciqyresygll*nvty*ss*nefiih*l*eskrnssnswc*iig*tidwf*ikftlfky
*ksthqiqwfhlgtsipld*tsyr*niitfllsndesss*nfwfirfhw*sn*igrivr*
wf*rffp*tcirfs*kssrfccwslkr---

---*lykrkprk**pktkrs*rrfriwfp*fw*gcy*rnywyhr*tlqrcntreilgrff
qryw*rcfgcgs*tngwsfrfs**ni*ry*kfnhcckitlsn*ktsyskifpkrryiinl
*si*ihwflytlfkdwsttns*lvcfsyyskl*rfdfnw*qlssivnslcksfnkw*fig
f*nhles*il*nhsn*kytfqfwfpiickckwcwstsnhsfnsnsn*rskk*cc*knyxl
ilxkkdw**frlenkknki*x

Frame C:
KYSNIMFFRYFSILIQEFDINLDEASILNALSFININLNSLNEHFTLHPTITQEEILETK
NASNIGNHMVYFEMLHINPVKMNLSFISCKSPKETQAILGARSLAELLIGFKSNSPFLNI
ERAPIKFNGFIWEHPFLSTRQVIDEISLHFSYQMMSQAHKIFGSFDFIGNPIRLAESLGS
GFKDFFHEPALGLVKSPQDFAAGLSK---

---SYIKESQESNNQKPKGVKEGLEFGFRDFGEGVIKGITGIIDEPYKGATQEKSWEGFF
KGIGKGVLGVAVKPTVGVFDLVSKTSEGIKNSTTVAKSLSQIKRRRIPRYFPREGTLSTY
NQFKSIGSFILYSKIGPPPTHDWYVFHTILNYKDLILIGSNYHLLLIRCANLSINGNLSD
SEIIWKVKFSEIIQIKNIPSNFGFQLFVNVNGVGQPPITLLIPTLTEEVRNSVVRKITI*
fcxkkignnld*kikkikfx
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHD185 (CHD185Q) /CSM/CH/CHD1-D/CHD185Q.Seq.d/                       2666   0.0
CHF542 (CHF542Q) /CSM/CH/CHF5-B/CHF542Q.Seq.d/                       1451   0.0
CHM410 (CHM410Q) /CSM/CH/CHM4-A/CHM410Q.Seq.d/                       1388   0.0
SFI107 (SFI107Q) /CSM/SF/SFI1-A/SFI107Q.Seq.d/                       1316   0.0
SLE389 (SLE389Q) /CSM/SL/SLE3-D/SLE389Q.Seq.d/                         46   0.001
SFL414 (SFL414Q) /CSM/SF/SFL4-A/SFL414Q.Seq.d/                         46   0.001
VHL770 (VHL770Q) /CSM/VH/VHL7-C/VHL770Q.Seq.d/                         40   0.090
SHI787 (SHI787Q) /CSM/SH/SHI7-D/SHI787Q.Seq.d/                         40   0.090
SHF595 (SHF595Q) /CSM/SH/SHF5-D/SHF595Q.Seq.d/                         40   0.090
SFA361 (SFA361Q) /CSM/SF/SFA3-C/SFA361Q.Seq.d/                         40   0.090
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AF079445|AF079445.1 Dictyostelium discoideum TipC (tipC) gene, complete cds.  1384   0.0    2
AC116982|AC116982.2 Dictyostelium discoideum chromosome 2 map 3622643-3879522 strain AX4, complete sequence.    34   7e-10  18
AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.    40   1e-09  19
AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.    46   3e-08  20
AE017339|AE017339.1 Mycoplasma mobile 163K section 3 of 3 of...    34   7e-08  20
AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.    34   6e-07  22
AE017338|AE017338.1 Mycoplasma mobile 163K section 2 of 3 of...    34   8e-07  20
AE017337|AE017337.1 Mycoplasma mobile 163K section 1 of 3 of...    38   1e-06  19
BX842643|BX842643.1 Mycoplasma mycoides subsp. mycoides SC genomic DNA, complete  sequence; segment 2/4.    36   2e-06  21
AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.    34   2e-06  26
dna update 2004. 9.18
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AF079445_1(AF079445|pid:none) Dictyostelium discoideum TipC (tip...   476   0.0
(Q54LB8) RecName: Full=Putative vacuolar protein sorting-associa...   114   2e-50
(Q54LN2) RecName: Full=Putative vacuolar protein sorting-associa...   116   3e-50
AP007155_926(AP007155|pid:none) Aspergillus oryzae RIB40 genomic...   105   1e-40
AE017343_227(AE017343|pid:none) Cryptococcus neoformans var. neo...   100   6e-38
(Q9H995) RecName: Full=Vacuolar protein sorting-associated prote...    98   2e-35
AF337532_1(AF337532|pid:none) Homo sapiens chorea-acanthocytosis...    98   2e-35
AJ608769_1(AJ608769|pid:none) Homo sapiens mRNA for chorein 2A (...    98   2e-35
BC163680_1(BC163680|pid:none) Danio rerio vacuolar protein sorti...    99   4e-35
BC062130_1(BC062130|pid:none) Mus musculus vacuolar protein sort...    99   5e-35
protein update 2009. 3.30
PSORT 

 psg: 0.76  gvh: 0.43  alm: 0.36  top: 0.53  tms: 0.00  mit: 0.33  mip: 0.03
 nuc: 0.04  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 36.0 %: nuclear
	  8.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: peroxisomal

>> prediction for CHD185 is cyt
5' end seq. ID CHD185F
5' end seq. 
>CHD185F.Seq
TAAAATATTCAAATATTATGTTTTTTAGATATTTTTCAATTTTAATTCAAGAATTTGATA
TAAATTTAGATGAAGCATCAATTTTAAATGCATTATCATTTATAAATATAAATTTGAATA
GTTTAAATGAACATTTTACACTTCATCCAACAATTACACAAGAAGAAATTCTAGAAACAA
AGAATGCATCCAATATAGGGAATCATATGGTTTACTTTGAAATGTTACATATTAATCCAG
TTAAAATGAATTTATCATTCATTAGTTGTAAGAGTCCAAAAGAAACTCAAGCAATTCTTG
GTGCTAGATCATTGGCTGAACTATTGATTGGTTTTAAATCAAATTCACCCTTTTTAAATA
TTGAAAGAGCACCCATCAAATTCAATGGTTTCATTTGGGAACATCCATTCCTCTCGACTA
GACAAGTTATCGATGAAATATCATTACATTTCTCTTATCAAATGATGAGTCAAGCTCATA
AAATTTTTGGTTCATTCGATTTCATTGGTAATCCAATTAGATTGGCCGAATCGTTAGGTA
GTGGTTTTAAAGATTTTTTCCATGAACCTGCATTAGGTTTAGTTAAAAGTCCTCAAGATT
TTGCTGCTGGTCTCTCAAAAGGNNNNNNNNNN
Length of 5' end seq. 632
3' end seq. ID CHD185Z
3' end seq. 
>CHD185Z.Seq
NNNNNNNNNNATAGTTATATAAAAGAAAGCCAAGAAAGTAATAACCAAAAACCAAAAGGA
GTTAAAGAAGGTTTAGAATTTGGTTTCCGTGATTTTGGTGAGGGTGTTATTAAAGGAATT
ACTGGTATCATCGATGAACCCTACAAAGGTGCAACACAAGAGAAATCTTGGGAAGGTTTT
TTCAAAGGTATTGGTAAAGGTGTTTTGGGTGTGGCAGTTAAACCAACGGTTGGAGTTTTC
GATTTAGTTAGTAAAACATCTGAAGGTATTAAAAATTCAACCACTGTTGCAAAATCACTC
TCTCAAATTAAAAGACGTCGTATTCCAAGATATTTCCCAAGAGAAGGTACATTATCAACC
TATAATCAATTTAAATCCATTGGTTCCTTTATACTTTATTCAAAGATTGGTCCACCACCA
ACTCATGATTGGTATGTTTTTCATACTATTCTAAACTATAAAGATTTGATTTTAATTGGT
AGCAATTATCATCTATTGTTAATTCGTTGTGCAAATCTTTCAATAAATGGTAATTTATCG
GATTCTGAAATCATTTGGAAAGTTAAATTCTCTGAAATCATTCAAATTAAAAATATACCT
TCCAATTTTGGTTTCCAATTATTTGTAAATGTAAATGGTGTTGGTCAACCTCCAATCACT
CTTTTAATTCCAACTCTAACTGAAGAAGTAAGAAATAGTGTTGTTAGAAAAATTACNATT
TAATTTTGTNGAAAAAAGATTGGTAATAATTTAGATTAGAAAATAAAAAAAATAAAATTT
AANAA
Length of 3' end seq. 785
Connected seq. ID CHD185P
Connected seq. 
>CHD185P.Seq
TAAAATATTCAAATATTATGTTTTTTAGATATTTTTCAATTTTAATTCAAGAATTTGATA
TAAATTTAGATGAAGCATCAATTTTAAATGCATTATCATTTATAAATATAAATTTGAATA
GTTTAAATGAACATTTTACACTTCATCCAACAATTACACAAGAAGAAATTCTAGAAACAA
AGAATGCATCCAATATAGGGAATCATATGGTTTACTTTGAAATGTTACATATTAATCCAG
TTAAAATGAATTTATCATTCATTAGTTGTAAGAGTCCAAAAGAAACTCAAGCAATTCTTG
GTGCTAGATCATTGGCTGAACTATTGATTGGTTTTAAATCAAATTCACCCTTTTTAAATA
TTGAAAGAGCACCCATCAAATTCAATGGTTTCATTTGGGAACATCCATTCCTCTCGACTA
GACAAGTTATCGATGAAATATCATTACATTTCTCTTATCAAATGATGAGTCAAGCTCATA
AAATTTTTGGTTCATTCGATTTCATTGGTAATCCAATTAGATTGGCCGAATCGTTAGGTA
GTGGTTTTAAAGATTTTTTCCATGAACCTGCATTAGGTTTAGTTAAAAGTCCTCAAGATT
TTGCTGCTGGTCTCTCAAAAGG----------ATAGTTATATAAAAGAAAGCCAAGAAAG
TAATAACCAAAAACCAAAAGGAGTTAAAGAAGGTTTAGAATTTGGTTTCCGTGATTTTGG
TGAGGGTGTTATTAAAGGAATTACTGGTATCATCGATGAACCCTACAAAGGTGCAACACA
AGAGAAATCTTGGGAAGGTTTTTTCAAAGGTATTGGTAAAGGTGTTTTGGGTGTGGCAGT
TAAACCAACGGTTGGAGTTTTCGATTTAGTTAGTAAAACATCTGAAGGTATTAAAAATTC
AACCACTGTTGCAAAATCACTCTCTCAAATTAAAAGACGTCGTATTCCAAGATATTTCCC
AAGAGAAGGTACATTATCAACCTATAATCAATTTAAATCCATTGGTTCCTTTATACTTTA
TTCAAAGATTGGTCCACCACCAACTCATGATTGGTATGTTTTTCATACTATTCTAAACTA
TAAAGATTTGATTTTAATTGGTAGCAATTATCATCTATTGTTAATTCGTTGTGCAAATCT
TTCAATAAATGGTAATTTATCGGATTCTGAAATCATTTGGAAAGTTAAATTCTCTGAAAT
CATTCAAATTAAAAATATACCTTCCAATTTTGGTTTCCAATTATTTGTAAATGTAAATGG
TGTTGGTCAACCTCCAATCACTCTTTTAATTCCAACTCTAACTGAAGAAGTAAGAAATAG
TGTTGTTAGAAAAATTACNATTTAATTTTGTNGAAAAAAGATTGGTAATAATTTAGATTA
GAAAATAAAAAAAATAAAATTTAANAA
Length of connected seq. 1397
Full length Seq ID -
Full length Seq. -
Length of full length seq. -